Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F488 I0 R1	1275	300.1	10328444	91.0%	9398884	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,246,619	Δ1 bp	94.4%	coding (350/357 nt)	yhaI →	DUF805 family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,246,619	0	C	.	94.4%	1010.8 / 52.1	214	coding (350/357 nt)	yhaI	DUF805 family inner membrane protein
Reads supporting (aligned to +/- strand): ref base C (7/5); new base . (92/110); total (99/115)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 217 total aligned reads displayed.

ATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAAT  >  CP009273/3246471‑3246763

aTCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCa                                                                                                                                                 >  2:3212750/1‑150 (MQ=255)

 tCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTaaa                                                                                                                                                >  2:4216587/1‑150 (MQ=255)

  ccGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCaaa                                                                                                                                               >  1:4074673/1‑150 (MQ=255)

  ccGTCGCGTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTGTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTaaat                                                                                                                                               <  1:3279629/150‑2 (MQ=255)

   cGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAAtt                                                                                                                                             <  1:4910819/150‑1 (MQ=255)

   cGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAAtt                                                                                                                                             <  2:1120800/150‑1 (MQ=255)

   cGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAAtt                                                                                                                                             >  2:3713602/1‑150 (MQ=255)

    gTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTa                                                                                                                                            <  2:1547220/150‑1 (MQ=255)

    gTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTa                                                                                                                                            <  1:3102437/150‑1 (MQ=255)

       gCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATc                                                                                                                                         <  2:508746/150‑1 (MQ=255)

           acacGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAg                                                                                                                                     <  1:3041261/150‑1 (MQ=255)

                  accgaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTggg                                                                                                                              <  1:1160278/150‑1 (MQ=255)

                  accgaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTggg                                                                                                                              >  2:3441603/1‑150 (MQ=255)

                    cgaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGc                                                                                                                            <  1:3881611/150‑1 (MQ=255)

                     gaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCg                                                                                                                           >  1:3224788/1‑150 (MQ=255)

                     gaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCg                                                                                                                           <  2:2891191/150‑1 (MQ=255)

                     gaccgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCg                                                                                                                           <  1:1126726/150‑1 (MQ=255)

                      accgTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGa                                                                                                                          <  1:492931/150‑1 (MQ=255)

                         gTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGatat                                                                                                                       >  2:881467/1‑150 (MQ=255)

                         gTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGatat                                                                                                                       >  2:171977/1‑150 (MQ=255)

                         gTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGatat                                                                                                                       >  2:1867405/1‑150 (MQ=255)

                           tCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATAttt                                                                                                                     >  2:2192684/1‑150 (MQ=255)

                           tCAGGTGCCTGGGCCTTGCTCTTTTTTGGTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATAtt                                                                                                                      <  2:501212/150‑1 (MQ=255)

                            cAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTc                                                                                                                    <  2:3041967/150‑1 (MQ=255)

                              ggTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGc                                                                                                                  <  2:4163348/150‑1 (MQ=255)

                               gTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGcc                                                                                                                 <  2:4918968/150‑1 (MQ=255)

                                tGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGccc                                                                                                                <  2:4754362/150‑1 (MQ=255)

                                   cTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtt                                                                                                             <  1:4578429/150‑1 (MQ=255)

                                   cTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtt                                                                                                             <  1:4277144/150‑1 (MQ=255)

                                   cTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtt                                                                                                             >  1:4088392/1‑150 (MQ=255)

                                   cTGGGCCTTGCTCTTTTTTGTTCCGTTTATAGGCTGGCACGTGATTCTGGTTTTTTTCTGCAAAGAAGGTACTTATGGCAGCAATCGTAACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTAAGAATTGGCGCGATATTTCGCCACta                                                                                                             >  2:339526/1‑149 (MQ=255)

                                     gggCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt                                                                                                           >  1:3679158/1‑150 (MQ=255)

                                     gggCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCtttt                                                                                                           <  2:1178036/150‑1 (MQ=255)

                                       gCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCttttt                                                                                                          <  2:3615965/150‑1 (MQ=255)

                                       gCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTtta                                                                                                         <  2:2793107/150‑1 (MQ=255)

                                       gCCTTGCTCTTTTATGTTCCGTTTCTTGGCTGGCTCGTGCTTCTGGTTTTTTACGGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTtta                                                                                                         <  2:5163922/150‑1 (MQ=255)

                                        ccTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttat                                                                                                        >  2:1405937/1‑150 (MQ=255)

                                        ccTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTATTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttat                                                                                                        <  1:3869088/150‑1 (MQ=255)

                                          ttGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTttatta                                                                                                      >  1:179474/1‑150 (MQ=255)

                                            gCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAAc                                                                                                    <  1:1402926/150‑1 (MQ=255)

                                             ctctTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACa                                                                                                   <  1:4182589/150‑1 (MQ=255)

                                                ttttttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACaata                                                                                                >  1:963417/1‑150 (MQ=255)

                                                   tttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAataata                                                                                             >  1:2292198/1‑150 (MQ=255)

                                                   tttGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAataata                                                                                             >  1:3742971/1‑150 (MQ=255)

                                                     tGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATaataaa                                                                                           >  2:461066/1‑150 (MQ=255)

                                                     tGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATaataaa                                                                                           >  2:3839239/1‑150 (MQ=255)

                                                         ccGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAAtatt                                                                                       >  1:1003244/1‑150 (MQ=255)

                                                                 tggctggcTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCg                                                                               >  2:3796990/1‑150 (MQ=255)

                                                                     tggctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTa                                                                            >  1:2264140/1‑150 (MQ=255)

                                                                        ctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaaa                                                                        >  2:4974459/1‑150 (MQ=255)

                                                                        ctCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTaaaaa                                                                        <  2:396673/150‑1 (MQ=255)

                                                                          cGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGt                                                                      >  2:2033192/1‑150 (MQ=255)

                                                                          cGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGt                                                                      <  2:4113331/150‑1 (MQ=255)

                                                                            tGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGttt                                                                    <  2:3521438/150‑1 (MQ=255)

                                                                             gCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGttt                                                                    >  1:4438764/1‑150 (MQ=255)

                                                                               ttCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTggg                                                                 >  1:2416376/1‑150 (MQ=255)

                                                                                tCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGa                                                                >  1:1720080/1‑150 (MQ=255)

                                                                                tCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGa                                                                >  1:4119071/1‑150 (MQ=255)

                                                                                   ggTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAtt                                                             <  1:2061039/150‑1 (MQ=255)

                                                                                    gTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAAttt                                                            >  2:1134765/1‑150 (MQ=255)

                                                                                      ttttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTcc                                                          <  2:860766/150‑1 (MQ=255)

                                                                                       tttttCTGCACAGAAGGTACTTCTGGCAGCAATTGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTccc                                                         >  2:1624745/1‑150 (MQ=255)

                                                                                       tttttCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTccc                                                         <  1:1667416/150‑1 (MQ=255)

                                                                                           tCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAg                                                     >  1:2483292/1‑150 (MQ=255)

                                                                                              gCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGaca                                                  <  2:767326/150‑1 (MQ=255)

                                                                                              gCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGaca                                                  <  1:4693571/150‑1 (MQ=255)

                                                                                                 caGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCa                                               <  2:1207601/150‑1 (MQ=255)

                                                                                                 caGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCa                                               <  1:3723704/150‑1 (MQ=255)

                                                                                                  aGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAg                                              <  2:1949043/150‑1 (MQ=255)

                                                                                                   gAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAgg                                             >  2:16486/1‑150 (MQ=255)

                                                                                                     aGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAt                                           >  1:5055856/1‑150 (MQ=255)

                                                                                                     aGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAt                                           >  2:1311055/1‑150 (MQ=255)

                                                                                                       gTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAttt                                         <  2:303914/150‑1 (MQ=255)

                                                                                                        tACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTc                                        <  2:2474975/150‑1 (MQ=255)

                                                                                                         aCTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCg                                       <  1:5064266/150‑1 (MQ=255)

                                                                                                         aCTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCg                                       >  1:544069/1‑150 (MQ=255)

                                                                                                           ttCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCt                                     <  2:2819343/150‑1 (MQ=255)

                                                                                                             cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTc                                   >  2:3115237/1‑150 (MQ=255)

                                                                                                             cTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTc                                   <  1:3970059/150‑1 (MQ=255)

                                                                                                                 cagcaATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTccccc                               >  1:4919957/1‑150 (MQ=255)

                                                                                                                 cagcaATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTccccc                               <  1:5096203/150‑1 (MQ=255)

                                                                                                                  agcaATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTATAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCa                              >  2:2923371/1‑150 (MQ=255)

                                                                                                                    caATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATa                            <  1:461066/150‑1 (MQ=255)

                                                                                                                     aaTCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATa                            >  1:1808009/1‑150 (MQ=255)

                                                                                                                              ggAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacgac                  >  1:825756/1‑150 (MQ=255)

                                                                                                                              ggAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacgac                  >  2:3073513/1‑150 (MQ=255)

                                                                                                                               gAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCacgacg                 <  1:1108754/150‑1 (MQ=255)

                                                                                                                                 aaCGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGcc               <  1:2878453/150‑1 (MQ=255)

                                                                                                                                  aCGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCa              <  2:4207265/150‑1 (MQ=255)

                                                                                                                                    gATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAgg            >  2:2642297/1‑150 (MQ=255)

                                                                                                                                     aTCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGc           <  2:3443405/150‑1 (MQ=255)

                                                                                                                                      tCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGcc          >  2:4790421/1‑150 (MQ=255)

                                                                                                                                       ccGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCa         >  1:4498572/1‑150 (MQ=255)

                                                                                                                                        cGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCat        >  1:1991602/1‑150 (MQ=255)

                                                                                                                                        cGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATATCCCGCAGACACCAGGCTTTCGCTTCCCCCATACTGTCACTACGCCAGGCCat        >  1:2666014/1‑150 (MQ=255)

                                                                                                                                         gAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAATTTTGGTAATTTCCCGCAGACACCAGGATTTCGCTTCACACATACTGTCACGACTCCAGGCCata       >  1:2327029/1‑150 (MQ=255)

                                                                                                                                         gAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTAATTAACAATAATAAATATTATATTCCGTTAAAAAGTATGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAAACTGTCACGACGCCAGGCCata       <  1:3441603/150‑1 (MQ=255)

                                                                                                                                            gTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataata    >  2:1003007/1‑150 (MQ=255)

                                                                                                                                             tttGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataataa   <  1:3685919/150‑1 (MQ=255)

                                                                                                                                             tttGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCataataa   <  1:1105345/150‑1 (MQ=255)

ATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAAT  >  CP009273/3246471‑3246763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCG  >  CP009273/3246470‑3246767

TATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTAAGGAAAAGATCCGAAGTTTGGTTA                                                                                                                                                      >  SRR19973063.3489919/1‑150 (MQ=60)

   CCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAA                                                                                                                                                   >  SRR19973063.4087729/1‑150 (MQ=60)

   CCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAA                                                                                                                                                   >  SRR19973063.4087792/1‑150 (MQ=60)

   CCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAAT                                                                                                                                                  <  SRR19973063.3289998/150‑1 (MQ=60)

   CCGTCGCGTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTGTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAAT                                                                                                                                                  <  SRR19973063.3290155/150‑1 (MQ=60)

    CGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATT                                                                                                                                                 <  SRR19973063.344785/150‑1 (MQ=60)

    CGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATT                                                                                                                                                 <  SRR19973063.4926677/150‑1 (MQ=60)

     GTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTA                                                                                                                                                <  SRR19973063.3112420/150‑1 (MQ=60)

     GTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTA                                                                                                                                                <  SRR19973063.3111750/150‑1 (MQ=60)

            ACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAG                                                                                                                                         <  SRR19973063.3471164/150‑1 (MQ=60)

            ACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAG                                                                                                                                         <  SRR19973063.3050978/150‑1 (MQ=60)

                   ACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGG                                                                                                                                  <  SRR19973063.1163777/150‑1 (MQ=60)

                   ACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGG                                                                                                                                  <  SRR19973063.1431111/150‑1 (MQ=60)

                    CCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGG                                                                                                                                 <  SRR19973063.4600716/150‑1 (MQ=60)

                    CCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGG                                                                                                                                 <  SRR19973063.492727/150‑1 (MQ=60)

                     CGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGC                                                                                                                                <  SRR19973063.3893924/150‑1 (MQ=60)

                     CGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGC                                                                                                                                <  SRR19973063.3893944/150‑1 (MQ=60)

                      GACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCG                                                                                                                               <  SRR19973063.1130146/150‑1 (MQ=60)

                      GACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCG                                                                                                                               >  SRR19973063.3235148/1‑150 (MQ=60)

                       ACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGA                                                                                                                              <  SRR19973063.494347/150‑1 (MQ=60)

                       ACCGTTCAGGGGCCTGGGCCTTGCTCTTTTTTGTTCCTTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGA                                                                                                                              <  SRR19973063.494248/150‑1 (MQ=60)

                        CCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGAT                                                                                                                             <  SRR19973063.2063222/150‑1 (MQ=60)

                          GTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATAT                                                                                                                           <  SRR19973063.3725476/150‑1 (MQ=60)

                            TCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTT                                                                                                                         >  SRR19973063.4499895/1‑150 (MQ=60)

                             CAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTC                                                                                                                        >  SRR19973063.228061/1‑150 (MQ=60)

                              AGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCG                                                                                                                       >  SRR19973063.61/1‑150 (MQ=60)

                                    CTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTT                                                                                                                 >  SRR19973063.4101493/1‑150 (MQ=60)

                                    CTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTT                                                                                                                 <  SRR19973063.3050246/150‑1 (MQ=60)

                                    CTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTT                                                                                                                 <  SRR19973063.4290740/150‑1 (MQ=60)

                                    CTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTT                                                                                                                 <  SRR19973063.4290790/150‑1 (MQ=60)

                                    CTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTT                                                                                                                 <  SRR19973063.4593075/150‑1 (MQ=60)

                                      GGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTT                                                                                                               >  SRR19973063.3691387/1‑150 (MQ=60)

                                      GGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTT                                                                                                               >  SRR19973063.3690936/1‑150 (MQ=60)

                                         CCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTATTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTAT                                                                                                            <  SRR19973063.3881389/150‑1 (MQ=60)

                                          CTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATT                                                                                                           <  SRR19973063.4816972/150‑1 (MQ=60)

                                          CTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTAGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAAACGTTACGGAAACGATCAGAAGTTTGGTT‑AAATTAATCTTAGAATTGTGTCGATATTTATCCACTTTTTATT                                                                                                           >  SRR19973063.4431410/1‑150 (MQ=60)

                                           TTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTA                                                                                                          >  SRR19973063.180026/1‑150 (MQ=60)

                                             GCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAAC                                                                                                        <  SRR19973063.1407172/150‑1 (MQ=60)

                                             GCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAAC                                                                                                        >  SRR19973063.4638347/1‑150 (MQ=60)

                                             GCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAAC                                                                                                        >  SRR19973063.2828405/1‑150 (MQ=60)

                                              CTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACA                                                                                                       <  SRR19973063.4195945/150‑1 (MQ=60)

                                                 TTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATA                                                                                                    >  SRR19973063.966320/1‑150 (MQ=60)

                                                    TTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATA                                                                                                 >  SRR19973063.2299518/1‑150 (MQ=60)

                                                    TTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATA                                                                                                 >  SRR19973063.3754931/1‑150 (MQ=60)

                                                    TTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATA                                                                                                 >  SRR19973063.2299517/1‑150 (MQ=60)

                                                       GTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAAT                                                                                              >  SRR19973063.4169237/1‑150 (MQ=60)

                                                          CCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATT                                                                                           >  SRR19973063.1006267/1‑150 (MQ=60)

                                                                ATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTC                                                                                     >  SRR19973063.1062427/1‑150 (MQ=60)

                                                                  TGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCG                                                                                   <  SRR19973063.3968312/150‑1 (MQ=60)

                                                                  TGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCG                                                                                   <  SRR19973063.2853499/150‑1 (MQ=60)

                                                                    GCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTT                                                                                 <  SRR19973063.689148/150‑1 (MQ=60)

                                                                      TGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTA                                                                                >  SRR19973063.2271351/1‑150 (MQ=60)

                                                                       GGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAA                                                                              >  SRR19973063.3963159/1‑150 (MQ=60)

                                                                              GCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTT                                                                        >  SRR19973063.4453008/1‑150 (MQ=60)

                                                                                TTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGG                                                                     >  SRR19973063.2562155/1‑150 (MQ=60)

                                                                                TTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGG                                                                     >  SRR19973063.2424101/1‑150 (MQ=60)

                                                                                TTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGG                                                                     >  SRR19973063.2424102/1‑150 (MQ=60)

                                                                                 TCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGA                                                                    <  SRR19973063.4052069/150‑1 (MQ=60)

                                                                                 TCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGA                                                                    >  SRR19973063.4132259/1‑150 (MQ=60)

                                                                                 TCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGA                                                                    >  SRR19973063.1725328/1‑150 (MQ=60)

                                                                                    GGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATT                                                                 <  SRR19973063.2067478/150‑1 (MQ=60)

                                                                                    GGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATT                                                                 <  SRR19973063.2269302/150‑1 (MQ=60)

                                                                                       TTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCC                                                              <  SRR19973063.3823989/150‑1 (MQ=60)

                                                                                        TTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCC                                                             <  SRR19973063.1873240/150‑1 (MQ=60)

                                                                                        TTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCC                                                             <  SRR19973063.1672501/150‑1 (MQ=60)

                                                                                        TTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCC                                                             <  SRR19973063.1672500/150‑1 (MQ=60)

                                                                                         TTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCG                                                            <  SRR19973063.4124666/150‑1 (MQ=60)

                                                                                          TTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGC                                                           >  SRR19973063.4220686/1‑150 (MQ=60)

                                                                                          TTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGC                                                           <  SRR19973063.1111653/150‑1 (MQ=60)

                                                                                            TCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAG                                                         >  SRR19973063.2491224/1‑150 (MQ=60)

                                                                                               GCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACA                                                      <  SRR19973063.4708534/150‑1 (MQ=60)

                                                                                                  CAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCA                                                   <  SRR19973063.1146822/150‑1 (MQ=60)

                                                                                                  CAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCA                                                   <  SRR19973063.3735784/150‑1 (MQ=60)

                                                                                                  CAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCA                                                   <  SRR19973063.3735605/150‑1 (MQ=60)

                                                                                                     AAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGA                                                <  SRR19973063.3539855/150‑1 (MQ=60)

                                                                                                      AGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGAT                                               >  SRR19973063.5072136/1‑150 (MQ=60)

                                                                                                          ACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCG                                           <  SRR19973063.5080563/150‑1 (MQ=60)

                                                                                                          ACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCG                                           <  SRR19973063.5080534/150‑1 (MQ=60)

                                                                                                          ACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCG                                           >  SRR19973063.545679/1‑150 (MQ=60)

                                                                                                            TTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCT                                         <  SRR19973063.2251585/150‑1 (MQ=60)

                                                                                                             TCTGTCAGCAATCGTTTCGGAATCGATCCGTAGTTTGGTTCAAATTAATCTTAGATTTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCT                                         <  SRR19973063.3223079/150‑1 (MQ=60)

                                                                                                             TCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTT                                        >  SRR19973063.4518731/1‑150 (MQ=60)

                                                                                                              CTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTC                                       <  SRR19973063.3982766/150‑1 (MQ=60)

                                                                                                               TGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCC                                      >  SRR19973063.1805404/1‑150 (MQ=60)

                                                                                                                  CATCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCATTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCC                                   <  SRR19973063.5112445/150‑1 (MQ=60)

                                                                                                                  CAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCC                                   >  SRR19973063.4935844/1‑150 (MQ=60)

                                                                                                                  CAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCC                                   <  SRR19973063.5112602/150‑1 (MQ=60)

                                                                                                                  CAGCAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCC                                   >  SRR19973063.4935800/1‑150 (MQ=60)

                                                                                                                     CAATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATA                                <  SRR19973063.462366/150‑1 (MQ=60)

                                                                                                                      AATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATA                                >  SRR19973063.1813616/1‑150 (MQ=60)

                                                                                                                       ATCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACT                              <  SRR19973063.2408586/150‑1 (MQ=60)

                                                                                                                        TCGTTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTG                             >  SRR19973063.98197/1‑150 (MQ=60)

                                                                                                                           TTACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCA                          <  SRR19973063.1794485/150‑1 (MQ=60)

                                                                                                                             ACGGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACG                        >  SRR19973063.1477158/1‑150 (MQ=60)

                                                                                                                               GGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGAC                      <  SRR19973063.4992508/150‑1 (MQ=60)

                                                                                                                               GGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGAC                      >  SRR19973063.828144/1‑150 (MQ=60)

                                                                                                                               GGAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGAC                      >  SRR19973063.1239905/1‑150 (MQ=60)

                                                                                                                                GAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACG                     <  SRR19973063.1112161/150‑1 (MQ=60)

                                                                                                                                GAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACG                     <  SRR19973063.1112125/150‑1 (MQ=60)

                                                                                                                                GAAACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACG                     <  SRR19973063.5119592/150‑1 (MQ=60)

                                                                                                                                  AACGATCCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCC                   <  SRR19973063.2887658/150‑1 (MQ=60)

                                                                                                                                        CCGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCA             >  SRR19973063.4513011/1‑150 (MQ=60)

                                                                                                                                         CGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCAT            >  SRR19973063.1997829/1‑150 (MQ=60)

                                                                                                                                         CGAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATATCCCGCAGACACCAGGCTTTCGCTTCCCCCATACTGTCACTACGCCAGGCCAT            >  SRR19973063.2674570/1‑150 (MQ=60)

                                                                                                                                          GAAGTTTGGTT‑AAATTAATCTTAGAATTGGTGCGATATTTCGCCCCTTTTAATTAACAATAATAAATATTATATTCCGTTAAAAAGTATGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCAAACTGTCACGACGCCAGGCCATA           <  SRR19973063.3452546/150‑1 (MQ=60)

                                                                                                                                          GAAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAATTTTGGTAATTTCCCGCAGACACCAGGATTTCGCTTCACACATACTGTCACGACTCCAGGCCATA           >  SRR19973063.2334479/1‑150 (MQ=60)

                                                                                                                                           AAGTTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAA          >  SRR19973063.885441/1‑150 (MQ=60)

                                                                                                                                              TTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAA       <  SRR19973063.1108709/150‑1 (MQ=60)

                                                                                                                                              TTTGGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAA       <  SRR19973063.3697722/150‑1 (MQ=60)

                                                                                                                                                 GGTT‑AAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATAT    >  SRR19973063.3305843/1‑150 (MQ=60)

                                                                                                                                                    TTAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCG  <  SRR19973063.2517670/150‑1 (MQ=60)

TATCCGTCGCTTACACGATACCGACCGTTCAGGTGCCTGGGCCTTGCTCTTTTTTGTTCCGTTTATTGGCTGGCTCGTGCTTCTGGTTTTTTTCTGCACAGAAGGTACTTCTGGCAGCAATCGTTACGGAAACGATCCGAAGTTTGGTTCAAATTAATCTTAGAATTGGGGCGATATTTCGCCCCTTTTTATTAACAATAATAAATATTATTTTCCGTTAAAAAGTTTGGGAATTTCCCGCAGACACCAGGATTTCGCTTCCCCCATACTGTCACGACGCCAGGCCATAATAATATCG  >  CP009273/3246470‑3246767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`