Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 378,179 A→T 100% pseudogene (150/282 nt) yaiX ← pseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273378,1790AT99.0% 657.4 / ‑2.5 202pseudogene (150/282 nt)yaiXpseudogene, interrupted by IS2A, acetyltransferase homolog; nonfunctional; interruped by IS2; putative transferase
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base T (98/102);  total (99/103)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 213 total aligned reads displayed.

AGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAG  >  CP009273/378034‑378323
                                                                                                                                                 |                                                                                                                                                
aGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAAtttt                                                                                                                                                <  2:1829072/148‑1 (MQ=255)
aGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAAtttt                                                                                                                                                >  1:638257/1‑148 (MQ=255)
 ggggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATtttt                                                                                                                                               >  1:2760019/1‑148 (MQ=255)
 ggggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATtttt                                                                                                                                               <  1:2962403/148‑1 (MQ=255)
 ggggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATtttt                                                                                                                                               <  1:3251373/148‑1 (MQ=255)
  gggCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAAtttttt                                                                                                                                              <  2:429573/148‑1 (MQ=255)
    gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTtc                                                                                                                                            >  2:1301713/1‑148 (MQ=255)
    gCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTtc                                                                                                                                            <  2:2333983/148‑1 (MQ=255)
     cAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTtct                                                                                                                                           <  1:1796750/148‑1 (MQ=255)
         tCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCttt                                                                                                                                       >  2:1753100/1‑148 (MQ=255)
          ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTa                                                                                                                                      <  2:745510/148‑1 (MQ=255)
          ccAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTa                                                                                                                                      <  2:2164529/148‑1 (MQ=255)
           cAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTaa                                                                                                                                     <  1:1899144/148‑1 (MQ=255)
            aaTTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAAc                                                                                                                                    >  1:1845952/1‑148 (MQ=255)
            aaTTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAAc                                                                                                                                    <  2:2819809/148‑1 (MQ=255)
             aTTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCTATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACa                                                                                                                                   <  1:3074442/148‑1 (MQ=255)
               tACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACAtt                                                                                                                                 <  2:1932004/148‑1 (MQ=255)
                aCCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTa                                                                                                                                <  2:314588/148‑1 (MQ=255)
                 ccTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTaa                                                                                                                               >  2:3299760/1‑148 (MQ=255)
                 ccTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACATGCAGTTTCATCAATAATTACCTGGTCTGCAATATGAATTTTTTTCTCTTTAACATTaa                                                                                                                               >  1:1816977/1‑148 (MQ=255)
                   tATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGc                                                                                                                             >  1:1342054/1‑148 (MQ=255)
                      cAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTc                                                                                                                          <  1:2427920/148‑1 (MQ=255)
                           aGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATa                                                                                                                     <  2:2954615/148‑1 (MQ=255)
                            gTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAg                                                                                                                    >  2:76449/1‑148 (MQ=255)
                              ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGtt                                                                                                                  <  1:754778/148‑1 (MQ=255)
                              ttGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACATTAAGCTTCAGATAGtt                                                                                                                  <  2:1986371/148‑1 (MQ=255)
                               tGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGttt                                                                                                                 <  2:2415145/148‑1 (MQ=255)
                               tGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAAGACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGttt                                                                                                                 <  2:2072816/148‑1 (MQ=255)
                                gcgcCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGtttt                                                                                                                <  1:1996098/148‑1 (MQ=255)
                                    cAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAg                                                                                                            <  2:1400844/148‑1 (MQ=255)
                                    cAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAg                                                                                                            <  2:1789618/148‑1 (MQ=255)
                                    cAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAg                                                                                                            <  2:1080986/148‑1 (MQ=255)
                                     aaTCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGt                                                                                                           <  2:2012852/148‑1 (MQ=255)
                                       tCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTc                                                                                                         <  2:1188680/148‑1 (MQ=255)
                                        cACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTcc                                                                                                        <  2:2268327/148‑1 (MQ=255)
                                        cACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTcc                                                                                                        <  2:2205470/148‑1 (MQ=255)
                                          cTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCtg                                                                                                      <  1:2989995/148‑1 (MQ=255)
                                             ccGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGa                                                                                                   <  2:1764816/148‑1 (MQ=255)
                                             ccGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGa                                                                                                   >  1:1981080/1‑148 (MQ=255)
                                                gACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAg                                                                                                >  1:3134636/1‑148 (MQ=255)
                                                gACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAg                                                                                                >  1:1048999/1‑148 (MQ=255)
                                                  cccTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGaa                                                                                              <  2:2320397/148‑1 (MQ=255)
                                                  cccTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGaa                                                                                              <  1:1333040/148‑1 (MQ=255)
                                                            cGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAAt                                                                                    >  2:1401826/1‑148 (MQ=255)
                                                               cACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAg                                                                                 <  2:1584525/148‑1 (MQ=255)
                                                                aCCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGc                                                                                <  1:3422700/148‑1 (MQ=255)
                                                                  cATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCgg                                                                              >  2:3227156/1‑148 (MQ=255)
                                                                   aTGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGt                                                                             >  1:775098/1‑148 (MQ=255)
                                                                    tGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGtt                                                                            >  2:1544313/1‑148 (MQ=255)
                                                                        aaaTACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACg                                                                        <  1:995342/148‑1 (MQ=255)
                                                                         aaTACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGc                                                                       <  2:1459075/148‑1 (MQ=255)
                                                                              gggTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTcca                                                                  <  1:419778/148‑1 (MQ=255)
                                                                              gggTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTcca                                                                  >  2:3116789/1‑148 (MQ=255)
                                                                               ggTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTccac                                                                 <  1:1618492/148‑1 (MQ=255)
                                                                                  aTTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAg                                                              >  1:1424092/1‑148 (MQ=255)
                                                                                   ttCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGt                                                             >  2:3184542/1‑148 (MQ=255)
                                                                                    tCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTg                                                            >  2:679063/1‑148 (MQ=255)
                                                                                         ccGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGaa                                                       <  2:276242/148‑1 (MQ=255)
                                                                                            aTAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAg                                                    >  1:122994/1‑148 (MQ=255)
                                                                                             tAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGATTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTACACCAGTGAGGAATAGc                                                   >  2:2796600/1‑148 (MQ=255)
                                                                                              aacaacTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCg                                                  >  2:2131003/1‑148 (MQ=255)
                                                                                                 aacTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggt                                               >  1:3508378/1‑148 (MQ=255)
                                                                                                   cTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtgg                                             <  2:781100/148‑1 (MQ=255)
                                                                                                   cTTCACCGCCAGTTTCATCAATACTGACCTGCTCTGCAATCTGAATTTGTTTCTCTTTCACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtgg                                             <  2:1849545/148‑1 (MQ=255)
                                                                                                    ttCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtggg                                            >  1:36788/1‑148 (MQ=255)
                                                                                                    ttCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtggg                                            >  1:2494146/1‑148 (MQ=255)
                                                                                                    ttCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtggg                                            >  1:1408372/1‑148 (MQ=255)
                                                                                                       aCCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCgggtggg                                            <  1:2925709/145‑1 (MQ=255)
                                                                                                        ccTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTg                                        <  2:1635058/148‑1 (MQ=255)
                                                                                                           gCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTcg                                     >  1:3386220/1‑148 (MQ=255)
                                                                                                           gCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTcg                                     >  1:375907/1‑148 (MQ=255)
                                                                                                             aGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTcgcg                                   <  2:42069/148‑1 (MQ=255)
                                                                                                              gTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCgcgc                                  >  1:2179633/1‑148 (MQ=255)
                                                                                                                ttCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTg                                >  2:2922912/1‑148 (MQ=255)
                                                                                                                    tcaATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGt                            >  2:3382580/1‑148 (MQ=255)
                                                                                                                    tcaATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGt                            <  2:2606019/148‑1 (MQ=255)
                                                                                                                     caATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGtt                           >  1:3218728/1‑148 (MQ=255)
                                                                                                                     caATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGtt                           <  2:2882001/148‑1 (MQ=255)
                                                                                                                      aataatTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTc                          >  1:2741213/1‑148 (MQ=255)
                                                                                                                       ataatTACCTGCTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCTGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTcg                         <  1:1906630/148‑1 (MQ=255)
                                                                                                                        taatTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTcgc                        >  2:1840857/1‑148 (MQ=255)
                                                                                                                         aatTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGACTAGCGGGTGGGTTTGTCGCGCTGAGGTTcgcg                       >  1:893212/1‑148 (MQ=255)
                                                                                                                           ttACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTACGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGGTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTc                     >  2:1275598/1‑148 (MQ=255)
                                                                                                                           ttACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTc                     <  2:3463642/148‑1 (MQ=255)
                                                                                                                            tACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTcc                    <  1:3184521/148‑1 (MQ=255)
                                                                                                                            tACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTcc                    <  1:3285106/148‑1 (MQ=255)
                                                                                                                             aCCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCa                   >  2:472029/1‑148 (MQ=255)
                                                                                                                              ccTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAg                  >  2:2754440/1‑148 (MQ=255)
                                                                                                                              ccTGGTCTGCAATCTGAATTTTTTTCTATTTAACATTAAGCTTCAGATAGTATTTCAGATCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAg                  >  1:670818/1‑148 (MQ=255)
                                                                                                                                 ggTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTaa               >  2:1897874/1‑148 (MQ=255)
                                                                                                                                  gTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTaaa              <  1:541422/148‑1 (MQ=255)
                                                                                                                                         aTCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCa       <  2:2440904/148‑1 (MQ=255)
                                                                                                                                         aTCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCa       >  1:139825/1‑148 (MQ=255)
                                                                                                                                         aTCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCa       <  2:368838/148‑1 (MQ=255)
                                                                                                                                          tCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCaa      >  2:2094372/1‑148 (MQ=255)
                                                                                                                                           cTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAAt     >  1:2626311/1‑148 (MQ=255)
                                                                                                                                            tGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATa    <  2:1423684/148‑1 (MQ=255)
                                                                                                                                            tGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATa    <  2:1424096/148‑1 (MQ=255)
                                                                                                                                            tGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATa    <  1:472029/148‑1 (MQ=255)
                                                                                                                                             gAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATaa   >  1:1005801/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
AGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAG  >  CP009273/378034‑378323

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTCCA  >  CP009273/378031‑378327
                                                                                                                                                    |                                                                                                                                                    
TATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACGAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTT                                                                                                                                                     >  SRR19973065.2086044/1‑150 (MQ=60)
TATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTT                                                                                                                                                     <  SRR19973065.13442/150‑1 (MQ=60)
TATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTT                                                                                                                                                     <  SRR19973065.1349159/150‑1 (MQ=60)
 ATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTT                                                                                                                                                    >  SRR19973065.642437/1‑150 (MQ=60)
 ATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTT                                                                                                                                                    >  SRR19973065.641387/1‑150 (MQ=60)
  TAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTT                                                                                                                                                   >  SRR19973065.2777178/1‑150 (MQ=60)
  TAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTT                                                                                                                                                   >  SRR19973065.2776684/1‑150 (MQ=60)
    GGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTT                                                                                                                                                 <  SRR19973065.2980499/150‑1 (MQ=60)
    GGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTT                                                                                                                                                 <  SRR19973065.2983123/150‑1 (MQ=60)
    GGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTT                                                                                                                                                 <  SRR19973065.3271026/150‑1 (MQ=60)
     GGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTC                                                                                                                                                >  SRR19973065.1999268/1‑150 (MQ=60)
      GGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCT                                                                                                                                               <  SRR19973065.3530041/150‑1 (MQ=60)
        CAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCT                                                                                                                                             <  SRR19973065.3246999/150‑1 (MQ=60)
        CAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCT                                                                                                                                             <  SRR19973065.3187877/150‑1 (MQ=60)
        CAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCT                                                                                                                                             <  SRR19973065.1808553/150‑1 (MQ=60)
           ATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTA                                                                                                                                          <  SRR19973065.667245/150‑1 (MQ=60)
             CCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAAC                                                                                                                                        >  SRR19973065.2622189/1‑150 (MQ=60)
             CCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAAC                                                                                                                                        >  SRR19973065.1858118/1‑150 (MQ=60)
              CAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACA                                                                                                                                       <  SRR19973065.1911623/150‑1 (MQ=60)
                ATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCTATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATT                                                                                                                                     <  SRR19973065.3093227/150‑1 (MQ=60)
                ATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATT                                                                                                                                     <  SRR19973065.3093214/150‑1 (MQ=60)
                ATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATT                                                                                                                                     >  SRR19973065.1393889/1‑150 (MQ=60)
                  TACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAA                                                                                                                                   >  SRR19973065.1828956/1‑150 (MQ=60)
                  TACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACATGCAGTTTCATCAATAATTACCTGGTCTGCAATATGAATTTTTTTCTCTTTAACATTAA                                                                                                                                   >  SRR19973065.1828945/1‑150 (MQ=60)
                    CCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGC                                                                                                                                 >  SRR19973065.1350933/1‑150 (MQ=60)
                         CAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAG                                                                                                                            <  SRR19973065.2443244/150‑1 (MQ=60)
                         CAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAG                                                                                                                            <  SRR19973065.3498257/150‑1 (MQ=60)
                             AAGTTTGCGACAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCAGATAACAAATTCTCCTGCAGTTTCATAAATAATTACCTGGTATGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAG                                                                                                                        <  SRR19973065.3296622/150‑1 (MQ=60)
                                 TTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTT                                                                                                                    <  SRR19973065.954712/150‑1 (MQ=60)
                                 TTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTT                                                                                                                    <  SRR19973065.759810/150‑1 (MQ=60)
                                 TTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTT                                                                                                                    <  SRR19973065.1181357/150‑1 (MQ=60)
                                   GCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTC                                                                                                                  <  SRR19973065.2009030/150‑1 (MQ=60)
                                   GCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTC                                                                                                                  <  SRR19973065.3345530/150‑1 (MQ=60)
                                    CGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCA                                                                                                                 >  SRR19973065.1412847/1‑150 (MQ=60)
                                         ATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCC                                                                                                            <  SRR19973065.2809456/150‑1 (MQ=60)
                                             CTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTG                                                                                                        <  SRR19973065.3008299/150‑1 (MQ=60)
                                              TACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGA                                                                                                       >  SRR19973065.1993963/1‑150 (MQ=60)
                                                 CGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAG                                                                                                    >  SRR19973065.3153688/1‑150 (MQ=60)
                                                 CGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAG                                                                                                    >  SRR19973065.1055943/1‑150 (MQ=60)
                                                 CGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAG                                                                                                    >  SRR19973065.3153704/1‑150 (MQ=60)
                                                     CCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGG                                                                                                <  SRR19973065.1341863/150‑1 (MQ=60)
                                                     CCCTGAATAACGGCACCATGACAAATACGGGTAGTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGG                                                                                                <  SRR19973065.2147331/150‑1 (MQ=60)
                                                                   ACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGG                                                                                  <  SRR19973065.3443600/150‑1 (MQ=60)
                                                                    CCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGT                                                                                 >  SRR19973065.780248/1‑150 (MQ=60)
                                                                    CCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGT                                                                                 >  SRR19973065.1466557/1‑150 (MQ=60)
                                                                          CAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGC                                                                           >  SRR19973065.795555/1‑150 (MQ=60)
                                                                           AAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCT                                                                          <  SRR19973065.1001958/150‑1 (MQ=60)
                                                                               ACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCA                                                                      <  SRR19973065.2165808/150‑1 (MQ=60)
                                                                                 GGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACC                                                                    <  SRR19973065.422707/150‑1 (MQ=60)
                                                                                 GGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACC                                                                    <  SRR19973065.422702/150‑1 (MQ=60)
                                                                                  GGTTTTCGCGCCCATAACAACTTCAGCTGCAGTTACATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGTCGCTTCCACCA                                                                   <  SRR19973065.3379356/150‑1 (MQ=60)
                                                                                  GGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCA                                                                   <  SRR19973065.1629350/150‑1 (MQ=60)
                                                                                   GTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAG                                                                  >  SRR19973065.1433379/1‑150 (MQ=60)
                                                                                   GTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAG                                                                  >  SRR19973065.1433375/1‑150 (MQ=60)
                                                                                   GTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAG                                                                  >  SRR19973065.1432963/1‑150 (MQ=60)
                                                                                    TATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGT                                                                 >  SRR19973065.1348119/1‑150 (MQ=60)
                                                                                     ATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTG                                                                >  SRR19973065.1974173/1‑150 (MQ=60)
                                                                                             CGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAG                                                        >  SRR19973065.124065/1‑150 (MQ=60)
                                                                                              GATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCATTGCGGAATAGC                                                       >  SRR19973065.1824570/1‑150 (MQ=60)
                                                                                              GATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGC                                                       >  SRR19973065.2955570/1‑150 (MQ=60)
                                                                                                  ACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGT                                                   >  SRR19973065.3529667/1‑150 (MQ=60)
                                                                                                     ACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGG                                                >  SRR19973065.37241/1‑150 (MQ=60)
                                                                                                     ACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGG                                                >  SRR19973065.1912816/1‑150 (MQ=60)
                                                                                                     ACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGG                                                >  SRR19973065.1417588/1‑150 (MQ=60)
                                                                                                     ACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGG                                                >  SRR19973065.2509839/1‑150 (MQ=60)
                                                                                                     ACTTCACCTGCAGTTTCATCAATAAATACCTGGACTCCAAACTGAATTTTTTTCTCTTAAACATAAACATACAGATAGTTTTTCAGTTCCTGTGACAGAATGTAGGGAATAAGAGGATCACGCTACCACCAGTGCGGAATAGCGCGTGGG                                                >  SRR19973065.2509834/1‑150 (MQ=60)
                                                                                                      CTTCACCTGCAGTGTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGT                                               <  SRR19973065.2990239/150‑1 (MQ=60)
                                                                                                       TTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTT                                              <  SRR19973065.2943483/150‑1 (MQ=60)
                                                                                                            CTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCG                                         >  SRR19973065.378529/1‑150 (MQ=60)
                                                                                                            CTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCG                                         >  SRR19973065.378287/1‑150 (MQ=60)
                                                                                                            CTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCG                                         <  SRR19973065.2643728/150‑1 (MQ=60)
                                                                                                            CTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCG                                         >  SRR19973065.3406928/1‑150 (MQ=60)
                                                                                                               CAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGC                                      >  SRR19973065.2193612/1‑150 (MQ=60)
                                                                                                                AGTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCT                                     <  SRR19973065.444632/150‑1 (MQ=60)
                                                                                                                 GTTTCATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTG                                    >  SRR19973065.341569/1‑150 (MQ=60)
                                                                                                                      ATCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTT                               >  SRR19973065.3238079/1‑150 (MQ=60)
                                                                                                                       TCAATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTC                              >  SRR19973065.2758260/1‑150 (MQ=60)
                                                                                                                          ATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGACTAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCG                           >  SRR19973065.898886/1‑150 (MQ=60)
                                                                                                                          ATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCG                           >  SRR19973065.1308400/1‑150 (MQ=60)
                                                                                                                          ATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCG                           >  SRR19973065.1040078/1‑150 (MQ=60)
                                                                                                                          ATAATTACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCG                           >  SRR19973065.612426/1‑150 (MQ=60)
                                                                                                                          ATAATTACCTGCTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCTGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCG                           <  SRR19973065.1919156/150‑1 (MQ=60)
                                                                                                                               TACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAG                      <  SRR19973065.3203750/150‑1 (MQ=60)
                                                                                                                               TACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAG                      <  SRR19973065.3305081/150‑1 (MQ=60)
                                                                                                                               TACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAG                      <  SRR19973065.3203771/150‑1 (MQ=60)
                                                                                                                               TACCTGGTCTGCAATCTGAATTTTTTTCTATTTAACATTAAGCTTCAGATAGTATTTCAGATCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAG                      >  SRR19973065.675290/1‑150 (MQ=60)
                                                                                                                                ACCTGGTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGT                     <  SRR19973065.477907/150‑1 (MQ=60)
                                                                                                                                     GTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAA                <  SRR19973065.544935/150‑1 (MQ=60)
                                                                                                                                     GTCTGCAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAA                <  SRR19973065.2320855/150‑1 (MQ=60)
                                                                                                                                          CAATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCA           >  SRR19973065.141021/1‑150 (MQ=60)
                                                                                                                                           AATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAA          >  SRR19973065.711070/1‑150 (MQ=60)
                                                                                                                                           AATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAA          >  SRR19973065.776480/1‑150 (MQ=60)
                                                                                                                                            ATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAAT         >  SRR19973065.1879591/1‑150 (MQ=60)
                                                                                                                                            ATCTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAAT         >  SRR19973065.2642558/1‑150 (MQ=60)
                                                                                                                                              CTGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAA       >  SRR19973065.1012479/1‑150 (MQ=60)
                                                                                                                                               TGAATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAG      <  SRR19973065.475260/150‑1 (MQ=60)
                                                                                                                                                 AATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTC    <  SRR19973065.1764637/150‑1 (MQ=60)
                                                                                                                                                  ATTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTCC   <  SRR19973065.64046/150‑1 (MQ=60)
                                                                                                                                                   TTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTCCA  <  SRR19973065.1179136/150‑1 (MQ=60)
                                                                                                                                                   TTTTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTCCA  <  SRR19973065.2723879/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TATAGGGGCAAATCCAATTACCTATCAGGCAGTTTGCGCCAATCACTACCGGACCCTGAATAACGGCACCATGACAAATACGGGTATTCGCGCCGATAACAACTTCACCTGCAGTTTCATCAATAATTACCTGGTCTGCAATCTGAATATTTTTCTCTTTAACATTAAGCTTCAGATAGTTTTTCAGTTCCTGTGACAGAAGGTTGGGAATAAGCGGTTGACGCTTCCACCAGTGCGGAATAGCGGGTGGGTTTGTCGCGCTGAGGTTCGCGTCCAGTAAAAAAGGCAATAAGTCCA  >  CP009273/378031‑378327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑