Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,755 C→A 100% D45Y (GAC→TAC)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,7550CA99.1% 729.6 / ‑2.0 226D45Y (GAC→TAC) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (108/115);  total (109/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

TTTTACCACTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCA  >  CP009273/2711611‑2711898
                                                                                                                                                  |                                                                                                                                               
     ccACTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgag                                                                                                                                         >  2:943953/1‑148 (MQ=255)
     ccACTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgag                                                                                                                                         >  2:1340080/1‑148 (MQ=255)
      cACTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGACAGCACgaga                                                                                                                                        >  2:284285/1‑148 (MQ=255)
        cTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                      >  2:1284496/1‑148 (MQ=255)
        cTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                      >  1:3001757/1‑148 (MQ=255)
        cTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                      >  1:455936/1‑148 (MQ=255)
          gATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtttt                                                                                                                                    >  1:1599463/1‑148 (MQ=255)
          gATGCCACCGTGATCGACCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtttt                                                                                                                                    >  2:2051879/1‑148 (MQ=255)
           aTGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTc                                                                                                                                   >  1:2244933/1‑148 (MQ=255)
             gCCCCCGTGCTCGGCCTTGTCCCGCCGACACCCCCCCCGGCGCGCGGCAACCCGCC‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTcc                                                                                                                                  <  1:2654456/148‑1 (MQ=255)
             gCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                                 >  2:1097354/1‑148 (MQ=255)
              ccACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCATCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTg                                                                                                                                >  1:1513960/1‑148 (MQ=255)
                    tGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgcc                                                                                                                          >  1:2083733/1‑148 (MQ=255)
                    tGATCGGCCTT‑TGCCGGCGTCGCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgcc                                                                                                                          <  2:2260399/148‑1 (MQ=255)
                          gCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGcccc                                                                                                                    >  1:1584323/1‑148 (MQ=255)
                          cccTT‑GTCCGGCGCCACACCCCCGGGCGCGCGGCACCCGGGC‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGcccc                                                                                                                    <  1:58593/147‑1 (MQ=255)
                           ccTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                   >  2:498200/1‑148 (MQ=255)
                             tt‑ttCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCtt                                                                                                                 >  1:3429052/1‑148 (MQ=255)
                                      cGTCACACCCCCGGGCGCGCGGCAACCCGGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTa                                                                                                         <  1:318185/148‑1 (MQ=255)
                                        tctcATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcg                                                                                                       >  1:432823/1‑148 (MQ=255)
                                         ctcATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgc                                                                                                      >  1:2008934/1‑148 (MQ=255)
                                          tcatcaCCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAcgcg                                                                                                     >  2:3467273/1‑148 (MQ=255)
                                            atccCCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCggg                                                                                                   <  2:3129531/148‑1 (MQ=255)
                                             tcaCCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                                                                                                  >  1:3499507/1‑148 (MQ=255)
                                               ccccGGCGCGCGGTAAACCGGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                                                                                                <  1:2331048/147‑1 (MQ=255)
                                               ccccGGCGCGC‑GCAAACCGGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtg                                                                                                <  1:2769413/147‑1 (MQ=255)
                                                ccgggCGCGCGGCAACGGGGG‑CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                                                                                               <  1:859134/145‑1 (MQ=255)
                                                cccGGCGCGCGGCAAACGGGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                                                                                               <  1:859105/148‑1 (MQ=255)
                                                     cgcgcgGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTa                                                                                          >  1:690645/1‑148 (MQ=255)
                                                         cgGGAAACGGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGtt                                                                                      <  2:2714693/148‑1 (MQ=255)
                                                            gAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                   >  1:574430/1‑148 (MQ=255)
                                                            gAAACCGTG‑CGCCACGGAGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                   <  2:1395154/148‑1 (MQ=255)
                                                            caacccGGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAt                                                                                   <  2:1314199/144‑1 (MQ=255)
                                                             aacccGGG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc                                                                                  <  2:2785988/145‑1 (MQ=255)
                                                             aaaCCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTCCGTTTATc                                                                                  >  2:2124949/1‑148 (MQ=255)
                                                                ccGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                               >  1:1496946/1‑148 (MQ=255)
                                                                 ggTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCTCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                              <  2:3141058/147‑1 (MQ=255)
                                                                  gggcccccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                              <  1:2122530/142‑1 (MQ=255)
                                                                  cgggccccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                              <  1:2156812/142‑1 (MQ=255)
                                                                   tG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatc                                                                            <  2:3001757/148‑1 (MQ=255)
                                                                   gg‑cgcCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatc                                                                            <  2:2656926/147‑1 (MQ=255)
                                                                    gcccccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATAAGAatc                                                                            <  1:3335178/144‑1 (MQ=255)
                                                                    g‑cgcCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatca                                                                           <  2:3047192/148‑1 (MQ=255)
                                                                      cgcCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcat                                                                          <  2:3146614/148‑1 (MQ=255)
                                                                        ccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatca                                                                        <  2:379654/148‑1 (MQ=255)
                                                                         cACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatcatcat                                                                       <  1:2623835/148‑1 (MQ=255)
                                                                           cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTGTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                     <  2:2235641/148‑1 (MQ=255)
                                                                              cGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTg                                                                  <  2:2461293/148‑1 (MQ=255)
                                                                              cGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTg                                                                  >  1:2429117/1‑148 (MQ=255)
                                                                                aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCg                                                                >  2:1770242/1‑148 (MQ=255)
                                                                                aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTGATCAGAATCATCATCACTGCg                                                                >  2:2884769/1‑148 (MQ=255)
                                                                                 aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                               >  1:1719970/1‑148 (MQ=255)
                                                                                 aTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                               >  1:2709627/1‑148 (MQ=255)
                                                                                 aTCGTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCgg                                                               <  2:2721443/148‑1 (MQ=255)
                                                                                  tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                              >  1:1176587/1‑148 (MQ=255)
                                                                                  tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                              >  1:12002/1‑148 (MQ=255)
                                                                                  tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                              >  2:2889902/1‑148 (MQ=255)
                                                                                  tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                              <  1:2868710/148‑1 (MQ=255)
                                                                                  tCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGa                                                              >  1:975169/1‑148 (MQ=255)
                                                                                   cTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac                                                             <  2:3428206/148‑1 (MQ=255)
                                                                                   cTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGACCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGac                                                             >  2:1230043/1‑148 (MQ=255)
                                                                                    ttCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGaca                                                            >  1:1815044/1‑148 (MQ=255)
                                                                                          cTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATc                                                      >  2:1819334/1‑148 (MQ=255)
                                                                                           ttGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCa                                                     <  1:1110353/148‑1 (MQ=255)
                                                                                           ttGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCa                                                     <  1:3065305/148‑1 (MQ=255)
                                                                                            tGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCAc                                                    <  2:3499507/148‑1 (MQ=255)
                                                                                             gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                                                   <  1:3215663/148‑1 (MQ=255)
                                                                                             gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                                                   <  1:1670193/148‑1 (MQ=255)
                                                                                              cGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACtt                                                  <  2:585115/148‑1 (MQ=255)
                                                                                              cGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACtt                                                  <  2:2885994/148‑1 (MQ=255)
                                                                                                gTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTg                                                <  1:1109122/148‑1 (MQ=255)
                                                                                                 tCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGa                                               >  2:1616117/1‑148 (MQ=255)
                                                                                                   aCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAcc                                             <  1:3136824/148‑1 (MQ=255)
                                                                                                         cTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCg                                       >  2:594804/1‑148 (MQ=255)
                                                                                                          tGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGc                                      <  2:2307047/148‑1 (MQ=255)
                                                                                                          tGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGGAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGc                                      <  2:2637083/148‑1 (MQ=255)
                                                                                                            aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                    >  1:2481888/1‑148 (MQ=255)
                                                                                                            aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                    >  2:2117275/1‑148 (MQ=255)
                                                                                                             ctctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt                                   <  2:1513960/148‑1 (MQ=255)
                                                                                                              tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                  >  2:2002458/1‑148 (MQ=255)
                                                                                                              tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                  <  1:2916611/148‑1 (MQ=255)
                                                                                                              tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                  <  2:752094/148‑1 (MQ=255)
                                                                                                                     gTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttc                           <  1:1340080/148‑1 (MQ=255)
                                                                                                                       cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtgtcat                         >  2:2571533/1‑148 (MQ=255)
                                                                                                                            cgggcgggGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTttcatttcat                    <  1:3206669/148‑1 (MQ=255)
                                                                                                                              ggcggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCg                  <  1:938125/148‑1 (MQ=255)
                                                                                                                                cggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGtt                <  2:3376818/148‑1 (MQ=255)
                                                                                                                                 ggggcgACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTc               <  2:2523268/148‑1 (MQ=255)
                                                                                                                                 ggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTc               <  2:1974340/148‑1 (MQ=255)
                                                                                                                                  gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa              >  2:2181541/1‑148 (MQ=255)
                                                                                                                                  gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa              <  2:932393/148‑1 (MQ=255)
                                                                                                                                   ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCAt             >  2:2696989/1‑148 (MQ=255)
                                                                                                                                   ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTGTATCAGAATCATCATCACTGCGGACATACATCACGTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCAt             >  2:2056229/1‑148 (MQ=255)
                                                                                                                                    gcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATg            >  2:1728230/1‑148 (MQ=255)
                                                                                                                                    gcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATg            <  1:1788661/148‑1 (MQ=255)
                                                                                                                                     cgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGc           <  2:257962/148‑1 (MQ=255)
                                                                                                                                      gACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCt          <  1:2879993/148‑1 (MQ=255)
                                                                                                                                       aCGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtt         <  1:3408742/148‑1 (MQ=255)
                                                                                                                                         ggCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtttt       <  2:3269147/148‑1 (MQ=255)
                                                                                                                                            cACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTtctc    <  2:889084/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
TTTTACCACTGATGCCACCGTGATCGGCCTT‑TTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTG‑CGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCA  >  CP009273/2711611‑2711898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCG  >  CP009273/2711609‑2711904
                                                                                                                                                   |                                                                                                                                                     
ACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGC                                                                                                                                                    >  SRR19973065.1668905/1‑150 (MQ=60)
    TTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGA                                                                                                                                                >  SRR19973065.139475/1‑150 (MQ=60)
      cccccctgcggccccggtgagcggcttgtcccggcgacccacccccgggcgggggcacacccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAG                                                                                                                                               <  SRR19973065.1020712/83‑1 (MQ=60)
      ACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGA                                                                                                                                              >  SRR19973065.1514463/1‑150 (MQ=60)
        CACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATT                                                                                                                                            >  SRR19973065.3020148/1‑150 (MQ=60)
        CACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATT                                                                                                                                            >  SRR19973065.459054/1‑150 (MQ=60)
        CACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATT                                                                                                                                            >  SRR19973065.459086/1‑150 (MQ=60)
         ACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTT                                                                                                                                           >  SRR19973065.634774/1‑150 (MQ=60)
          CTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTT                                                                                                                                          >  SRR19973065.1610146/1‑150 (MQ=60)
          CTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTT                                                                                                                                          >  SRR19973065.2788581/1‑150 (MQ=60)
           cggcgccccgcgtgcggggggttggcgggcgacacccccccgggcgcgcgccaccccgccccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTT                                                                                                                                          <  SRR19973065.622278/88‑1 (MQ=60)
           TGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTC                                                                                                                                         >  SRR19973065.2259187/1‑150 (MQ=60)
            gagccccCCGTGAGCGGCCTTTGCCGGCGTCACACCCCCCGGCGGGGGGGAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCC                                                                                                                                        <  SRR19973065.2894689/143‑1 (MQ=60)
            GATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCC                                                                                                                                        >  SRR19973065.1804828/1‑150 (MQ=60)
             ATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCCCCCGGCGGGCGGCAACCC‑GGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCT                                                                                                                                       <  SRR19973065.1758208/150‑1 (MQ=60)
              TGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCATCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTG                                                                                                                                      >  SRR19973065.1523844/1‑150 (MQ=60)
               ggcgcggtttatctgctttttcggggttcactccccccggggggctgaacagggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTG                                                                                                                                      <  SRR19973065.1326544/92‑1 (MQ=60)
               gcccccgtgctcggccttgtcccgccgacaccccccccggcgcgcggcaacccgccccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTG                                                                                                                                      <  SRR19973065.2670854/92‑1 (MQ=60)
               gcccccggtgcccggggtgtcccggcgacccaccccccggcgcgcgcaaacccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTG                                                                                                                                      <  SRR19973065.2671031/92‑1 (MQ=60)
               GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGG                                                                                                                                     >  SRR19973065.2929585/1‑150 (MQ=60)
                  ACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACG                                                                                                                                  >  SRR19973065.2901053/1‑150 (MQ=60)
                  ACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACG                                                                                                                                  >  SRR19973065.1867044/1‑150 (MQ=60)
                    CGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCC                                                                                                                                >  SRR19973065.2097092/1‑150 (MQ=60)
                       tgtggggcctgtcgcgccgcccccccccggggggcggggccccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCG                                                                                                                              <  SRR19973065.4670/100‑1 (MQ=60)
                       tgaggggctttgccgggcgtcccccccccgggggggcgccaccccgccccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCG                                                                                                                              <  SRR19973065.4674/100‑1 (MQ=60)
                       GATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGC                                                                                                                             >  SRR19973065.1188350/1‑150 (MQ=60)
                          CGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCC                                                                                                                          >  SRR19973065.1594888/1‑150 (MQ=60)
                          CGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCC                                                                                                                          >  SRR19973065.1593910/1‑150 (MQ=60)
                           GGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCC                                                                                                                         >  SRR19973065.2571496/1‑150 (MQ=60)
                             cgttggcgggggtaacacccccgGGCGCGCGGGAACCG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       <  SRR19973065.2254584/127‑1 (MQ=60)
                             cgttgccgGGCGTCTCACCCCCCGGCGGGCGGCAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       <  SRR19973065.2253452/142‑1 (MQ=60)
                             cccttgtccggcgccacacccccgggcgcgcggcacccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCT                                                                                                                        <  SRR19973065.59219/106‑1 (MQ=60)
                             CCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       >  SRR19973065.3449125/1‑150 (MQ=60)
                             CCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       >  SRR19973065.160464/1‑150 (MQ=60)
                             CCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       >  SRR19973065.3449974/1‑150 (MQ=60)
                             CCTTGGCCGGCGTCTCAACCCCCGGCGCGCGGGACCCC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       <  SRR19973065.203065/150‑1 (MQ=60)
                              cctttgcgggcgtctcaccccccgggggggggcaaacgggGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTT                                                                                                                       <  SRR19973065.2253427/110‑1 (MQ=60)
                               ttttgcgggcgccacacccccgggcgcgGGGAAACCGGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTT                                                                                                                      <  SRR19973065.834965/122‑1 (MQ=60)
                                       gggtctcacccccgggggcggggaacccggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAC                                                                                                              <  SRR19973065.3274548/116‑1 (MQ=60)
                                       gggtcacaccccccggcgcgcggtaccccgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTAC                                                                                                              <  SRR19973065.142510/116‑1 (MQ=60)
                                       CGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACG                                                                                                             >  SRR19973065.435831/1‑150 (MQ=60)
                                       CGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACG                                                                                                             >  SRR19973065.435971/1‑150 (MQ=60)
                                        cgtcacacccccgggcgcgcggcaacccgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACG                                                                                                             <  SRR19973065.320372/117‑1 (MQ=60)
                                        GTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGC                                                                                                            >  SRR19973065.237242/1‑150 (MQ=60)
                                        GTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGC                                                                                                            >  SRR19973065.1722554/1‑150 (MQ=60)
                                        GTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGC                                                                                                            >  SRR19973065.2021902/1‑150 (MQ=60)
                                         aatcacccCCCGGCGCGCGGGAAACG‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCG                                                                                                           <  SRR19973065.845472/142‑1 (MQ=60)
                                          CTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGG                                                                                                          >  SRR19973065.2479392/1‑150 (MQ=60)
                                            CATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                        >  SRR19973065.3520765/1‑150 (MQ=60)
                                               cccccgggcgggcggcaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTG                                                                                                      <  SRR19973065.676217/124‑1 (MQ=60)
                                                ccccgggcgcgcgccaaccgggcCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGT                                                                                                     <  SRR19973065.321018/127‑1 (MQ=60)
                                                cccccggcgcggggcaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGT                                                                                                     <  SRR19973065.320653/125‑1 (MQ=60)
                                                CCCCGGCGCGCGGTAAACC‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTG                                                                                                    <  SRR19973065.2345625/150‑1 (MQ=60)
                                                 ccccggcgcgcggcaccccggcccCCACGGCGAATCGTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTG                                                                                                    <  SRR19973065.777785/126‑1 (MQ=60)
                                                 ccccggcgcgcgcaaaccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTG                                                                                                    <  SRR19973065.2786616/126‑1 (MQ=60)
                                                 CCCGGCGCGCGGCAAACG‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.864644/150‑1 (MQ=60)
                                                  ccggggggggggcaccccgtcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.2632810/127‑1 (MQ=60)
                                                  ccgggcgcggggcaacccgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.864645/127‑1 (MQ=60)
                                                  ccgggcgcgcggcaacgggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.864673/127‑1 (MQ=60)
                                                  ccgggcgcgcggcaacgcgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.3480910/127‑1 (MQ=60)
                                                  ccgggcgcgcggcaaccgggGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                   <  SRR19973065.864534/130‑1 (MQ=60)
                                                   gcggcgggcgccaccccgccccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGG                                                                                                  <  SRR19973065.318939/128‑1 (MQ=60)
                                                   ccggggcgggcccccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGG                                                                                                  <  SRR19973065.318962/128‑1 (MQ=60)
                                                    GGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTA                                                                                                >  SRR19973065.2466980/1‑150 (MQ=60)
                                                    GGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTA                                                                                                >  SRR19973065.695244/1‑150 (MQ=60)
                                                           GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAT                                                                                         >  SRR19973065.577992/1‑150 (MQ=60)
                                                           GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAT                                                                                         >  SRR19973065.578044/1‑150 (MQ=60)
                                                           GGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAT                                                                                         >  SRR19973065.578094/1‑150 (MQ=60)
                                                             gcaccgcgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATC                                                                                        <  SRR19973065.3292293/138‑1 (MQ=60)
                                                             gcaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATC                                                                                        <  SRR19973065.3293027/138‑1 (MQ=60)
                                                               AACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGA                                                                                     >  SRR19973065.1506581/1‑150 (MQ=60)
                                                                  C‑GGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATC                                                                                  <  SRR19973065.2137235/150‑1 (MQ=60)
                                                                  C‑GGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATC                                                                                  <  SRR19973065.2170680/150‑1 (MQ=60)
                                                                   gggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATC                                                                                  <  SRR19973065.2137360/144‑1 (MQ=60)
                                                                   gggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATC                                                                                  <  SRR19973065.2136197/144‑1 (MQ=60)
                                                                     ggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATAAGAATCAT                                                                                <  SRR19973065.3355416/146‑1 (MQ=60)
                                                                     gcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATAAGAATCAT                                                                                <  SRR19973065.3355537/146‑1 (MQ=60)
                                                                        GCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCAT                                                                             <  SRR19973065.3163567/150‑1 (MQ=60)
                                                                         CCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATC                                                                            <  SRR19973065.3485970/150‑1 (MQ=60)
                                                                          CACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCA                                                                           <  SRR19973065.2640071/150‑1 (MQ=60)
                                                                           ACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCAC                                                                          <  SRR19973065.3145003/150‑1 (MQ=60)
                                                                             GGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTG                                                                        >  SRR19973065.2444447/1‑150 (MQ=60)
                                                                             GGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTG                                                                        >  SRR19973065.2655358/1‑150 (MQ=60)
                                                                                GAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGG                                                                     >  SRR19973065.2726476/1‑150 (MQ=60)
                                                                                GAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGG                                                                     >  SRR19973065.1731396/1‑150 (MQ=60)
                                                                                GAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGG                                                                     >  SRR19973065.892559/1‑150 (MQ=60)
                                                                                GAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGG                                                                     >  SRR19973065.2726497/1‑150 (MQ=60)
                                                                                 AATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGA                                                                    >  SRR19973065.12179/1‑150 (MQ=60)
                                                                                 AATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGA                                                                    >  SRR19973065.1184063/1‑150 (MQ=60)
                                                                                 AATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGA                                                                    >  SRR19973065.981647/1‑150 (MQ=60)
                                                                                  ATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGAC                                                                   >  SRR19973065.3366767/1‑150 (MQ=60)
                                                                                   TCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACA                                                                  >  SRR19973065.1823356/1‑150 (MQ=60)
                                                                                   TCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACA                                                                  >  SRR19973065.1826994/1‑150 (MQ=60)
                                                                                   TCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACA                                                                  <  SRR19973065.2886305/150‑1 (MQ=60)
                                                                                            TTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACT                                                         <  SRR19973065.3084047/150‑1 (MQ=60)
                                                                                            TTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACT                                                         <  SRR19973065.1117611/150‑1 (MQ=60)
                                                                                            TTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACT                                                         <  SRR19973065.1117616/150‑1 (MQ=60)
                                                                                              GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                       <  SRR19973065.2758728/150‑1 (MQ=60)
                                                                                              GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                       <  SRR19973065.3235002/150‑1 (MQ=60)
                                                                                              GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                       <  SRR19973065.1681366/150‑1 (MQ=60)
                                                                                              GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                       <  SRR19973065.3192473/150‑1 (MQ=60)
                                                                                                 GTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAC                                                    <  SRR19973065.1116380/150‑1 (MQ=60)
                                                                                                    ACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTT                                                 <  SRR19973065.3155902/150‑1 (MQ=60)
                                                                                                           TGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTT                                          >  SRR19973065.2286708/1‑150 (MQ=60)
                                                                                                           TGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTT                                          >  SRR19973065.2497509/1‑150 (MQ=60)
                                                                                                            GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCgtg                                         >  SRR19973065.1139083/1‑147 (MQ=60)
                                                                                                            GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTGT                                         >  SRR19973065.1248044/1‑150 (MQ=60)
                                                                                                             ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                        <  SRR19973065.2099273/150‑1 (MQ=60)
                                                                                                             ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                        >  SRR19973065.2955564/1‑150 (MQ=60)
                                                                                                               TCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAC                                      <  SRR19973065.2934352/150‑1 (MQ=60)
                                                                                                                 TGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCT                                    <  SRR19973065.3408176/150‑1 (MQ=60)
                                                                                                                 TGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCT                                    <  SRR19973065.420461/150‑1 (MQ=60)
                                                                                                                      GTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCAT                               <  SRR19973065.1348950/150‑1 (MQ=60)
                                                                                                                             CGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCG                        <  SRR19973065.2673182/150‑1 (MQ=60)
                                                                                                                             CGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCG                        <  SRR19973065.3225969/150‑1 (MQ=60)
                                                                                                                             CGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCG                        <  SRR19973065.3225983/150‑1 (MQ=60)
                                                                                                                               GGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTT                      <  SRR19973065.3059578/150‑1 (MQ=60)
                                                                                                                               GGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTT                      <  SRR19973065.1303779/150‑1 (MQ=60)
                                                                                                                               GGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTT                      <  SRR19973065.944326/150‑1 (MQ=60)
                                                                                                                                   GGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATG                  <  SRR19973065.1607382/150‑1 (MQ=60)
                                                                                                                                     GCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCT                <  SRR19973065.1800377/150‑1 (MQ=60)
                                                                                                                                      CGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTT               <  SRR19973065.94929/150‑1 (MQ=60)
                                                                                                                                       GACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTT              <  SRR19973065.2897624/150‑1 (MQ=60)
                                                                                                                                        ACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTT             <  SRR19973065.3429570/150‑1 (MQ=60)
                                                                                                                                                CGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCA     >  SRR19973065.3455414/1‑150 (MQ=60)
                                                                                                                                                 GTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAG    <  SRR19973065.3192913/150‑1 (MQ=60)
                                                                                                                                                  TAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGC   <  SRR19973065.2813731/150‑1 (MQ=60)
                                                                                                                                                   AAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTGCCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCG  >  SRR19973065.179336/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
ACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACC‑GTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCAGCG  >  CP009273/2711609‑2711904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑