Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,711,755 C→A 100% D45Y (GAC→TAC)  yfiF ← putative methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,711,7550CA98.1% 652.7 / 2.2 204D45Y (GAC→TAC) yfiFputative methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base A (96/104);  total (100/104)
Fisher's exact test for biased strand distribution p-value = 5.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 204 total aligned reads displayed.

CTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCAC  >  CP009273/2711610‑2711899
                                                                                                                                                 |                                                                                                                                                
 ttttCCCCCTGAGCCCCCGGTGAGCGGCGTTTCCCGGCGACTCAACACCCGGCGCGCGGGAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGc                                                                                                                                               <  2:3286899/148‑1 (MQ=255)
      ccACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGGGGGAAACGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgag                                                                                                                                          <  2:3813365/148‑1 (MQ=255)
      ccACTCAGGCCACCGTGTTCGGCTTTGCCCGGGGTCACACCCCCCGGCGCGCGGAAACCCGTCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACgag                                                                                                                                          <  2:2561484/148‑1 (MQ=255)
         cTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAtt                                                                                                                                       >  1:3083284/1‑148 (MQ=255)
          tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAttt                                                                                                                                      >  1:3726193/1‑148 (MQ=255)
          tGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAttt                                                                                                                                      >  2:2537679/1‑148 (MQ=255)
             tGCCACGGTGAGCGGCCTTTCCCGGCGACACACCCCCCGGCGCGGGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTcc                                                                                                                                   <  1:2826609/148‑1 (MQ=255)
             gccccccGTGAGCGGCCTTGCCCGGCGTCACATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTcc                                                                                                                                   <  2:2478450/143‑1 (MQ=255)
              gCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACACTGTTTGTCATCGCGGGCGGGGCGACGGACACCGTAAGCACGAGATTTTCCt                                                                                                                                  >  2:160964/1‑148 (MQ=255)
              gCCACCGTGACCGGCCTTTCCCGGCGTCTCATCACCCGGCGCGCGGGAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCt                                                                                                                                  <  2:3158175/148‑1 (MQ=255)
                cACCGTGATGGGCCTTGGCCGGCGTCACATCCCCGGGCGCGCGGTAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTgg                                                                                                                                <  1:3317921/148‑1 (MQ=255)
                cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTgg                                                                                                                                >  2:1925733/1‑148 (MQ=255)
                cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTgg                                                                                                                                >  1:1830952/1‑148 (MQ=255)
                cACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTgg                                                                                                                                >  1:2411912/1‑148 (MQ=255)
                 aCCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGa                                                                                                                               >  2:356767/1‑148 (MQ=255)
                    gTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgc                                                                                                                            >  1:26272/1‑148 (MQ=255)
                     tGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgcc                                                                                                                           >  2:1790326/1‑148 (MQ=255)
                      gATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgccc                                                                                                                          >  1:1030633/1‑148 (MQ=255)
                       aTCGGCCTTTGCCGGCTTCTCACCACCCGGCGCGCGGTAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAcgcccg                                                                                                                         <  2:2784726/148‑1 (MQ=255)
                           gCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGcccc                                                                                                                     >  1:1061105/1‑148 (MQ=255)
                            ccttggccGGCGTCACAACACCCGGCGCGGGGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGccccc                                                                                                                    <  2:2614940/142‑1 (MQ=255)
                             ttgggccGGCGCCACACCCCCGGGCGCGCGGCACCCGGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                                                                                                   <  1:162289/143‑1 (MQ=255)
                             cTTTGCCGGCGTCTCACCCCCGGGGGCGCGTCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCt                                                                                                                   <  1:2091943/148‑1 (MQ=255)
                                     gCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGt                                                                                                           >  2:1434756/1‑148 (MQ=255)
                                           catcaCCCGGCGCGCGGTAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCgg                                                                                                     <  2:1161509/148‑1 (MQ=255)
                                             tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                                                                                                   >  1:14864/1‑148 (MQ=255)
                                             tcaCCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGt                                                                                                   >  1:2156721/1‑148 (MQ=255)
                                              caCCCGGCGCGCGGCAACCGGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGtt                                                                                                  <  2:446704/148‑1 (MQ=255)
                                                ccgggggcgcgGGACCCCGGCCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgt                                                                                                <  1:732391/142‑1 (MQ=255)
                                                 ccGGCGCGCGGTAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTtgtg                                                                                               <  1:935404/148‑1 (MQ=255)
                                                    gcgcgcGGGAACCCGGCCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGt                                                                                            <  2:1830898/148‑1 (MQ=255)
                                                       cgcgGGAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACg                                                                                         <  2:2135489/148‑1 (MQ=255)
                                                        gcgGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGt                                                                                        <  1:2132267/148‑1 (MQ=255)
                                                             aaaCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATc                                                                                   >  1:647901/1‑148 (MQ=255)
                                                               aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg                                                                                 >  2:269431/1‑148 (MQ=255)
                                                               aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg                                                                                 >  2:310278/1‑148 (MQ=255)
                                                               aCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAg                                                                                 >  2:733914/1‑148 (MQ=255)
                                                                cggtcccccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                                <  1:1110910/141‑1 (MQ=255)
                                                                ccGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                                >  2:1550854/1‑148 (MQ=255)
                                                                ccGTGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                                <  2:1619351/148‑1 (MQ=255)
                                                                ccGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGa                                                                                <  1:3576687/148‑1 (MQ=255)
                                                                 cGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGaa                                                                               >  2:2871863/1‑148 (MQ=255)
                                                                   cgccccACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAatc                                                                             <  1:18229/144‑1 (MQ=255)
                                                                          cGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTGTGTCAGCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                      <  2:3230103/148‑1 (MQ=255)
                                                                          cGGCGAATCGTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAtcatcatca                                                                      <  2:144321/148‑1 (MQ=255)
                                                                           ggCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCAc                                                                     <  1:2008054/148‑1 (MQ=255)
                                                                             cGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTg                                                                   >  1:3803178/1‑148 (MQ=255)
                                                                               aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCg                                                                 >  1:2936006/1‑148 (MQ=255)
                                                                               aaTCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCg                                                                 >  1:3102197/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  2:515344/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  1:1752814/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  2:65823/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  1:3533118/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  1:600181/1‑148 (MQ=255)
                                                                                    tCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGacat                                                            >  1:2993725/1‑148 (MQ=255)
                                                                                          ttGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCa                                                      <  2:188677/148‑1 (MQ=255)
                                                                                           tGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCAc                                                     <  2:1571238/148‑1 (MQ=255)
                                                                                            gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                                                    >  1:3380726/1‑148 (MQ=255)
                                                                                            gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                                                    <  2:1340072/148‑1 (MQ=255)
                                                                                            gCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACt                                                    <  1:1002941/148‑1 (MQ=255)
                                                                                               gTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTg                                                 >  2:3208683/1‑148 (MQ=255)
                                                                                                  aCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAcc                                              <  2:1375859/148‑1 (MQ=255)
                                                                                                  aCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAcc                                              >  2:3121261/1‑148 (MQ=255)
                                                                                                  aCGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGAcc                                              <  1:1312461/148‑1 (MQ=255)
                                                                                                     gggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTg                                           <  1:1283160/148‑1 (MQ=255)
                                                                                                      ggCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTCCGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGc                                          >  1:1167042/1‑148 (MQ=255)
                                                                                                          gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCt                                      <  1:3042843/148‑1 (MQ=255)
                                                                                                          gACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCt                                      <  1:2729901/148‑1 (MQ=255)
                                                                                                           aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                     >  2:2944154/1‑148 (MQ=255)
                                                                                                           aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                     <  1:1109730/148‑1 (MQ=255)
                                                                                                           aCTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtt                                     <  1:526023/148‑1 (MQ=255)
                                                                                                            ctctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCttt                                    >  1:1814886/1‑148 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                   <  2:55570/148‑1 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                   >  1:3623066/1‑148 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                   <  1:1790326/148‑1 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                   <  1:3683063/148‑1 (MQ=255)
                                                                                                             tctGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCtttt                                   <  2:2446517/148‑1 (MQ=255)
                                                                                                                gTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAcc                                >  2:1983678/1‑148 (MQ=255)
                                                                                                                 tttGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCt                               <  2:1061105/148‑1 (MQ=255)
                                                                                                                  ttGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtt                              >  2:1486500/1‑148 (MQ=255)
                                                                                                                   tGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCttt                             <  2:1781136/148‑1 (MQ=255)
                                                                                                                   tGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCttt                             <  2:3364864/148‑1 (MQ=255)
                                                                                                                    gTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttc                            >  1:1154002/1‑148 (MQ=255)
                                                                                                                      cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcat                          >  1:1891871/1‑148 (MQ=255)
                                                                                                                      cATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcat                          <  2:1254491/148‑1 (MQ=255)
                                                                                                                        tCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcattt                        >  2:539296/1‑148 (MQ=255)
                                                                                                                         cgcgGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAACtttcatttc                       >  2:402882/1‑148 (MQ=255)
                                                                                                                          gcgggcggGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCtttcatttca                      <  1:1369198/148‑1 (MQ=255)
                                                                                                                              gcggggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGt                  <  1:1671902/148‑1 (MQ=255)
                                                                                                                                 gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa               <  1:2834154/148‑1 (MQ=255)
                                                                                                                                 gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa               <  2:3069621/148‑1 (MQ=255)
                                                                                                                                 gggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCa               >  2:3012934/1‑148 (MQ=255)
                                                                                                                                  ggcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCAt              >  2:144780/1‑148 (MQ=255)
                                                                                                                                   gcgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATg             <  1:3714558/148‑1 (MQ=255)
                                                                                                                                    cgACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGc            <  2:3726122/148‑1 (MQ=255)
                                                                                                                                      actgCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtt          <  1:160964/145‑1 (MQ=255)
                                                                                                                                      aCGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtt          <  2:17019/148‑1 (MQ=255)
                                                                                                                                        ggCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCtttt        <  1:3692599/148‑1 (MQ=255)
                                                                                                                                          ccACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTGTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTtct      <  2:1154207/148‑1 (MQ=255)
                                                                                                                                            aCCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTcc    >  2:3502122/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCAC  >  CP009273/2711610‑2711899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCA  >  CP009273/2711607‑2711901
                                                                                                                                                    |                                                                                                                                                  
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAA                                                                                                                                                   >  SRR19973069.17261/1‑150 (MQ=60)
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAA                                                                                                                                                   >  SRR19973069.16298/1‑150 (MQ=60)
 AACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAG                                                                                                                                                  >  SRR19973069.1805646/1‑150 (MQ=60)
    tttcacaccttaagcccccgtgagcggctttgcccggcgcagcaaccccgggcgcgcggcaacccgggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCAC                                                                                                                                               <  SRR19973069.2100277/80‑1 (MQ=60)
      TTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGA                                                                                                                                             >  SRR19973069.3386876/1‑150 (MQ=60)
         CCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGAT                                                                                                                                          >  SRR19973069.1581928/1‑150 (MQ=60)
          CACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATT                                                                                                                                         >  SRR19973069.3103616/1‑150 (MQ=60)
           cgtgaggccccggggatcggccttggcgggcgtcacaccccccggcgcggggcaaccggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTT                                                                                                                                        <  SRR19973069.909596/87‑1 (MQ=60)
           ACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTT                                                                                                                                        >  SRR19973069.3750502/1‑150 (MQ=60)
           ACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTT                                                                                                                                        >  SRR19973069.3750431/1‑150 (MQ=60)
            CTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTT                                                                                                                                       >  SRR19973069.3160079/1‑150 (MQ=60)
               agcccccggtgagcggccttgcccggcgacacacccCCCGGCGCGCGGCAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCT                                                                                                                                    <  SRR19973069.539045/114‑1 (MQ=60)
                TGCCACGGTGAGCGGCCTTTCCCGGCGACACACCCCCCGGCGCGGGGGAAACCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTG                                                                                                                                   <  SRR19973069.2845297/150‑1 (MQ=60)
                 GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGG                                                                                                                                  >  SRR19973069.1843728/1‑150 (MQ=60)
                 GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGG                                                                                                                                  >  SRR19973069.1843783/1‑150 (MQ=60)
                 GCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGG                                                                                                                                  >  SRR19973069.2428214/1‑150 (MQ=60)
                   CACCGTGATGGGCCTTGGCCGGCGTCACATCCCCGGGCGCGCGGTAACCCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGAC                                                                                                                                <  SRR19973069.3339672/150‑1 (MQ=60)
                     CCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGC                                                                                                                              >  SRR19973069.26656/1‑150 (MQ=60)
                       GTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCC                                                                                                                            >  SRR19973069.1037771/1‑150 (MQ=60)
                         GATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGC                                                                                                                          >  SRR19973069.3029428/1‑150 (MQ=60)
                            CGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCC                                                                                                                       >  SRR19973069.1068490/1‑150 (MQ=60)
                            CGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCC                                                                                                                       >  SRR19973069.1068707/1‑150 (MQ=60)
                                ttgggccggcgccacacccccgggcgcgcggcacccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTT                                                                                                                   <  SRR19973069.163696/108‑1 (MQ=60)
                                ctttgccggcgtctcacccccgggggcgcgtcaacccggGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTT                                                                                                                   <  SRR19973069.2106408/111‑1 (MQ=60)
                                ctttcccggggtcaccccccccggcgcggggcacccgggGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTT                                                                                                                   <  SRR19973069.2487518/111‑1 (MQ=60)
                                  ttcccggcgtctcacccccgggcgcggggtaaccggcgccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCC                                                                                                                 <  SRR19973069.2151828/110‑1 (MQ=60)
                                          GTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGC                                                                                                         >  SRR19973069.738495/1‑150 (MQ=60)
                                            CTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGG                                                                                                       >  SRR19973069.1677174/1‑150 (MQ=60)
                                              CATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                     >  SRR19973069.1956908/1‑150 (MQ=60)
                                              CATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                     >  SRR19973069.15071/1‑150 (MQ=60)
                                              CATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                     >  SRR19973069.2171490/1‑150 (MQ=60)
                                              CATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                     >  SRR19973069.15083/1‑150 (MQ=60)
                                              CATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGT                                                                                                     >  SRR19973069.1305665/1‑150 (MQ=60)
                                               cccccccggcggggggaaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTT                                                                                                    <  SRR19973069.1306176/123‑1 (MQ=60)
                                                   ccgggggcgcgggaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                <  SRR19973069.737575/127‑1 (MQ=60)
                                                   ccgggcgcgcgggaaccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                <  SRR19973069.737323/127‑1 (MQ=60)
                                                   CCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGG                                                                                                >  SRR19973069.2689701/1‑150 (MQ=60)
                                                    CCGGCGCGCGGTAAACCGGGCCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGG                                                                                               <  SRR19973069.941743/150‑1 (MQ=60)
                                                      GGCGCGGGGCAACCCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTA                                                                                             <  SRR19973069.3523633/150‑1 (MQ=60)
                                                          ggcggcaaccggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.2303930/134‑1 (MQ=60)
                                                          cgcggtaaccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.3594274/134‑1 (MQ=60)
                                                          cgcggcaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.1492812/134‑1 (MQ=60)
                                                          cgcggcaccccggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.3594629/134‑1 (MQ=60)
                                                          cgcggcaaccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.3594619/134‑1 (MQ=60)
                                                          cgcgccacccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTT                                                                                         <  SRR19973069.1307928/134‑1 (MQ=60)
                                                           gcgccaccccgccccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTT                                                                                        <  SRR19973069.1392940/135‑1 (MQ=60)
                                                           GCGGGAAACGGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTT                                                                                        <  SRR19973069.204804/150‑1 (MQ=60)
                                                           GCGGCAACCCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTT                                                                                        <  SRR19973069.2146931/150‑1 (MQ=60)
                                                             ggtaaacggggccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAT                                                                                      <  SRR19973069.3657709/137‑1 (MQ=60)
                                                             ggcaaccggtgccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTAT                                                                                      <  SRR19973069.250538/137‑1 (MQ=60)
                                                              GGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATC                                                                                     >  SRR19973069.652187/1‑150 (MQ=60)
                                                                acccgggcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAG                                                                                   <  SRR19973069.3535647/140‑1 (MQ=60)
                                                                  ACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAA                                                                                 >  SRR19973069.2200445/1‑150 (MQ=60)
                                                                   cggtcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAT                                                                                <  SRR19973069.1118660/143‑1 (MQ=60)
                                                                   cgggGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAT                                                                                <  SRR19973069.1119210/146‑1 (MQ=60)
                                                                   CCGGGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAAT                                                                                <  SRR19973069.3600008/150‑1 (MQ=60)
                                                                    CGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATC                                                                               >  SRR19973069.3107949/1‑150 (MQ=60)
                                                                      gcccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCAT                                                                             <  SRR19973069.179038/146‑1 (MQ=60)
                                                                      cgccCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCAT                                                                             <  SRR19973069.18486/146‑1 (MQ=60)
                                                                        CCCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCA                                                                           <  SRR19973069.642030/150‑1 (MQ=60)
                                                                              GGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTG                                                                     <  SRR19973069.2022028/150‑1 (MQ=60)
                                                                              GGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTG                                                                     >  SRR19973069.1877764/1‑150 (MQ=60)
                                                                              GGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTG                                                                     >  SRR19973069.3827808/1‑150 (MQ=60)
                                                                                CGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCG                                                                   >  SRR19973069.1032845/1‑150 (MQ=60)
                                                                                CGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCG                                                                   >  SRR19973069.2955476/1‑150 (MQ=60)
                                                                                CGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCG                                                                   >  SRR19973069.3122623/1‑150 (MQ=60)
                                                                                CGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCG                                                                   >  SRR19973069.1205161/1‑150 (MQ=60)
                                                                                CGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCG                                                                   >  SRR19973069.2803349/1‑150 (MQ=60)
                                                                                 GAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGG                                                                  >  SRR19973069.2121562/1‑150 (MQ=60)
                                                                                     CTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACAT                                                              >  SRR19973069.3556012/1‑150 (MQ=60)
                                                                                     CTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACAT                                                              >  SRR19973069.3013601/1‑150 (MQ=60)
                                                                                     CTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACAT                                                              >  SRR19973069.604228/1‑150 (MQ=60)
                                                                                     CTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACAT                                                              >  SRR19973069.1765147/1‑150 (MQ=60)
                                                                                             TTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACT                                                      >  SRR19973069.3402810/1‑150 (MQ=60)
                                                                                               GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                    <  SRR19973069.409119/150‑1 (MQ=60)
                                                                                               GCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTT                                                    <  SRR19973069.1009826/150‑1 (MQ=60)
                                                                                                 GGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGA                                                  >  SRR19973069.3710231/1‑150 (MQ=60)
                                                                                                     ACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTT                                              <  SRR19973069.1321444/150‑1 (MQ=60)
                                                                                                      CGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTG                                             <  SRR19973069.1167589/150‑1 (MQ=60)
                                                                                                      CGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTG                                             <  SRR19973069.1067836/150‑1 (MQ=60)
                                                                                                       GGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTCCGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGC                                            >  SRR19973069.1175148/1‑150 (MQ=60)
                                                                                                        GGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCC                                           <  SRR19973069.1291747/150‑1 (MQ=60)
                                                                                                           CTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTCCGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCT                                        >  SRR19973069.3600202/1‑150 (MQ=60)
                                                                                                            TGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCGT                                       >  SRR19973069.1626067/1‑150 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      <  SRR19973069.3063005/150‑1 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      >  SRR19973069.1717312/1‑150 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      >  SRR19973069.1828470/1‑150 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      >  SRR19973069.1827635/1‑150 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      <  SRR19973069.2748181/150‑1 (MQ=60)
                                                                                                             GACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGAGTTTTTTTGGTACGTTTATAAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTT                                      <  SRR19973069.3770178/150‑1 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     <  SRR19973069.1117714/150‑1 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     <  SRR19973069.529745/150‑1 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     <  SRR19973069.1117471/150‑1 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     <  SRR19973069.2458791/150‑1 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     >  SRR19973069.3646743/1‑150 (MQ=60)
                                                                                                              ACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTT                                     <  SRR19973069.1372261/150‑1 (MQ=60)
                                                                                                                TCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAC                                   <  SRR19973069.3707150/150‑1 (MQ=60)
                                                                                                                TCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTAC                                   <  SRR19973069.1802943/150‑1 (MQ=60)
                                                                                                                 CTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACC                                  <  SRR19973069.2893839/150‑1 (MQ=60)
                                                                                                                     TTGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTC                              >  SRR19973069.1162049/1‑150 (MQ=60)
                                                                                                                      TGTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCA                             <  SRR19973069.2963600/150‑1 (MQ=60)
                                                                                                                       GTCATCGCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCAT                            >  SRR19973069.1904989/1‑150 (MQ=60)
                                                                                                                             GCGGGCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATC                      <  SRR19973069.1378724/150‑1 (MQ=60)
                                                                                                                                 GCGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTC                  <  SRR19973069.1683522/150‑1 (MQ=60)
                                                                                                                                  CGGGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTTCCTTTCATTTCATCGTTCA                 >  SRR19973069.3087465/1‑150 (MQ=60)
                                                                                                                                    GGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATG               <  SRR19973069.3593153/150‑1 (MQ=60)
                                                                                                                                    GGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATG               <  SRR19973069.2852814/150‑1 (MQ=60)
                                                                                                                                    GGGCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATG               <  SRR19973069.2852865/150‑1 (MQ=60)
                                                                                                                                      GCGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCT             <  SRR19973069.3738815/150‑1 (MQ=60)
                                                                                                                                       CGACGGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTT            <  SRR19973069.546271/150‑1 (MQ=60)
                                                                                                                                         ACTGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTT          <  SRR19973069.162366/150‑1 (MQ=60)
                                                                                                                                           GGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCT        <  SRR19973069.3716766/150‑1 (MQ=60)
                                                                                                                                           GGCCACCGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCT        <  SRR19973069.3716744/150‑1 (MQ=60)
                                                                                                                                                 CGTAAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCGTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCA  >  SRR19973069.1799898/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                  
AAACTTTTACCACTGATGCCACCGTGATCGGCCTTTTCCGGCGTCTCATCACCCGGCGCGCGGGAAACCGTGCGCCACGGCGAATCTTCCCACTTGCGGTCACGGGGCTGACTCTGTTTGTCATCGCGGGCGGGGCGACGGCCACCGTCAGCACGAGATTTTCCTGGACGCCCGCCCCCTTTCCCGGTACGCGGGTTGTGGGTACGTTTATCAGAATCATCATCACTGCGGACATACATCACTTTGACCTTGCCGCTTTTACCTTTCATTTCATCGTTCATGCTTTTCTCCACCA  >  CP009273/2711607‑2711901

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑