Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,808,907 C→A 100% E298* (GAA→TAA)  gshA ← glutamate‑cysteine ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,808,9070CA99.5% 626.6 / ‑4.3 191E298* (GAA→TAA) gshAglutamate‑cysteine ligase
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (87/103);  total (87/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 192 total aligned reads displayed.

TGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAC  >  CP009273/2808762‑2809050
                                                                                                                                                 |                                                                                                                                               
tgctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGt                                                                                                                                               >  1:2380542/1‑148 (MQ=255)
tgctgTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGt                                                                                                                                               >  1:3381491/1‑148 (MQ=255)
    gTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTc                                                                                                                                           <  2:1934480/148‑1 (MQ=255)
     ttCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCa                                                                                                                                          <  2:3119482/148‑1 (MQ=255)
     ttCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCa                                                                                                                                          >  1:622801/1‑148 (MQ=255)
     ttCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCa                                                                                                                                          <  1:1372156/148‑1 (MQ=255)
        aTCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATc                                                                                                                                       <  2:1611437/148‑1 (MQ=255)
         tCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCt                                                                                                                                      >  2:901716/1‑148 (MQ=255)
          cTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTg                                                                                                                                     <  2:2400510/148‑1 (MQ=255)
           tACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGc                                                                                                                                    <  1:1204194/148‑1 (MQ=255)
            acacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCa                                                                                                                                   <  2:1326/148‑1 (MQ=255)
             cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCaa                                                                                                                                  <  2:1634768/148‑1 (MQ=255)
             cacCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCaa                                                                                                                                  >  1:214323/1‑148 (MQ=255)
               ccAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAaca                                                                                                                                >  2:365300/1‑148 (MQ=255)
                 aaTCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACg                                                                                                                              <  1:135899/148‑1 (MQ=255)
                   tCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGtt                                                                                                                            >  2:1660854/1‑148 (MQ=255)
                    cGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTg                                                                                                                           <  2:2605681/148‑1 (MQ=255)
                     ggCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGGCGCTGCGGGTAACTCGTTTTGGACTAATCGGCGCGTACAGTTAGTTGTCAATCTGCAACACGTTGc                                                                                                                          <  2:3367280/148‑1 (MQ=255)
                          gatcGGGTTGAGGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACCCGTTGCTGTTg                                                                                                                     <  1:1326158/145‑1 (MQ=255)
                          gaACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTg                                                                                                                     <  1:3072845/148‑1 (MQ=255)
                             cGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGAtt                                                                                                                  <  1:3328347/148‑1 (MQ=255)
                                gTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCTGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGc                                                                                                               >  1:1044152/1‑148 (MQ=255)
                                    aTGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGcc                                                                                                           <  2:1235352/148‑1 (MQ=255)
                                      gTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCctc                                                                                                         <  1:623551/148‑1 (MQ=255)
                                        ccAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCtt                                                                                                       <  2:1078844/148‑1 (MQ=255)
                                            cGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCg                                                                                                   >  2:612400/1‑148 (MQ=255)
                                            cGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCg                                                                                                   >  1:2265457/1‑148 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc                                                                                                 >  2:490834/1‑148 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc                                                                                                 <  1:3239530/148‑1 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGAGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc                                                                                                 <  1:1592518/148‑1 (MQ=255)
                                              aaCGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGAGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTc                                                                                                 <  1:1592243/148‑1 (MQ=255)
                                                 tcACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCTTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCTATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTTCCGTCttt                                                                                              <  2:572452/147‑1 (MQ=255)
                                                 gCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCttt                                                                                              <  2:2336884/148‑1 (MQ=255)
                                                  cACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtc                                                                                             <  1:34810/148‑1 (MQ=255)
                                                    cTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtctc                                                                                           >  2:1567147/1‑148 (MQ=255)
                                                    cTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTtctc                                                                                           >  2:1758402/1‑148 (MQ=255)
                                                     ttCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                          >  2:2077087/1‑148 (MQ=255)
                                                     ttCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                          >  2:2078539/1‑148 (MQ=255)
                                                     ttCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCa                                                                                          >  2:3456265/1‑148 (MQ=255)
                                                        aaTATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGGACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATa                                                                                       >  1:2884738/1‑148 (MQ=255)
                                                         atatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAc                                                                                      >  1:690099/1‑148 (MQ=255)
                                                         atatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAc                                                                                      <  2:250574/148‑1 (MQ=255)
                                                          tatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAcc                                                                                     >  1:315456/1‑148 (MQ=255)
                                                          tatatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAcc                                                                                     >  2:820738/1‑148 (MQ=255)
                                                           atatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCa                                                                                    <  1:1761304/148‑1 (MQ=255)
                                                           atatTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCa                                                                                    >  1:1323885/1‑148 (MQ=255)
                                                             atTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAt                                                                                  <  2:150496/148‑1 (MQ=255)
                                                             atTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAt                                                                                  <  2:1152723/148‑1 (MQ=255)
                                                              ttCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATc                                                                                 >  2:679025/1‑148 (MQ=255)
                                                              ttCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATc                                                                                 >  2:3412936/1‑148 (MQ=255)
                                                               tCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCt                                                                                <  2:2322963/148‑1 (MQ=255)
                                                               tCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCt                                                                                >  2:1223740/1‑148 (MQ=255)
                                                               tCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCt                                                                                <  2:2472729/148‑1 (MQ=255)
                                                                cAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCtt                                                                               <  1:884932/148‑1 (MQ=255)
                                                                  aTGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTcg                                                                             >  1:2989060/1‑148 (MQ=255)
                                                                    gccgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTcgcg                                                                           >  1:1180823/1‑148 (MQ=255)
                                                                    gccgccACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTcgcg                                                                           >  2:3490380/1‑148 (MQ=255)
                                                                         cACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActc                                                                      <  2:1723845/148‑1 (MQ=255)
                                                                          aCGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActct                                                                     >  1:849760/1‑148 (MQ=255)
                                                                          aCGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTActct                                                                     >  1:74220/1‑148 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                    <  1:293570/148‑1 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                    >  1:2630349/1‑148 (MQ=255)
                                                                           cGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCtt                                                                    >  1:3184838/1‑148 (MQ=255)
                                                                            gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTc                                                                   <  1:2407214/148‑1 (MQ=255)
                                                                            gTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTc                                                                   <  1:3118399/148‑1 (MQ=255)
                                                                              aaCAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCg                                                                 <  1:1746440/148‑1 (MQ=255)
                                                                              aaCAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTATTCCg                                                                 <  2:1037478/148‑1 (MQ=255)
                                                                                     cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGt                                                          >  1:2329415/1‑148 (MQ=255)
                                                                                     cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGt                                                          >  1:2165224/1‑148 (MQ=255)
                                                                                     cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGt                                                          >  1:569467/1‑148 (MQ=255)
                                                                                     cATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGt                                                          >  1:2823989/1‑148 (MQ=255)
                                                                                         aGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTg                                                      <  1:1567147/148‑1 (MQ=255)
                                                                                           aaGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAt                                                    >  1:1433776/1‑148 (MQ=255)
                                                                                           aaGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAt                                                    >  2:1746623/1‑148 (MQ=255)
                                                                                             ggCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTg                                                  >  2:2929686/1‑148 (MQ=255)
                                                                                              gCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGc                                                 <  1:2171182/148‑1 (MQ=255)
                                                                                              gCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGc                                                 <  1:1252109/148‑1 (MQ=255)
                                                                                                  cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                             <  2:3469966/148‑1 (MQ=255)
                                                                                                  cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                             >  1:3206270/1‑148 (MQ=255)
                                                                                                  cTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGt                                             >  2:2878831/1‑148 (MQ=255)
                                                                                                   tCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGtt                                            <  2:3270116/148‑1 (MQ=255)
                                                                                                    cgccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGttt                                           <  1:275104/148‑1 (MQ=255)
                                                                                                     gccgcTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTa                                          <  1:1167694/148‑1 (MQ=255)
                                                                                                          tGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGcc                                     <  1:3295499/148‑1 (MQ=255)
                                                                                                            cGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCg                                   >  2:2574892/1‑148 (MQ=255)
                                                                                                                   cgcgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTa                            >  2:3348665/1‑148 (MQ=255)
                                                                                                                     cgTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACt                          <  1:508057/148‑1 (MQ=255)
                                                                                                                        tttGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGt                       >  1:3046803/1‑148 (MQ=255)
                                                                                                                          tGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTaa                     <  1:1009490/148‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                  <  1:736779/148‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                  <  2:1433776/148‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                  <  2:554185/148‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                  <  1:2596379/148‑1 (MQ=255)
                                                                                                                             aCGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGa                  <  1:1658642/148‑1 (MQ=255)
                                                                                                                                 aTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGCTGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGt              >  1:2494411/1‑148 (MQ=255)
                                                                                                                                 aTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGt              <  2:2109521/148‑1 (MQ=255)
                                                                                                                                          tACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTaa     >  1:296607/1‑148 (MQ=255)
                                                                                                                                          tACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTaa     >  1:296559/1‑148 (MQ=255)
                                                                                                                                            cAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGACGGTAATa   >  2:1542101/1‑148 (MQ=255)
                                                                                                                                            cAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATa   >  2:1542098/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAC  >  CP009273/2808762‑2809050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAT  >  CP009273/2808759‑2809056
                                                                                                                                                    |                                                                                                                                                     
ACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAG                                                                                                                                                      <  SRR19973065.3065989/150‑1 (MQ=60)
 CCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGT                                                                                                                                                     >  SRR19973065.2395443/1‑150 (MQ=60)
 CCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGT                                                                                                                                                     >  SRR19973065.3402167/1‑150 (MQ=60)
  CTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCACTGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTT                                                                                                                                                    <  SRR19973065.319526/150‑1 (MQ=60)
  CTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTT                                                                                                                                                    <  SRR19973065.319563/150‑1 (MQ=60)
      TGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCA                                                                                                                                                >  SRR19973065.667742/1‑150 (MQ=60)
      TGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCA                                                                                                                                                >  SRR19973065.626825/1‑150 (MQ=60)
      TGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCA                                                                                                                                                >  SRR19973065.3536834/1‑150 (MQ=60)
        TTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAAT                                                                                                                                              <  SRR19973065.1381212/150‑1 (MQ=60)
         TCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATC                                                                                                                                             >  SRR19973065.2539642/1‑150 (MQ=60)
         TCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATC                                                                                                                                             >  SRR19973065.3340164/1‑150 (MQ=60)
              TACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAA                                                                                                                                        >  SRR19973065.215941/1‑150 (MQ=60)
              TACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAA                                                                                                                                        <  SRR19973065.1211929/150‑1 (MQ=60)
                    AATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTT                                                                                                                                  <  SRR19973065.137064/150‑1 (MQ=60)
                    AATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTT                                                                                                                                  <  SRR19973065.137232/150‑1 (MQ=60)
                       CGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATGCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGACTCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCT                                                                                                                               <  SRR19973065.947068/150‑1 (MQ=60)
                            AGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGA                                                                                                                          >  SRR19973065.880053/1‑150 (MQ=60)
                             GATCGGGTTGAGGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACCCGTTGCTGTTGAT                                                                                                                         <  SRR19973065.1334921/150‑1 (MQ=60)
                             GAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGAT                                                                                                                         <  SRR19973065.3091625/150‑1 (MQ=60)
                             GAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGAT                                                                                                                         <  SRR19973065.1334809/150‑1 (MQ=60)
                               ACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTT                                                                                                                       >  SRR19973065.2999497/1‑150 (MQ=60)
                                CGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTG                                                                                                                      <  SRR19973065.3348655/150‑1 (MQ=60)
                                 GGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCTGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGC                                                                                                                     >  SRR19973065.1051054/1‑150 (MQ=60)
                                  GGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCA                                                                                                                    >  SRR19973065.1345459/1‑150 (MQ=60)
                                       ATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTC                                                                                                               >  SRR19973065.123544/1‑150 (MQ=60)
                                         GTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTT                                                                                                             <  SRR19973065.627588/150‑1 (MQ=60)
                                         GTCCAGCGAACGCACTTCAATATATTCAAGGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTT                                                                                                             <  SRR19973065.2400944/150‑1 (MQ=60)
                                             AGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCG                                                                                                         >  SRR19973065.2279775/1‑150 (MQ=60)
                                               CGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTC                                                                                                       >  SRR19973065.612008/1‑150 (MQ=60)
                                                GAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCT                                                                                                      <  SRR19973065.1098252/150‑1 (MQ=60)
                                                 AACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTT                                                                                                     <  SRR19973065.3369148/150‑1 (MQ=60)
                                                 AACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTT                                                                                                     <  SRR19973065.3259045/150‑1 (MQ=60)
                                                 AACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTT                                                                                                     <  SRR19973065.3258822/150‑1 (MQ=60)
                                                 AACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGAGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTT                                                                                                     <  SRR19973065.1602881/150‑1 (MQ=60)
                                                 AACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGAGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTT                                                                                                     <  SRR19973065.1603157/150‑1 (MQ=60)
                                                   CGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTC                                                                                                   >  SRR19973065.3425724/1‑150 (MQ=60)
                                                     CACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTC                                                                                                 <  SRR19973065.35244/150‑1 (MQ=60)
                                                         TCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATA                                                                                             <  SRR19973065.2882835/150‑1 (MQ=60)
                                                         TCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGGACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATA                                                                                             >  SRR19973065.2902382/1‑150 (MQ=60)
                                                          CAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATAC                                                                                            >  SRR19973065.694692/1‑150 (MQ=60)
                                                           AATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACC                                                                                           >  SRR19973065.3266965/1‑150 (MQ=60)
                                                           AATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACC                                                                                           >  SRR19973065.317603/1‑150 (MQ=60)
                                                            ATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCA                                                                                          >  SRR19973065.1332640/1‑150 (MQ=60)
                                                              ATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAT                                                                                        <  SRR19973065.1772830/150‑1 (MQ=60)
                                                              ATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAT                                                                                        <  SRR19973065.1772865/150‑1 (MQ=60)
                                                              ATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAAT                                                                                        <  SRR19973065.1815798/150‑1 (MQ=60)
                                                                ATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCT                                                                                      <  SRR19973065.2267075/150‑1 (MQ=60)
                                                                   CAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCG                                                                                   >  SRR19973065.3007358/1‑150 (MQ=60)
                                                                   CAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCG                                                                                   <  SRR19973065.890558/150‑1 (MQ=60)
                                                                     ATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCG                                                                                 >  SRR19973065.1188307/1‑150 (MQ=60)
                                                                         CGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACT                                                                             <  SRR19973065.1197488/150‑1 (MQ=60)
                                                                           CCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCT                                                                           >  SRR19973065.855266/1‑150 (MQ=60)
                                                                           CCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCT                                                                           >  SRR19973065.74985/1‑150 (MQ=60)
                                                                            CACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTT                                                                          >  SRR19973065.3204069/1‑150 (MQ=60)
                                                                            CACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTT                                                                          <  SRR19973065.1641464/150‑1 (MQ=60)
                                                                            CACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTT                                                                          <  SRR19973065.3066154/150‑1 (MQ=60)
                                                                            CACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTT                                                                          >  SRR19973065.3204130/1‑150 (MQ=60)
                                                                            CACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTT                                                                          >  SRR19973065.2646613/1‑150 (MQ=60)
                                                                              CGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCC                                                                        <  SRR19973065.295465/150‑1 (MQ=60)
                                                                               GTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCG                                                                       <  SRR19973065.2422360/150‑1 (MQ=60)
                                                                               GTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCG                                                                       <  SRR19973065.3137404/150‑1 (MQ=60)
                                                                                 AACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGAT                                                                     <  SRR19973065.1757951/150‑1 (MQ=60)
                                                                                      CGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCGGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGT                                                                >  SRR19973065.2345252/1‑150 (MQ=60)
                                                                                      CGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGT                                                                >  SRR19973065.2179136/1‑150 (MQ=60)
                                                                                      CGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGT                                                                >  SRR19973065.2343987/1‑150 (MQ=60)
                                                                                      CGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGT                                                                >  SRR19973065.573069/1‑150 (MQ=60)
                                                                                      CGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGT                                                                >  SRR19973065.2841425/1‑150 (MQ=60)
                                                                                        CATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTT                                                              <  SRR19973065.834513/150‑1 (MQ=60)
                                                                                         ATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTT                                                             >  SRR19973065.3158622/1‑150 (MQ=60)
                                                                                            AGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAT                                                          >  SRR19973065.1443089/1‑150 (MQ=60)
                                                                                            AGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAT                                                          >  SRR19973065.1443950/1‑150 (MQ=60)
                                                                                            AGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAT                                                          <  SRR19973065.1577564/150‑1 (MQ=60)
                                                                                            AGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGAT                                                          <  SRR19973065.627201/150‑1 (MQ=60)
                                                                                              AAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTG                                                        <  SRR19973065.2832259/150‑1 (MQ=60)
                                                                                                 GCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCT                                                     <  SRR19973065.1260351/150‑1 (MQ=60)
                                                                                                 GCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCT                                                     <  SRR19973065.2442936/150‑1 (MQ=60)
                                                                                                 GCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCT                                                     <  SRR19973065.2185116/150‑1 (MQ=60)
                                                                                                   GACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGT                                                   >  SRR19973065.1594628/1‑150 (MQ=60)
                                                                                                   GACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGT                                                   >  SRR19973065.2751456/1‑150 (MQ=60)
                                                                                                   GACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGT                                                   >  SRR19973065.1282982/1‑150 (MQ=60)
                                                                                                   GACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGT                                                   >  SRR19973065.3225568/1‑150 (MQ=60)
                                                                                                       CGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAA                                               <  SRR19973065.276949/150‑1 (MQ=60)
                                                                                                        GCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAG                                              <  SRR19973065.1175144/150‑1 (MQ=60)
                                                                                                        GCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAG                                              <  SRR19973065.1175193/150‑1 (MQ=60)
                                                                                                        GCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAG                                              <  SRR19973065.2935588/150‑1 (MQ=60)
                                                                                                          CGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGC                                            >  SRR19973065.1434103/1‑150 (MQ=60)
                                                                                                             TGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCG                                         <  SRR19973065.3315567/150‑1 (MQ=60)
                                                                                                               CGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTTCCTGTTTAAGGCCCGCT                                       >  SRR19973065.2182380/1‑150 (MQ=60)
                                                                                                                GGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTA                                      <  SRR19973065.3109759/150‑1 (MQ=60)
                                                                                                                GGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTA                                      >  SRR19973065.2976399/1‑150 (MQ=60)
                                                                                                                GGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTA                                      <  SRR19973065.2291302/150‑1 (MQ=60)
                                                                                                                        CGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCG                              <  SRR19973065.511436/150‑1 (MQ=60)
                                                                                                                        CGTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCG                              <  SRR19973065.513694/150‑1 (MQ=60)
                                                                                                                         GTTTTGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGT                             >  SRR19973065.3065448/1‑150 (MQ=60)
                                                                                                                             TGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAG                         <  SRR19973065.1016184/150‑1 (MQ=60)
                                                                                                                             TGGACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAG                         <  SRR19973065.2168855/150‑1 (MQ=60)
                                                                                                                                ACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATC                      <  SRR19973065.1669751/150‑1 (MQ=60)
                                                                                                                                ACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATC                      <  SRR19973065.741705/150‑1 (MQ=60)
                                                                                                                                ACGAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATC                      <  SRR19973065.2612516/150‑1 (MQ=60)
                                                                                                                                  GAATCGGCGCGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGCTGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGT                    >  SRR19973065.2510106/1‑150 (MQ=60)
                                                                                                                                           CGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAA           >  SRR19973065.3028140/1‑150 (MQ=60)
                                                                                                                                           CGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAA           >  SRR19973065.298517/1‑150 (MQ=60)
                                                                                                                                           CGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAA           >  SRR19973065.298479/1‑150 (MQ=60)
                                                                                                                                           CGTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAA           >  SRR19973065.298467/1‑150 (MQ=60)
                                                                                                                                            GTACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAAT          <  SRR19973065.735717/150‑1 (MQ=60)
                                                                                                                                              ACAGTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAC        >  SRR19973065.1748445/1‑150 (MQ=60)
                                                                                                                                                 GTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAA     >  SRR19973065.318123/1‑150 (MQ=60)
                                                                                                                                                 GTTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCACAATACCAATCTTCGCGTACTCTTACGATGGCGATTTGATTGCCTGTTTAATGCCCGCTACGGAATCGTAAAGATCGTTTAATGTAATACCAA     >  SRR19973065.317372/1‑150 (MQ=60)
                                                                                                                                                  TTAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAG    <  SRR19973065.2638801/150‑1 (MQ=60)
                                                                                                                                                   TAGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGA   >  SRR19973065.2099209/1‑150 (MQ=60)
                                                                                                                                                    AGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAT  <  SRR19973065.3003690/150‑1 (MQ=60)
                                                                                                                                                    AGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAT  <  SRR19973065.3003693/150‑1 (MQ=60)
                                                                                                                                                    AGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAT  >  SRR19973065.129993/1‑150 (MQ=60)
                                                                                                                                                    AGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATAACAAGAT  >  SRR19973065.2430880/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
ACCTGCTGTTCATCTACACCAATCGGCGAGAACGGGTTGATGTCCAGCGAACGCACTTCAATATATTCAATGCCGCCACGTAACAGCGCATCAGAAGGCGACTCGCCGCTGCGGGTAACGCGTTTTGGACGAATCGGCGCGTACAGTTCGTTTTCAATCTGCAACACGTTGCTGTTGATTTGCAGCCTCTTACCGTCTTTCTCAATACCAATCTTCGCGTACTCTTCCGATGGCGTTTTGATTGCCTGTTTAAGGCCCGCTACGTACTCGTAAAGATCGTTGAAGGTAATACCAAGAT  >  CP009273/2808759‑2809056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑