Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F434 I0 R1	1130	215.9	7031662	97.1%	6827743	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	3,261,001	G→A	100%	intergenic (+44/‑212)	tdcR → / → yhaB	L‑threonine dehydratase operon activator protein/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	3,261,001	0	G	A	99.6%	828.3 / ‑4.5	248	intergenic (+44/‑212)	tdcR/yhaB	L‑threonine dehydratase operon activator protein/uncharacterized protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (113/134); total (114/134)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 248 total aligned reads displayed.

AAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA  >  CP009273/3260856‑3261144

  aaTTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATca                                                                                                                                             >  1:3315662/1‑148 (MQ=255)

    tttCGAATCCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATcaca                                                                                                                                           <  2:1904685/148‑1 (MQ=255)

     ttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaa                                                                                                                                          <  2:2259774/148‑1 (MQ=255)

     ttCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaa                                                                                                                                          >  2:1266878/1‑148 (MQ=255)

       cGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACaaaa                                                                                                                                        <  1:2175486/148‑1 (MQ=255)

           tACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaa                                                                                                                                    <  1:2467236/148‑1 (MQ=255)

           tACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaa                                                                                                                                    <  1:704175/148‑1 (MQ=255)

            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   <  1:132741/148‑1 (MQ=255)

            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   >  2:2343101/1‑148 (MQ=255)

            aCATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaat                                                                                                                                   <  1:2233322/148‑1 (MQ=255)

             cATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaata                                                                                                                                  >  1:1416982/1‑148 (MQ=255)

              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 >  2:2131647/1‑148 (MQ=255)

              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 >  2:560231/1‑148 (MQ=255)

              aTGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGaataa                                                                                                                                 <  2:395066/148‑1 (MQ=255)

                gTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAAtt                                                                                                                               <  1:2016470/148‑1 (MQ=255)

                  aTTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGc                                                                                                                             >  1:760404/1‑148 (MQ=255)

                  aTTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGc                                                                                                                             >  2:2567942/1‑148 (MQ=255)

                    tGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTg                                                                                                                           <  1:1518326/148‑1 (MQ=255)

                     gATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGa                                                                                                                          <  1:1607016/148‑1 (MQ=255)

                      atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGAt                                                                                                                         <  2:1465072/148‑1 (MQ=255)

                      atcatcTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGAt                                                                                                                         <  2:1642304/148‑1 (MQ=255)

                                aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATa                                                                                                               <  2:2528435/148‑1 (MQ=255)

                                aCAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATa                                                                                                               <  2:1043903/148‑1 (MQ=255)

                                 cAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATaa                                                                                                              <  2:204620/148‑1 (MQ=255)

                                  aaTTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAg                                                                                                             >  2:34718/1‑148 (MQ=255)

                                   aTTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAgg                                                                                                            >  2:2759346/1‑148 (MQ=255)

                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATCATTGCTGATCGCCGCGATAAGGTc                                                                                                          >  2:3043366/1‑148 (MQ=255)

                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc                                                                                                          <  2:3187379/148‑1 (MQ=255)

                                     tGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTc                                                                                                          >  2:2392297/1‑148 (MQ=255)

                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        >  1:667102/1‑148 (MQ=255)

                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        <  1:2770182/148‑1 (MQ=255)

                                       aTTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCag                                                                                                        <  1:642054/148‑1 (MQ=255)

                                           aCGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaa                                                                                                    >  2:641849/1‑148 (MQ=255)

                                            cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa                                                                                                   >  2:2602722/1‑148 (MQ=255)

                                            cGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaa                                                                                                   >  1:497253/1‑148 (MQ=255)

                                              tCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaaga                                                                                                 >  1:3109879/1‑148 (MQ=255)

                                                aaCTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCagacaaagaca                                                                                               <  2:3375470/148‑1 (MQ=255)

                                                  cTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaac                                                                                             >  1:2613468/1‑148 (MQ=255)

                                                   tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaaca                                                                                            <  2:298888/148‑1 (MQ=255)

                                                   tttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGacaaca                                                                                            <  2:3280506/148‑1 (MQ=255)

                                                    ttttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGAcaacaa                                                                                           <  2:3004222/148‑1 (MQ=255)

                                                     tttCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAg                                                                                          <  1:862211/148‑1 (MQ=255)

                                                        ctctTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGa                                                                                       >  1:2041491/1‑148 (MQ=255)

                                                         tcttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaa                                                                                      <  1:1686834/148‑1 (MQ=255)

                                                          cttctGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGaaa                                                                                     <  1:1982358/148‑1 (MQ=255)

                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              >  2:1406793/1‑148 (MQ=255)

                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              >  2:26718/1‑148 (MQ=255)

                                                                 cAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTcac                                                                              <  2:2736391/148‑1 (MQ=255)

                                                                    gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACaga                                                                           >  1:312324/1‑148 (MQ=255)

                                                                    gACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCAAAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACaga                                                                           >  1:312595/1‑148 (MQ=255)

                                                                     aCGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACagag                                                                          <  1:1889012/148‑1 (MQ=255)

                                                                     aCGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACagag                                                                          >  2:1275033/1‑148 (MQ=255)

                                                                        tCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCtt                                                                       <  2:1000067/148‑1 (MQ=255)

                                                                        tCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCtt                                                                       <  2:1593710/148‑1 (MQ=255)

                                                                         cATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCttt                                                                      <  1:1009976/148‑1 (MQ=255)

                                                                            tttGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGa                                                                   >  1:2687480/1‑148 (MQ=255)

                                                                              tgtgAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATc                                                                 <  1:1887955/148‑1 (MQ=255)

                                                                                 gAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGc                                                              >  1:1741961/1‑148 (MQ=255)

                                                                                  aaTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCg                                                             <  1:2126473/148‑1 (MQ=255)

                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  2:961483/148‑1 (MQ=255)

                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  2:3423673/148‑1 (MQ=255)

                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  1:117411/148‑1 (MQ=255)

                                                                                   aTGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGt                                                            <  1:1425144/148‑1 (MQ=255)

                                                                                             tttCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGa                                                  <  2:667102/148‑1 (MQ=255)

                                                                                                   tAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTg                                            <  1:2384985/148‑1 (MQ=255)

                                                                                                    aaaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGc                                           <  2:1218905/148‑1 (MQ=255)

                                                                                                    aaaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGc                                           <  1:1800683/148‑1 (MQ=255)

                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          <  1:1064409/148‑1 (MQ=255)

                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          >  1:2163011/1‑148 (MQ=255)

                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          <  1:2343101/148‑1 (MQ=255)

                                                                                                     aaTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCt                                          >  1:3055215/1‑148 (MQ=255)

                                                                                                      aTTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCtt                                         <  1:344756/148‑1 (MQ=255)

                                                                                                        tcttctCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCg                                       <  2:407442/148‑1 (MQ=255)

                                                                                                             tcCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTAc                                  <  2:1033763/148‑1 (MQ=255)

                                                                                                              ccccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATATTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACt                                 >  1:1212389/1‑148 (MQ=255)

                                                                                                               cccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTg                                <  2:2451015/148‑1 (MQ=255)

                                                                                                               cccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTg                                >  1:193689/1‑148 (MQ=255)

                                                                                                                ccTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGt                               <  1:2325260/148‑1 (MQ=255)

                                                                                                                 cTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTa                              >  2:2853198/1‑148 (MQ=255)

                                                                                                                   cATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAAc                            >  1:37592/1‑148 (MQ=255)

                                                                                                                   cATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAAc                            >  2:2800444/1‑148 (MQ=255)

                                                                                                                    aTAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACg                           <  2:1822945/148‑1 (MQ=255)

                                                                                                                       ggCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCg                        <  1:1267136/148‑1 (MQ=255)

                                                                                                                        gCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCgg                       >  2:480749/1‑148 (MQ=255)

                                                                                                                         cgacgaATAGCTTTTTGTTTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCggg                      <  1:1816916/148‑1 (MQ=255)

                                                                                                                         cgacgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCggg                      <  1:1915896/148‑1 (MQ=255)

                                                                                                                          gacgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGt                     >  1:572059/1‑148 (MQ=255)

                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACTAATAATTGCTTATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGATTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  1:956223/148‑1 (MQ=255)

                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  2:1729220/148‑1 (MQ=255)

                                                                                                                            cgaATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTaa                   <  1:3248430/148‑1 (MQ=255)

                                                                                                                               aTAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATg                >  2:236048/1‑148 (MQ=255)

                                                                                                                                tAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGa               <  1:2574440/148‑1 (MQ=255)

                                                                                                                                tAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGa               >  2:19005/1‑148 (MQ=255)

                                                                                                                                 aGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGGAAATGAg              >  2:2556856/1‑148 (MQ=255)

                                                                                                                                  gCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGc             >  2:812375/1‑148 (MQ=255)

                                                                                                                                   cATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCg            <  1:1260393/148‑1 (MQ=255)

                                                                                                                                    aTTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           >  1:304687/1‑148 (MQ=255)

                                                                                                                                    aTTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACGAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           <  2:2031009/148‑1 (MQ=255)

                                                                                                                                    aTTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGt           <  2:115840/148‑1 (MQ=255)

                                                                                                                                             tGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATa  >  1:3190722/1‑148 (MQ=255)

AAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA  >  CP009273/3260856‑3261144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  CP009273/3260852‑3261148

TTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGA                                                                                                                                                     >  SRR19973065.2939359/1‑150 (MQ=60)

TTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGA                                                                                                                                                     >  SRR19973065.2939560/1‑150 (MQ=60)

TTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGA                                                                                                                                                     >  SRR19973065.116859/1‑150 (MQ=60)

   TAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATC                                                                                                                                                  <  SRR19973065.3175573/150‑1 (MQ=60)

    AAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCA                                                                                                                                                 >  SRR19973065.3335880/1‑150 (MQ=60)

      AATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACA                                                                                                                                               <  SRR19973065.1233760/150‑1 (MQ=60)

       ATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAA                                                                                                                                              >  SRR19973065.2416240/1‑150 (MQ=60)

       ATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAA                                                                                                                                              >  SRR19973065.3385981/1‑150 (MQ=60)

       ATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAA                                                                                                                                              <  SRR19973065.1282911/150‑1 (MQ=60)

           CGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACG                                                                                                                                          <  SRR19973065.2189345/150‑1 (MQ=60)

           CGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACG                                                                                                                                          <  SRR19973065.2189439/150‑1 (MQ=60)

            GAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGA                                                                                                                                         >  SRR19973065.2903370/1‑150 (MQ=60)

            GAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGA                                                                                                                                         >  SRR19973065.1488899/1‑150 (MQ=60)

               TACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATA                                                                                                                                      <  SRR19973065.708883/150‑1 (MQ=60)

               TACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATA                                                                                                                                      <  SRR19973065.708858/150‑1 (MQ=60)

               TACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATA                                                                                                                                      >  SRR19973065.1426233/1‑150 (MQ=60)

               TACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATA                                                                                                                                      <  SRR19973065.2482785/150‑1 (MQ=60)

               TACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATA                                                                                                                                      >  SRR19973065.2108416/1‑150 (MQ=60)

                ACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAA                                                                                                                                     <  SRR19973065.2247531/150‑1 (MQ=60)

                ACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAA                                                                                                                                     <  SRR19973065.133882/150‑1 (MQ=60)

                 CATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAAT                                                                                                                                    >  SRR19973065.2545111/1‑150 (MQ=60)

                    GTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGC                                                                                                                                 <  SRR19973065.2029397/150‑1 (MQ=60)

                    GTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGC                                                                                                                                 >  SRR19973065.765471/1‑150 (MQ=60)

                    GTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGC                                                                                                                                 <  SRR19973065.2029461/150‑1 (MQ=60)

                    GTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGC                                                                                                                                 <  SRR19973065.2029517/150‑1 (MQ=60)

                     TATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCT                                                                                                                                >  SRR19973065.1644744/1‑150 (MQ=60)

                     TATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCT                                                                                                                                >  SRR19973065.2175950/1‑150 (MQ=60)

                       TTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGA                                                                                                                              >  SRR19973065.1094805/1‑150 (MQ=60)

                        TGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGAT                                                                                                                             <  SRR19973065.1528257/150‑1 (MQ=60)

                         GATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATC                                                                                                                            <  SRR19973065.1617773/150‑1 (MQ=60)

                          ATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCG                                                                                                                           <  SRR19973065.3143239/150‑1 (MQ=60)

                          ATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCG                                                                                                                           <  SRR19973065.3142852/150‑1 (MQ=60)

                             ATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCG                                                                                                                        <  SRR19973065.459050/150‑1 (MQ=60)

                                  GAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATA                                                                                                                   >  SRR19973065.307237/1‑150 (MQ=60)

                                  GAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATA                                                                                                                   >  SRR19973065.582556/1‑150 (MQ=60)

                                  GAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGAAGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATA                                                                                                                   >  SRR19973065.1921019/1‑150 (MQ=60)

                                   AACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAA                                                                                                                  >  SRR19973065.2703440/1‑150 (MQ=60)

                                     CAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGG                                                                                                                >  SRR19973065.3148547/1‑150 (MQ=60)

                                     CAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGG                                                                                                                >  SRR19973065.1391783/1‑150 (MQ=60)

                                     CAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGAGAAGG                                                                                                                >  SRR19973065.1902660/1‑150 (MQ=60)

                                      AATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGT                                                                                                               >  SRR19973065.3242087/1‑150 (MQ=60)

                                       ATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCGCAAAACGAATAATTGCTGATCGCCGCGATAAGGTC                                                                                                              >  SRR19973065.1699614/1‑150 (MQ=60)

                                         TGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAG                                                                                                            >  SRR19973065.671544/1‑150 (MQ=60)

                                           ATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGAC                                                                                                          <  SRR19973065.646267/150‑1 (MQ=60)

                                           ATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGAC                                                                                                          >  SRR19973065.2162950/1‑150 (MQ=60)

                                           ATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGAC                                                                                                          <  SRR19973065.2787389/150‑1 (MQ=60)

                                            TTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACA                                                                                                         >  SRR19973065.1772388/1‑150 (MQ=60)

                                              AACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAA                                                                                                       >  SRR19973065.500590/1‑150 (MQ=60)

                                               ACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAG                                                                                                      <  SRR19973065.186912/150‑1 (MQ=60)

                                                CGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGA                                                                                                     >  SRR19973065.3128838/1‑150 (MQ=60)

                                                CGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGA                                                                                                     >  SRR19973065.3131770/1‑150 (MQ=60)

                                                    AACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAAC                                                                                                 >  SRR19973065.2629665/1‑150 (MQ=60)

                                                         TTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGG                                                                                            <  SRR19973065.867763/150‑1 (MQ=60)

                                                          TTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGA                                                                                           >  SRR19973065.2053935/1‑150 (MQ=60)

                                                          TTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGA                                                                                           >  SRR19973065.2054559/1‑150 (MQ=60)

                                                             TCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAAT                                                                                        <  SRR19973065.1698106/150‑1 (MQ=60)

                                                              CTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATT                                                                                       <  SRR19973065.1995241/150‑1 (MQ=60)

                                                               TTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTT                                                                                      <  SRR19973065.597127/150‑1 (MQ=60)

                                                                   GACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCAC                                                                                  <  SRR19973065.1030941/150‑1 (MQ=60)

                                                                   GACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCAC                                                                                  <  SRR19973065.2794101/150‑1 (MQ=60)

                                                                     CAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAG                                                                                >  SRR19973065.3283892/1‑150 (MQ=60)

                                                                      AGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGA                                                                               >  SRR19973065.314432/1‑150 (MQ=60)

                                                                      AGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGA                                                                               >  SRR19973065.454202/1‑150 (MQ=60)

                                                                      AGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCAAAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGA                                                                               >  SRR19973065.314704/1‑150 (MQ=60)

                                                                         ACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCT                                                                            <  SRR19973065.856296/150‑1 (MQ=60)

                                                                         ACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCT                                                                            <  SRR19973065.2346971/150‑1 (MQ=60)

                                                                         ACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCT                                                                            <  SRR19973065.1901434/150‑1 (MQ=60)

                                                                           GTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTT                                                                          <  SRR19973065.1555793/150‑1 (MQ=60)

                                                                             CATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTG                                                                        <  SRR19973065.1016674/150‑1 (MQ=60)

                                                                             CATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTG                                                                        <  SRR19973065.2780488/150‑1 (MQ=60)

                                                                              ATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGA                                                                       >  SRR19973065.2704160/1‑150 (MQ=60)

                                                                                  TGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGG                                                                   <  SRR19973065.1900373/150‑1 (MQ=60)

                                                                                  TGTGAATGCAATAGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCAAAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGG                                                                   <  SRR19973065.1900381/150‑1 (MQ=60)

                                                                                   GTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGC                                                                  >  SRR19973065.682876/1‑150 (MQ=60)

                                                                                   GTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGC                                                                  >  SRR19973065.1753459/1‑150 (MQ=60)

                                                                                   GTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGC                                                                  >  SRR19973065.1754200/1‑150 (MQ=60)

                                                                                      AATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTA                                                               <  SRR19973065.2140158/150‑1 (MQ=60)

                                                                                       ATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAG                                                              <  SRR19973065.1434433/150‑1 (MQ=60)

                                                                                       ATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAG                                                              <  SRR19973065.118444/150‑1 (MQ=60)

                                                                                          CAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTTCTGATCGCAGCGATAAGGTCAGACAAAGACAACAAGGGAAAATTTCACAGAGCTTTTTATCGGCGTAGGCC                                                           >  SRR19973065.2240458/1‑150 (MQ=60)

                                                                                             TCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACA                                                        <  SRR19973065.1002496/150‑1 (MQ=60)

                                                                                              CGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAG                                                       <  SRR19973065.1426604/150‑1 (MQ=60)

                                                                                                TTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAA                                                     >  SRR19973065.657963/1‑150 (MQ=60)

                                                                                                 TTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAAT                                                    <  SRR19973065.1847320/150‑1 (MQ=60)

                                                                                                 TTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAAT                                                    <  SRR19973065.1966994/150‑1 (MQ=60)

                                                                                                 TTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAAT                                                    <  SRR19973065.1967872/150‑1 (MQ=60)

                                                                                                  TTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATG                                                   <  SRR19973065.343214/150‑1 (MQ=60)

                                                                                                  TTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATG                                                   <  SRR19973065.152364/150‑1 (MQ=60)

                                                                                                    CCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTT                                                 <  SRR19973065.563798/150‑1 (MQ=60)

                                                                                                       TAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCT                                              >  SRR19973065.2176913/1‑150 (MQ=60)

                                                                                                       TAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCT                                              <  SRR19973065.2399941/150‑1 (MQ=60)

                                                                                                       TAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCT                                              >  SRR19973065.3073894/1‑150 (MQ=60)

                                                                                                        AAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTT                                             <  SRR19973065.2378828/150‑1 (MQ=60)

                                                                                                        AAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTT                                             <  SRR19973065.1812508/150‑1 (MQ=60)

                                                                                                        AAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTT                                             <  SRR19973065.1813108/150‑1 (MQ=60)

                                                                                                         AATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTC                                            <  SRR19973065.1071462/150‑1 (MQ=60)

                                                                                                         AATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTC                                            <  SRR19973065.2357723/150‑1 (MQ=60)

                                                                                                         AATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTC                                            <  SRR19973065.2357810/150‑1 (MQ=60)

                                                                                                          ATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCG                                           <  SRR19973065.347168/150‑1 (MQ=60)

                                                                                                              TTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTA                                       >  SRR19973065.3107163/1‑150 (MQ=60)

                                                                                                              TTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTA                                       <  SRR19973065.872864/150‑1 (MQ=60)

                                                                                                              TTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTA                                       <  SRR19973065.1210895/150‑1 (MQ=60)

                                                                                                                CTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATATTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACT                                     >  SRR19973065.1220219/1‑150 (MQ=60)

                                                                                                                 TCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTG                                    >  SRR19973065.195194/1‑150 (MQ=60)

                                                                                                                 TCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTG                                    >  SRR19973065.195197/1‑150 (MQ=60)

                                                                                                                   CCCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTA                                  <  SRR19973065.2218534/150‑1 (MQ=60)

                                                                                                                    CCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAA                                 <  SRR19973065.35151/150‑1 (MQ=60)

                                                                                                                    CCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAA                                 <  SRR19973065.2339817/150‑1 (MQ=60)

                                                                                                                    CCTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAA                                 >  SRR19973065.1660735/1‑150 (MQ=60)

                                                                                                                     CTCATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAAC                                >  SRR19973065.38050/1‑150 (MQ=60)

                                                                                                                        ATAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCC                             >  SRR19973065.3121000/1‑150 (MQ=60)

                                                                                                                         TAGGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCG                            <  SRR19973065.2618882/150‑1 (MQ=60)

                                                                                                                           GGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGG                          <  SRR19973065.1275231/150‑1 (MQ=60)

                                                                                                                           GGCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGG                          <  SRR19973065.1275493/150‑1 (MQ=60)

                                                                                                                            GCGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGT                         >  SRR19973065.575669/1‑150 (MQ=60)

                                                                                                                             CGACGAATAGCTTTTTGTTTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTA                        <  SRR19973065.1828884/150‑1 (MQ=60)

                                                                                                                             CGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTA                        <  SRR19973065.1928466/150‑1 (MQ=60)

                                                                                                                             CGACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTA                        <  SRR19973065.1346372/150‑1 (MQ=60)

                                                                                                                              GACGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAA                       <  SRR19973065.2583977/150‑1 (MQ=60)

                                                                                                                                CGAATAGCATTTTGTGTTGAGAATCACAAAACTAATAATTGCTTATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGATTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAAT                     <  SRR19973065.962548/150‑1 (MQ=60)

                                                                                                                                CGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAAT                     >  SRR19973065.676380/1‑150 (MQ=60)

                                                                                                                                CGAATAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAAT                     <  SRR19973065.3268051/150‑1 (MQ=60)

                                                                                                                                    TAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGC                 <  SRR19973065.2590502/150‑1 (MQ=60)

                                                                                                                                    TAGCATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGC                 <  SRR19973065.821676/150‑1 (MQ=60)

                                                                                                                                      GCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGT               >  SRR19973065.306705/1‑150 (MQ=60)

                                                                                                                                       CATTTTGTGTTGATAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTT              <  SRR19973065.1268763/150‑1 (MQ=60)

                                                                                                                                       CATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTT              >  SRR19973065.2687331/1‑150 (MQ=60)

                                                                                                                                       CATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTT              <  SRR19973065.1212000/150‑1 (MQ=60)

                                                                                                                                       CATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTT              >  SRR19973065.2285285/1‑150 (MQ=60)

                                                                                                                                       CATTTTGTGTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTT              <  SRR19973065.1268697/150‑1 (MQ=60)

                                                                                                                                               GTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA      >  SRR19973065.3211059/1‑150 (MQ=60)

                                                                                                                                               GTTGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATA      >  SRR19973065.3209968/1‑150 (MQ=60)

                                                                                                                                                 TGAGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGT    >  SRR19973065.1457403/1‑150 (MQ=60)

                                                                                                                                                   AGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  SRR19973065.807546/1‑150 (MQ=60)

                                                                                                                                                   AGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  SRR19973065.1442233/1‑150 (MQ=60)

                                                                                                                                                   AGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  SRR19973065.611/1‑150 (MQ=60)

                                                                                                                                                   AGAATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  SRR19973065.3035451/1‑150 (MQ=60)

TTTTAAAATTTCGAATACATGTATTGATCATCTCGAACAATTGATTAACGTCAACTTTTTCTCTTCTGACAGGACGTCATTTTGTGAATGCAATCGTTTTCCATAAATTCTTCTCCCCTCATAGGCGACGAATAGCATTTTGTGTTGAGGATCACAAAACGAATAATTGCTGATCGCCGCGATAAGGTCAGACAAAGACAACAAGGGAAATTTTCACAGAGCTTTTGATCGGCGTAGGCCACAGAATGTTGCTTCGTTTACTGTAACGCCGGGTAAATGAGCGTTTTTTGATAGTGC  >  CP009273/3260852‑3261148

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`