Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,271,128 A→C 100% T145P (ACC→CCC)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,271,1280AC99.6% 1056.7 / ‑5.4 256T145P (ACC→CCC) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base C (164/91);  total (165/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 263 total aligned reads displayed.

CTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCC  >  CP009273/3270983‑3271272
                                                                                                                                                 |                                                                                                                                                
cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAccc                                                                                                                                                <  2:3484463/148‑1 (MQ=255)
cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAccc                                                                                                                                                <  1:729575/148‑1 (MQ=255)
  ttCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCt                                                                                                                                              >  1:299376/1‑148 (MQ=255)
       tataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCAccc                                                                                                                                         >  2:3134657/1‑148 (MQ=255)
          aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCgag                                                                                                                                      >  2:2019233/1‑148 (MQ=255)
             gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGaaa                                                                                                                                   >  1:2564734/1‑148 (MQ=255)
             gAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAAAGTTAAAAACAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGaaa                                                                                                                                   >  2:746763/1‑148 (MQ=255)
              aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAAc                                                                                                                                  <  1:456209/148‑1 (MQ=255)
                    agaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATACTCCTGCCGACTGGGAACCCCTCACCCGAGAAACagaaga                                                                                                                            >  1:2937547/1‑148 (MQ=255)
                     gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAgaagaa                                                                                                                           >  2:2239275/1‑148 (MQ=255)
                     gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAgaagaa                                                                                                                           <  1:925362/148‑1 (MQ=255)
                     gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAgaagaa                                                                                                                           <  2:1755966/148‑1 (MQ=255)
                     gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAgaagaa                                                                                                                           >  2:1737796/1‑148 (MQ=255)
                     gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAgaagaa                                                                                                                           <  1:2677669/148‑1 (MQ=255)
                      aaaGTCATCATTCTGTTATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACTGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCTAGAAACAGAAGaaa                                                                                                                          <  2:3392638/148‑1 (MQ=255)
                       aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAc                                                                                                                         <  2:1057277/148‑1 (MQ=255)
                        aGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAcc                                                                                                                        >  1:247108/1‑148 (MQ=255)
                         gTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAccc                                                                                                                       <  1:2317971/148‑1 (MQ=255)
                           catcatTCTGGGCTGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACGGATGCCTAGACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCAt                                                                                                                     <  2:1378919/148‑1 (MQ=255)
                            atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCAtt                                                                                                                    >  1:941596/1‑148 (MQ=255)
                             tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTg                                                                                                                   >  1:3156874/1‑148 (MQ=255)
                             tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTg                                                                                                                   <  1:1713537/148‑1 (MQ=255)
                              catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                                                                                  >  2:3206133/1‑148 (MQ=255)
                                   tGGGATTGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtg                                                                                                             <  2:2579145/148‑1 (MQ=255)
                                   tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGACGAAACCCATTGATGgtg                                                                                                             <  2:1118306/148‑1 (MQ=255)
                                     ggatggatGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGtt                                                                                                           <  2:3274970/148‑1 (MQ=255)
                                      gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTc                                                                                                          >  2:3091184/1‑148 (MQ=255)
                                      gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTc                                                                                                          <  1:3189833/148‑1 (MQ=255)
                                       atggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTca                                                                                                         >  2:662206/1‑148 (MQ=255)
                                        tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac                                                                                                        >  2:1603599/1‑148 (MQ=255)
                                         ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcaca                                                                                                       <  1:1946240/148‑1 (MQ=255)
                                         ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcaca                                                                                                       <  1:2613852/148‑1 (MQ=255)
                                         ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcaca                                                                                                       >  2:713485/1‑148 (MQ=255)
                                          gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACaa                                                                                                      >  2:2778627/1‑148 (MQ=255)
                                          gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACaa                                                                                                      <  1:845234/148‑1 (MQ=255)
                                             gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCCCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGc                                                                                                   <  2:498976/148‑1 (MQ=255)
                                              aaCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc                                                                                                  <  1:3072081/148‑1 (MQ=255)
                                              aaCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc                                                                                                  <  1:2415357/148‑1 (MQ=255)
                                                    gAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGa                                                                                            <  1:3419415/148‑1 (MQ=255)
                                                         cacTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCa                                                                                       >  2:584315/1‑148 (MQ=255)
                                                           ctctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAtt                                                                                     >  2:2463105/1‑148 (MQ=255)
                                                                cgcACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCgtgt                                                                                <  1:2131367/148‑1 (MQ=255)
                                                                       aCGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAg                                                                         >  1:2542391/1‑148 (MQ=255)
                                                                         ggTAAAAAAACAGCTGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCg                                                                       <  1:2896118/148‑1 (MQ=255)
                                                                          gTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCgg                                                                      >  2:283545/1‑148 (MQ=255)
                                                                           tAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCggg                                                                     >  1:1227042/1‑148 (MQ=255)
                                                                            aaaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGc                                                                    <  2:2754187/148‑1 (MQ=255)
                                                                            aaaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGc                                                                    >  2:952013/1‑148 (MQ=255)
                                                                            aaaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGc                                                                    >  2:1431105/1‑148 (MQ=255)
                                                                             aaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCa                                                                   >  2:1584657/1‑148 (MQ=255)
                                                                              aaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCAt                                                                  >  1:420052/1‑148 (MQ=255)
                                                                               aaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATc                                                                 >  1:2882903/1‑148 (MQ=255)
                                                                                aaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATcc                                                                >  1:1636453/1‑148 (MQ=255)
                                                                                 aaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCt                                                               >  1:1409431/1‑148 (MQ=255)
                                                                                   cAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCttt                                                             >  1:2763287/1‑148 (MQ=255)
                                                                                   cAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCttt                                                             >  2:2980810/1‑148 (MQ=255)
                                                                                     gATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtct                                                           >  1:853800/1‑148 (MQ=255)
                                                                                     gATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTtct                                                           >  1:608507/1‑148 (MQ=255)
                                                                                        gCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAc                                                        >  1:3213753/1‑148 (MQ=255)
                                                                                             taCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTcccc                                                   >  1:1824894/1‑148 (MQ=255)
                                                                                              aCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCg                                                  >  2:1490990/1‑148 (MQ=255)
                                                                                                 gTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGAcc                                               >  2:735250/1‑148 (MQ=255)
                                                                                                  tATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCCTCCTTTCTCACTCCCCGACCa                                              >  2:2224726/1‑148 (MQ=255)
                                                                                                    ttCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGa                                            >  2:84251/1‑148 (MQ=255)
                                                                                                       aGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATc                                         <  2:2754027/148‑1 (MQ=255)
                                                                                                        gCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCa                                        >  2:3233671/1‑148 (MQ=255)
                                                                                                        gCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCa                                        <  1:2857501/148‑1 (MQ=255)
                                                                                                         cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCAc                                       <  1:1094532/148‑1 (MQ=255)
                                                                                                         cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCAc                                       <  1:1774299/148‑1 (MQ=255)
                                                                                                           aaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACtt                                     >  1:820971/1‑148 (MQ=255)
                                                                                                             aTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCa                                   >  1:1362266/1‑148 (MQ=255)
                                                                                                             aTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCa                                   >  1:363467/1‑148 (MQ=255)
                                                                                                             aTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCa                                   >  2:3286553/1‑148 (MQ=255)
                                                                                                              tGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCaa                                  >  2:3052420/1‑148 (MQ=255)
                                                                                                                 tAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAccc                               <  1:696392/148‑1 (MQ=255)
                                                                                                                   aaaaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCa                             >  2:1337568/1‑148 (MQ=255)
                                                                                                                    aaaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAg                            >  2:2529363/1‑148 (MQ=255)
                                                                                                                    aaaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAg                            <  2:362380/148‑1 (MQ=255)
                                                                                                                     aaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGc                           <  2:2329975/148‑1 (MQ=255)
                                                                                                                     aaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGc                           >  2:2862317/1‑148 (MQ=255)
                                                                                                                     aaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGc                           <  2:1674806/148‑1 (MQ=255)
                                                                                                                       gagGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCt                         >  2:2691310/1‑148 (MQ=255)
                                                                                                                       gagGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCt                         >  2:2721613/1‑148 (MQ=255)
                                                                                                                        agGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCtt                        >  2:3057572/1‑148 (MQ=255)
                                                                                                                        agGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCtt                        >  2:3055453/1‑148 (MQ=255)
                                                                                                                        agGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCtt                        >  1:481430/1‑148 (MQ=255)
                                                                                                                        agGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCtt                        <  1:747930/148‑1 (MQ=255)
                                                                                                                             aTCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCa                   >  2:3195543/1‑148 (MQ=255)
                                                                                                                                 tcctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc               <  1:2019233/148‑1 (MQ=255)
                                                                                                                                 tcctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc               <  1:3049008/148‑1 (MQ=255)
                                                                                                                                 tcctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc               <  1:1713817/148‑1 (MQ=255)
                                                                                                                                 tcctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc               <  2:1999803/148‑1 (MQ=255)
                                                                                                                                    tGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc            <  1:1686962/148‑1 (MQ=255)
                                                                                                                                     gCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTcc           >  1:1493736/1‑148 (MQ=255)
                                                                                                                                      ccGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCa          <  1:2239275/148‑1 (MQ=255)
                                                                                                                                          cTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt      >  2:1495977/1‑148 (MQ=255)
                                                                                                                                           tGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTa     >  2:1706972/1‑148 (MQ=255)
                                                                                                                                            gggAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTAt    >  2:1226572/1‑148 (MQ=255)
                                                                                                                                             ggAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATc   >  2:3256600/1‑148 (MQ=255)
                                                                                                                                              gAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATcc  >  1:1542779/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCC  >  CP009273/3270983‑3271272

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAG  >  CP009273/3270979‑3271276
                                                                                                                                                     |                                                                                                                                                    
TCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAC                                                                                                                                                      <  SRR19973065.2522991/150‑1 (MQ=60)
 CTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACC                                                                                                                                                     >  SRR19973065.3271892/1‑150 (MQ=60)
  TTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCC                                                                                                                                                    <  SRR19973065.1489396/150‑1 (MQ=60)
  TTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCC                                                                                                                                                    >  SRR19973065.1994690/1‑150 (MQ=60)
  TTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCC                                                                                                                                                    >  SRR19973065.851435/1‑150 (MQ=60)
    CTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCT                                                                                                                                                  <  SRR19973065.734476/150‑1 (MQ=60)
    CTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCT                                                                                                                                                  >  SRR19973065.301316/1‑150 (MQ=60)
    CTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCT                                                                                                                                                  <  SRR19973065.734460/150‑1 (MQ=60)
      TTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCA                                                                                                                                                >  SRR19973065.2268322/1‑150 (MQ=60)
        CGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACC                                                                                                                                              <  SRR19973065.801862/150‑1 (MQ=60)
            ATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAG                                                                                                                                          <  SRR19973065.2778953/150‑1 (MQ=60)
               GTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAA                                                                                                                                       >  SRR19973065.2580758/1‑150 (MQ=60)
               GTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAA                                                                                                                                       >  SRR19973065.1686011/1‑150 (MQ=60)
               GTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAA                                                                                                                                       >  SRR19973065.1686083/1‑150 (MQ=60)
                  AAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                    <  SRR19973065.459362/150‑1 (MQ=60)
                   AAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGA                                                                                                                                   >  SRR19973065.332050/1‑150 (MQ=60)
                    AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAA                                                                                                                                  >  SRR19973065.649080/1‑150 (MQ=60)
                      AGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGA                                                                                                                                <  SRR19973065.737542/150‑1 (MQ=60)
                      AGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGA                                                                                                                                >  SRR19973065.2955433/1‑150 (MQ=60)
                      AGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGA                                                                                                                                >  SRR19973065.2954944/1‑150 (MQ=60)
                      AGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATACTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGA                                                                                                                                >  SRR19973065.2955364/1‑150 (MQ=60)
                        AGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACATACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATTTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAA                                                                                                                              <  SRR19973065.3112669/150‑1 (MQ=60)
                         GAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAC                                                                                                                             <  SRR19973065.2694271/150‑1 (MQ=60)
                         GAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAC                                                                                                                             <  SRR19973065.931436/150‑1 (MQ=60)
                          AAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACC                                                                                                                            >  SRR19973065.183847/1‑150 (MQ=60)
                          AAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACC                                                                                                                            >  SRR19973065.1891143/1‑150 (MQ=60)
                          AAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACC                                                                                                                            >  SRR19973065.1489047/1‑150 (MQ=60)
                          AAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACC                                                                                                                            >  SRR19973065.248854/1‑150 (MQ=60)
                             GTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCAT                                                                                                                         <  SRR19973065.2332500/150‑1 (MQ=60)
                              TCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATT                                                                                                                        >  SRR19973065.947818/1‑150 (MQ=60)
                              TCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCCCACCCGAGAAACAGAAGAAACCCATT                                                                                                                        >  SRR19973065.502324/1‑150 (MQ=60)
                               CATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTG                                                                                                                       >  SRR19973065.3176016/1‑150 (MQ=60)
                               CATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTG                                                                                                                       <  SRR19973065.2116142/150‑1 (MQ=60)
                                ATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGA                                                                                                                      >  SRR19973065.3024748/1‑150 (MQ=60)
                                 TCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGAT                                                                                                                     <  SRR19973065.1724980/150‑1 (MQ=60)
                                 TCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGAT                                                                                                                     <  SRR19973065.1724931/150‑1 (MQ=60)
                                  CATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATG                                                                                                                    >  SRR19973065.2712798/1‑150 (MQ=60)
                                  CATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATG                                                                                                                    >  SRR19973065.947578/1‑150 (MQ=60)
                                  CATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATG                                                                                                                    >  SRR19973065.947596/1‑150 (MQ=60)
                                          GATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCAC                                                                                                            <  SRR19973065.3209076/150‑1 (MQ=60)
                                          GATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCAC                                                                                                            <  SRR19973065.646645/150‑1 (MQ=60)
                                             GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                         <  SRR19973065.2630052/150‑1 (MQ=60)
                                             GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                         >  SRR19973065.1217948/1‑150 (MQ=60)
                                             GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                         <  SRR19973065.174607/150‑1 (MQ=60)
                                             GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                         <  SRR19973065.174928/150‑1 (MQ=60)
                                             GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                         <  SRR19973065.1958962/150‑1 (MQ=60)
                                              GATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATG                                                                                                        <  SRR19973065.850720/150‑1 (MQ=60)
                                                TGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCC                                                                                                      <  SRR19973065.3217984/150‑1 (MQ=60)
                                                TGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCC                                                                                                      <  SRR19973065.2923059/150‑1 (MQ=60)
                                                 GAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCC                                                                                                     <  SRR19973065.1344880/150‑1 (MQ=60)
                                                  AACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCG                                                                                                    <  SRR19973065.2430573/150‑1 (MQ=60)
                                                  AACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCG                                                                                                    <  SRR19973065.3090857/150‑1 (MQ=60)
                                                     GATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCG                                                                                                 >  SRR19973065.2164547/1‑150 (MQ=60)
                                                        GAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAC                                                                                              <  SRR19973065.3440305/150‑1 (MQ=60)
                                                         ANAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACG                                                                                             >  SRR19973065.3291642/1‑150 (MQ=60)
                                                            ACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAT                                                                                          >  SRR19973065.2629791/1‑150 (MQ=60)
                                                             CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                         >  SRR19973065.2281090/1‑150 (MQ=60)
                                                             CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                         >  SRR19973065.2854213/1‑150 (MQ=60)
                                                               CTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCG                                                                                       >  SRR19973065.364287/1‑150 (MQ=60)
                                                               CTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCG                                                                                       >  SRR19973065.248147/1‑150 (MQ=60)
                                                                    CGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTG                                                                                  <  SRR19973065.3013956/150‑1 (MQ=60)
                                                                    CGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTG                                                                                  <  SRR19973065.2145080/150‑1 (MQ=60)
                                                                      CACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAC                                                                                >  SRR19973065.2443229/1‑150 (MQ=60)
                                                                      CACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAC                                                                                >  SRR19973065.2570874/1‑150 (MQ=60)
                                                                        CCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCA                                                                              >  SRR19973065.2568191/1‑150 (MQ=60)
                                                                         CTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAG                                                                             >  SRR19973065.366709/1‑150 (MQ=60)
                                                                         CTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAG                                                                             >  SRR19973065.2880224/1‑150 (MQ=60)
                                                                         CTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAG                                                                             >  SRR19973065.2558314/1‑150 (MQ=60)
                                                                          TACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGC                                                                            <  SRR19973065.1527609/150‑1 (MQ=60)
                                                                          TACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGC                                                                            <  SRR19973065.1527651/150‑1 (MQ=60)
                                                                          TACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGC                                                                            >  SRR19973065.777709/1‑150 (MQ=60)
                                                                          TACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGC                                                                            >  SRR19973065.2548134/1‑150 (MQ=60)
                                                                           ACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCG                                                                           <  SRR19973065.1611989/150‑1 (MQ=60)
                                                                             GGTAAAAAAACAGCTGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                         <  SRR19973065.2913799/150‑1 (MQ=60)
                                                                             GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                         <  SRR19973065.2913418/150‑1 (MQ=60)
                                                                             GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                         >  SRR19973065.1235052/1‑150 (MQ=60)
                                                                                AAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCAT                                                                      >  SRR19973065.2201585/1‑150 (MQ=60)
                                                                                AAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCAT                                                                      >  SRR19973065.422982/1‑150 (MQ=60)
                                                                                 AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATC                                                                     >  SRR19973065.2900560/1‑150 (MQ=60)
                                                                                 AAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATC                                                                     >  SRR19973065.2900543/1‑150 (MQ=60)
                                                                                  AAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCC                                                                    >  SRR19973065.1647442/1‑150 (MQ=60)
                                                                                   AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCT                                                                   >  SRR19973065.1723663/1‑150 (MQ=60)
                                                                                   AAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCT                                                                   >  SRR19973065.1418651/1‑150 (MQ=60)
                                                                                     AACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTT                                                                 >  SRR19973065.2780457/1‑150 (MQ=60)
                                                                                       CAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT                                                               >  SRR19973065.3533375/1‑150 (MQ=60)
                                                                                       CAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT                                                               >  SRR19973065.859322/1‑150 (MQ=60)
                                                                                       CAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT                                                               >  SRR19973065.2627259/1‑150 (MQ=60)
                                                                                       CAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCT                                                               >  SRR19973065.612375/1‑150 (MQ=60)
                                                                                          ATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAC                                                            >  SRR19973065.3233085/1‑150 (MQ=60)
                                                                                             CCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCC                                                         >  SRR19973065.137992/1‑150 (MQ=60)
                                                                                              CTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCC                                                        >  SRR19973065.828898/1‑150 (MQ=60)
                                                                                              CTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCC                                                        >  SRR19973065.480194/1‑150 (MQ=60)
                                                                                               TATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCC                                                       >  SRR19973065.1836910/1‑150 (MQ=60)
                                                                                               TATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCC                                                       >  SRR19973065.2356383/1‑150 (MQ=60)
                                                                                                ATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCG                                                      >  SRR19973065.539909/1‑150 (MQ=60)
                                                                                                    CGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCA                                                  <  SRR19973065.431356/150‑1 (MQ=60)
                                                                                                        TTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAAT                                              <  SRR19973065.1883922/150‑1 (MQ=60)
                                                                                                            GCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACT                                          <  SRR19973065.2875054/150‑1 (MQ=60)
                                                                                                            GCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACT                                          <  SRR19973065.87856/150‑1 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         >  SRR19973065.826363/1‑150 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         <  SRR19973065.1101737/150‑1 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         >  SRR19973065.1980116/1‑150 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         <  SRR19973065.2863891/150‑1 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         <  SRR19973065.1785907/150‑1 (MQ=60)
                                                                                                             CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                         >  SRR19973065.3068118/1‑150 (MQ=60)
                                                                                                               AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                       >  SRR19973065.366010/1‑150 (MQ=60)
                                                                                                               AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                       >  SRR19973065.1371286/1‑150 (MQ=60)
                                                                                                                     TAAAAAGAGGACATCCTCCTGCCGACTGTGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACTCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCA                                 <  SRR19973065.3130388/150‑1 (MQ=60)
                                                                                                                     TAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCA                                 <  SRR19973065.701024/150‑1 (MQ=60)
                                                                                                                     TAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCA                                 <  SRR19973065.731207/150‑1 (MQ=60)
                                                                                                                        AAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCC                              >  SRR19973065.852891/1‑150 (MQ=60)
                                                                                                                        AAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCC                              >  SRR19973065.3183407/1‑150 (MQ=60)
                                                                                                                          AGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTT                            >  SRR19973065.484707/1‑150 (MQ=60)
                                                                                                                            AGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTC                          <  SRR19973065.752932/150‑1 (MQ=60)
                                                                                                                            AGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTC                          >  SRR19973065.43566/1‑150 (MQ=60)
                                                                                                                             GGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCG                         >  SRR19973065.3197495/1‑150 (MQ=60)
                                                                                                                               ACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCA                       >  SRR19973065.1437900/1‑150 (MQ=60)
                                                                                                                                CATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCTCAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAG                      <  SRR19973065.2633981/150‑1 (MQ=60)
                                                                                                                                 ATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGT                     <  SRR19973065.1708443/150‑1 (MQ=60)
                                                                                                                                     TCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTT                 <  SRR19973065.1725211/150‑1 (MQ=60)
                                                                                                                                     TCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTT                 <  SRR19973065.3067660/150‑1 (MQ=60)
                                                                                                                                     TCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTT                 <  SRR19973065.2032230/150‑1 (MQ=60)
                                                                                                                                     TCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTT                 <  SRR19973065.128547/150‑1 (MQ=60)
                                                                                                                                       CTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCC               >  SRR19973065.1503330/1‑150 (MQ=60)
                                                                                                                                        TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA              <  SRR19973065.1698234/150‑1 (MQ=60)
                                                                                                                                          CCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGG            <  SRR19973065.2253511/150‑1 (MQ=60)
                                                                                                                                                GGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCC      >  SRR19973065.1552979/1‑150 (MQ=60)
                                                                                                                                                    ACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTTACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAG  >  SRR19973065.3382658/1‑150 (MQ=60)
                                                                                                                                                    ACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTTACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAG  >  SRR19973065.3387266/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
TCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAG  >  CP009273/3270979‑3271276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑