Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,271,128 A→C 86.8% T145P (ACC→CCC)  yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,271,1280AC86.8% 987.6 / 80.0 297T145P (ACC→CCC) yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (31/9);  new base C (138/119);  total (169/128)
Fisher's exact test for biased strand distribution p-value = 5.59e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 298 total aligned reads displayed.

TTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCG  >  CP009273/3270981‑3271273
                                                                                                                                                   |                                                                                                                                                 
    ttCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTc                                                                                                                                              >  2:3054398/1‑149 (MQ=255)
        ttATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCAccc                                                                                                                                          >  2:3510151/1‑149 (MQ=255)
        ttATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCAccc                                                                                                                                          <  1:2734424/149‑1 (MQ=255)
         tataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCg                                                                                                                                         >  1:2170296/1‑149 (MQ=255)
         tataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCg                                                                                                                                         <  1:3639185/149‑1 (MQ=255)
          ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCga                                                                                                                                        >  1:3430476/1‑149 (MQ=255)
           taGTGAAAAAGAGAAAGTCATCAGTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCgag                                                                                                                                       <  1:4016191/149‑1 (MQ=255)
             gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGaa                                                                                                                                     >  1:2595309/1‑149 (MQ=255)
              tGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGaaa                                                                                                                                    >  1:244383/1‑149 (MQ=255)
                aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACa                                                                                                                                  <  1:4672173/149‑1 (MQ=255)
                aaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa                                                                                                                                  <  2:2577809/149‑1 (MQ=255)
                 aaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACag                                                                                                                                 >  1:3940514/1‑149 (MQ=255)
                  aaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACaga                                                                                                                                >  2:3688052/1‑149 (MQ=255)
                   aaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACagaa                                                                                                                               >  1:4042080/1‑149 (MQ=255)
                   aaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACagaa                                                                                                                               >  1:2095051/1‑149 (MQ=255)
                    agagAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACagaag                                                                                                                              >  2:3828889/1‑149 (MQ=255)
                     gagaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACagaaga                                                                                                                             >  2:1868265/1‑149 (MQ=255)
                         aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAcc                                                                                                                         <  2:4728860/149‑1 (MQ=255)
                         aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAcc                                                                                                                         <  1:4570385/149‑1 (MQ=255)
                         aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAAcc                                                                                                                         <  2:4103470/149‑1 (MQ=255)
                            tcatcaTTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCAt                                                                                                                      <  2:1425638/149‑1 (MQ=255)
                               tcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGa                                                                                                                   <  2:1935218/149‑1 (MQ=255)
                                catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGAt                                                                                                                  <  1:10156/149‑1 (MQ=255)
                                  ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                                                                                >  2:1508096/1‑149 (MQ=255)
                                  ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                                                                                <  2:3130511/149‑1 (MQ=255)
                                  ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                                                                                <  2:2712028/149‑1 (MQ=255)
                                  ttCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATgg                                                                                                                <  2:2960876/149‑1 (MQ=255)
                                   tCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGgt                                                                                                               <  1:70253/149‑1 (MQ=255)
                                     tGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGgtgt                                                                                                             <  2:760743/149‑1 (MQ=255)
                                         atggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTcac                                                                                                         <  2:528969/149‑1 (MQ=255)
                                            gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                                                                                                      <  2:1461309/149‑1 (MQ=255)
                                            gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAt                                                                                                      >  1:4561863/1‑149 (MQ=255)
                                              tgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGc                                                                                                    >  1:4714801/1‑149 (MQ=255)
                                               gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc                                                                                                   <  2:287981/149‑1 (MQ=255)
                                               gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGcc                                                                                                   <  1:2510153/149‑1 (MQ=255)
                                                aaCGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGccc                                                                                                  <  2:1639006/149‑1 (MQ=255)
                                                  cGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgc                                                                                                <  2:3859530/149‑1 (MQ=255)
                                                  cGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCccgc                                                                                                >  1:1237263/1‑149 (MQ=255)
                                                       aaaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc                                                                                           >  2:3422009/1‑149 (MQ=255)
                                                       aaaaCACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAc                                                                                           >  2:3763608/1‑149 (MQ=255)
                                                          acacTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCa                                                                                        >  2:1361053/1‑149 (MQ=255)
                                                           cacTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                                                                       <  1:2677191/149‑1 (MQ=255)
                                                           cacTCTGCGCACATACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAt                                                                                       <  2:334373/149‑1 (MQ=255)
                                                            acTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAtt                                                                                      <  2:3171795/149‑1 (MQ=255)
                                                              tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCg                                                                                    >  1:766662/1‑149 (MQ=255)
                                                              tctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCg                                                                                    >  1:1762882/1‑149 (MQ=255)
                                                               ctGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAACAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCgt                                                                                   <  2:3770426/149‑1 (MQ=255)
                                                                    cACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACACTGCCCGCCGAACGCATTCGTGTTGa                                                                              >  2:2459416/1‑149 (MQ=255)
                                                                     aCCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGAc                                                                             <  1:1567807/149‑1 (MQ=255)
                                                                       cTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCa                                                                           >  2:2665644/1‑149 (MQ=255)
                                                                         aCGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGc                                                                         <  2:3940541/149‑1 (MQ=255)
                                                                           ggTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACGGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCgg                                                                       >  2:2122121/1‑149 (MQ=255)
                                                                           ggTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCgg                                                                       <  1:4570917/149‑1 (MQ=255)
                                                                           ggTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCgg                                                                       >  1:590407/1‑149 (MQ=255)
                                                                               aaaaaaCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCAt                                                                   >  1:2930871/1‑149 (MQ=255)
                                                                                    aCAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCttt                                                              >  1:3521979/1‑149 (MQ=255)
                                                                                         tGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCAc                                                         >  2:607389/1‑149 (MQ=255)
                                                                                              ataCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTcccc                                                    <  1:2805467/149‑1 (MQ=255)
                                                                                              ataCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTcccc                                                    >  2:3316802/1‑149 (MQ=255)
                                                                                                aCCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGa                                                  <  2:1974999/149‑1 (MQ=255)
                                                                                                  cGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGAcc                                                <  1:4328220/149‑1 (MQ=255)
                                                                                                    tATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAg                                              >  1:8576/1‑149 (MQ=255)
                                                                                                     aTTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGa                                             <  2:4587369/149‑1 (MQ=255)
                                                                                                     aTTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGa                                             >  2:3691541/1‑149 (MQ=255)
                                                                                                     aTTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGa                                             >  2:3391810/1‑149 (MQ=255)
                                                                                                       tCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAAt                                           >  1:1075692/1‑149 (MQ=255)
                                                                                                         aGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCa                                         >  1:3328335/1‑149 (MQ=255)
                                                                                                          gCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCAc                                        >  2:4358438/1‑149 (MQ=255)
                                                                                                           cAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACt                                       <  1:4445324/149‑1 (MQ=255)
                                                                                                            aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACtt                                      >  2:866317/1‑149 (MQ=255)
                                                                                                            aaaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACtt                                      >  1:4512555/1‑149 (MQ=255)
                                                                                                             aaaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTc                                     <  2:1010251/149‑1 (MQ=255)
                                                                                                              aaTGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCa                                    >  1:376856/1‑149 (MQ=255)
                                                                                                                 gTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAcc                                 >  2:4759915/1‑149 (MQ=255)
                                                                                                                 gTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAAcc                                 >  2:4322506/1‑149 (MQ=255)
                                                                                                                 gTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACATCAAcc                                 >  2:2220179/1‑149 (MQ=255)
                                                                                                                    aaaaaGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCa                              >  2:2245621/1‑149 (MQ=255)
                                                                                                                      aaaGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGc                            >  2:1205086/1‑149 (MQ=255)
                                                                                                                        agagGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCt                          >  2:685430/1‑149 (MQ=255)
                                                                                                                         gagGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCtt                         <  1:2325882/149‑1 (MQ=255)
                                                                                                                            gACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCg                      <  1:3071790/149‑1 (MQ=255)
                                                                                                                            gACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTGTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCg                      >  1:1975149/1‑149 (MQ=255)
                                                                                                                             aCATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGc                     >  1:2950931/1‑149 (MQ=255)
                                                                                                                              cATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCa                    <  1:3595302/149‑1 (MQ=255)
                                                                                                                              cATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCa                    >  2:1327695/1‑149 (MQ=255)
                                                                                                                              cATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCa                    >  2:1378356/1‑149 (MQ=255)
                                                                                                                                  ctcctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTcc                >  1:4207359/1‑149 (MQ=255)
                                                                                                                                     ctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc             >  1:4775440/1‑149 (MQ=255)
                                                                                                                                     ctGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTc             >  1:3220898/1‑149 (MQ=255)
                                                                                                                                      tGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTcc            >  2:3621247/1‑149 (MQ=255)
                                                                                                                                      tGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTcc            <  2:536953/149‑1 (MQ=255)
                                                                                                                                      tGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTcc            <  2:2170291/149‑1 (MQ=255)
                                                                                                                                       gCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCa           >  2:1773913/1‑149 (MQ=255)
                                                                                                                                          gACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGc        <  1:3233087/149‑1 (MQ=255)
                                                                                                                                          gACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGc        <  1:1788691/149‑1 (MQ=255)
                                                                                                                                           aCTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCt       <  2:4237701/149‑1 (MQ=255)
                                                                                                                                            cTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTa      >  2:2685313/1‑149 (MQ=255)
                                                                                                                                             tGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTAt     <  1:1508096/149‑1 (MQ=255)
                                                                                                                                              gggAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATc    >  2:4482082/1‑149 (MQ=255)
                                                                                                                                               ggAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATcc   <  2:1096819/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
TTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCG  >  CP009273/3270981‑3271273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGG  >  CP009273/3270980‑3271277
                                                                                                                                                    |                                                                                                                                                     
CTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACC                                                                                                                                                      >  SRR19973071.1352094/1‑150 (MQ=60)
CTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAC                                                                                                                                                      >  SRR19973071.2232390/1‑150 (MQ=60)
  TCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCC                                                                                                                                                    <  SRR19973071.1782123/150‑1 (MQ=60)
    TTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTC                                                                                                                                                  >  SRR19973071.4515654/1‑150 (MQ=60)
    TTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTC                                                                                                                                                  >  SRR19973071.3130229/1‑150 (MQ=60)
    TTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTC                                                                                                                                                  >  SRR19973071.4515394/1‑150 (MQ=60)
        GTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCC                                                                                                                                              <  SRR19973071.2530214/150‑1 (MQ=60)
        GTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCC                                                                                                                                              >  SRR19973071.4207114/1‑150 (MQ=60)
         TTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCG                                                                                                                                             <  SRR19973071.2732082/150‑1 (MQ=60)
         TTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCG                                                                                                                                             <  SRR19973071.2744133/150‑1 (MQ=60)
         TTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCG                                                                                                                                             <  SRR19973071.2744191/150‑1 (MQ=60)
         TTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCG                                                                                                                                             >  SRR19973071.2178328/1‑150 (MQ=60)
         TTATAGTAAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCG                                                                                                                                             >  SRR19973071.2178323/1‑150 (MQ=60)
          TATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGA                                                                                                                                            >  SRR19973071.3442605/1‑150 (MQ=60)
          TATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGA                                                                                                                                            <  SRR19973071.3651979/150‑1 (MQ=60)
           ATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAG                                                                                                                                           <  SRR19973071.2665037/150‑1 (MQ=60)
           ATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAG                                                                                                                                           <  SRR19973071.1139054/150‑1 (MQ=60)
           ATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAG                                                                                                                                           >  SRR19973071.2025473/1‑150 (MQ=60)
           ATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAG                                                                                                                                           >  SRR19973071.4550072/1‑150 (MQ=60)
            TAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGA                                                                                                                                          <  SRR19973071.4030405/150‑1 (MQ=60)
            TAGTGAAAAAGAGAAAGTCATCAGTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGA                                                                                                                                          <  SRR19973071.4030294/150‑1 (MQ=60)
             AGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAA                                                                                                                                         >  SRR19973071.2604707/1‑150 (MQ=60)
              GTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAA                                                                                                                                        >  SRR19973071.245316/1‑150 (MQ=60)
               TGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAAC                                                                                                                                       <  SRR19973071.538903/150‑1 (MQ=60)
                 AAAAATAGAAATTCATCATTCTGGGATGGATGAACGATGAAAAAACTCTGCGAACATACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                     <  SRR19973071.4271572/150‑1 (MQ=60)
                 AAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                     <  SRR19973071.842935/150‑1 (MQ=60)
                 AAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                     <  SRR19973071.4688808/150‑1 (MQ=60)
                 AAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                     >  SRR19973071.3954368/1‑150 (MQ=60)
                 AAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAG                                                                                                                                     >  SRR19973071.3954395/1‑150 (MQ=60)
                  AAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGA                                                                                                                                    >  SRR19973071.1778994/1‑150 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAA                                                                                                                                   >  SRR19973071.2963924/1‑150 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAA                                                                                                                                   >  SRR19973071.2102773/1‑150 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAA                                                                                                                                   <  SRR19973071.3960627/150‑1 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAA                                                                                                                                   >  SRR19973071.4056346/1‑150 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAA                                                                                                                                   >  SRR19973071.3240073/1‑150 (MQ=60)
                   AAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAA                                                                                                                                   >  SRR19973071.2855911/1‑150 (MQ=60)
                     AGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGA                                                                                                                                 >  SRR19973071.1127525/1‑150 (MQ=60)
                      GAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGACCCCCTCACCCGAGAAACAGAAGAA                                                                                                                                >  SRR19973071.4585338/1‑150 (MQ=60)
                         AAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACC                                                                                                                             >  SRR19973071.2369163/1‑150 (MQ=60)
                          AAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCC                                                                                                                            <  SRR19973071.4586642/150‑1 (MQ=60)
                          AAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCC                                                                                                                            >  SRR19973071.3914478/1‑150 (MQ=60)
                          AAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCC                                                                                                                            <  SRR19973071.4586671/150‑1 (MQ=60)
                             TCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATT                                                                                                                         <  SRR19973071.3263360/150‑1 (MQ=60)
                              CATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTG                                                                                                                        >  SRR19973071.1698480/1‑150 (MQ=60)
                               ATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGA                                                                                                                       <  SRR19973071.2512974/150‑1 (MQ=60)
                               ATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA                                                                                                                       >  SRR19973071.3628408/1‑150 (MQ=60)
                                 CATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATG                                                                                                                     <  SRR19973071.10230/150‑1 (MQ=60)
                                  ATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGG                                                                                                                    >  SRR19973071.2187623/1‑150 (MQ=60)
                                    TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTG                                                                                                                  <  SRR19973071.70629/150‑1 (MQ=60)
                                        GGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCA                                                                                                              <  SRR19973071.2574597/150‑1 (MQ=60)
                                        GGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCA                                                                                                              <  SRR19973071.3508441/150‑1 (MQ=60)
                                            GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                          >  SRR19973071.414172/1‑150 (MQ=60)
                                            GGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAT                                                                                                          >  SRR19973071.4578114/1‑150 (MQ=60)
                                              ATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGC                                                                                                        >  SRR19973071.3431625/1‑150 (MQ=60)
                                              ATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGC                                                                                                        >  SRR19973071.4731516/1‑150 (MQ=60)
                                                GAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCC                                                                                                      <  SRR19973071.2519290/150‑1 (MQ=60)
                                                  ACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGC                                                                                                    >  SRR19973071.1241648/1‑150 (MQ=60)
                                                    GATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCG                                                                                                  <  SRR19973071.2461700/150‑1 (MQ=60)
                                                       GAAAACACTCTGCGCACCTACTGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAC                                                                                               <  SRR19973071.3350185/150‑1 (MQ=60)
                                                       GAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAAC                                                                                               >  SRR19973071.2030038/1‑150 (MQ=60)
                                                         AAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGC                                                                                             >  SRR19973071.1558581/1‑150 (MQ=60)
                                                           ACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCAT                                                                                           <  SRR19973071.651276/150‑1 (MQ=60)
                                                            CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                          <  SRR19973071.2686773/150‑1 (MQ=60)
                                                            CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                          <  SRR19973071.3672323/150‑1 (MQ=60)
                                                            CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                          <  SRR19973071.2686554/150‑1 (MQ=60)
                                                            CACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATT                                                                                          <  SRR19973071.2686827/150‑1 (MQ=60)
                                                              CTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCG                                                                                        >  SRR19973071.3461460/1‑150 (MQ=60)
                                                              CTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCG                                                                                        >  SRR19973071.769261/1‑150 (MQ=60)
                                                              CTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCG                                                                                        >  SRR19973071.1769463/1‑150 (MQ=60)
                                                                CTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTG                                                                                      <  SRR19973071.1399823/150‑1 (MQ=60)
                                                                 TGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGT                                                                                     >  SRR19973071.1696657/1‑150 (MQ=60)
                                                                 TGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGT                                                                                     <  SRR19973071.570102/150‑1 (MQ=60)
                                                                  GCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTT                                                                                    <  SRR19973071.4758518/150‑1 (MQ=60)
                                                                    GCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAAGGCCCGCCGAACGCATTCGTGTTGA                                                                                  <  SRR19973071.357072/150‑1 (MQ=60)
                                                                      ACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACC                                                                                <  SRR19973071.1573373/150‑1 (MQ=60)
                                                                          ACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCG                                                                            <  SRR19973071.4679112/150‑1 (MQ=60)
                                                                           CGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGG                                                                           <  SRR19973071.4701268/150‑1 (MQ=60)
                                                                           CGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGG                                                                           >  SRR19973071.592608/1‑150 (MQ=60)
                                                                            GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                          <  SRR19973071.702426/150‑1 (MQ=60)
                                                                            GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                          <  SRR19973071.4586730/150‑1 (MQ=60)
                                                                            GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                          <  SRR19973071.4587205/150‑1 (MQ=60)
                                                                            GGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGG                                                                          <  SRR19973071.4586774/150‑1 (MQ=60)
                                                                             GTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGC                                                                         >  SRR19973071.3552110/1‑150 (MQ=60)
                                                                               AAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCAT                                                                       >  SRR19973071.2941372/1‑150 (MQ=60)
                                                                                   AAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGACCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTT                                                                   >  SRR19973071.831617/1‑150 (MQ=60)
                                                                                    AACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTT                                                                  >  SRR19973071.2773990/1‑150 (MQ=60)
                                                                                    AACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTT                                                                  >  SRR19973071.3534320/1‑150 (MQ=60)
                                                                                       AGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTC                                                               <  SRR19973071.4296779/150‑1 (MQ=60)
                                                                                               ATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCG                                                       >  SRR19973071.1049775/1‑150 (MQ=60)
                                                                                               ATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCG                                                       <  SRR19973071.2815345/150‑1 (MQ=60)
                                                                                                   CGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCA                                                   <  SRR19973071.4343499/150‑1 (MQ=60)
                                                                                                    GTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAG                                                  >  SRR19973071.8643/1‑150 (MQ=60)
                                                                                                      ATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAA                                                >  SRR19973071.4053394/1‑150 (MQ=60)
                                                                                                      ATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAA                                                >  SRR19973071.4765134/1‑150 (MQ=60)
                                                                                                       TTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAAT                                               <  SRR19973071.3195347/150‑1 (MQ=60)
                                                                                                       TTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAAT                                               >  SRR19973071.1079518/1‑150 (MQ=60)
                                                                                                         CAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCA                                             <  SRR19973071.864183/150‑1 (MQ=60)
                                                                                                         CAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCA                                             >  SRR19973071.3340131/1‑150 (MQ=60)
                                                                                                          AGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCAC                                            >  SRR19973071.4711064/1‑150 (MQ=60)
                                                                                                           GCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACT                                           <  SRR19973071.2881808/150‑1 (MQ=60)
                                                                                                            CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                          >  SRR19973071.4528609/1‑150 (MQ=60)
                                                                                                            CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                          <  SRR19973071.4461038/150‑1 (MQ=60)
                                                                                                            CAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTT                                          <  SRR19973071.4460808/150‑1 (MQ=60)
                                                                                                             AAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTC                                         <  SRR19973071.3842461/150‑1 (MQ=60)
                                                                                                              AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                        >  SRR19973071.751115/1‑150 (MQ=60)
                                                                                                              AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                        >  SRR19973071.751204/1‑150 (MQ=60)
                                                                                                              AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                        <  SRR19973071.869412/150‑1 (MQ=60)
                                                                                                              AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                        >  SRR19973071.378045/1‑150 (MQ=60)
                                                                                                              AAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCA                                        >  SRR19973071.377364/1‑150 (MQ=60)
                                                                                                                 TGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACC                                     >  SRR19973071.4395285/1‑150 (MQ=60)
                                                                                                                    TAAAAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCA                                  <  SRR19973071.3246421/150‑1 (MQ=60)
                                                                                                                       AAAGAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCC                               >  SRR19973071.3631905/1‑150 (MQ=60)
                                                                                                                          GAGGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTT                            <  SRR19973071.2334343/150‑1 (MQ=60)
                                                                                                                            GGACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTGTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCG                          >  SRR19973071.1982303/1‑150 (MQ=60)
                                                                                                                             GACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGC                         >  SRR19973071.2961563/1‑150 (MQ=60)
                                                                                                                             GACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGC                         <  SRR19973071.3082671/150‑1 (MQ=60)
                                                                                                                             GACATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGC                         >  SRR19973071.2961499/1‑150 (MQ=60)
                                                                                                                               CATCCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAG                       <  SRR19973071.3607792/150‑1 (MQ=60)
                                                                                                                                  CCTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCC                    >  SRR19973071.4222329/1‑150 (MQ=60)
                                                                                                                                   CTCCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCT                   >  SRR19973071.2129588/1‑150 (MQ=60)
                                                                                                                                     CCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTC                 >  SRR19973071.3232207/1‑150 (MQ=60)
                                                                                                                                     CCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTC                 >  SRR19973071.3232373/1‑150 (MQ=60)
                                                                                                                                     CCTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTC                 >  SRR19973071.4792290/1‑150 (MQ=60)
                                                                                                                                      CTGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCC                >  SRR19973071.1552435/1‑150 (MQ=60)
                                                                                                                                       TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA               <  SRR19973071.2958960/150‑1 (MQ=60)
                                                                                                                                       TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA               >  SRR19973071.4561036/1‑150 (MQ=60)
                                                                                                                                       TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA               <  SRR19973071.2427456/150‑1 (MQ=60)
                                                                                                                                       TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA               >  SRR19973071.4590294/1‑150 (MQ=60)
                                                                                                                                       TGCCGACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCA               <  SRR19973071.2958990/150‑1 (MQ=60)
                                                                                                                                           GACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCT           <  SRR19973071.1795359/150‑1 (MQ=60)
                                                                                                                                           GACTGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCT           <  SRR19973071.3244461/150‑1 (MQ=60)
                                                                                                                                              TGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATC        <  SRR19973071.1513231/150‑1 (MQ=60)
                                                                                                                                              TGGGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATC        <  SRR19973071.1513545/150‑1 (MQ=60)
                                                                                                                                                GGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCG      >  SRR19973071.1847555/1‑150 (MQ=60)
                                                                                                                                                GGAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCG      >  SRR19973071.1847582/1‑150 (MQ=60)
                                                                                                                                                 GAACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGC     >  SRR19973071.1515604/1‑150 (MQ=60)
                                                                                                                                                   ACCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAG   <  SRR19973071.1271996/150‑1 (MQ=60)
                                                                                                                                                    CCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGG  <  SRR19973071.2614662/150‑1 (MQ=60)
                                                                                                                                                    CCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGG  <  SRR19973071.1858394/150‑1 (MQ=60)
                                                                                                                                                    CCCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGG  <  SRR19973071.2614578/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGATGGTGTTCACAATGCCCGCCGAACGCATTCGTGTTGACCAGCGGGCATCCTTTCTCACTCCCCGACCAGAATCACTTCAACCCCAGCCTTTCGCAGTCCTTCCAGGCTATCCGCAGG  >  CP009273/3270980‑3271277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑