Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,392,190 G→T 43.2% V485V (GTC→GTA)  nuoG ← NADH:ubiquinone oxidoreductase, chain G

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,392,1900GT43.2% 280.1 / inf 284V485V (GTC→GTA) nuoGNADH:ubiquinone oxidoreductase, chain G
Reads supporting (aligned to +/- strand):  ref base G (85/76);  new base T (65/57);  total (151/133)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 288 total aligned reads displayed.

AACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTC  >  CP009273/2392042‑2392339
                                                                                                                                                    |                                                                                                                                                     
 aCGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGAc                                                                                                                                                     <  2:891049/150‑1 (MQ=255)
  cGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACg                                                                                                                                                    >  2:335572/1‑150 (MQ=255)
    ggCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTc                                                                                                                                                  <  2:191627/150‑1 (MQ=255)
         tCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCAGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGAttt                                                                                                                                             <  1:699741/150‑1 (MQ=255)
          cATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGAtttt                                                                                                                                            >  2:3778920/1‑150 (MQ=255)
           aTGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttg                                                                                                                                           >  1:342392/1‑150 (MQ=255)
             ggTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATtttgct                                                                                                                                         >  1:2410587/1‑150 (MQ=255)
              gTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgctt                                                                                                                                        >  1:99837/1‑150 (MQ=255)
               tGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATtttgcttt                                                                                                                                       >  2:3898970/1‑150 (MQ=255)
                gATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATtttgctttg                                                                                                                                      >  2:30351/1‑150 (MQ=255)
                  tACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCa                                                                                                                                    >  1:2673106/1‑150 (MQ=255)
                       aCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCa                                                                                                                               <  1:3875550/150‑1 (MQ=255)
                       aCGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCa                                                                                                                               <  2:1061261/150‑1 (MQ=255)
                           cAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGtt                                                                                                                           >  1:1497493/1‑150 (MQ=255)
                           cAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGtt                                                                                                                           >  1:1804325/1‑150 (MQ=255)
                              cgccgcGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGa                                                                                                                        >  2:2439615/1‑150 (MQ=255)
                               gccgcGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGAt                                                                                                                       >  1:3249026/1‑150 (MQ=255)
                                ccgcGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATa                                                                                                                      <  2:1859614/150‑1 (MQ=255)
                                   cgACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCg                                                                                                                   <  2:4618294/150‑1 (MQ=255)
                                    gACCTGTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGt                                                                                                                  <  2:2480920/150‑1 (MQ=255)
                                      ccTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCa                                                                                                                <  2:1813084/150‑1 (MQ=255)
                                       cTTTCAGGGCTTTCGCGACGTGAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCaa                                                                                                               <  2:152471/150‑1 (MQ=255)
                                        tttCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAAc                                                                                                              >  2:4046265/1‑150 (MQ=255)
                                        tttCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAAc                                                                                                              >  1:3633550/1‑150 (MQ=255)
                                          tCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCg                                                                                                            <  1:1957665/150‑1 (MQ=255)
                                             gggCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTg                                                                                                         <  2:4142672/150‑1 (MQ=255)
                                             gggCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTg                                                                                                         <  2:591640/150‑1 (MQ=255)
                                               gCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGt                                                                                                       >  2:1044130/1‑150 (MQ=255)
                                                cTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTg                                                                                                      <  1:4185747/150‑1 (MQ=255)
                                                 tttCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGc                                                                                                     >  1:1649406/1‑150 (MQ=255)
                                                  ttCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCa                                                                                                    <  1:238864/150‑1 (MQ=255)
                                                       gACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGtt                                                                                               >  2:2193251/1‑150 (MQ=255)
                                                       gACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGtt                                                                                               >  1:1359640/1‑150 (MQ=255)
                                                         cGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTAt                                                                                             >  2:4311560/1‑150 (MQ=255)
                                                          gTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATc                                                                                            <  2:2699634/150‑1 (MQ=255)
                                                          gTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATc                                                                                            >  2:1115277/1‑150 (MQ=255)
                                                                  ccgccTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCAt                                                                                    >  2:3249536/1‑150 (MQ=255)
                                                                    gccTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATgg                                                                                  <  2:3170753/150‑1 (MQ=255)
                                                                     ccTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATggg                                                                                 <  1:2927165/150‑1 (MQ=255)
                                                                       tGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCg                                                                               <  2:1547787/150‑1 (MQ=255)
                                                                        gAATCACCTCTAAGCTACACGCGTACGTCCCTGAGAAAATCAACGGTTTATTAGCACCTGCAAGTCCCTGCACGAAGACGTCGATTATGCTATGCAGCTCAGGTTCGATACCGACAACCGCTGATGCAGAGTTATACAGCGCAAGGGCGa                                                                              >  2:16595/1‑150 (MQ=255)
                                                                          aTCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATg                                                                            <  1:2036161/150‑1 (MQ=255)
                                                                           tCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATgg                                                                           <  2:824212/150‑1 (MQ=255)
                                                                              ccTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCaa                                                                        <  2:4519820/150‑1 (MQ=255)
                                                                                 ctAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAAc                                                                     <  1:4307913/150‑1 (MQ=255)
                                                                                  tAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAAcc                                                                    >  1:4368163/1‑150 (MQ=255)
                                                                                  tAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAAcc                                                                    <  2:693432/150‑1 (MQ=255)
                                                                                    aGCTACCGGCGTTCGTCCCGGAGATAATCCACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTa                                                                  >  1:2321456/1‑150 (MQ=255)
                                                                                      cTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTaaa                                                                >  1:3910699/1‑150 (MQ=255)
                                                                                      cTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTaaa                                                                <  1:222474/150‑1 (MQ=255)
                                                                                            gCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGcc                                                          <  2:2394190/150‑1 (MQ=255)
                                                                                             cGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCt                                                         <  1:2260785/150‑1 (MQ=255)
                                                                                             cGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCt                                                         <  2:1493042/150‑1 (MQ=255)
                                                                                             cGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCt                                                         >  2:3510786/1‑150 (MQ=255)
                                                                                              gTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTg                                                        >  1:4244747/1‑150 (MQ=255)
                                                                                              gTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTg                                                        >  2:1564944/1‑150 (MQ=255)
                                                                                                 cGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATc                                                     >  1:4484353/1‑150 (MQ=255)
                                                                                                   tCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCtt                                                   <  1:31499/150‑1 (MQ=255)
                                                                                                    cccGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTc                                                  >  1:2312278/1‑150 (MQ=255)
                                                                                                     ccGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCa                                                 >  1:4538921/1‑150 (MQ=255)
                                                                                                     ccGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCa                                                 <  1:186786/150‑1 (MQ=255)
                                                                                                        gagaTAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAAcc                                              <  1:47408/150‑1 (MQ=255)
                                                                                                             aaTCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTgc                                         <  1:1340258/150‑1 (MQ=255)
                                                                                                              aTCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTgcg                                        >  1:3590277/1‑150 (MQ=255)
                                                                                                              aTCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTgcg                                        >  1:1077220/1‑150 (MQ=255)
                                                                                                                cAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGcgcg                                      >  2:1343093/1‑150 (MQ=255)
                                                                                                                cAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGcgcg                                      >  2:3621022/1‑150 (MQ=255)
                                                                                                                  aCGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGt                                    <  2:2596254/150‑1 (MQ=255)
                                                                                                                   cGGTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTa                                   >  1:3362167/1‑150 (MQ=255)
                                                                                                                   cGGTTTCTTCGCACCTACCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTa                                   <  2:2106496/150‑1 (MQ=255)
                                                                                                                    ggTTTCTTCGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTaa                                  <  2:2281484/150‑1 (MQ=255)
                                                                                                                    ggTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGATCGATACCGTCAACAGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACAGGTGCGCGGTaa                                  >  1:2415559/1‑150 (MQ=255)
                                                                                                                        tcttcGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTcc                              >  2:3052560/1‑150 (MQ=255)
                                                                                                                         cttcGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCa                             <  1:1061092/150‑1 (MQ=255)
                                                                                                                         cttcGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCa                             >  2:4545398/1‑150 (MQ=255)
                                                                                                                          ttcGCACCGGCCAGTGCCTGCAAGATTACGTCGCTCTTGCTTTGCAGCTGAGGTTCGATACCGTCTACCGCTTGTGCAGAGTTATCCAGCGCATGGGCGAGGGCTAAACCTAAACGCGCCTGTTCTTCAACCGGTGCTCGGTTAGTCCAc                            <  2:4370387/150‑1 (MQ=255)
                                                                                                                            cGCACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgc                          >  1:1572144/1‑150 (MQ=255)
                                                                                                                              cACCTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgccg                        >  1:398475/1‑150 (MQ=255)
                                                                                                                              cACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCAcgccg                        >  1:1102325/1‑150 (MQ=255)
                                                                                                                                ccTGCCAGTGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCcgcg                      >  1:462371/1‑150 (MQ=255)
                                                                                                                                ccTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCcgcg                      >  1:870482/1‑150 (MQ=255)
                                                                                                                                ccTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCcgcg                      <  2:2783671/150‑1 (MQ=255)
                                                                                                                                     cAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatc                 <  2:3360140/150‑1 (MQ=255)
                                                                                                                                     cAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatc                 >  1:3509449/1‑150 (MQ=255)
                                                                                                                                     cAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatc                 <  1:3962343/150‑1 (MQ=255)
                                                                                                                                      aGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatca                <  1:3098446/150‑1 (MQ=255)
                                                                                                                                        tGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatcatc              <  1:1264298/150‑1 (MQ=255)
                                                                                                                                        tGCCTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATatcatc              <  1:2326799/150‑1 (MQ=255)
                                                                                                                                          ccTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCa            >  2:2963511/1‑150 (MQ=255)
                                                                                                                                           cTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTTATCATCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAg           <  2:754727/150‑1 (MQ=255)
                                                                                                                                           cTGCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAg           <  1:4023210/150‑1 (MQ=255)
                                                                                                                                           cTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATACAg           >  1:3591068/1‑150 (MQ=255)
                                                                                                                                            tGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGa          >  1:3020381/1‑150 (MQ=255)
                                                                                                                                            tGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGa          >  2:2709289/1‑150 (MQ=255)
                                                                                                                                             gCACGATTACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGAc         <  2:4259736/150‑1 (MQ=255)
                                                                                                                                                cGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACggg      <  2:3994819/150‑1 (MQ=255)
                                                                                                                                                cGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACggg      <  2:449730/150‑1 (MQ=255)
                                                                                                                                                 gATTACGACGATTTTGCTTTGCCGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGgt     <  2:1178912/150‑1 (MQ=255)
                                                                                                                                                  aTGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGgtg    <  1:3217115/150‑1 (MQ=255)
                                                                                                                                                    gACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCATCCGGTGCGCGGTAAGTCCTCGCCGCGATATCATCCAGACGGGTGtc  <  2:1128716/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
AACGGGCAATCATGGTGATACCGACGTCAGCGCCGCGACCTTTCAGGGCTTTCGCGACGTTAGCCGCCGCCTGAATCACCTCTAAGCTACCGGCGTTCGTCCCGGAGATAATCAACGGTTTCTTCGCACCTGCCAGTGCCTGCACGATGACGTCGATTTTGCTTTGCAGCTCAGGTTCGATACCGTCAACCGCTGGTGCAGAGTTATCCAGCGCATGGGCGATGGCAAAACCTAAACGCGCCTGATCTTCAACCGGTGCGCGGTAAGTCCACGCCGCGATATCATCCAGACGGGTGTC  >  CP009273/2392042‑2392339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A