Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,098 T→G 51.8% S12A (TCT→GCT)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,0980TG51.8% 165.3 / inf 282S12A (TCT→GCT) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (67/68);  new base G (69/77);  total (137/145)
Fisher's exact test for biased strand distribution p-value = 7.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 287 total aligned reads displayed.

ATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCG  >  CP009273/3079950‑3080245
                                                                                                                                                    |                                                                                                                                                   
aTTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGtctc                                                                                                                                                    <  1:3188916/150‑1 (MQ=255)
 ttAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTcgct                                                                                                                                                   >  2:556759/1‑150 (MQ=255)
      acTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAgg                                                                                                                                              >  1:1227220/1‑150 (MQ=255)
      acTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAgg                                                                                                                                              <  2:3203706/150‑1 (MQ=255)
       cTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAggg                                                                                                                                             <  2:2148394/150‑1 (MQ=255)
        tCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGc                                                                                                                                            >  2:4123785/1‑150 (MQ=255)
          aGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCCCACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATTGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCAt                                                                                                                                          >  1:4331204/1‑150 (MQ=255)
          aGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTATTTATGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCAt                                                                                                                                          >  1:592890/1‑150 (MQ=255)
           gACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATc                                                                                                                                         >  2:3729278/1‑150 (MQ=255)
            aCTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATACTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATcc                                                                                                                                        <  2:1127196/150‑1 (MQ=255)
             cTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCt                                                                                                                                       <  1:3148574/150‑1 (MQ=255)
                ccAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGAc                                                                                                                                    <  1:398457/150‑1 (MQ=255)
                ccAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGAc                                                                                                                                    <  1:3503220/150‑1 (MQ=255)
                  aGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaa                                                                                                                                  <  2:2399440/150‑1 (MQ=255)
                   gtgtTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaa                                                                                                                                 >  2:858175/1‑150 (MQ=255)
                      tttttAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAAtt                                                                                                                              <  2:1240726/150‑1 (MQ=255)
                          tAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTg                                                                                                                          <  2:4040025/150‑1 (MQ=255)
                          tAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTg                                                                                                                          <  2:1735021/150‑1 (MQ=255)
                          tAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCATTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTg                                                                                                                          >  1:3329256/1‑150 (MQ=255)
                           aaCCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGa                                                                                                                         <  1:1551401/150‑1 (MQ=255)
                           aaCCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCCTCCTGACAAAATTGCTGa                                                                                                                         >  2:3891420/1‑150 (MQ=255)
                                gCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCaaa                                                                                                                    <  2:182668/150‑1 (MQ=255)
                                  agagTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAAtt                                                                                                                  <  2:4443292/150‑1 (MQ=255)
                                  agagTCGTGGTAGGATCCGCTACCACAGAAAATACACACAACAGTTTGAGCTAACCAAAATATGTTTAGGTGTTATTAAATATGGCAAAAAACCATTTTAAGTCCGAGTCCGTCGCTGAAGGGCAACCAGACAAAATTGATGACCaaaat                                                                                                                  >  1:139369/1‑148 (MQ=255)
                                   gagTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAAttt                                                                                                                 >  2:2801136/1‑150 (MQ=255)
                                     gTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCt                                                                                                               <  1:3281611/150‑1 (MQ=255)
                                       cGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGa                                                                                                             >  2:1701731/1‑150 (MQ=255)
                                        gTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGAt                                                                                                            >  1:2948456/1‑150 (MQ=255)
                                        gTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGAt                                                                                                            <  2:1198356/150‑1 (MQ=255)
                                               gATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGttt                                                                                                     <  2:3769973/150‑1 (MQ=255)
                                               gATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGttt                                                                                                     <  2:947481/150‑1 (MQ=255)
                                                     cTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGAcg                                                                                               >  2:3043206/1‑150 (MQ=255)
                                                     cTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGAcg                                                                                               <  2:3252192/150‑1 (MQ=255)
                                                         cacaGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGAt                                                                                           <  1:1454061/150‑1 (MQ=255)
                                                          acaGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATc                                                                                          >  1:82332/1‑150 (MQ=255)
                                                              aaaaTCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCg                                                                                      >  1:2651467/1‑150 (MQ=255)
                                                                aaTCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGaa                                                                                    >  2:3566263/1‑150 (MQ=255)
                                                                aaTCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGaa                                                                                    >  1:2057558/1‑150 (MQ=255)
                                                                       acaacaGTTTGAGCTAACCAACTTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATc                                                                             <  2:4376239/150‑1 (MQ=255)
                                                                          acaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGa                                                                          >  1:1646198/1‑150 (MQ=255)
                                                                          acaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGa                                                                          <  1:291562/150‑1 (MQ=255)
                                                                                tGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc                                                                    <  2:2509170/150‑1 (MQ=255)
                                                                                tGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAc                                                                    <  1:541686/150‑1 (MQ=255)
                                                                                 gAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAcg                                                                   >  2:1569993/1‑150 (MQ=255)
                                                                                   gCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAcgcg                                                                 <  1:4216136/150‑1 (MQ=255)
                                                                                   gCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCAcgcg                                                                 <  1:3663606/150‑1 (MQ=255)
                                                                                    cTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGt                                                                >  1:4069153/1‑150 (MQ=255)
                                                                                     tAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGtt                                                               >  1:886931/1‑150 (MQ=255)
                                                                                      aaCCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttg                                                              <  2:2355676/150‑1 (MQ=255)
                                                                                      aaCCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttg                                                              <  1:914213/150‑1 (MQ=255)
                                                                                       aCCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgc                                                             >  1:3796321/1‑150 (MQ=255)
                                                                                        ccAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgct                                                            <  2:1983087/150‑1 (MQ=255)
                                                                                        ccAAATTCTCTTGAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgct                                                            <  2:432205/150‑1 (MQ=255)
                                                                                         cAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgctt                                                           <  2:481054/150‑1 (MQ=255)
                                                                                         cAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgctt                                                           >  2:3420380/1‑150 (MQ=255)
                                                                                           aaTTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgcttgc                                                         <  1:2182499/150‑1 (MQ=255)
                                                                                            aTTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCg                                                        >  2:4130326/1‑150 (MQ=255)
                                                                                              tctcTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa                                                      >  2:4279630/1‑150 (MQ=255)
                                                                                              tctcTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa                                                      >  2:2394471/1‑150 (MQ=255)
                                                                                                tctTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc                                                    <  2:3846198/150‑1 (MQ=255)
                                                                                                 ctTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAcc                                                   >  1:1518426/1‑150 (MQ=255)
                                                                                                  tttAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCt                                                  <  2:1473093/150‑1 (MQ=255)
                                                                                                       gTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTa                                             <  1:4542635/150‑1 (MQ=255)
                                                                                                       gTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTa                                             >  1:2524608/1‑150 (MQ=255)
                                                                                                       gTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTa                                             >  1:671615/1‑150 (MQ=255)
                                                                                                          atatTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGACAGCACGCGTTGCTTGCGAAACCTACGTaaaa                                          <  1:1823319/150‑1 (MQ=255)
                                                                                                          atatTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTaaaa                                          <  1:1609648/150‑1 (MQ=255)
                                                                                                             ttAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAAcc                                       >  2:2721086/1‑150 (MQ=255)
                                                                                                              tAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCg                                      >  2:4392459/1‑150 (MQ=255)
                                                                                                               aaaTATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCgg                                     <  1:341929/150‑1 (MQ=255)
                                                                                                               aaaTATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCgg                                     >  2:4199807/1‑150 (MQ=255)
                                                                                                                  tatGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAt                                  <  2:4161482/150‑1 (MQ=255)
                                                                                                                  tatGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAt                                  >  1:4008347/1‑150 (MQ=255)
                                                                                                                   atGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATg                                 <  2:4489318/150‑1 (MQ=255)
                                                                                                                   atGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATg                                 >  1:97470/1‑150 (MQ=255)
                                                                                                                   atGGCAAAACACCTTTGTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATg                                 <  1:3096783/150‑1 (MQ=255)
                                                                                                                    tGGCACAACACCTTTTTACGTCCGAGTCCGTCGCTGACGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATgg                                >  2:808621/1‑150 (MQ=255)
                                                                                                                       cAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGttt                             >  1:2748039/1‑150 (MQ=255)
                                                                                                                         aaaCACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTa                           <  1:3177715/150‑1 (MQ=255)
                                                                                                                           acacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGt                         <  1:409400/150‑1 (MQ=255)
                                                                                                                           acacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAGGGTTTTAGt                         >  2:3590116/1‑150 (MQ=255)
                                                                                                                            cacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                        >  1:2493592/1‑150 (MQ=255)
                                                                                                                            cacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                        >  1:4042316/1‑150 (MQ=255)
                                                                                                                            cacCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                        <  2:1426833/150‑1 (MQ=255)
                                                                                                                            cacCTTTTTACGTACGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                        <  2:1159286/150‑1 (MQ=255)
                                                                                                                              ccTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTgg                      >  1:1859578/1‑150 (MQ=255)
                                                                                                                                   ttACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGgcggcg                 >  2:4020279/1‑150 (MQ=255)
                                                                                                                                      cGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTGTTAGTTGGCGGCGaaa              >  1:2388954/1‑150 (MQ=255)
                                                                                                                                       gTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAAt             >  1:465881/1‑150 (MQ=255)
                                                                                                                                        tCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATc            >  1:1130386/1‑150 (MQ=255)
                                                                                                                                         ccGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATca           <  1:124102/150‑1 (MQ=255)
                                                                                                                                           gAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAAAAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcacc         <  1:3690534/150‑1 (MQ=255)
                                                                                                                                            aGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcacca        <  2:708491/150‑1 (MQ=255)
                                                                                                                                            aGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcacca        <  1:1099745/150‑1 (MQ=255)
                                                                                                                                            aGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcacca        <  1:3741219/150‑1 (MQ=255)
                                                                                                                                             gTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcaccac       <  2:2561702/150‑1 (MQ=255)
                                                                                                                                               ccGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCAccacca     >  2:1619143/1‑150 (MQ=255)
                                                                                                                                                cGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAg    >  2:2268801/1‑150 (MQ=255)
                                                                                                                                                 gTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAgc   <  1:3605509/150‑1 (MQ=255)
                                                                                                                                                 gTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAgc   <  2:2985013/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
ATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCG  >  CP009273/3079950‑3080245

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A