Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,080,098 T→G 38.3% S12A (TCT→GCT)  metK → S‑adenosylmethionine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,080,0980TG38.3% 107.7 / 309.4 225S12A (TCT→GCT) metKS‑adenosylmethionine synthetase
Reads supporting (aligned to +/- strand):  ref base T (77/62);  new base G (45/41);  total (122/103)
Fisher's exact test for biased strand distribution p-value = 6.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 227 total aligned reads displayed.

GATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCC  >  CP009273/3079949‑3080247
                                                                                                                                                     |                                                                                                                                                     
    aaCACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGa                                                                                                                                                   >  2:267112/1‑150 (MQ=255)
    aaCACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGa                                                                                                                                                   <  1:2554940/150‑1 (MQ=255)
        cTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAggg                                                                                                                                               >  2:1370208/1‑150 (MQ=255)
          cAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCCCACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCa                                                                                                                                             <  1:2635901/150‑1 (MQ=255)
            gACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCCCAGAAAATCCACACAACCGTTTGAGCTAACCAAATTCTCGGTAGGTGATATTAAATATTGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATc                                                                                                                                           >  2:41317/1‑150 (MQ=255)
              cTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCt                                                                                                                                         >  2:310675/1‑150 (MQ=255)
               tGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTACGAGTCCGTCTCTGAAGGGCATccgg                                                                                                                                        >  1:3513130/1‑148 (MQ=255)
               tGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAAAACCATTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTg                                                                                                                                        >  1:4047881/1‑150 (MQ=255)
                gCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAAAACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGa                                                                                                                                       >  1:3754192/1‑150 (MQ=255)
                 ccAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGAc                                                                                                                                      <  1:2655833/150‑1 (MQ=255)
                  cAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACa                                                                                                                                     <  2:4053058/150‑1 (MQ=255)
                  cAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCATTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACa                                                                                                                                     >  1:1189513/1‑150 (MQ=255)
                     tgtTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaaa                                                                                                                                  <  1:1908475/150‑1 (MQ=255)
                     tgtTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACACTTTGAGCTAACCAAATTATATTTAGGTGATATTAAATATTGCAAAACACCTTTTTACCTCCGAGTCCGTCTCTGAAGGGCATCCTGACaaaa                                                                                                                                  >  1:192967/1‑150 (MQ=255)
                       tttttATCCTGCAGAGTCGTGGTTGGATCCGCGACCACAGAAAATCCCCACAACAGTTTGAGCTAAACAATTTCTCTTTAGGTGATATTAAATATGTCAAAACACCTTTTTAAGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAAtt                                                                                                                                <  2:1558519/150‑1 (MQ=255)
                       tttttAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAAtt                                                                                                                                <  2:3844342/150‑1 (MQ=255)
                         tttAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCGTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGCAGGGCATGCTGACAAAATTGc                                                                                                                              >  2:1497162/1‑150 (MQ=255)
                           tAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTg                                                                                                                            >  1:3605237/1‑150 (MQ=255)
                            aaCCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTACGTCTCTGAAGGGCATCCTGACAAAATTGCTGa                                                                                                                           >  1:1589747/1‑150 (MQ=255)
                            aaCCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATACACCCAACAGTTTGAGCTAAACAAATTCTCTTTAGGTGATATTAAATATTGCAAAACACCATTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCAGACAAAATTTCtta                                                                                                                           >  1:3845644/1‑148 (MQ=255)
                             aCCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGAc                                                                                                                          <  1:391160/150‑1 (MQ=255)
                              ccTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGAcc                                                                                                                         >  1:1029268/1‑150 (MQ=255)
                                      gTCGTGGTAGGATCCGCTACCACAGACTTTCCACCCAACAGTTTGAGCTAACGTAATTCTCTTGAGGTGATTTTTAATATGGCAATACAGCTTTTTTCGTCCGTGTGCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCt                                                                                                                 <  1:1673440/150‑1 (MQ=255)
                                        cGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGa                                                                                                               >  1:1003663/1‑150 (MQ=255)
                                             tAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCg                                                                                                          <  1:558037/150‑1 (MQ=255)
                                               ggATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGtt                                                                                                        >  1:2050022/1‑150 (MQ=255)
                                               ggATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGtt                                                                                                        >  1:4124171/1‑150 (MQ=255)
                                               ggATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGtt                                                                                                        >  2:2248777/1‑150 (MQ=255)
                                                gATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGttt                                                                                                       <  1:893855/150‑1 (MQ=255)
                                                 aTCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGtttt                                                                                                      >  1:1356201/1‑150 (MQ=255)
                                                 aTCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGCGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGtttt                                                                                                      >  1:3450263/1‑150 (MQ=255)
                                                   ccGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAg                                                                                                    <  2:2383015/150‑1 (MQ=255)
                                                        aCCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGAcgcg                                                                                               >  1:2176273/1‑150 (MQ=255)
                                                        aCCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGAcgcg                                                                                               <  2:1824590/150‑1 (MQ=255)
                                                         ccACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGa                                                                                              >  1:1629198/1‑150 (MQ=255)
                                                          cacaGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGAt                                                                                             <  2:524793/150‑1 (MQ=255)
                                                             aGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCt                                                                                          <  2:21912/150‑1 (MQ=255)
                                                             aGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCt                                                                                          <  1:1694775/150‑1 (MQ=255)
                                                              gAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTc                                                                                         >  1:148434/1‑150 (MQ=255)
                                                                aaaTCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGa                                                                                       >  2:2510588/1‑150 (MQ=255)
                                                                   tCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACa                                                                                    <  2:3693623/150‑1 (MQ=255)
                                                                    ccACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAg                                                                                   <  2:746906/150‑1 (MQ=255)
                                                                       cacaACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGAt                                                                                <  2:806179/150‑1 (MQ=255)
                                                                       cacaACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGAt                                                                                <  1:3396968/150‑1 (MQ=255)
                                                                        acaacaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATc                                                                               <  1:2096395/150‑1 (MQ=255)
                                                                        acaacaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATc                                                                               <  2:1262942/150‑1 (MQ=255)
                                                                        acaacaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATc                                                                               >  2:2282800/1‑150 (MQ=255)
                                                                         caacaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATcc                                                                              >  1:2427342/1‑150 (MQ=255)
                                                                          atcaGTTTGATCTAACCAAATTCTCTTTAGGTTATATTAAATATGTCAATACACCTTTTTACGTCCGAGTGCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGACGTTTTAGACGCGATCCTCGAACAGGATCCg                                                                             <  1:3872650/148‑1 (MQ=255)
                                                                          aacaGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCg                                                                             >  2:2511204/1‑150 (MQ=255)
                                                                             aGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGaaa                                                                          <  1:2848080/150‑1 (MQ=255)
                                                                              gTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg                                                                         <  2:1594429/150‑1 (MQ=255)
                                                                              gTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg                                                                         >  2:3493058/1‑150 (MQ=255)
                                                                              gTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAg                                                                         >  2:2568149/1‑150 (MQ=255)
                                                                               tttGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGc                                                                        >  2:3794636/1‑150 (MQ=255)
                                                                                      tAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGtt                                                                 <  1:4169488/150‑1 (MQ=255)
                                                                                      tAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGtt                                                                 <  2:3993687/150‑1 (MQ=255)
                                                                                       aaCCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttg                                                                >  1:1117139/1‑150 (MQ=255)
                                                                                         ccAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgct                                                              >  1:3746821/1‑150 (MQ=255)
                                                                                         caaaaTTCTCTTTAGGTTATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgct                                                              <  1:3285306/148‑1 (MQ=255)
                                                                                            aaTTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGttgcttgc                                                           >  2:4009525/1‑150 (MQ=255)
                                                                                             aTTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCg                                                          <  1:178023/150‑1 (MQ=255)
                                                                                              ttCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGa                                                         >  2:2863551/1‑150 (MQ=255)
                                                                                               tctcTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa                                                        <  1:310675/150‑1 (MQ=255)
                                                                                               tctcTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaa                                                        >  2:2280720/1‑150 (MQ=255)
                                                                                                ctctTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGaaa                                                       >  2:3877330/1‑150 (MQ=255)
                                                                                                 tctTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAc                                                      >  1:2288045/1‑150 (MQ=255)
                                                                                                  ctTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAAcc                                                     >  1:2808871/1‑150 (MQ=255)
                                                                                                         tGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTaa                                              <  1:3581035/150‑1 (MQ=255)
                                                                                                         tGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTaa                                              >  2:761518/1‑150 (MQ=255)
                                                                                                         tGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTaa                                              >  1:2526724/1‑150 (MQ=255)
                                                                                                           atatTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTaaaa                                            >  1:3462721/1‑150 (MQ=255)
                                                                                                             atTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAAc                                          >  1:1682894/1‑150 (MQ=255)
                                                                                                              ttAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAAcc                                         <  2:3524112/150‑1 (MQ=255)
                                                                                                               tAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCg                                        <  1:536800/150‑1 (MQ=255)
                                                                                                               tAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCg                                        >  2:1672621/1‑150 (MQ=255)
                                                                                                               tAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATACTGACAAAATTGATGACCAAATTTATGATGCCTTTTTAGACGCTATCATCGAACAGGATAAGAAAGCACGCGTTGCTTGCGAAACCTACGTAAATAcct                                        >  1:3001792/1‑149 (MQ=255)
                                                                                                               tAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCg                                        >  2:4097539/1‑150 (MQ=255)
                                                                                                                 aaTATGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGc                                      >  1:629729/1‑150 (MQ=255)
                                                                                                                   tatGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAt                                    <  2:3176404/150‑1 (MQ=255)
                                                                                                                   tatGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAt                                    <  2:1873627/150‑1 (MQ=255)
                                                                                                                   tatGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCAt                                    <  2:2227542/150‑1 (MQ=255)
                                                                                                                    atGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATg                                   >  2:3989478/1‑150 (MQ=255)
                                                                                                                    atGGCAAAACACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATg                                   >  1:494442/1‑150 (MQ=255)
                                                                                                                      ggCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGt                                 >  1:1888355/1‑150 (MQ=255)
                                                                                                                         aaaaCACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGtttt                              >  2:652281/1‑150 (MQ=255)
                                                                                                                          aaaCACCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTa                             <  1:702250/150‑1 (MQ=255)
                                                                                                                            acacCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGt                           >  1:316091/1‑150 (MQ=255)
                                                                                                                            acacCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGt                           >  1:2994772/1‑150 (MQ=255)
                                                                                                                             cacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                          <  1:1956997/150‑1 (MQ=255)
                                                                                                                             cacCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                          <  1:233465/150‑1 (MQ=255)
                                                                                                                             cacCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGtt                          <  1:1417531/150‑1 (MQ=255)
                                                                                                                              acCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTg                         >  1:2467407/1‑150 (MQ=255)
                                                                                                                              acCTTTTTACGTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTg                         >  2:2166393/1‑150 (MQ=255)
                                                                                                                                cTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTggc                       <  2:2041708/150‑1 (MQ=255)
                                                                                                                                    ttACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGgcggcg                   >  2:2150550/1‑150 (MQ=255)
                                                                                                                                        gTCCGAGTCCGTCGCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAAt               >  2:1001568/1‑150 (MQ=255)
                                                                                                                                            gAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATcacc           >  2:1215570/1‑150 (MQ=255)
                                                                                                                                               tCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCaccacc        <  1:3975272/150‑1 (MQ=255)
                                                                                                                                                ccGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCAccacca       <  2:3548609/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATTAACACTCAGACTGCCAGTGTTTTTAACCTGCAGAGTCGTGGTAGGATCCGCTACCACAGAAAATCCACACAACAGTTTGAGCTAACCAAATTCTCTTTAGGTGATATTAAATATGGCAAAACACCTTTTTACGTCCGAGTCCGTCTCTGAAGGGCATCCTGACAAAATTGCTGACCAAATTTCTGATGCCGTTTTAGACGCGATCCTCGAACAGGATCCGAAAGCACGCGTTGCTTGCGAAACCTACGTAAAAACCGGCATGGTTTTAGTTGGCGGCGAAATCACCACCAGCGCC  >  CP009273/3079949‑3080247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A