Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,104,247 C→A 15.3% R80L (CGA→CTA)  yghD ← putative membrane‑anchored secretion pathway M‑type protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,104,2470CA15.3% 889.3 / 95.6 274R80L (CGA→CTA) yghDputative membrane‑anchored secretion pathway M‑type protein
Reads supporting (aligned to +/- strand):  ref base C (113/119);  new base A (20/22);  total (133/141)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01

Only 100 of 275 total aligned reads displayed.

GACCTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACGCCCC  >  CP009273/3104099‑3104396
                                                                                                                                                    |                                                                                                                                                     
gACCTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCg                                                                                                                                                      >  2:1968120/1‑150 (MQ=255)
  ccTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCggg                                                                                                                                                    <  1:3602835/150‑1 (MQ=255)
  ccTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCggg                                                                                                                                                    >  2:3965248/1‑150 (MQ=255)
   cTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGgt                                                                                                                                                   >  1:1002603/1‑150 (MQ=255)
    tGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGgtg                                                                                                                                                  >  1:1852133/1‑150 (MQ=255)
        tatTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCa                                                                                                                                              >  1:3220246/1‑150 (MQ=255)
          ttCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATc                                                                                                                                            <  1:2282175/150‑1 (MQ=255)
          ttCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATc                                                                                                                                            <  2:1269253/150‑1 (MQ=255)
           tctcCCCACGATCGGCTAGCCGCCTGATGACCACCGAGTGCGCGGAAGCTCTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCg                                                                                                                                           <  2:3677697/150‑1 (MQ=255)
                ccACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGccacc                                                                                                                                      >  2:2376146/1‑150 (MQ=255)
                 cACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGccacca                                                                                                                                     >  2:4104189/1‑150 (MQ=255)
                 cACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGccacca                                                                                                                                     >  1:3797462/1‑150 (MQ=255)
                 cACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGccacca                                                                                                                                     <  1:4621044/150‑1 (MQ=255)
                  aCGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAg                                                                                                                                    <  1:2979731/150‑1 (MQ=255)
                    gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCCGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgct                                                                                                                                  <  2:4624012/150‑1 (MQ=255)
                    gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgct                                                                                                                                  >  2:3317363/1‑150 (MQ=255)
                    gATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgct                                                                                                                                  <  2:167932/150‑1 (MQ=255)
                     aTCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctg                                                                                                                                 >  1:179414/1‑150 (MQ=255)
                     aTCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCTCCGgctg                                                                                                                                 <  2:3471157/150‑1 (MQ=255)
                      tCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgc                                                                                                                                >  2:4155648/1‑150 (MQ=255)
                      tCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgc                                                                                                                                >  1:4171736/1‑150 (MQ=255)
                       cGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGTTGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgct                                                                                                                               <  1:1010718/150‑1 (MQ=255)
                       cGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAgctgct                                                                                                                               <  2:3235556/150‑1 (MQ=255)
                         gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCtgctgc                                                                                                                             >  2:2779089/1‑150 (MQ=255)
                         gCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCtgctgc                                                                                                                             >  2:674230/1‑150 (MQ=255)
                          cTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCa                                                                                                                            <  1:1653292/150‑1 (MQ=255)
                             tCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAata                                                                                                                         >  1:2193628/1‑150 (MQ=255)
                              ccGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAatat                                                                                                                        <  1:4149237/150‑1 (MQ=255)
                               cGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATAtt                                                                                                                       >  1:3335298/1‑150 (MQ=255)
                               cGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATAtt                                                                                                                       <  1:249767/150‑1 (MQ=255)
                                  cTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGtc                                                                                                                    <  1:3196458/150‑1 (MQ=255)
                                    gatgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                                  >  2:1696712/1‑150 (MQ=255)
                                    gatgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGtctc                                                                                                                  >  2:2352776/1‑150 (MQ=255)
                                      tgaCCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCt                                                                                                                <  2:2474183/150‑1 (MQ=255)
                                        accaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGt                                                                                                              <  1:1750612/150‑1 (MQ=255)
                                         ccaccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGtt                                                                                                             >  1:2780844/1‑150 (MQ=255)
                                          caccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTg                                                                                                            <  1:737660/150‑1 (MQ=255)
                                            ccGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCt                                                                                                          <  2:2119870/150‑1 (MQ=255)
                                             cGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTc                                                                                                         <  1:3209523/150‑1 (MQ=255)
                                                gTGCGCGGAAGCTCTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                                      <  1:95032/150‑1 (MQ=255)
                                                gTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                                      <  2:878866/150‑1 (MQ=255)
                                                gTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                                      <  1:664214/150‑1 (MQ=255)
                                                gTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAAt                                                                                                      <  1:1985059/150‑1 (MQ=255)
                                                  gcgcGGAAGCACTGTCACTGAAGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATcc                                                                                                    <  1:780997/150‑1 (MQ=255)
                                                   cgcgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCg                                                                                                   <  1:3226685/150‑1 (MQ=255)
                                                     cgGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGtt                                                                                                 >  2:193709/1‑150 (MQ=255)
                                                          gCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTc                                                                                            >  1:2740969/1‑150 (MQ=255)
                                                               gTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACgg                                                                                       >  2:786403/1‑150 (MQ=255)
                                                                 cACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCt                                                                                     <  1:2840042/150‑1 (MQ=255)
                                                                  aCTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTg                                                                                    >  1:2321426/1‑150 (MQ=255)
                                                                  aCTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTg                                                                                    >  2:163676/1‑150 (MQ=255)
                                                                   cTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGc                                                                                   <  1:1079829/150‑1 (MQ=255)
                                                                    tgatgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGcc                                                                                  <  2:1512268/150‑1 (MQ=255)
                                                                     gatgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCa                                                                                 <  1:867989/150‑1 (MQ=255)
                                                                     gatgaCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCa                                                                                 <  1:197959/150‑1 (MQ=255)
                                                                         aCCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATg                                                                             <  1:4371920/150‑1 (MQ=255)
                                                                            cgcgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAgg                                                                          <  1:3475359/150‑1 (MQ=255)
                                                                             gcgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCGGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGa                                                                         <  2:2328927/150‑1 (MQ=255)
                                                                              cgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGAc                                                                        >  2:4326947/1‑150 (MQ=255)
                                                                              cgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGAc                                                                        >  2:4341371/1‑150 (MQ=255)
                                                                              cgATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGAc                                                                        >  2:4341062/1‑150 (MQ=255)
                                                                                  aCCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGAGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGATCGCTCACCGGCTGCCAGATGAGGACGTaa                                                                    >  2:1345424/1‑150 (MQ=255)
                                                                                   ccTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAat                                                                   <  1:3084955/150‑1 (MQ=255)
                                                                                       cgcAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATcc                                                               >  1:152389/1‑150 (MQ=255)
                                                                                          aGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGAc                                                            >  1:1787919/1‑150 (MQ=255)
                                                                                          aGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGAc                                                            <  2:3082471/150‑1 (MQ=255)
                                                                                          aGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGAc                                                            <  2:2672416/150‑1 (MQ=255)
                                                                                          aGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGAc                                                            <  2:4222214/150‑1 (MQ=255)
                                                                                           ggCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACg                                                           <  1:337602/150‑1 (MQ=255)
                                                                                             cGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCt                                                         >  2:1237955/1‑150 (MQ=255)
                                                                                               ttgttgTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTaa                                                       >  1:2468690/1‑150 (MQ=255)
                                                                                                    gtgGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAgg                                                  <  1:2946846/150‑1 (MQ=255)
                                                                                                    gtgGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAgg                                                  <  1:1244494/150‑1 (MQ=255)
                                                                                                     tgGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGa                                                 <  1:3090335/150‑1 (MQ=255)
                                                                                                     tgGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGa                                                 >  1:3498716/1‑150 (MQ=255)
                                                                                                            ttttCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAAcaccac                                          <  2:1443646/150‑1 (MQ=255)
                                                                                                               tCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGc                                       >  2:57277/1‑150 (MQ=255)
                                                                                                                  cgcTGCAATAATATCCGGCGCGGCGTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGc                                    >  1:4097184/1‑150 (MQ=255)
                                                                                                                       cAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTa                               >  1:1310457/1‑150 (MQ=255)
                                                                                                                              atCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGcc                        <  1:3715505/150‑1 (MQ=255)
                                                                                                                              atCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGcc                        >  1:580245/1‑150 (MQ=255)
                                                                                                                               tCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGccc                       <  2:2525436/150‑1 (MQ=255)
                                                                                                                                  ggcgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCttt                    >  2:2218598/1‑150 (MQ=255)
                                                                                                                                   gcgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCtttt                   <  2:3401856/150‑1 (MQ=255)
                                                                                                                                    cgcggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTc                  <  2:3610334/150‑1 (MQ=255)
                                                                                                                                      cggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAc                >  2:1522161/1‑150 (MQ=255)
                                                                                                                                       ggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcg               >  1:3670305/1‑150 (MQ=255)
                                                                                                                                       ggcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcg               >  2:263961/1‑150 (MQ=255)
                                                                                                                                       ggcgTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcg               >  1:973682/1‑150 (MQ=255)
                                                                                                                                        gcgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgc              <  2:3797492/150‑1 (MQ=255)
                                                                                                                                         cgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgcg             >  2:1067170/1‑150 (MQ=255)
                                                                                                                                         cgTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCAcgcg             <  1:4424664/150‑1 (MQ=255)
                                                                                                                                          gTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCgg            >  2:189982/1‑150 (MQ=255)
                                                                                                                                          gTTCTTCAGTAGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCgg            >  2:1109751/1‑150 (MQ=255)
                                                                                                                                           ttcttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGga           <  2:2901943/150‑1 (MQ=255)
                                                                                                                                           ttcttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGga           >  1:3914279/1‑150 (MQ=255)
                                                                                                                                            tcttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGgag          <  1:1447683/150‑1 (MQ=255)
                                                                                                                                            tcttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGgag          >  2:4452636/1‑150 (MQ=255)
                                                                                                                                              ttcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGaa        <  2:2797003/150‑1 (MQ=255)
                                                                                                                                               tcAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAAc       <  2:2345399/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GACCTGAATATTCTCCCCACGATCGGCTATCCGCCTGATGACCACCGAGTGCGCGGAAGCACTGTCACTGATGACCCGCGATACCTGCGCAGGCGTTGTTGTGGCAGATTTTCGCGCTGCAATAATATCCGGCGCGGCGTTCTTCAGTCGGGTGTTCATCGCCACCAGCTGCTGCAATATTGTCTCCTGTTGCTCAATCCGTTCGCTCAACGGCTGCCAGATGAGGACGTAATATCCGACGCTAAACAGGAACACCACCGCTGCCAGTAACATGCCCTTTTCACGCGGAGAACGCCCC  >  CP009273/3104099‑3104396

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A