Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,229,926 C→A 49.4% L10I (CTC→ATC)  ygjQ → DUF218 superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,229,9260CA49.4% 146.5 / inf 286L10I (CTC→ATC) ygjQDUF218 superfamily protein
Reads supporting (aligned to +/- strand):  ref base C (77/67);  new base A (89/53);  total (166/120)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 305 total aligned reads displayed.

CCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAAC  >  CP009273/3229777‑3230075
                                                                                                                                                     |                                                                                                                                                     
 cGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTtcttct                                                                                                                                                      <  2:2101798/150‑1 (MQ=255)
   aCTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATcc                                                                                                                                                    >  2:3791109/1‑150 (MQ=255)
        ggCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTAtt                                                                                                                                               <  2:1659897/150‑1 (MQ=255)
         gCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTAttt                                                                                                                                              <  2:3393576/150‑1 (MQ=255)
          cTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTg                                                                                                                                             <  1:955938/150‑1 (MQ=255)
             aCGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAACTCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGttt                                                                                                                                          <  2:2241209/150‑1 (MQ=255)
              cGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGtttt                                                                                                                                         >  1:1446942/1‑150 (MQ=255)
                  gTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTtctc                                                                                                                                     >  1:1557437/1‑150 (MQ=255)
                     gTCTCTTGGGCAGATCAAAATCTGAAATTGATGTCATGCTTTATCTTTTCTTTATTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGAATTTTCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGca                                                                                                                                  <  2:2586408/150‑2 (MQ=255)
                      tCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCg                                                                                                                                 >  1:2973392/1‑150 (MQ=255)
                       cGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCgg                                                                                                                                >  2:507135/1‑150 (MQ=255)
                          ttGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGAct                                                                                                                             <  1:3190600/150‑1 (MQ=255)
                             ggCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTa                                                                                                                          <  2:1384335/150‑1 (MQ=255)
                               cAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTaaa                                                                                                                        >  1:2078183/1‑150 (MQ=255)
                                 gAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGa                                                                                                                      >  1:23495/1‑150 (MQ=255)
                                  aaCAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGAt                                                                                                                     >  1:4522712/1‑150 (MQ=255)
                                  aaCAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGAt                                                                                                                     <  2:1489694/150‑1 (MQ=255)
                                   aCAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTAGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGAtt                                                                                                                    <  1:2664025/150‑1 (MQ=255)
                                    cAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTg                                                                                                                   <  1:1647788/150‑1 (MQ=255)
                                     aaaaTCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGc                                                                                                                  <  2:42376/150‑1 (MQ=255)
                                       aaTCTGAAAATGATGTCAGGCTTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTTCCCGCATTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCt                                                                                                                <  2:1260591/150‑1 (MQ=255)
                                         tCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGc                                                                                                              <  1:3985816/150‑1 (MQ=255)
                                           tGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCtt                                                                                                            <  1:1087417/150‑1 (MQ=255)
                                            gAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTa                                                                                                           >  2:1075201/1‑150 (MQ=255)
                                             aaaaTGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCATTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTAc                                                                                                          >  1:1497556/1‑150 (MQ=255)
                                              aaaTGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACt                                                                                                         >  1:2899824/1‑150 (MQ=255)
                                               aaTGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTg                                                                                                        >  2:3325499/1‑150 (MQ=255)
                                               aaTGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTg                                                                                                        <  2:4603048/150‑1 (MQ=255)
                                                 tgatgTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGtt                                                                                                      >  1:1735532/1‑150 (MQ=255)
                                                     gTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACtt                                                                                                  >  2:4095258/1‑150 (MQ=255)
                                                      tCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTtg                                                                                                 >  2:3671145/1‑150 (MQ=255)
                                                        aGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTtgtg                                                                                               <  1:3078690/150‑1 (MQ=255)
                                                        aGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTtgtg                                                                                               <  2:4246738/150‑1 (MQ=255)
                                                         ggCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTgg                                                                                              >  1:3838381/1‑150 (MQ=255)
                                                          gCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGc                                                                                             <  2:1567873/150‑1 (MQ=255)
                                                            gTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAg                                                                                           >  2:3805134/1‑150 (MQ=255)
                                                              tATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGa                                                                                         >  1:504064/1‑150 (MQ=255)
                                                              tATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGa                                                                                         >  2:1394592/1‑150 (MQ=255)
                                                                tcttttctttCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGc                                                                                       <  1:2937946/150‑1 (MQ=255)
                                                                  ttttctttCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGa                                                                                     >  2:1862709/1‑150 (MQ=255)
                                                                    ttctttctTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGAcc                                                                                   <  2:414410/150‑1 (MQ=255)
                                                                      ctttctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCAt                                                                                 <  2:4611994/150‑1 (MQ=255)
                                                                       tttctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATc                                                                                <  1:1146516/150‑1 (MQ=255)
                                                                          ctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCtt                                                                             <  1:2438967/150‑1 (MQ=255)
                                                                          ctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCtt                                                                             <  2:4203943/150‑1 (MQ=255)
                                                                          ctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCtt                                                                             >  2:4473931/1‑150 (MQ=255)
                                                                          ctttAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCtt                                                                             >  1:1314808/1‑150 (MQ=255)
                                                                             tAGCGCCTAACATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTTCTTGTGGCAGGAGCGACCTTCCTTATc                                                                          <  1:4225675/150‑1 (MQ=255)
                                                                                cgcCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGcc                                                                       <  1:4103844/150‑1 (MQ=255)
                                                                                  ccTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGa                                                                     <  1:775570/150‑1 (MQ=255)
                                                                                  ccTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGa                                                                     >  1:3725250/1‑150 (MQ=255)
                                                                                   cTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGAt                                                                    >  1:668714/1‑150 (MQ=255)
                                                                                     aaaaTCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATcg                                                                  >  1:36105/1‑150 (MQ=255)
                                                                                       aaTCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATcgcg                                                                >  1:3424101/1‑150 (MQ=255)
                                                                                        aTCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGt                                                               >  2:1553076/1‑150 (MQ=255)
                                                                                         tCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTa                                                              >  2:2321974/1‑150 (MQ=255)
                                                                                           gACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAAt                                                            >  2:3120715/1‑150 (MQ=255)
                                                                                              cTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGt                                                         >  2:1989838/1‑150 (MQ=255)
                                                                                              cTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGt                                                         >  1:993852/1‑150 (MQ=255)
                                                                                              cTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGt                                                         >  1:993652/1‑150 (MQ=255)
                                                                                                ccccccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGAGTAATGGTCa                                                       >  2:2180021/1‑150 (MQ=255)
                                                                                                 cccccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCaa                                                      >  2:544047/1‑150 (MQ=255)
                                                                                                 cccccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCaa                                                      >  2:2964387/1‑150 (MQ=255)
                                                                                                 cccccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCaa                                                      >  2:2815704/1‑150 (MQ=255)
                                                                                                    ccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGc                                                   >  1:1168730/1‑150 (MQ=255)
                                                                                                    ccTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGc                                                   >  1:621467/1‑150 (MQ=255)
                                                                                                     cTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTCATGGTCAACGcc                                                  >  1:2147904/1‑150 (MQ=255)
                                                                                                      tttCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCa                                                 <  1:3279415/150‑1 (MQ=255)
                                                                                                      tttCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCa                                                 >  1:4450558/1‑150 (MQ=255)
                                                                                                          gTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCaa                                             >  2:3908513/1‑150 (MQ=255)
                                                                                                          gTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCaa                                             <  2:3049761/150‑1 (MQ=255)
                                                                                                           ttAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCaaa                                            <  1:1454179/150‑1 (MQ=255)
                                                                                                            tAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAAc                                           >  2:2490562/1‑150 (MQ=255)
                                                                                                              aGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAg                                         <  2:1047222/150‑1 (MQ=255)
                                                                                                                 aTAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCtn                                      <  2:1517640/150‑2 (MQ=255)
                                                                                                                   aaCGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCCACGCCAGCAAACAGCTGAc                                    >  2:3490819/1‑150 (MQ=255)
                                                                                                                      gACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTg                                 >  2:581407/1‑150 (MQ=255)
                                                                                                                      gACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTg                                 >  2:4038728/1‑150 (MQ=255)
                                                                                                                      gACCATGCTGCGCGCATATGCCCGCCTTCTTCTCCGTATTTGTTTTTATCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTg                                 >  2:1714226/1‑150 (MQ=255)
                                                                                                                       aCCATGCTGCGCTCATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTgg                                >  1:1884371/1‑150 (MQ=255)
                                                                                                                       aCCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTgg                                >  1:1415996/1‑150 (MQ=255)
                                                                                                                        ccATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGa                               <  1:3781339/150‑1 (MQ=255)
                                                                                                                            gctgcGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGa                           <  1:2289109/150‑1 (MQ=255)
                                                                                                                            gctgcGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGa                           <  1:4601592/150‑1 (MQ=255)
                                                                                                                               gcgcgcATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGt                        >  1:859661/1‑150 (MQ=255)
                                                                                                                                  cgcATTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCaa                     <  1:462484/150‑1 (MQ=255)
                                                                                                                                   gcATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAAc                    >  2:554003/1‑150 (MQ=255)
                                                                                                                                     aTTTGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGc                  >  2:3291727/1‑150 (MQ=255)
                                                                                                                                       ttGCCCGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCg                >  1:2660266/1‑150 (MQ=255)
                                                                                                                                           ccGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTcc            <  2:826862/150‑1 (MQ=255)
                                                                                                                                            cGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCg           >  1:2756565/1‑150 (MQ=255)
                                                                                                                                            cGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCg           >  2:685234/1‑150 (MQ=255)
                                                                                                                                            cGCCTTCTTATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCg           <  2:2686723/150‑1 (MQ=255)
                                                                                                                                             gCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCgg          >  2:1102586/1‑150 (MQ=255)
                                                                                                                                               cttcttATCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGgcg        >  1:237150/1‑150 (MQ=255)
                                                                                                                                                 tcttatCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGcgcg      <  1:3376566/150‑1 (MQ=255)
                                                                                                                                                 tcttatCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGcgcg      <  2:295115/150‑1 (MQ=255)
                                                                                                                                                  cttatCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGt     >  1:2547488/1‑150 (MQ=255)
                                                                                                                                                   ttctCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTa    >  1:1983536/1‑150 (MQ=255)
                                                                                                                                                   ttctCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTa    <  2:3204421/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCGACTGTGGCTAACGCAGTTGTCGCTTGGGCAGAACAAAATCTGAAAATGATGTCAGGCGTTATCTTTTCTTTCTTTAGCGCCTAAAATCGACCTCCCCCCTTTCGTTAAGGATAACGACCATGCTGCGCGCATTTGCCCGCCTTCTTCTCCGTATTTGTTTTTCTCGCCGGACTCTAAAGATTGCCTGCTTACTGTTACTTGTGGCAGGAGCGACCATCCTTATCGCCGATCGCGTAATGGTCAACGCCAGCAAACAGCTGACCTGGAGCGATGTCAACGCCGTTCCGGCGCGTAAC  >  CP009273/3229777‑3230075

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A