Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,230,857 C→G 8.3% A63G (GCC→GGC)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,230,8570CG 8.3% 933.4 / 67.8 254A63G (GCC→GGC) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (128/105);  new base G (10/11);  total (138/116)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 258 total aligned reads displayed.

TCGCCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAA  >  CP009273/3230708‑3231006
                                                                                                                                                     |                                                                                                                                                     
      gTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGa                                                                                                                                                 <  1:3239538/150‑1 (MQ=255)
        tcgtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGAcg                                                                                                                                               <  1:4533468/150‑1 (MQ=255)
         cgtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGAcgc                                                                                                                                              >  1:1981364/1‑150 (MQ=255)
          gtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGAcgcg                                                                                                                                             >  1:3916148/1‑150 (MQ=255)
            cgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgt                                                                                                                                           >  2:1240307/1‑150 (MQ=255)
             gAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGgtg                                                                                                                                          <  2:3618033/150‑1 (MQ=255)
                 ccgccCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGtata                                                                                                                                      >  1:445514/1‑150 (MQ=255)
                        tGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAg                                                                                                                               >  2:2196769/1‑150 (MQ=255)
                         gagaGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGc                                                                                                                              >  1:3801484/1‑150 (MQ=255)
                         gagaGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGc                                                                                                                              >  1:1928327/1‑150 (MQ=255)
                            agCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCg                                                                                                                           >  1:2053523/1‑150 (MQ=255)
                               ggTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAAt                                                                                                                        <  1:967967/150‑1 (MQ=255)
                                gTAAATCCACGTGAACCGCCGTATAGTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAtt                                                                                                                       <  1:1038363/150‑1 (MQ=255)
                                gTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAtt                                                                                                                       >  1:1816621/1‑150 (MQ=255)
                                 tAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTc                                                                                                                      >  2:3091954/1‑150 (MQ=255)
                                 tAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTc                                                                                                                      <  1:1328529/150‑1 (MQ=255)
                                  aaaTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTcc                                                                                                                     <  2:2480846/150‑1 (MQ=255)
                                  aaaTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTcc                                                                                                                     >  1:622425/1‑150 (MQ=255)
                                  aaaTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTcc                                                                                                                     <  2:215863/150‑1 (MQ=255)
                                    aTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCt                                                                                                                   <  1:2414645/150‑1 (MQ=255)
                                    aTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCt                                                                                                                   >  2:3812867/1‑150 (MQ=255)
                                     tACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTg                                                                                                                  >  2:4333908/1‑150 (MQ=255)
                                       cAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCa                                                                                                                <  2:1502385/150‑1 (MQ=255)
                                         aGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                              <  2:1246103/150‑1 (MQ=255)
                                          gTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttt                                                                                                             >  2:1317629/1‑150 (MQ=255)
                                            tAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttttt                                                                                                           >  2:3669864/1‑150 (MQ=255)
                                             aaCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTc                                                                                                          <  1:1722424/150‑1 (MQ=255)
                                              aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTcc                                                                                                         >  1:3804551/1‑150 (MQ=255)
                                                 gccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAg                                                                                                      >  2:792021/1‑150 (MQ=255)
                                                  ccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGa                                                                                                     >  2:2869119/1‑150 (MQ=255)
                                                     tataTTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGacac                                                                                                  >  2:4471823/1‑150 (MQ=255)
                                                        atTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAAc                                                                                               >  2:2201534/1‑150 (MQ=255)
                                                          tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                             <  2:4532629/150‑1 (MQ=255)
                                                          tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                             >  2:4362446/1‑150 (MQ=255)
                                                          tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                             >  1:4070287/1‑150 (MQ=255)
                                                              gCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTcc                                                                                         <  1:615680/150‑1 (MQ=255)
                                                                aGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCtt                                                                                       >  1:3358545/1‑150 (MQ=255)
                                                                    ttGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGcc                                                                                   <  1:42258/150‑1 (MQ=255)
                                                                      gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCAt                                                                                 >  2:3058464/1‑150 (MQ=255)
                                                                      gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCAt                                                                                 >  2:1650419/1‑150 (MQ=255)
                                                                       aaCAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATa                                                                                >  1:1455096/1‑150 (MQ=255)
                                                                        aCAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaa                                                                               <  1:229928/150‑1 (MQ=255)
                                                                        aCAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaa                                                                               <  1:3091954/150‑1 (MQ=255)
                                                                         cAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCACAGACACAAATTTTACTTAGCCATaaa                                                                              >  1:3610061/1‑150 (MQ=255)
                                                                          aGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                             <  2:4185366/150‑1 (MQ=255)
                                                                            gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAAtt                                                                           <  2:3932435/150‑1 (MQ=255)
                                                                            gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAAtt                                                                           <  1:1327949/150‑1 (MQ=255)
                                                                               cAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAt                                                                        <  2:578705/150‑1 (MQ=255)
                                                                               cAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAt                                                                        >  2:184263/1‑150 (MQ=255)
                                                                                aGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtg                                                                       <  2:2446882/150‑1 (MQ=255)
                                                                                aGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtg                                                                       <  2:183266/150‑1 (MQ=255)
                                                                                aGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtg                                                                       >  2:1570908/1‑150 (MQ=255)
                                                                                  cACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgtg                                                                     >  1:1109758/1‑150 (MQ=255)
                                                                                   aCTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                                    >  2:860607/1‑150 (MQ=255)
                                                                                   aCTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                                    >  1:251386/1‑150 (MQ=255)
                                                                                   aCTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                                    >  2:3975567/1‑150 (MQ=255)
                                                                                   aCTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                                    >  1:2931844/1‑150 (MQ=255)
                                                                                    cTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAt                                                                   <  2:2436714/150‑1 (MQ=255)
                                                                                      tCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAttt                                                                 <  2:3670171/150‑1 (MQ=255)
                                                                                       cGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTg                                                                <  1:748619/150‑1 (MQ=255)
                                                                                       cGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTg                                                                >  2:3046503/1‑150 (MQ=255)
                                                                                         ccAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                              <  2:1099781/150‑1 (MQ=255)
                                                                                          cAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCga                                                             >  1:4505407/1‑150 (MQ=255)
                                                                                           aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgag                                                            >  2:1446695/1‑150 (MQ=255)
                                                                                           aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgag                                                            >  1:520708/1‑150 (MQ=255)
                                                                                              gATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                         <  1:4435355/150‑1 (MQ=255)
                                                                                                      tGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGc                                                 >  2:2795559/1‑150 (MQ=255)
                                                                                                      tGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCACTGGc                                                 <  2:3152571/150‑1 (MQ=255)
                                                                                                           aGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGa                                            >  1:1366523/1‑150 (MQ=255)
                                                                                                              aTCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATc                                         >  1:1276672/1‑150 (MQ=255)
                                                                                                               tCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCt                                        <  2:3450636/150‑1 (MQ=255)
                                                                                                                      aCCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGaa                                 >  2:2356272/1‑150 (MQ=255)
                                                                                                                         tCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTg                              >  1:4482903/1‑150 (MQ=255)
                                                                                                                         tCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTg                              <  1:2616363/150‑1 (MQ=255)
                                                                                                                         tCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTg                              <  1:352337/150‑1 (MQ=255)
                                                                                                                          cGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTgg                             <  1:3710780/150‑1 (MQ=255)
                                                                                                                            cTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                           >  1:3875763/1‑150 (MQ=255)
                                                                                                                            cTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                           <  2:4284668/150‑1 (MQ=255)
                                                                                                                             tGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTGTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATg                          <  2:1953875/150‑1 (MQ=255)
                                                                                                                              ggAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgc                         >  2:545963/1‑150 (MQ=255)
                                                                                                                                 aGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgccgc                      >  2:4106552/1‑150 (MQ=255)
                                                                                                                                   cGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCa                    >  1:2446769/1‑150 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCCTAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                   >  1:4233510/1‑150 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                   >  1:3021051/1‑150 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                   >  1:2182884/1‑150 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                   <  2:2914150/150‑1 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                   <  2:2510416/150‑1 (MQ=255)
                                                                                                                                       ggCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGc                >  1:1643436/1‑150 (MQ=255)
                                                                                                                                         cGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCt              <  2:707205/150‑1 (MQ=255)
                                                                                                                                          ggAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg             >  1:3089340/1‑150 (MQ=255)
                                                                                                                                          ggAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg             >  2:1213071/1‑150 (MQ=255)
                                                                                                                                          ggAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg             <  2:1480981/150‑1 (MQ=255)
                                                                                                                                           gAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtgt            >  2:4372513/1‑150 (MQ=255)
                                                                                                                                            aaaGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtgtg           <  2:1051274/150‑1 (MQ=255)
                                                                                                                                             aaGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCCTTGCCTGTgc          >  2:2719027/1‑150 (MQ=255)
                                                                                                                                              aGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTgcg         >  2:4427569/1‑150 (MQ=255)
                                                                                                                                                cGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGcgcg       >  2:4077563/1‑150 (MQ=255)
                                                                                                                                                 gATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCgg      >  2:2954500/1‑150 (MQ=255)
                                                                                                                                                   tGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGa    <  2:3627539/150‑1 (MQ=255)
                                                                                                                                                     ccATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGaaa  >  2:1758178/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TCGCCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAAA  >  CP009273/3230708‑3231006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A