Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F181 I0 R1 1262 323.1 10297870 97.5% 10040423 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,230,857 C→G 44.7% A63G (GCC→GGC)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,230,8570CG44.7% 188.7 / inf 371A63G (GCC→GGC) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (96/109);  new base G (83/82);  total (179/192)
Fisher's exact test for biased strand distribution p-value = 5.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 375 total aligned reads displayed.

TCGCCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAA  >  CP009273/3230708‑3231005
                                                                                                                                                     |                                                                                                                                                    
tCGCCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGc                                                                                                                                                      >  1:360151/1‑150 (MQ=255)
    cAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCAtt                                                                                                                                                  <  2:1120616/150‑1 (MQ=255)
    cAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCAtt                                                                                                                                                  <  1:3748912/150‑1 (MQ=255)
     aGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTg                                                                                                                                                 >  2:2402603/1‑150 (MQ=255)
        tcgtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGAcg                                                                                                                                              <  2:4511667/150‑1 (MQ=255)
          gtcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGAcgcg                                                                                                                                            <  2:660041/150‑1 (MQ=255)
            cgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGgt                                                                                                                                          <  2:1750214/150‑1 (MQ=255)
             gAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgtg                                                                                                                                         >  2:3977485/1‑150 (MQ=255)
                 ccgccCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGtata                                                                                                                                     <  2:139286/150‑1 (MQ=255)
                    cccATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTg                                                                                                                                  <  1:3693910/150‑1 (MQ=255)
                       aTGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCAGTGACGCGGGGTATATTGCCa                                                                                                                               >  2:1415493/1‑150 (MQ=255)
                        tGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAg                                                                                                                              >  1:1785267/1‑150 (MQ=255)
                         gagaGCGGTAAATACAAGTTAACCGCCGTACATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGc                                                                                                                             <  1:2678224/150‑1 (MQ=255)
                           gagCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGccc                                                                                                                           <  1:2185113/150‑1 (MQ=255)
                           gagCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGccc                                                                                                                           <  1:4198372/150‑1 (MQ=255)
                            agCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCg                                                                                                                          <  2:1012149/150‑1 (MQ=255)
                              cGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGaa                                                                                                                        <  1:2237404/150‑1 (MQ=255)
                              cGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGaa                                                                                                                        >  1:62444/1‑150 (MQ=255)
                               ggTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAt                                                                                                                       >  1:4022531/1‑150 (MQ=255)
                               ggTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAt                                                                                                                       >  1:1122210/1‑150 (MQ=255)
                                gTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAtt                                                                                                                      <  1:4394950/150‑1 (MQ=255)
                                gTAAAGACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACCGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTTCCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAtt                                                                                                                      <  1:5103213/150‑1 (MQ=255)
                                         aGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                             >  1:2753516/1‑150 (MQ=255)
                                            tAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAttttt                                                                                                          <  1:3002139/150‑1 (MQ=255)
                                              aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTcc                                                                                                        >  1:2095622/1‑150 (MQ=255)
                                              aCCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTcc                                                                                                        <  1:2620603/150‑1 (MQ=255)
                                                cgccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCa                                                                                                      <  2:1065/150‑1 (MQ=255)
                                                   cgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGac                                                                                                   <  1:4777827/150‑1 (MQ=255)
                                                    gTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGaca                                                                                                  <  2:4716998/150‑1 (MQ=255)
                                                      atatTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGAcaca                                                                                                >  2:4268177/1‑150 (MQ=255)
                                                         ttCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACt                                                                                             <  2:4048280/150‑1 (MQ=255)
                                                         ttCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACt                                                                                             <  2:2867380/150‑1 (MQ=255)
                                                           cccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACttt                                                                                           <  1:4951844/150‑1 (MQ=255)
                                                           cccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACttt                                                                                           >  1:1939727/1‑150 (MQ=255)
                                                           cccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACttg                                                                                           >  2:3004583/1‑149 (MQ=255)
                                                             cGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                         <  1:1637132/150‑1 (MQ=255)
                                                               cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                       <  1:4837305/150‑1 (MQ=255)
                                                               cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCt                                                                                       >  2:228439/1‑150 (MQ=255)
                                                                 gCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTa                                                                                     <  1:3250718/150‑1 (MQ=255)
                                                                  ccTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAg                                                                                    <  2:4447808/150‑1 (MQ=255)
                                                                    ttGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGcc                                                                                  >  2:4922277/1‑150 (MQ=255)
                                                                     tGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCa                                                                                 <  2:5066162/150‑1 (MQ=255)
                                                                     tGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCa                                                                                 <  2:1172138/150‑1 (MQ=255)
                                                                     tGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCa                                                                                 <  2:5066114/150‑1 (MQ=255)
                                                                      gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCAt                                                                                >  1:4081405/1‑150 (MQ=255)
                                                                         cAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGACAGCGATGCCATTGACGCGGGGGATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCagcaa                                                                             >  2:2693306/1‑146 (MQ=255)
                                                                         cAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaa                                                                             <  2:2580958/150‑1 (MQ=255)
                                                                          aGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                            >  2:2740183/1‑150 (MQ=255)
                                                                           ggCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAAt                                                                           <  1:2354486/150‑1 (MQ=255)
                                                                             cccAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTGGCCATAAAATTa                                                                         >  1:1026252/1‑150 (MQ=255)
                                                                                 gCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgt                                                                     <  2:4804516/150‑1 (MQ=255)
                                                                                   aCTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGa                                                                   >  1:1528719/1‑150 (MQ=255)
                                                                                     ttCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAtt                                                                 <  2:3582144/150‑1 (MQ=255)
                                                                                     ttCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAtt                                                                 <  2:3509919/150‑1 (MQ=255)
                                                                                      tCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAttt                                                                <  2:4758848/150‑1 (MQ=255)
                                                                                         ccAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                             <  1:3795975/150‑1 (MQ=255)
                                                                                         ccAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                             >  1:856250/1‑150 (MQ=255)
                                                                                             tGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaa                                                         <  2:1331509/150‑1 (MQ=255)
                                                                                             tGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaa                                                         <  2:2485454/150‑1 (MQ=255)
                                                                                              gATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAAtt                                                                          <  1:1285364/132‑1 (MQ=255)
                                                                                                 ttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCg                                                     >  2:867300/1‑150 (MQ=255)
                                                                                                 ttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCg                                                     >  1:4126749/1‑150 (MQ=255)
                                                                                                  tttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGc                                                    >  2:3032130/1‑150 (MQ=255)
                                                                                                  tttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGc                                                    >  2:548877/1‑150 (MQ=255)
                                                                                                   ttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCt                                                   >  1:611795/1‑150 (MQ=255)
                                                                                                     cTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTgg                                                 >  1:3953769/1‑150 (MQ=255)
                                                                                                        tCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGt                                              >  1:3445859/1‑150 (MQ=255)
                                                                                                         cGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTc                                             <  1:2377645/150‑1 (MQ=255)
                                                                                                             cATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAAt                                         <  2:2136948/150‑1 (MQ=255)
                                                                                                              aTCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATc                                        >  2:1581080/1‑150 (MQ=255)
                                                                                                              aTCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATc                                        >  1:2669964/1‑150 (MQ=255)
                                                                                                              aTCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATc                                        >  2:2071448/1‑150 (MQ=255)
                                                                                                               tCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCt                                       <  2:1544837/150‑1 (MQ=255)
                                                                                                                   tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCg                                   >  1:2526203/1‑150 (MQ=255)
                                                                                                                   tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCg                                   <  2:1077436/150‑1 (MQ=255)
                                                                                                                      aCCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGaa                                <  1:3168132/150‑1 (MQ=255)
                                                                                                                       ccTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAg                               >  1:2884174/1‑150 (MQ=255)
                                                                                                                       ccTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAg                               <  2:4640753/150‑1 (MQ=255)
                                                                                                                       ccTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAg                               <  2:590697/150‑1 (MQ=255)
                                                                                                                        cTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGt                              >  1:636243/1‑150 (MQ=255)
                                                                                                                         tCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTg                             <  2:4225124/150‑1 (MQ=255)
                                                                                                                           gCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGa                           >  2:1507229/1‑150 (MQ=255)
                                                                                                                            cTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                          <  2:2322102/150‑1 (MQ=255)
                                                                                                                             tGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATg                         <  1:3111043/150‑1 (MQ=255)
                                                                                                                               gAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgcc                       <  1:931089/150‑1 (MQ=255)
                                                                                                                                  gCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgccgcc                    >  2:330346/1‑150 (MQ=255)
                                                                                                                                    gATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt                  >  2:1927750/1‑150 (MQ=255)
                                                                                                                                     aTGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAtt                 >  1:1433817/1‑150 (MQ=255)
                                                                                                                                         cGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCt             <  2:4360077/150‑1 (MQ=255)
                                                                                                                                          ggAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg            >  2:764922/1‑150 (MQ=255)
                                                                                                                                           gAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtgt           <  1:1013657/150‑1 (MQ=255)
                                                                                                                                            acaGCGAGGGCATTGCCGCGGTGGATCGGGCCAGCCCGACGTCCCGGCAGTTGTCCCCGCCACACCGGGTCCTGAGCCATAAACTGCCGGGGCTTGGCGAGACCCCGCTGGCGTCGCATCTGGCGGAAGGGGATGCCGCCATTGCCtgtg          <  2:3389662/148‑1 (MQ=255)
                                                                                                                                                cGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGcgcg      >  1:3504524/1‑150 (MQ=255)
                                                                                                                                                cGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGcgcg      >  1:75936/1‑150 (MQ=255)
                                                                                                                                                cGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGcgcg      >  2:5104985/1‑150 (MQ=255)
                                                                                                                                                 gATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCgg     >  2:1887652/1‑150 (MQ=255)
                                                                                                                                                 caTGCCATTGACGCGGTCTATAATGCCAGACCGAATTCCCTGCATTTTTCCAAGACACAACTTTACCTTAGAAATAAAATTAATGTGATATGAGAGAAAACGATGGCGTCGAATCTGGCGGAAGTGTATGCCGCAATTGCATGTGCGCgg     >  2:3729941/2‑150 (MQ=255)
                                                                                                                                                  aTGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCCTAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCggg    <  2:1837148/150‑1 (MQ=255)
                                                                                                                                                    gCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGaa  >  2:4103412/1‑150 (MQ=255)
                                                                                                                                                    gCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGaa  >  2:1301269/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGCCAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAA  >  CP009273/3230708‑3231005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A