Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,230,857 C→G 100% A63G (GCC→GGC)  ygjR → putative NAD(P)‑dependent dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,230,8570CG97.0% 918.3 / 14.0 234A63G (GCC→GGC) ygjRputative NAD(P)‑dependent dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base G (112/115);  total (114/120)
Fisher's exact test for biased strand distribution p-value = 4.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 234 total aligned reads displayed.

CAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCG  >  CP009273/3230712‑3230999
                                                                                                                                                 |                                                                                                                                              
cAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCa                                                                                                                                              <  1:442646/148‑1 (MQ=255)
   ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                           >  1:1206624/1‑148 (MQ=255)
   ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                           >  2:688818/1‑148 (MQ=255)
   ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                           <  1:2842516/148‑1 (MQ=255)
   ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                           <  2:2935372/148‑1 (MQ=255)
   ttCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAACACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTg                                                                                                                                           >  1:3376360/1‑148 (MQ=255)
       tcgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGAcgc                                                                                                                                       >  2:612133/1‑148 (MQ=255)
        cgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGAcgcg                                                                                                                                      >  1:3156311/1‑148 (MQ=255)
        cgAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGAcgcg                                                                                                                                      <  1:3187767/148‑1 (MQ=255)
          aGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgt                                                                                                                                    <  2:1472772/148‑1 (MQ=255)
          aGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgt                                                                                                                                    <  1:2619984/148‑1 (MQ=255)
           ggCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgtg                                                                                                                                   >  1:1530756/1‑148 (MQ=255)
           ggCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgtg                                                                                                                                   >  2:685186/1‑148 (MQ=255)
           ggCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCAAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGgtg                                                                                                                                   >  2:3090037/1‑148 (MQ=255)
            gccgccCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGgtgt                                                                                                                                  <  2:795225/148‑1 (MQ=255)
               gccCATGAGAGCGGTAAATACACGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGtata                                                                                                                               <  1:1708689/148‑1 (MQ=255)
                cccATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTatat                                                                                                                              <  2:1516651/148‑1 (MQ=255)
                  cATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTg                                                                                                                            >  2:2010548/1‑148 (MQ=255)
                  cATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTg                                                                                                                            <  1:1574339/148‑1 (MQ=255)
                    tGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGcc                                                                                                                          <  1:501065/148‑1 (MQ=255)
                       gagCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGGCGCGGTGTATATTGCCAGc                                                                                                                       >  1:3456398/1‑148 (MQ=255)
                       gagCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGc                                                                                                                       >  2:185296/1‑148 (MQ=255)
                        agCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCTTTGACGCGGTGTATATTGCCAGcc                                                                                                                      <  2:939430/148‑1 (MQ=255)
                            gTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGaa                                                                                                                  >  2:445098/1‑148 (MQ=255)
                             tAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAt                                                                                                                 >  1:2523933/1‑148 (MQ=255)
                             tAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAt                                                                                                                 >  1:350113/1‑148 (MQ=255)
                               aaTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTc                                                                                                               <  1:1347875/148‑1 (MQ=255)
                                aTACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATCTTGCCAGCCCGAATTcc                                                                                                              >  2:2292578/1‑148 (MQ=255)
                                  aCAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCt                                                                                                            >  1:425330/1‑148 (MQ=255)
                                     aGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCa                                                                                                         <  2:1206624/148‑1 (MQ=255)
                                     aGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCa                                                                                                         >  1:1049039/1‑148 (MQ=255)
                                     aGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCa                                                                                                         >  1:2828399/1‑148 (MQ=255)
                                       ttAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                       >  2:19007/1‑148 (MQ=255)
                                       ttAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAtt                                                                                                       >  2:2594363/1‑148 (MQ=255)
                                             gccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTccc                                                                                                 <  1:1453160/148‑1 (MQ=255)
                                             gccgTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTccc                                                                                                 <  1:2811165/148‑1 (MQ=255)
                                             gccgTATAGTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTccc                                                                                                 <  1:799934/148‑1 (MQ=255)
                                                  atatTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGaca                                                                                            >  1:704765/1‑148 (MQ=255)
                                                    atTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGAcaca                                                                                          >  1:57178/1‑148 (MQ=255)
                                                      tCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAAc                                                                                        <  1:712198/148‑1 (MQ=255)
                                                       cccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACt                                                                                       <  2:344167/148‑1 (MQ=255)
                                                        ccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                      <  2:352369/148‑1 (MQ=255)
                                                        ccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                      <  2:1075467/148‑1 (MQ=255)
                                                        ccGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACtt                                                                                      <  1:1298866/148‑1 (MQ=255)
                                                         cGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACttt                                                                                     <  1:855530/148‑1 (MQ=255)
                                                           cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                   <  1:2507079/148‑1 (MQ=255)
                                                           cAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTc                                                                                   <  1:1994054/148‑1 (MQ=255)
                                                            aGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTcc                                                                                  <  1:2718938/148‑1 (MQ=255)
                                                                  gAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGcc                                                                            <  2:1111617/148‑1 (MQ=255)
                                                                        gCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATaaaa                                                                      <  2:824148/148‑1 (MQ=255)
                                                                            aGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTaa                                                                  >  2:2317709/1‑148 (MQ=255)
                                                                             gCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATTGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAt                                                                 <  2:2748022/148‑1 (MQ=255)
                                                                              cACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtg                                                                >  2:2404302/1‑148 (MQ=255)
                                                                                cTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgtg                                                              <  1:2686809/148‑1 (MQ=255)
                                                                                cTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgtg                                                              <  2:1065324/148‑1 (MQ=255)
                                                                                cTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAAtgtg                                                              <  2:138511/148‑1 (MQ=255)
                                                                                    gCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAttt                                                          >  2:1208252/1‑148 (MQ=255)
                                                                                     caattGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTATCCATAAAATTAATGTGATTTg                                                         <  1:827307/144‑1 (MQ=255)
                                                                                       aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                       <  1:1237608/148‑1 (MQ=255)
                                                                                       aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                       >  2:2486380/1‑148 (MQ=255)
                                                                                       aaTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCg                                                       >  1:3285967/1‑148 (MQ=255)
                                                                                        aTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCga                                                      >  2:2513575/1‑148 (MQ=255)
                                                                                         tGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgag                                                     >  1:265189/1‑148 (MQ=255)
                                                                                         tGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgag                                                     >  2:1669109/1‑148 (MQ=255)
                                                                                         tGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgag                                                     >  1:1858149/1‑148 (MQ=255)
                                                                                          gATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgaga                                                    <  1:1708626/148‑1 (MQ=255)
                                                                                          gATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgaga                                                    <  2:3005878/148‑1 (MQ=255)
                                                                                          gATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCgaga                                                    <  2:1226187/148‑1 (MQ=255)
                                                                                            tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                  >  1:3363364/1‑148 (MQ=255)
                                                                                            tttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGaaa                                                  <  2:1495083/148‑1 (MQ=255)
                                                                                             ttttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAAc                                                 >  1:2383560/1‑148 (MQ=255)
                                                                                               ttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCg                                               <  1:2111577/148‑1 (MQ=255)
                                                                                               ttCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCg                                               <  1:3422660/148‑1 (MQ=255)
                                                                                                tCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGc                                              <  2:3398801/148‑1 (MQ=255)
                                                                                                      gAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGt                                        <  1:1106357/148‑1 (MQ=255)
                                                                                                       aGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTc                                       >  2:2042288/1‑148 (MQ=255)
                                                                                                         cATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGa                                     >  1:3098168/1‑148 (MQ=255)
                                                                                                         cATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGa                                     >  1:1323729/1‑148 (MQ=255)
                                                                                                          aTCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGaa                                    >  2:1165792/1‑148 (MQ=255)
                                                                                                            cTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATc                                  >  1:2362779/1‑148 (MQ=255)
                                                                                                              gTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTg                                >  2:2811002/1‑148 (MQ=255)
                                                                                                               tttACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTgg                               <  2:327185/148‑1 (MQ=255)
                                                                                                                   ccTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGa                           >  2:2846620/1‑148 (MQ=255)
                                                                                                                    cTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGaa                          <  1:685536/148‑1 (MQ=255)
                                                                                                                    cTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGaa                          <  2:3202331/148‑1 (MQ=255)
                                                                                                                     tCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAg                         <  2:1068926/148‑1 (MQ=255)
                                                                                                                      cGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGt                        >  1:402945/1‑148 (MQ=255)
                                                                                                                      cGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGt                        <  1:2764556/148‑1 (MQ=255)
                                                                                                                       gCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTg                       <  2:883007/148‑1 (MQ=255)
                                                                                                                          ggAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAt                    >  1:168951/1‑148 (MQ=255)
                                                                                                                              gCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGAGGAAGTGGATgccg                >  1:2248526/1‑148 (MQ=255)
                                                                                                                               cGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgccgc               >  2:3258572/1‑148 (MQ=255)
                                                                                                                               cGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATgccgc               >  2:2957997/1‑148 (MQ=255)
                                                                                                                                 aTGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCa             <  2:2930562/148‑1 (MQ=255)
                                                                                                                                  tGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCAt            <  2:907919/148‑1 (MQ=255)
                                                                                                                                    gCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTg          <  2:704865/148‑1 (MQ=255)
                                                                                                                                     cGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGc         <  2:639592/148‑1 (MQ=255)
                                                                                                                                        aaaGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg      <  1:644756/148‑1 (MQ=255)
                                                                                                                                        aaaGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtg      >  1:1031014/1‑148 (MQ=255)
                                                                                                                                         aaGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCtgt     <  2:617083/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                              
CAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAATGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCG  >  CP009273/3230712‑3230999

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAA  >  CP009273/3230712‑3231005
                                                                                                                                                  |                                                                                                                                                    
CAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATT                                                                                                                                                  <  SRR19973066.446067/150‑1 (MQ=60)
 AGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTG                                                                                                                                                 >  SRR19973066.1215042/1‑150 (MQ=60)
 AGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTG                                                                                                                                                 >  SRR19973066.1215056/1‑150 (MQ=60)
 AGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAACACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTG                                                                                                                                                 >  SRR19973066.3398875/1‑150 (MQ=60)
  GTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGA                                                                                                                                                >  SRR19973066.801066/1‑150 (MQ=60)
   TTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGAC                                                                                                                                               <  SRR19973066.2861618/150‑1 (MQ=60)
      GTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCG                                                                                                                                            >  SRR19973066.3177349/1‑150 (MQ=60)
        CGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGT                                                                                                                                          <  SRR19973066.3207928/150‑1 (MQ=60)
        CGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGT                                                                                                                                          <  SRR19973066.3208962/150‑1 (MQ=60)
        CGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGT                                                                                                                                          <  SRR19973066.1811895/150‑1 (MQ=60)
         GAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTG                                                                                                                                         >  SRR19973066.131194/1‑150 (MQ=60)
         GAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTG                                                                                                                                         >  SRR19973066.1542354/1‑150 (MQ=60)
         GAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTG                                                                                                                                         >  SRR19973066.1541634/1‑150 (MQ=60)
          AGGCCGCCCATGAGCGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGT                                                                                                                                        <  SRR19973066.839016/150‑1 (MQ=60)
          AGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGT                                                                                                                                        <  SRR19973066.2637664/150‑1 (MQ=60)
          AGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGT                                                                                                                                        <  SRR19973066.2637736/150‑1 (MQ=60)
            GCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTAT                                                                                                                                      <  SRR19973066.2378261/150‑1 (MQ=60)
               GCCCATGAGAGCGGTAAATACACGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATT                                                                                                                                   <  SRR19973066.1721087/150‑1 (MQ=60)
               GCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATT                                                                                                                                   <  SRR19973066.1721080/150‑1 (MQ=60)
                 CCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGC                                                                                                                                 >  SRR19973066.478478/1‑150 (MQ=60)
                  CATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCC                                                                                                                                <  SRR19973066.1585723/150‑1 (MQ=60)
                  CATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCC                                                                                                                                <  SRR19973066.1585686/150‑1 (MQ=60)
                    TGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAG                                                                                                                              <  SRR19973066.504789/150‑1 (MQ=60)
                     GAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGGCGCGGTGTATATTGCCAGC                                                                                                                             >  SRR19973066.3479509/1‑150 (MQ=60)
                      AGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCC                                                                                                                            >  SRR19973066.2165672/1‑150 (MQ=60)
                       GAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCC                                                                                                                           >  SRR19973066.2785608/1‑150 (MQ=60)
                           GGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAT                                                                                                                       >  SRR19973066.2541101/1‑150 (MQ=60)
                           GGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAT                                                                                                                       >  SRR19973066.1413315/1‑150 (MQ=60)
                           GGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAT                                                                                                                       >  SRR19973066.352845/1‑150 (MQ=60)
                           GGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAAT                                                                                                                       >  SRR19973066.351722/1‑150 (MQ=60)
                              AAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCC                                                                                                                    >  SRR19973066.3414963/1‑150 (MQ=60)
                               AATACACGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCC                                                                                                                   <  SRR19973066.3267879/150‑1 (MQ=60)
                               AATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCC                                                                                                                   <  SRR19973066.1357651/150‑1 (MQ=60)
                               AATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCC                                                                                                                   <  SRR19973066.3267864/150‑1 (MQ=60)
                                ATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCT                                                                                                                  >  SRR19973066.428635/1‑150 (MQ=60)
                                ATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCT                                                                                                                  <  SRR19973066.693884/150‑1 (MQ=60)
                                ATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCT                                                                                                                  >  SRR19973066.428629/1‑150 (MQ=60)
                                ATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCT                                                                                                                  >  SRR19973066.428624/1‑150 (MQ=60)
                                 accctgttctatggctccaATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTG                                                                                                                 <  SRR19973066.2362744/131‑1 (MQ=60)
                                 TACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTG                                                                                                                 >  SRR19973066.2435045/1‑150 (MQ=60)
                                   CAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCA                                                                                                               >  SRR19973066.2847434/1‑150 (MQ=60)
                                   CAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCA                                                                                                               >  SRR19973066.2847401/1‑150 (MQ=60)
                                   CAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCA                                                                                                               >  SRR19973066.1056749/1‑150 (MQ=60)
                                    AAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCAT                                                                                                              >  SRR19973066.3421494/1‑150 (MQ=60)
                                      GTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTT                                                                                                            <  SRR19973066.2701865/150‑1 (MQ=60)
                                      GTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTT                                                                                                            <  SRR19973066.849006/150‑1 (MQ=60)
                                           CCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCC                                                                                                       >  SRR19973066.2610583/1‑150 (MQ=60)
                                            CGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCA                                                                                                      <  SRR19973066.197955/150‑1 (MQ=60)
                                            CGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCA                                                                                                      <  SRR19973066.678174/150‑1 (MQ=60)
                                            CGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCA                                                                                                      <  SRR19973066.2778568/150‑1 (MQ=60)
                                             GCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAG                                                                                                     <  SRR19973066.1463467/150‑1 (MQ=60)
                                             GCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAG                                                                                                     <  SRR19973066.2830074/150‑1 (MQ=60)
                                             GCCGTATAGTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAG                                                                                                     <  SRR19973066.805810/150‑1 (MQ=60)
                                                GTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACA                                                                                                  >  SRR19973066.710070/1‑150 (MQ=60)
                                                GTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACA                                                                                                  >  SRR19973066.709967/1‑150 (MQ=60)
                                                  ATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACA                                                                                                >  SRR19973066.57887/1‑150 (MQ=60)
                                                   TATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAA                                                                                               >  SRR19973066.1048488/1‑150 (MQ=60)
                                                     TTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACT                                                                                             <  SRR19973066.272341/150‑1 (MQ=60)
                                                      TCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTT                                                                                            <  SRR19973066.717473/150‑1 (MQ=60)
                                                       CCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTT                                                                                           >  SRR19973066.53253/1‑150 (MQ=60)
                                                        CCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTT                                                                                          <  SRR19973066.1160271/150‑1 (MQ=60)
                                                        CCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTT                                                                                          <  SRR19973066.1308277/150‑1 (MQ=60)
                                                         CGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTC                                                                                         <  SRR19973066.60893/150‑1 (MQ=60)
                                                         CGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTC                                                                                         <  SRR19973066.861705/150‑1 (MQ=60)
                                                         CGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTC                                                                                         <  SRR19973066.1100420/150‑1 (MQ=60)
                                                           CAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCT                                                                                       <  SRR19973066.2008083/150‑1 (MQ=60)
                                                           CAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCT                                                                                       <  SRR19973066.2524152/150‑1 (MQ=60)
                                                            AGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTT                                                                                      <  SRR19973066.2737220/150‑1 (MQ=60)
                                                              CCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAG                                                                                    <  SRR19973066.3141419/150‑1 (MQ=60)
                                                                  GAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCAT                                                                                <  SRR19973066.2111825/150‑1 (MQ=60)
                                                                        GCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATT                                                                          <  SRR19973066.2837569/150‑1 (MQ=60)
                                                                                CTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAT                                                                  <  SRR19973066.2704732/150‑1 (MQ=60)
                                                                                CTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGAT                                                                  >  SRR19973066.2530532/1‑150 (MQ=60)
                                                                                   CGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTG                                                               <  SRR19973066.1987431/150‑1 (MQ=60)
                                                                                   CGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTG                                                               >  SRR19973066.3585149/1‑150 (MQ=60)
                                                                                    GCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGC                                                              >  SRR19973066.1477889/1‑150 (MQ=60)
                                                                                    GCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGC                                                              <  SRR19973066.2691458/150‑1 (MQ=60)
                                                                                     CCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCG                                                             >  SRR19973066.3307612/1‑150 (MQ=60)
                                                                                      CAATTGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTATCCATAAAATTAATGTGATTTGCG                                                             <  SRR19973066.833342/150‑1 (MQ=60)
                                                                                       AA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAG                                                           <  SRR19973066.1246442/150‑1 (MQ=60)
                                                                                       AA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAG                                                           >  SRR19973066.1871435/1‑150 (MQ=60)
                                                                                       AA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAG                                                           >  SRR19973066.267175/1‑150 (MQ=60)
                                                                                       AA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAG                                                           <  SRR19973066.1246428/150‑1 (MQ=60)
                                                                                           GATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAA                                                        >  SRR19973066.3385786/1‑150 (MQ=60)
                                                                                           GATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAA                                                        >  SRR19973066.3385767/1‑150 (MQ=60)
                                                                                           GATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAA                                                        <  SRR19973066.1721024/150‑1 (MQ=60)
                                                                                            ATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAAC                                                       >  SRR19973066.2399633/1‑150 (MQ=60)
                                                                                                TTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCT                                                   <  SRR19973066.3394775/150‑1 (MQ=60)
                                                                                                TTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCT                                                   <  SRR19973066.3445550/150‑1 (MQ=60)
                                                                                                TTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCT                                                   <  SRR19973066.3445744/150‑1 (MQ=60)
                                                                                                TTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCT                                                   <  SRR19973066.2126163/150‑1 (MQ=60)
                                                                                                   TGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGC                                                <  SRR19973066.2361891/150‑1 (MQ=60)
                                                                                                   TGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGC                                                >  SRR19973066.113546/1‑150 (MQ=60)
                                                                                                       GAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCG                                            <  SRR19973066.1114372/150‑1 (MQ=60)
                                                                                                       GAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCG                                            >  SRR19973066.1330545/1‑150 (MQ=60)
                                                                                                        AGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGA                                           >  SRR19973066.1333338/1‑150 (MQ=60)
                                                                                                        AGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGA                                           >  SRR19973066.1333323/1‑150 (MQ=60)
                                                                                                        AGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGA                                           <  SRR19973066.2820556/150‑1 (MQ=60)
                                                                                                        AGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGA                                           >  SRR19973066.3118863/1‑150 (MQ=60)
                                                                                                           ATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATC                                        <  SRR19973066.3015582/150‑1 (MQ=60)
                                                                                                           ATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATC                                        >  SRR19973066.2379115/1‑150 (MQ=60)
                                                                                                           ATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATC                                        >  SRR19973066.2378789/1‑150 (MQ=60)
                                                                                                                   ACCTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAA                                >  SRR19973066.1479018/1‑150 (MQ=60)
                                                                                                                     CTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGT                              >  SRR19973066.405928/1‑150 (MQ=60)
                                                                                                                     CTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGT                              <  SRR19973066.810798/150‑1 (MQ=60)
                                                                                                                     CTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGT                              <  SRR19973066.690571/150‑1 (MQ=60)
                                                                                                                     CTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGT                              <  SRR19973066.690218/150‑1 (MQ=60)
                                                                                                                     CTCGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGT                              >  SRR19973066.406118/1‑150 (MQ=60)
                                                                                                                       CGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGG                            >  SRR19973066.457696/1‑150 (MQ=60)
                                                                                                                       CGCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGG                            <  SRR19973066.2783178/150‑1 (MQ=60)
                                                                                                                        GCTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGA                           <  SRR19973066.2981130/150‑1 (MQ=60)
                                                                                                                         CTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAT                          >  SRR19973066.170469/1‑150 (MQ=60)
                                                                                                                         CTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAT                          >  SRR19973066.170492/1‑150 (MQ=60)
                                                                                                                         CTGGAAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGAT                          >  SRR19973066.804446/1‑150 (MQ=60)
                                                                                                                             AAGCGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGAGGAAGTGGATGCCG                      >  SRR19973066.2264026/1‑150 (MQ=60)
                                                                                                                                CGATGGCGGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCA                   >  SRR19973066.2530990/1‑150 (MQ=60)
                                                                                                                                       GGAAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTG            >  SRR19973066.1038603/1‑150 (MQ=60)
                                                                                                                                         AAAGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTG          <  SRR19973066.649428/150‑1 (MQ=60)
                                                                                                                                           AGCGATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCG        >  SRR19973066.1708860/1‑150 (MQ=60)
                                                                                                                                              GATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGG     >  SRR19973066.1380043/1‑150 (MQ=60)
                                                                                                                                              GATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGG     >  SRR19973066.1210590/1‑150 (MQ=60)
                                                                                                                                              GATGGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGG     >  SRR19973066.2999474/1‑150 (MQ=60)
                                                                                                                                                 GGCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAA  <  SRR19973066.3424052/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                    
CAGTTCGTCGAGGCCGCCCATGAGAGCGGTAAATACAAGTTAACCGCCGTATATTCCCGCAGCCTTGAACAGGCCCAGCACTTCGCCAA‑TGATTTTTCTGTCGAGCATCTGTTTACCTCGCTGGAAGCGATGGCGGAAAGCGATGCCATTGACGCGGTGTATATTGCCAGCCCGAATTCCCTGCATTTTTCCCAGACACAACTTTTCCTTAGCCATAAAATTAATGTGATTTGCGAGAAACCGCTGGCGTCGAATCTGGCGGAAGTGGATGCCGCCATTGCCTGTGCGCGGGAA  >  CP009273/3230712‑3231005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑