Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,028,224 G→T 28.7% L104I (CTC→ATC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,028,2240GT28.7% 611.4 / 233.4 295L104I (CTC→ATC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (101/109);  new base T (35/50);  total (136/159)
Fisher's exact test for biased strand distribution p-value = 3.04e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

CAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCG  >  CP009273/3028075‑3028372
                                                                                                                                                     |                                                                                                                                                    
   cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATc                                                                                                                                                   <  2:1978338/150‑1 (MQ=255)
   cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGACGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATc                                                                                                                                                   <  2:2910102/150‑1 (MQ=255)
       cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCa                                                                                                                                               <  2:3252516/150‑1 (MQ=255)
                    tCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACa                                                                                                                                  >  2:1294174/1‑150 (MQ=255)
                     cagcagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAg                                                                                                                                 <  2:4606392/150‑1 (MQ=255)
                        cagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTg                                                                                                                              >  2:3056035/1‑150 (MQ=255)
                        cagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTg                                                                                                                              >  1:2669241/1‑150 (MQ=255)
                              cAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCg                                                                                                                        <  1:4577136/150‑1 (MQ=255)
                               aaCTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa                                                                                                                       >  1:3931501/1‑150 (MQ=255)
                                    ggTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGcc                                                                                                                  <  2:3180164/150‑1 (MQ=255)
                                      tGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCaa                                                                                                                >  2:1754264/1‑150 (MQ=255)
                                      tGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCaa                                                                                                                >  1:274729/1‑150 (MQ=255)
                                        cAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg                                                                                                              <  2:3545956/150‑1 (MQ=255)
                                        cAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg                                                                                                              >  1:4709202/1‑150 (MQ=255)
                                           tGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTcc                                                                                                           >  1:3556315/1‑150 (MQ=255)
                                            ggATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCt                                                                                                          <  1:359619/150‑1 (MQ=255)
                                             gATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg                                                                                                         <  2:4316669/150‑1 (MQ=255)
                                              aTAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGc                                                                                                        <  1:2295880/150‑1 (MQ=255)
                                                aGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAg                                                                                                      <  1:3956607/150‑1 (MQ=255)
                                                aGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAg                                                                                                      >  1:347903/1‑150 (MQ=255)
                                                 gACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg                                                                                                     <  2:8572/150‑1 (MQ=255)
                                                  aCAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCATCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg                                                                                                    >  1:418781/1‑150 (MQ=255)
                                                    aGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTa                                                                                                  >  2:2537351/1‑150 (MQ=255)
                                                    aGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTa                                                                                                  <  2:1398526/150‑1 (MQ=255)
                                                       tCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACg                                                                                               >  1:2663771/1‑150 (MQ=255)
                                                       tCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACg                                                                                               >  1:1577553/1‑150 (MQ=255)
                                                       tCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACg                                                                                               <  1:4552139/150‑1 (MQ=255)
                                                         gCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa                                                                                             >  1:3727405/1‑150 (MQ=255)
                                                         gCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaa                                                                                             >  1:497507/1‑150 (MQ=255)
                                                          ccGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa                                                                                            >  2:2601874/1‑150 (MQ=255)
                                                          ccGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa                                                                                            >  1:3037071/1‑150 (MQ=255)
                                                             gttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc                                                                                         >  1:3314918/1‑150 (MQ=255)
                                                              ttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg                                                                                        <  1:2783770/150‑1 (MQ=255)
                                                              ttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg                                                                                        <  2:2446528/150‑1 (MQ=255)
                                                                 tggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCtt                                                                                     >  1:203293/1‑150 (MQ=255)
                                                                   gttttgAACAGCTTACCTTTCGCGCCGAGGATGTCTCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa                                                                                   >  2:2696869/1‑150 (MQ=255)
                                                                   gttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa                                                                                   >  2:2101844/1‑150 (MQ=255)
                                                                    ttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAc                                                                                  <  1:3346093/150‑1 (MQ=255)
                                                                        gAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat                                                                              <  1:3776679/150‑1 (MQ=255)
                                                                        gAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat                                                                              >  2:3990309/1‑150 (MQ=255)
                                                                         aaCAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTCACGAAAGACGCTTTACCCata                                                                             >  2:4344402/1‑150 (MQ=255)
                                                                          aCAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa                                                                            <  2:4101471/150‑1 (MQ=255)
                                                                           cAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat                                                                           <  1:1659148/150‑1 (MQ=255)
                                                                              cTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg                                                                        >  1:608740/1‑150 (MQ=255)
                                                                                  ccTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg                                                                    >  1:3899668/1‑150 (MQ=255)
                                                                                    tttCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt                                                                  <  1:460029/150‑1 (MQ=255)
                                                                                      tCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca                                                                <  1:2585054/150‑1 (MQ=255)
                                                                                       cgcgCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat                                                               <  1:1706336/150‑1 (MQ=255)
                                                                                        gcgcCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc                                                              <  2:3694367/150‑1 (MQ=255)
                                                                                        gcgcCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc                                                              <  2:3794168/150‑1 (MQ=255)
                                                                                          gcCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat                                                            >  2:1071142/1‑150 (MQ=255)
                                                                                           ccGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCTACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTATCGAAAGACGCTTTACCCATAATACGACGGGTCATCATg                                                           <  1:1858289/150‑1 (MQ=255)
                                                                                            cGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc                                                          >  2:1512393/1‑150 (MQ=255)
                                                                                            cGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc                                                          <  1:1085817/150‑1 (MQ=255)
                                                                                             gAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTCACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCg                                                         >  1:2839672/1‑150 (MQ=255)
                                                                                                 aTGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa                                                     <  1:1189345/150‑1 (MQ=255)
                                                                                                 aTGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCa                                                     <  2:3404686/150‑1 (MQ=255)
                                                                                                   gTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc                                                   >  1:694765/1‑150 (MQ=255)
                                                                                                     cGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa                                                 >  2:1126630/1‑150 (MQ=255)
                                                                                                      gCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAc                                                <  2:570715/150‑1 (MQ=255)
                                                                                                       ccGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg                                               <  1:4140119/150‑1 (MQ=255)
                                                                                                       ccGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACg                                               <  1:4517875/150‑1 (MQ=255)
                                                                                                         gAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc                                             >  1:4119884/1‑150 (MQ=255)
                                                                                                         gAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc                                             >  1:2852842/1‑150 (MQ=255)
                                                                                                          aGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCg                                            >  2:2716119/1‑150 (MQ=255)
                                                                                                            gTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGAc                                          >  2:3346745/1‑150 (MQ=255)
                                                                                                             tCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACt                                         >  1:3025182/1‑150 (MQ=255)
                                                                                                              cccATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACtt                                        >  2:2862622/1‑150 (MQ=255)
                                                                                                                cATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCg                                      <  1:1257233/150‑1 (MQ=255)
                                                                                                                 aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGa                                     <  2:3926662/150‑1 (MQ=255)
                                                                                                                  ttttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt                                    <  2:539879/150‑1 (MQ=255)
                                                                                                                  ttttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGAt                                    <  1:1296922/150‑1 (MQ=255)
                                                                                                                   tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg                                   >  1:4612993/1‑150 (MQ=255)
                                                                                                                   tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg                                   <  2:4725239/150‑1 (MQ=255)
                                                                                                                   tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg                                   >  1:3658207/1‑150 (MQ=255)
                                                                                                                    ttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGt                                  >  1:2410629/1‑150 (MQ=255)
                                                                                                                      ttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTc                                >  1:1379391/1‑150 (MQ=255)
                                                                                                                           cTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcgc                           >  1:3880843/1‑150 (MQ=255)
                                                                                                                            tGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt                          >  2:2784879/1‑150 (MQ=255)
                                                                                                                              cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                        <  1:2200204/150‑1 (MQ=255)
                                                                                                                                    ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttc                  <  1:85325/150‑1 (MQ=255)
                                                                                                                                    ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttc                  >  2:4017585/1‑150 (MQ=255)
                                                                                                                                    ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttc                  >  2:2283523/1‑150 (MQ=255)
                                                                                                                                     taAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct                 <  1:2536945/150‑1 (MQ=255)
                                                                                                                                     taAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct                 <  2:3456804/150‑1 (MQ=255)
                                                                                                                                      aaaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt                >  1:2217762/1‑150 (MQ=255)
                                                                                                                                       aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc               <  1:145990/150‑1 (MQ=255)
                                                                                                                                       aaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc               <  1:2139491/150‑1 (MQ=255)
                                                                                                                                        aCGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg              <  2:418781/150‑1 (MQ=255)
                                                                                                                                          gCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt            <  2:1155964/150‑1 (MQ=255)
                                                                                                                                          gCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt            <  1:4031027/150‑1 (MQ=255)
                                                                                                                                           ccTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtc           <  2:640625/150‑1 (MQ=255)
                                                                                                                                            cTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct          >  2:2332225/1‑150 (MQ=255)
                                                                                                                                             ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc         <  2:4463957/150‑1 (MQ=255)
                                                                                                                                             ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc         <  2:2253413/150‑1 (MQ=255)
                                                                                                                                              tCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct        <  1:2163821/150‑1 (MQ=255)
                                                                                                                                               ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt       >  1:1946221/1‑150 (MQ=255)
                                                                                                                                                cGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt      <  2:2968084/150‑1 (MQ=255)
                                                                                                                                                 gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg     >  1:3234744/1‑150 (MQ=255)
                                                                                                                                                    agaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCg  >  2:4517739/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCG  >  CP009273/3028075‑3028372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A