Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,028,224 G→T 5.4% L104I (CTC→ATC)  lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,028,2240GT 5.4% 866.1 / 18.0 220L104I (CTC→ATC) lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (106/102);  new base T (3/9);  total (109/111)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 228 total aligned reads displayed.

CAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGT  >  CP009273/3028075‑3028373
                                                                                                                                                     |                                                                                                                                                     
 aGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGgaga                                                                                                                                                      >  1:22446/1‑150 (MQ=255)
 aGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGgaga                                                                                                                                                      >  1:1796209/1‑150 (MQ=255)
  gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGAt                                                                                                                                                     <  1:496488/150‑1 (MQ=255)
    ggACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCg                                                                                                                                                   <  1:1138682/150‑1 (MQ=255)
       cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCa                                                                                                                                                <  2:1120640/150‑1 (MQ=255)
       cGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATATTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCa                                                                                                                                                >  2:2136915/1‑150 (MQ=255)
            tGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGc                                                                                                                                           <  1:2412818/150‑1 (MQ=255)
             gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCa                                                                                                                                          >  2:5766/1‑150 (MQ=255)
               tttGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAAc                                                                                                                                        <  1:2315597/150‑1 (MQ=255)
                ttGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACg                                                                                                                                       >  1:3186918/1‑150 (MQ=255)
                  ggTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTa                                                                                                                                     <  2:1554415/150‑1 (MQ=255)
                         agACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGa                                                                                                                              <  1:1571014/150‑1 (MQ=255)
                          gACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGaa                                                                                                                             <  1:1825893/150‑1 (MQ=255)
                            cGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATg                                                                                                                           <  1:1609691/150‑1 (MQ=255)
                            cGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATg                                                                                                                           >  1:2117435/1‑150 (MQ=255)
                             gCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGc                                                                                                                          <  1:3993081/150‑1 (MQ=255)
                              cAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCg                                                                                                                         >  1:679862/1‑150 (MQ=255)
                              cAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCg                                                                                                                         >  2:806588/1‑150 (MQ=255)
                               aaCTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGa                                                                                                                        <  1:147884/150‑1 (MQ=255)
                                aCTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACg                                                                                                                                       <  2:2651591/134‑1 (MQ=255)
                                aCTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACg                                                                                                                                       >  1:2651592/1‑134 (MQ=255)
                                 cTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGAcc                                                                                                                      <  1:3579537/150‑1 (MQ=255)
                                 cTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGAcc                                                                                                                      >  2:2419478/1‑150 (MQ=255)
                                  tCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGCACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg                                                                                                                     <  2:3721971/150‑1 (MQ=255)
                                    ggTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGcc                                                                                                                   >  1:441557/1‑150 (MQ=255)
                                     gTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCa                                                                                                                  >  2:3991836/1‑150 (MQ=255)
                                         aGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGt                                                                                                              >  2:2052562/1‑150 (MQ=255)
                                          gTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc                                                                                                             >  1:659565/1‑150 (MQ=255)
                                              aTAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc                                                                                                         >  2:141538/1‑150 (MQ=255)
                                                aGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAg                                                                                                       >  2:1251721/1‑150 (MQ=255)
                                                   cAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt                                                                                                    <  1:2425229/150‑1 (MQ=255)
                                                     gTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa                                                                                                  <  2:2731752/150‑1 (MQ=255)
                                                      ttCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAAc                                                                                                 <  1:3329226/150‑1 (MQ=255)
                                                       tCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACg                                                                                                <  1:1035246/150‑1 (MQ=255)
                                                        cGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa                                                                                               >  1:2741149/1‑150 (MQ=255)
                                                          ccGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa                                                                                             >  1:3919037/1‑150 (MQ=255)
                                                            ggttttggttttGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGa                                                                                           >  1:1447716/1‑150 (MQ=255)
                                                            ggttttggttttGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGa                                                                                           >  1:1683937/1‑150 (MQ=255)
                                                             gttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc                                                                                          <  2:1681124/150‑1 (MQ=255)
                                                             gttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGAc                                                                                          <  2:2837927/150‑1 (MQ=255)
                                                              ttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg                                                                                         <  1:1680290/150‑1 (MQ=255)
                                                              ttttggttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTACTGCAGGGTAACGAAAGACg                                                                                         >  2:1359965/1‑150 (MQ=255)
                                                                   gttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa                                                                                    <  1:3146348/150‑1 (MQ=255)
                                                                    ttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAc                                                                                   >  1:311147/1‑150 (MQ=255)
                                                                    ttttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAc                                                                                   >  2:4100594/1‑150 (MQ=255)
                                                                      ttgAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTAccc                                                                                 >  1:252849/1‑150 (MQ=255)
                                                                        gAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat                                                                               >  1:1705017/1‑150 (MQ=255)
                                                                        gAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat                                                                               <  2:1636854/150‑1 (MQ=255)
                                                                          aCAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa                                                                             >  1:3547226/1‑150 (MQ=255)
                                                                          aCAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa                                                                             >  1:228283/1‑150 (MQ=255)
                                                                            aGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataata                                                                           >  1:2742966/1‑150 (MQ=255)
                                                                              cTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg                                                                         >  1:2030959/1‑150 (MQ=255)
                                                                                tACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac                                                                       <  2:854562/150‑1 (MQ=255)
                                                                                  ccTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg                                                                     >  1:1175166/1‑150 (MQ=255)
                                                                                  ccTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg                                                                     >  2:3807438/1‑150 (MQ=255)
                                                                                     ttCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtc                                                                  >  1:609035/1‑150 (MQ=255)
                                                                                       cgcgCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat                                                                >  2:1962965/1‑150 (MQ=255)
                                                                                         cgcCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca                                                              >  2:3082079/1‑150 (MQ=255)
                                                                                            cGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc                                                           <  1:1884372/150‑1 (MQ=255)
                                                                                            cGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGc                                                           >  1:3922965/1‑150 (MQ=255)
                                                                                             gAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCg                                                          >  1:2828168/1‑150 (MQ=255)
                                                                                                   gTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGc                                                    >  1:1857181/1‑150 (MQ=255)
                                                                                                     cGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa                                                  <  1:2431153/150‑1 (MQ=255)
                                                                                                      gCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAAc                                                 >  2:440391/1‑150 (MQ=255)
                                                                                                        cGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg                                               <  2:605973/150‑1 (MQ=255)
                                                                                                        cGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACgg                                               >  2:3219615/1‑150 (MQ=255)
                                                                                                         gAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc                                              >  2:2315991/1‑150 (MQ=255)
                                                                                                         gAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGc                                              >  2:657935/1‑150 (MQ=255)
                                                                                                           ggTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCTTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGa                                            <  2:1818250/150‑1 (MQ=255)
                                                                                                           ggTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGa                                            >  2:3032889/1‑150 (MQ=255)
                                                                                                             tCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACt                                          <  1:4135531/150‑1 (MQ=255)
                                                                                                               ccATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc                                        <  1:1958854/150‑1 (MQ=255)
                                                                                                               ccATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTc                                        >  1:3662485/1‑150 (MQ=255)
                                                                                                                   tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATg                                    <  1:3649197/150‑1 (MQ=255)
                                                                                                                    ttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGt                                   <  1:1376234/150‑1 (MQ=255)
                                                                                                                     tttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGtt                                  <  2:3813805/150‑1 (MQ=255)
                                                                                                                         aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgc                              >  1:831496/1‑150 (MQ=255)
                                                                                                                             gCTCGTTATAAACGCCTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCtt                          <  2:148474/150‑1 (MQ=255)
                                                                                                                              cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                         >  2:2784584/1‑150 (MQ=255)
                                                                                                                              cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc                         <  1:1381039/150‑1 (MQ=255)
                                                                                                                                   tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGtt                    <  1:298137/150‑1 (MQ=255)
                                                                                                                                     taAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct                  >  1:2784730/1‑150 (MQ=255)
                                                                                                                                     taAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct                  <  2:3817011/150‑1 (MQ=255)
                                                                                                                                     taAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttct                  <  1:1710337/150‑1 (MQ=255)
                                                                                                                                      aaaCGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt                 <  1:1106800/150‑1 (MQ=255)
                                                                                                                                         cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGt              >  1:1013726/1‑150 (MQ=255)
                                                                                                                                         cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGt              <  1:3535337/150‑1 (MQ=255)
                                                                                                                                          gCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt             <  2:704661/150‑1 (MQ=255)
                                                                                                                                           ccTTCCGGGATATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtc            >  2:2608229/1‑150 (MQ=255)
                                                                                                                                           ccTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtc            >  2:3114506/1‑150 (MQ=255)
                                                                                                                                           ccTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtc            <  2:1957997/150‑1 (MQ=255)
                                                                                                                                              tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct         <  2:1893169/150‑1 (MQ=255)
                                                                                                                                              tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct         >  1:3696336/1‑150 (MQ=255)
                                                                                                                                              tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct         >  2:2476442/1‑150 (MQ=255)
                                                                                                                                               ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt        <  2:3587008/150‑1 (MQ=255)
                                                                                                                                                cGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt       <  2:1612533/150‑1 (MQ=255)
                                                                                                                                                 gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg      >  1:3613906/1‑150 (MQ=255)
                                                                                                                                                 gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg      >  2:456049/1‑150 (MQ=255)
                                                                                                                                                    agaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCg   >  2:3368825/1‑150 (MQ=255)
                                                                                                                                                     gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGt  <  1:3268338/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGTCCCATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGT  >  CP009273/3028075‑3028373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A