Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F159 I0 R1 879 305.9 9588012 96.6% 9262019 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,504,143 G→T 47.7% R65S (CGT→AGT)  yhfX ← putative pyridoxal 5'‑phosphate binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,504,1430GT47.7% 151.7 / inf 306R65S (CGT→AGT) yhfXputative pyridoxal 5'‑phosphate binding protein
Reads supporting (aligned to +/- strand):  ref base G (68/92);  new base T (58/88);  total (126/180)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 307 total aligned reads displayed.

AGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCG  >  CP009273/3503994‑3504291
                                                                                                                                                     |                                                                                                                                                    
   aaCCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTAcc                                                                                                                                                   <  1:3694112/150‑1 (MQ=255)
    aCCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCa                                                                                                                                                  >  2:351421/1‑150 (MQ=255)
      cTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCTGCCAGCCACGGATTACGACCaaa                                                                                                                                                <  2:1155879/150‑1 (MQ=255)
      cTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCaaa                                                                                                                                                >  1:3635827/1‑150 (MQ=255)
         atgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTg                                                                                                                                             >  1:2579603/1‑150 (MQ=255)
         atgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTg                                                                                                                                             <  2:2555647/150‑1 (MQ=255)
         atgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTg                                                                                                                                             <  2:435982/150‑1 (MQ=255)
          tgaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGc                                                                                                                                            >  1:1029597/1‑150 (MQ=255)
           gaCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCt                                                                                                                                           <  2:2907727/150‑1 (MQ=255)
            aCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCCCAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCtt                                                                                                                                          >  1:1423948/1‑150 (MQ=255)
            aCAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCtt                                                                                                                                          >  2:382721/1‑150 (MQ=255)
             cAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTg                                                                                                                                         <  2:2988723/150‑1 (MQ=255)
               aGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGt                                                                                                                                       >  1:1489116/1‑150 (MQ=255)
               aGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGt                                                                                                                                       <  2:1330651/150‑1 (MQ=255)
               aGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGt                                                                                                                                       >  1:1817366/1‑150 (MQ=255)
                gggATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtc                                                                                                                                      <  2:1251954/150‑1 (MQ=255)
                 ggATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCCGCCACGGATTACTACCAAACTGCTGGGtca                                                                                                                                     >  2:2460835/1‑150 (MQ=255)
                  gATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGtcat                                                                                                                                    <  1:2385785/150‑1 (MQ=255)
                   aTTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGtcatc                                                                                                                                   <  1:381274/150‑1 (MQ=255)
                    tttGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGtcatca                                                                                                                                  >  2:4627860/1‑150 (MQ=255)
                       gTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGAt                                                                                                                               <  1:193895/150‑1 (MQ=255)
                       gTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGAt                                                                                                                               <  2:1386549/150‑1 (MQ=255)
                        tACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATa                                                                                                                              <  2:4014314/150‑1 (MQ=255)
                        tACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATa                                                                                                                              >  1:1870109/1‑150 (MQ=255)
                            aGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGt                                                                                                                          >  2:3294463/1‑150 (MQ=255)
                            aGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGt                                                                                                                          >  2:3038012/1‑150 (MQ=255)
                              aTGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTc                                                                                                                        <  1:3228142/150‑1 (MQ=255)
                              aTGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTc                                                                                                                        <  1:4502494/150‑1 (MQ=255)
                               tGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCa                                                                                                                       >  1:2109737/1‑150 (MQ=255)
                                  cccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATg                                                                                                                    <  1:1432805/150‑1 (MQ=255)
                                   ccTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGc                                                                                                                   >  2:4305913/1‑150 (MQ=255)
                                    cTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGcc                                                                                                                  <  2:3356912/150‑1 (MQ=255)
                                      gATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGt                                                                                                                <  2:869273/150‑1 (MQ=255)
                                        tGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCTGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTaa                                                                                                              <  1:3275812/150‑1 (MQ=255)
                                          cgcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAg                                                                                                            <  2:2825988/150‑1 (MQ=255)
                                          cgcCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAg                                                                                                            <  2:875848/150‑1 (MQ=255)
                                            ccACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGcc                                                                                                          <  1:3430304/150‑1 (MQ=255)
                                             cacaGGCAAAGCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCcg                                                                                                         <  2:703288/150‑1 (MQ=255)
                                                aGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCgcgc                                                                                                      >  2:2542735/1‑150 (MQ=255)
                                                     aaCCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGtct                                                                                                 <  2:1073705/150‑1 (MQ=255)
                                                        cAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCaa                                                                                              >  2:2838655/1‑150 (MQ=255)
                                                         aGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAAt                                                                                             <  2:154346/150‑1 (MQ=255)
                                                           cgcgGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCa                                                                                           <  2:1887210/150‑1 (MQ=255)
                                                              ggcgCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGcc                                                                                        <  1:2639885/150‑1 (MQ=255)
                                                                 gcATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCt                                                                                     <  2:3025143/150‑1 (MQ=255)
                                                                  cATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCtt                                                                                    <  2:1699710/150‑1 (MQ=255)
                                                                   aTGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTa                                                                                   <  2:4740245/150‑1 (MQ=255)
                                                                     gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAcc                                                                                 <  1:3560669/150‑1 (MQ=255)
                                                                     gACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTAcc                                                                                 <  1:4540503/150‑1 (MQ=255)
                                                                      aCTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCg                                                                                >  1:252922/1‑150 (MQ=255)
                                                                       cTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGt                                                                               >  2:3573628/1‑150 (MQ=255)
                                                                         cgcgCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGttt                                                                             <  2:3915815/150‑1 (MQ=255)
                                                                         cgcgCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGttt                                                                             <  1:564754/150‑1 (MQ=255)
                                                                         cgcgCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGttt                                                                             <  2:231622/150‑1 (MQ=255)
                                                                            gcCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTcc                                                                          >  1:4016171/1‑150 (MQ=255)
                                                                             ccTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCa                                                                         >  2:733046/1‑150 (MQ=255)
                                                                              ctctTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAg                                                                        <  2:4099374/150‑1 (MQ=255)
                                                                              ctctTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAg                                                                        >  2:367829/1‑150 (MQ=255)
                                                                              ctctTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAg                                                                        <  2:4530656/150‑1 (MQ=255)
                                                                                     tAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGa                                                                 >  2:592978/1‑150 (MQ=255)
                                                                                         ccACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCa                                                             <  1:1720470/150‑1 (MQ=255)
                                                                                            ccGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGt                                                          <  1:2815553/150‑1 (MQ=255)
                                                                                             cGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTc                                                         <  1:1058384/150‑1 (MQ=255)
                                                                                              gCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCg                                                        >  2:3415444/1‑150 (MQ=255)
                                                                                              gCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCg                                                        <  1:3350884/150‑1 (MQ=255)
                                                                                              gCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCg                                                        <  1:3891974/150‑1 (MQ=255)
                                                                                               ccACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGa                                                       <  1:2309020/150‑1 (MQ=255)
                                                                                               ccACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGa                                                       >  1:2742582/1‑150 (MQ=255)
                                                                                                cacaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGAt                                                      <  2:448654/150‑1 (MQ=255)
                                                                                                cacaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGAt                                                      <  1:2523343/150‑1 (MQ=255)
                                                                                                 acaATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATc                                                     <  2:2109737/150‑1 (MQ=255)
                                                                                                     tGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGAATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCAccc                                                 >  2:3110057/1‑150 (MQ=255)
                                                                                                         gCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCt                                             >  1:3824920/1‑150 (MQ=255)
                                                                                                           tGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGt                                           <  1:731650/150‑1 (MQ=255)
                                                                                                             tAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTcc                                         <  1:3087127/150‑1 (MQ=255)
                                                                                                                ccTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCggg                                      >  2:2018883/1‑150 (MQ=255)
                                                                                                                 cTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCgggg                                     >  1:120724/1‑150 (MQ=255)
                                                                                                                 cTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCgggg                                     <  2:1817366/150‑1 (MQ=255)
                                                                                                                   aaTGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCg                                   >  2:4781961/1‑150 (MQ=255)
                                                                                                                   aaTGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCg                                   >  1:3874342/1‑150 (MQ=255)
                                                                                                                     tGCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGAt                                 >  2:2382602/1‑150 (MQ=255)
                                                                                                                      gCCAGCACTTTGTCCGCCAGCCACGGATTACGACCAACCTGCTTGGTCATCAGATACAGGTCACTGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATc                                <  2:3635827/150‑1 (MQ=255)
                                                                                                                      gCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATc                                <  2:4564498/150‑1 (MQ=255)
                                                                                                                      gCCAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATc                                <  1:4170239/150‑1 (MQ=255)
                                                                                                                       ccAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCt                               >  1:4656404/1‑150 (MQ=255)
                                                                                                                        cAGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCtt                              >  1:1587823/1‑150 (MQ=255)
                                                                                                                         aGCAATTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTg                             >  1:2259633/1‑150 (MQ=255)
                                                                                                                            aaTTTTTCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGccc                          <  2:4183490/150‑1 (MQ=255)
                                                                                                                            aaTTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGCTCTTGccc                          >  1:439158/1‑150 (MQ=255)
                                                                                                                            aaTTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGccc                          >  1:2450269/1‑150 (MQ=255)
                                                                                                                              tttttCCGCCAGCCACGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCctg                        <  2:161071/150‑1 (MQ=255)
                                                                                                                                      ccagccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCcaca                <  1:2489174/150‑1 (MQ=255)
                                                                                                                                          ccaCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGGAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCt            <  2:1655886/150‑1 (MQ=255)
                                                                                                                                           caCGGATTACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCtt           <  2:3233599/150‑1 (MQ=255)
                                                                                                                                            aCGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTa          <  2:1870109/150‑1 (MQ=255)
                                                                                                                                              ggATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGt        <  1:4645790/150‑1 (MQ=255)
                                                                                                                                              ggATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGt        <  2:370896/150‑1 (MQ=255)
                                                                                                                                                 ttACTACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcg     >  1:4281321/1‑150 (MQ=255)
                                                                                                                                                   aCGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcggc   <  2:4132074/150‑1 (MQ=255)
                                                                                                                                                    cGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTgcggcg  <  2:43302/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
AGCAACCTGATGACAAGGGATTTGTACCAGATGCCCCTGATGCGCCACAGGCAAACCAGCGCGGCGCATGACTCGCGCCTCTTTGTAATCCACCGCCACAATGCCGCTGTAGCCTAATGCCAGCAATTTTTCCGCCAGCCACGGATTACGACCAAACTGCTTGGTCATCAGATACAGTTCAATGCCGTAAAGCCGCGCCGTCTCAATCAGCCGCTTACCGTTTTCCAGTATCTGATCCACGTCGATCACCCAGCTGTCCGGGGCGATCTTGCCCTGCTGCCACAGGCTTAGTGCGGCG  >  CP009273/3503994‑3504291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A