Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,713,571 G→T 17.1% H97N (CAC→AAC)  anmK ← anhydro‑N‑acetylmuramic acid kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,713,5710GT17.1% 586.4 / 71.3 198H97N (CAC→AAC) anmKanhydro‑N‑acetylmuramic acid kinase
Reads supporting (aligned to +/- strand):  ref base G (74/90);  new base T (9/25);  total (83/115)
Fisher's exact test for biased strand distribution p-value = 5.60e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 198 total aligned reads displayed.

TGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTA  >  CP009273/1713425‑1713717
                                                                                                                                                  |                                                                                                                                                  
tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGa                                                                                                                                                   <  2:2349531/148‑1 (MQ=255)
tGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGa                                                                                                                                                   <  1:332222/148‑1 (MQ=255)
  cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACa                                                                                                                                                 <  1:2670923/148‑1 (MQ=255)
  cgcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACa                                                                                                                                                 <  1:3239596/148‑1 (MQ=255)
   gcCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACaa                                                                                                                                                <  2:1589502/148‑1 (MQ=255)
      ttGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCg                                                                                                                                             <  1:1033630/148‑1 (MQ=255)
       tGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGa                                                                                                                                            <  2:1752822/148‑1 (MQ=255)
        gTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                                                                                                                           <  2:2913478/148‑1 (MQ=255)
        gTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                                                                                                                           <  2:3477128/148‑1 (MQ=255)
        gTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGAt                                                                                                                                           <  2:3477067/148‑1 (MQ=255)
          cctccCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCg                                                                                                                                         >  2:487455/1‑148 (MQ=255)
             cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                                                                                                                      <  2:2715112/148‑1 (MQ=255)
             cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                                                                                                                      >  2:3156624/1‑148 (MQ=255)
             cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                                                                                                                      >  2:186672/1‑148 (MQ=255)
             cccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCaa                                                                                                                                      <  2:3332248/148‑1 (MQ=255)
              ccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAGTCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAAc                                                                                                                                     <  2:3166027/148‑1 (MQ=255)
              ccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAAc                                                                                                                                     >  1:2256172/1‑148 (MQ=255)
              ccAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAAc                                                                                                                                     <  1:3387889/148‑1 (MQ=255)
               cAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACt                                                                                                                                    <  2:3753475/148‑1 (MQ=255)
               cAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACt                                                                                                                                    >  1:2749620/1‑148 (MQ=255)
                 aGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACtat                                                                                                                                  <  1:695196/148‑1 (MQ=255)
                 aGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACtat                                                                                                                                  <  2:3104838/148‑1 (MQ=255)
                  ggCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACtata                                                                                                                                 >  1:2015192/1‑148 (MQ=255)
                   gCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTatat                                                                                                                                <  1:2048389/148‑1 (MQ=255)
                     aaTATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATAtct                                                                                                                              <  2:2015192/148‑1 (MQ=255)
                     aaTATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATAtct                                                                                                                              <  1:3340515/148‑1 (MQ=255)
                     aaTATCGCGACGGCGAAAATCACCGACAACCGTAATTCCAGTGCGTGCCACAAATTGATTGTTATCGCCAATCAGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATAtct                                                                                                                              >  1:3219097/1‑148 (MQ=255)
                      atatCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATAtctc                                                                                                                             <  1:1362722/148‑1 (MQ=255)
                         tCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTg                                                                                                                          <  2:1517791/148‑1 (MQ=255)
                           gcgACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACTATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCTTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGcc                                                                                                                        <  1:1915342/148‑1 (MQ=255)
                             gACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTg                                                                                                                      <  2:1729748/148‑1 (MQ=255)
                                     aaaTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt                                                                                                              >  1:3191182/1‑148 (MQ=255)
                                     aaaTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGAtttt                                                                                                              >  1:3427429/1‑148 (MQ=255)
                                      aaTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg                                                                                                             >  1:2965189/1‑148 (MQ=255)
                                      aaTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTg                                                                                                             <  2:1975985/148‑1 (MQ=255)
                                       aTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGt                                                                                                            >  2:1320770/1‑148 (MQ=255)
                                       aTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGt                                                                                                            >  1:317222/1‑148 (MQ=255)
                                       aTCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGt                                                                                                            >  1:2241725/1‑148 (MQ=255)
                                          aCCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTcc                                                                                                         <  1:99935/148‑1 (MQ=255)
                                          aCCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTcc                                                                                                         >  1:3631879/1‑148 (MQ=255)
                                              aCAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTaa                                                                                                     >  1:1729245/1‑148 (MQ=255)
                                               cAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAg                                                                                                    <  2:970984/148‑1 (MQ=255)
                                                 aCCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCa                                                                                                  <  1:1469011/148‑1 (MQ=255)
                                                  ccGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCaa                                                                                                 <  2:490807/148‑1 (MQ=255)
                                                  ccGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCaa                                                                                                 >  2:1200805/1‑148 (MQ=255)
                                                   cGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAg                                                                                                <  2:2552149/148‑1 (MQ=255)
                                                    gTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAgg                                                                                               <  2:2041184/148‑1 (MQ=255)
                                                    gTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAgg                                                                                               <  2:1121000/148‑1 (MQ=255)
                                                     tAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGc                                                                                              >  2:821707/1‑148 (MQ=255)
                                                        ttCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCAtt                                                                                           >  2:1803750/1‑148 (MQ=255)
                                                         tCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTg                                                                                          <  2:1249359/148‑1 (MQ=255)
                                                         tCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTg                                                                                          <  2:1018962/148‑1 (MQ=255)
                                                         tCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTg                                                                                          <  1:3256134/148‑1 (MQ=255)
                                                             gtgcgtgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCg                                                                                      >  2:1975007/1‑148 (MQ=255)
                                                               gcgtgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCa                                                                                    <  1:3633421/148‑1 (MQ=255)
                                                                  tgcCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCa                                                                                 <  2:3038190/148‑1 (MQ=255)
                                                                    ccACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGc                                                                               >  1:2330590/1‑148 (MQ=255)
                                                                        aaTTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCaaaa                                                                           <  2:2103479/148‑1 (MQ=255)
                                                                        aaTTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCaaaa                                                                           <  2:863904/148‑1 (MQ=255)
                                                                         aTTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCaaaaa                                                                          <  1:1328610/148‑1 (MQ=255)
                                                                          tttGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAg                                                                         <  1:3037550/148‑1 (MQ=255)
                                                                          tttGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAg                                                                         >  2:1080115/1‑148 (MQ=255)
                                                                          tttGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAg                                                                         >  1:2624315/1‑148 (MQ=255)
                                                                          tttGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAg                                                                         <  2:2255694/148‑1 (MQ=255)
                                                                           ttGATTGTTATCGCCACTCTGTACAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATCTCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGt                                                                        <  2:2660789/148‑1 (MQ=255)
                                                                           ttGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGt                                                                        <  1:487455/148‑1 (MQ=255)
                                                                                      cGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTg                                                             >  1:1398308/1‑148 (MQ=255)
                                                                                        ccAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAg                                                           <  2:770615/148‑1 (MQ=255)
                                                                                        ccAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAg                                                           <  2:770150/148‑1 (MQ=255)
                                                                                         cAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGt                                                          <  1:2000027/148‑1 (MQ=255)
                                                                                           aTCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTAt                                                        >  1:1788818/1‑148 (MQ=255)
                                                                                             cTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCa                                                      >  1:3771034/1‑148 (MQ=255)
                                                                                              tGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCaa                                                     <  1:742582/148‑1 (MQ=255)
                                                                                               gTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAg                                                    >  1:1900993/1‑148 (MQ=255)
                                                                                                tAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGc                                                   <  2:2330590/148‑1 (MQ=255)
                                                                                                  aaGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTg                                                 >  1:3118647/1‑148 (MQ=255)
                                                                                                  aaGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTg                                                 >  1:3117638/1‑148 (MQ=255)
                                                                                                    gtgtgtGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTc                                               <  2:3219097/148‑1 (MQ=255)
                                                                                                      gtgtgGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCa                                             >  2:2398400/1‑148 (MQ=255)
                                                                                                        gtggtgCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCaaa                                           <  2:3191822/148‑1 (MQ=255)
                                                                                                        gtggtgCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCaaa                                           <  2:1568411/148‑1 (MQ=255)
                                                                                                        gtggtgCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCaaa                                           <  2:394238/148‑1 (MQ=255)
                                                                                                              ccACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGa                                     >  2:448709/1‑148 (MQ=255)
                                                                                                                aCGCCCGTCGGTTCATGCCAGACGGTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGAATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGaaa                                   <  1:3555756/148‑1 (MQ=255)
                                                                                                                   cccGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAgtg                                <  2:791797/148‑1 (MQ=255)
                                                                                                                     cGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTa                              <  1:813617/148‑1 (MQ=255)
                                                                                                                       tCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAg                            >  2:2149279/1‑148 (MQ=255)
                                                                                                                       tCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAg                            >  2:2996071/1‑148 (MQ=255)
                                                                                                                         ggTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAgct                          <  2:3427429/148‑1 (MQ=255)
                                                                                                                           ttCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAgctgc                        <  1:3686030/148‑1 (MQ=255)
                                                                                                                            tCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAgctgct                       >  1:3793873/1‑148 (MQ=255)
                                                                                                                                gCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGcc                   <  1:3653496/148‑1 (MQ=255)
                                                                                                                                gCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGcc                   <  1:3653425/148‑1 (MQ=255)
                                                                                                                                     aCGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCCGCAAAAAGTTGCCCGAGGTGAGTAGGCCGCTGTCCAATGTGCGCCAGGGGGCGCTgctggcgctggc              >  2:3612594/1‑148 (MQ=255)
                                                                                                                                       ggTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCaa            <  1:215034/148‑1 (MQ=255)
                                                                                                                                       ggTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCaa            <  1:515463/148‑1 (MQ=255)
                                                                                                                                        gTTTGACCGTTACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCaaa           >  2:629965/1‑148 (MQ=255)
                                                                                                                                                gTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGt   <  1:1436974/148‑1 (MQ=255)
                                                                                                                                                gTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGt   <  1:1436507/148‑1 (MQ=255)
                                                                                                                                                 tGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTa  >  1:1549315/1‑148 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
TGCGCCTTGTCCTCCCAAGGCAATATCGCGACGGCGAAAATCACCGACAACCGTAATTCCGGTGCGTGCCACAATTTGATTGTTATCGCCAATCTGTAAAGTGTGTGGTGCCACGCCCGTCGGTTCATGCCAGACGGTTTGACCGTGACAACCGATCGCAACTATATCTCTTGCCTGCAGATTTTGTTCCTTAAGCAAGGCATTGACCGCATCAGCAAAAAGTTGCCCGAGTTGAGTATCAAGCTGTCCAAACTGCGAAAGTGTAAGCTGCTGGCCCTGGCAAATATCCAGTA  >  CP009273/1713425‑1713717

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A