Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,801,706 C→A 100% pseudogene (32/1178 nt) ygaY → pseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,801,7060CA98.6% 720.9 / 0.5 220pseudogene (32/1178 nt)ygaYpseudogene, major facilitator transporter superfamily;putative transport; Not classified; putative transport protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (131/86);  total (132/88)
Fisher's exact test for biased strand distribution p-value = 5.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 220 total aligned reads displayed.

ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGC  >  CP009273/2801563‑2801848
                                                                                                                                               |                                                                                                                                              
aTCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTg                                                                                                                                            <  1:3474892/148‑1 (MQ=255)
 tCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGa                                                                                                                                           <  1:1015135/148‑1 (MQ=255)
 tCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGa                                                                                                                                           >  1:645259/1‑148 (MQ=255)
  cATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAt                                                                                                                                          >  2:168221/1‑148 (MQ=255)
  cATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAt                                                                                                                                          >  1:1564885/1‑148 (MQ=255)
   aTGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATc                                                                                                                                         >  1:81529/1‑148 (MQ=255)
       cTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGc                                                                                                                                     <  1:928679/148‑1 (MQ=255)
        tACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCt                                                                                                                                    >  1:1623047/1‑148 (MQ=255)
         aCAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTTTTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTg                                                                                                                                   <  2:2207959/148‑1 (MQ=255)
               cacaTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCt                                                                                                                             >  1:1482523/1‑148 (MQ=255)
                acctCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTa                                                                                                                            <  2:1399222/145‑1 (MQ=255)
                acaTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTa                                                                                                                            <  1:2129818/148‑1 (MQ=255)
                 caTCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAt                                                                                                                           >  1:2887281/1‑148 (MQ=255)
                    cGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGc                                                                                                                        >  1:1956900/1‑148 (MQ=255)
                        caTTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCAcc                                                                                                                    >  1:2110089/1‑148 (MQ=255)
                           ttCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGt                                                                                                                 >  1:3766610/1‑148 (MQ=255)
                                  atctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCgg                                                                                                          >  1:115309/1‑148 (MQ=255)
                                   tctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGt                                                                                                         >  1:1356482/1‑148 (MQ=255)
                                    ctatctTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCggga                                                                                                        >  2:2875124/1‑146 (MQ=255)
                                        ctTTCATCCCATATTCATCAACATCCGCTCTTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCGTATCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCa                                                                                                    >  2:2035675/1‑148 (MQ=255)
                                         tttCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAg                                                                                                   >  2:2225002/1‑148 (MQ=255)
                                            cATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTACACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTaa                                                                                                <  2:1443121/148‑1 (MQ=255)
                                               cccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTa                                                                                             >  1:783118/1‑148 (MQ=255)
                                               cccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTa                                                                                             >  2:1795676/1‑148 (MQ=255)
                                                ccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTAt                                                                                            <  2:1976858/148‑1 (MQ=255)
                                                ccATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTAt                                                                                            >  1:2007741/1‑148 (MQ=255)
                                                  atatTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTa                                                                                          <  1:2847243/148‑1 (MQ=255)
                                                     ttCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGc                                                                                       >  2:1225269/1‑148 (MQ=255)
                                                       catcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGccc                                                                                     <  1:704623/148‑1 (MQ=255)
                                                       catcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGccc                                                                                     <  1:3420061/148‑1 (MQ=255)
                                                        atcaACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCa                                                                                    >  2:1045441/1‑148 (MQ=255)
                                                            aCATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCa                                                                                >  2:2245755/1‑148 (MQ=255)
                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  2:70887/148‑1 (MQ=255)
                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               >  1:2197632/1‑148 (MQ=255)
                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  2:70246/148‑1 (MQ=255)
                                                             cATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAt                                                                               <  1:3771093/148‑1 (MQ=255)
                                                               tCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTg                                                                             >  1:3585170/1‑148 (MQ=255)
                                                                ccGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGc                                                                            <  1:2127686/148‑1 (MQ=255)
                                                                 cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCt                                                                           >  2:2802607/1‑148 (MQ=255)
                                                                 cGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCt                                                                           <  1:2515470/148‑1 (MQ=255)
                                                                   cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                         >  1:2931830/1‑148 (MQ=255)
                                                                   cTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCg                                                                         >  2:487332/1‑148 (MQ=255)
                                                                    tattatTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGa                                                                        <  2:863260/148‑1 (MQ=255)
                                                                    tagtattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGa                                                                        <  2:2776393/145‑1 (MQ=255)
                                                                       tattGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGATGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGacac                                                                     >  1:2987741/1‑148 (MQ=255)
                                                                         ttGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCa                                                                   <  1:2578510/148‑1 (MQ=255)
                                                                                 cAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGt                                                           <  2:754748/148‑1 (MQ=255)
                                                                                 cAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGt                                                           >  2:1296647/1‑148 (MQ=255)
                                                                                  aGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTCTTACGCCCAGCCATTGCTCGACACCATCGCGCGTa                                                          >  2:1706526/1‑148 (MQ=255)
                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         >  2:1969236/1‑148 (MQ=255)
                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         <  2:308349/148‑1 (MQ=255)
                                                                                   gCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTaa                                                         <  2:3353356/148‑1 (MQ=255)
                                                                                     ttAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGAAACTATAACGCCCAGCCATTGATCGACACCATCGCGCGAAACt                                                       >  1:1490920/1‑148 (MQ=255)
                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTAGACACCATCGCGCGTAACtt                                                      >  2:358132/1‑148 (MQ=255)
                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  1:2172446/148‑1 (MQ=255)
                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      >  2:366708/1‑148 (MQ=255)
                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  1:244449/148‑1 (MQ=255)
                                                                                      tAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACtt                                                      <  2:2264107/148‑1 (MQ=255)
                                                                                         tcatcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTc                                                   <  2:2784987/148‑1 (MQ=255)
                                                                                          catcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTcc                                                  >  1:1758386/1‑148 (MQ=255)
                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 >  1:1838682/1‑148 (MQ=255)
                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 <  1:122266/148‑1 (MQ=255)
                                                                                           atcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTccc                                                 <  2:1844543/148‑1 (MQ=255)
                                                                                            tcaCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCt                                                <  2:2043499/148‑1 (MQ=255)
                                                                                             caCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCtt                                               >  1:492369/1‑148 (MQ=255)
                                                                                              aCCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCttt                                              <  1:1054252/148‑1 (MQ=255)
                                                                                               ccTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTc                                             <  2:2914311/148‑1 (MQ=255)
                                                                                                 tgatgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCg                                           >  1:1655844/1‑148 (MQ=255)
                                                                                                   atgaACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTAGACACCATCGCGCGTAACTTTTCCCTTTACGcc                                         <  1:2563135/148‑1 (MQ=255)
                                                                                                      aaCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGt                                      <  2:642996/148‑1 (MQ=255)
                                                                                                      aaCAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGt                                      <  2:2887158/148‑1 (MQ=255)
                                                                                                        cAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGACAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTc                                    >  2:2512705/1‑148 (MQ=255)
                                                                                                           aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                 <  2:948889/148‑1 (MQ=255)
                                                                                                           aaaTAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGc                                 >  1:741770/1‑148 (MQ=255)
                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  2:844015/1‑148 (MQ=255)
                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  1:2912598/1‑148 (MQ=255)
                                                                                                            aataatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCa                                >  1:876504/1‑148 (MQ=255)
                                                                                                              taatGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAgg                              >  2:1358210/1‑148 (MQ=255)
                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:2057071/1‑148 (MQ=255)
                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:1151634/1‑148 (MQ=255)
                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            >  2:1077012/1‑148 (MQ=255)
                                                                                                                atGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCt                            <  1:3220897/148‑1 (MQ=255)
                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  2:3035414/148‑1 (MQ=255)
                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         >  1:2419949/1‑148 (MQ=255)
                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  1:3264011/148‑1 (MQ=255)
                                                                                                                   aCTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTa                         <  1:3263997/148‑1 (MQ=255)
                                                                                                                    cTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAt                        >  2:2047644/1‑148 (MQ=255)
                                                                                                                    cTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAt                        >  1:2135241/1‑148 (MQ=255)
                                                                                                                      aaaCCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTg                      <  1:635527/148‑1 (MQ=255)
                                                                                                                       aaCCTACTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGt                     <  1:3387989/148‑1 (MQ=255)
                                                                                                                          cTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAc                  <  2:401011/148‑1 (MQ=255)
                                                                                                                          cTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAc                  <  2:853668/148‑1 (MQ=255)
                                                                                                                            aaTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAccg                >  2:2318116/1‑148 (MQ=255)
                                                                                                                              tCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTAccgcc              >  1:332212/1‑148 (MQ=255)
                                                                                                                                 tGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCcgcg           >  2:362221/1‑148 (MQ=255)
                                                                                                                                 tGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCcgcg           >  1:3345510/1‑148 (MQ=255)
                                                                                                                                     cTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGt       >  2:1549470/1‑148 (MQ=255)
                                                                                                                                       tAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTg     >  2:1193648/1‑148 (MQ=255)
                                                                                                                                         gCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTggg   <  2:2529225/148‑1 (MQ=255)
                                                                                                                                          cccGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGc  <  2:3610371/148‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                              
ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGC  >  CP009273/2801563‑2801848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTATGCCG  >  CP009273/2801560‑2801855
                                                                                                                                                  |                                                                                                                                                     
CTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCT                                                                                                                                                    <  SRR19973069.88043/150‑1 (MQ=60)
CTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCT                                                                                                                                                    <  SRR19973069.1214595/150‑1 (MQ=60)
  TATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGA                                                                                                                                                  >  SRR19973069.649500/1‑150 (MQ=60)
   ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAT                                                                                                                                                 <  SRR19973069.3497354/150‑1 (MQ=60)
   ATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGAT                                                                                                                                                 >  SRR19973069.1575559/1‑150 (MQ=60)
    TCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATC                                                                                                                                                <  SRR19973069.1022136/150‑1 (MQ=60)
    TCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATC                                                                                                                                                >  SRR19973069.82413/1‑150 (MQ=60)
    TCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATC                                                                                                                                                <  SRR19973069.934566/150‑1 (MQ=60)
         CCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCT                                                                                                                                           >  SRR19973069.2546008/1‑150 (MQ=60)
         CCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCT                                                                                                                                           >  SRR19973069.1634068/1‑150 (MQ=60)
          CTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTG                                                                                                                                          <  SRR19973069.934986/150‑1 (MQ=60)
                ACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCT                                                                                                                                    >  SRR19973069.1492833/1‑150 (MQ=60)
                ACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCT                                                                                                                                    >  SRR19973069.1493004/1‑150 (MQ=60)
                 CCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTA                                                                                                                                   >  SRR19973069.1192797/1‑150 (MQ=60)
                  CACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAT                                                                                                                                  >  SRR19973069.2906173/1‑150 (MQ=60)
                  CACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTAT                                                                                                                                  >  SRR19973069.2906296/1‑150 (MQ=60)
                   ACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATC                                                                                                                                 <  SRR19973069.2144466/150‑1 (MQ=60)
                   ACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATC                                                                                                                                 <  SRR19973069.2144323/150‑1 (MQ=60)
                   ACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATC                                                                                                                                 <  SRR19973069.2144507/150‑1 (MQ=60)
                    CATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCG                                                                                                                                <  SRR19973069.1556286/150‑1 (MQ=60)
                    CATCGCACATTTCAACAATCAATCTTTAATCCCCTATAAATCAACAACCGCTAATATTGATTTCCAGCTAAATCATCACCTGATGAACAAAAATAAAGACAAAACCTAAACATTAGATAAGCCCCGACCTGATCGTGCGGATGTCTATCG                                                                                                                                >  SRR19973069.159768/1‑150 (MQ=53)
                     ATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGC                                                                                                                               >  SRR19973069.1970456/1‑150 (MQ=60)
                        GCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCAC                                                                                                                            >  SRR19973069.1024485/1‑150 (MQ=60)
                         CACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACC                                                                                                                           >  SRR19973069.2124643/1‑150 (MQ=60)
                            ATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGT                                                                                                                        >  SRR19973069.3791090/1‑150 (MQ=60)
                            ATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGT                                                                                                                        >  SRR19973069.3791678/1‑150 (MQ=60)
                                CAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGG                                                                                                                    >  SRR19973069.647209/1‑150 (MQ=60)
                                   CAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGG                                                                                                                 >  SRR19973069.116436/1‑150 (MQ=60)
                                    AATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGT                                                                                                                >  SRR19973069.1365905/1‑150 (MQ=60)
                                        TATCTTTCATCCCATATTCATCAACATCCGCGATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCC                                                                                                            >  SRR19973069.2847988/1‑150 (MQ=60)
                                           CTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGT                                                                                                         >  SRR19973069.1642258/1‑150 (MQ=60)
                                                ATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTA                                                                                                    >  SRR19973069.346282/1‑150 (MQ=60)
                                                ATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTA                                                                                                    >  SRR19973069.1985226/1‑150 (MQ=60)
                                                ATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTA                                                                                                    >  SRR19973069.788671/1‑150 (MQ=60)
                                                ATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTA                                                                                                    >  SRR19973069.788719/1‑150 (MQ=60)
                                                 TCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTAT                                                                                                   >  SRR19973069.2021712/1‑150 (MQ=60)
                                                    CATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTAC                                                                                                <  SRR19973069.3352082/150‑1 (MQ=60)
                                                    CATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTAC                                                                                                >  SRR19973069.2444064/1‑150 (MQ=60)
                                                     ATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACG                                                                                               <  SRR19973069.2866006/150‑1 (MQ=60)
                                                     ATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACG                                                                                               >  SRR19973069.1389705/1‑150 (MQ=60)
                                                       ATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCC                                                                                             >  SRR19973069.3752937/1‑150 (MQ=60)
                                                         TCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCA                                                                                           <  SRR19973069.2176199/150‑1 (MQ=60)
                                                         TCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCA                                                                                           >  SRR19973069.1463972/1‑150 (MQ=60)
                                                          CATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAG                                                                                          <  SRR19973069.709576/150‑1 (MQ=60)
                                                          CATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAG                                                                                          <  SRR19973069.709568/150‑1 (MQ=60)
                                                          CATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAG                                                                                          <  SRR19973069.2926894/150‑1 (MQ=60)
                                                          CATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAG                                                                                          <  SRR19973069.3442323/150‑1 (MQ=60)
                                                           ATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGC                                                                                         >  SRR19973069.3014623/1‑150 (MQ=60)
                                                              AACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCAT                                                                                      >  SRR19973069.2212560/1‑150 (MQ=60)
                                                                CCTCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTG                                                                                    <  SRR19973069.3053946/150‑1 (MQ=60)
                                                                CATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTG                                                                                    <  SRR19973069.3795370/150‑1 (MQ=60)
                                                                CATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTG                                                                                    >  SRR19973069.3608553/1‑150 (MQ=60)
                                                                CATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTG                                                                                    <  SRR19973069.3795588/150‑1 (MQ=60)
                                                                CATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTG                                                                                    >  SRR19973069.2399896/1‑150 (MQ=60)
                                                                   CCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTC                                                                                 <  SRR19973069.2142323/150‑1 (MQ=60)
                                                                    CGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCG                                                                                >  SRR19973069.2951259/1‑150 (MQ=60)
                                                                    CGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCG                                                                                <  SRR19973069.2532214/150‑1 (MQ=60)
                                                                    CGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCG                                                                                >  SRR19973069.459561/1‑150 (MQ=60)
                                                                      CTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGAC                                                                              >  SRR19973069.500041/1‑150 (MQ=60)
                                                                        ATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACAC                                                                            >  SRR19973069.2778812/1‑150 (MQ=60)
                                                                        ATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACAC                                                                            >  SRR19973069.953014/1‑150 (MQ=60)
                                                                        ATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGATGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACAC                                                                            >  SRR19973069.3007587/1‑150 (MQ=60)
                                                                            TTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATC                                                                        <  SRR19973069.2595635/150‑1 (MQ=60)
                                                                            TTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATC                                                                        <  SRR19973069.2595488/150‑1 (MQ=60)
                                                                                  TCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGT                                                                  <  SRR19973069.3409617/150‑1 (MQ=60)
                                                                                  TCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGT                                                                  <  SRR19973069.744146/150‑1 (MQ=60)
                                                                                      GCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACT                                                              <  SRR19973069.1953009/150‑1 (MQ=60)
                                                                                      GCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGAAACTATAACGCCCAGCCATTGATCGACACCATCGCGCGAAACT                                                              >  SRR19973069.1501274/1‑150 (MQ=60)
                                                                                        TTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTT                                                            <  SRR19973069.2130777/150‑1 (MQ=60)
                                                                                         TAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTT                                                           >  SRR19973069.943696/1‑150 (MQ=60)
                                                                                         TAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTT                                                           <  SRR19973069.246268/150‑1 (MQ=60)
                                                                                         TAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTT                                                           <  SRR19973069.2187276/150‑1 (MQ=60)
                                                                                           ATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCC                                                         >  SRR19973069.3259375/1‑150 (MQ=60)
                                                                                           ATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCC                                                         >  SRR19973069.1770777/1‑150 (MQ=60)
                                                                                            TCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCC                                                        >  SRR19973069.1851548/1‑150 (MQ=60)
                                                                                              ATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTT                                                      <  SRR19973069.123545/150‑1 (MQ=60)
                                                                                              ATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTT                                                      <  SRR19973069.123427/150‑1 (MQ=60)
                                                                                              ATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTT                                                      >  SRR19973069.495912/1‑150 (MQ=60)
                                                                                                 ACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCC                                                   <  SRR19973069.1061576/150‑1 (MQ=60)
                                                                                                  CCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCG                                                  >  SRR19973069.1667304/1‑150 (MQ=60)
                                                                                                    TGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATTGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCC                                                >  SRR19973069.1724395/1‑150 (MQ=60)
                                                                                                     GATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCA                                               <  SRR19973069.1837788/150‑1 (MQ=60)
                                                                                                      ATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTAGACACCATCGCGCGTAACTTTTCCCTTTACGCCAG                                              <  SRR19973069.2580062/150‑1 (MQ=60)
                                                                                                      ATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAG                                              >  SRR19973069.1676677/1‑150 (MQ=60)
                                                                                                            AAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGC                                        >  SRR19973069.747024/1‑150 (MQ=60)
                                                                                                            AAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGC                                        >  SRR19973069.2199357/1‑150 (MQ=60)
                                                                                                             AAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCA                                       >  SRR19973069.2932344/1‑150 (MQ=60)
                                                                                                             AAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCA                                       >  SRR19973069.882560/1‑150 (MQ=60)
                                                                                                             AAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCA                                       >  SRR19973069.882593/1‑150 (MQ=60)
                                                                                                             AAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCA                                       >  SRR19973069.2931864/1‑150 (MQ=60)
                                                                                                              AAATAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAG                                      >  SRR19973069.3564337/1‑150 (MQ=60)
                                                                                                                 TAATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCT                                   <  SRR19973069.247702/150‑1 (MQ=60)
                                                                                                                   ATGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTT                                 <  SRR19973069.3241936/150‑1 (MQ=60)
                                                                                                                    TGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTA                                >  SRR19973069.2436294/1‑150 (MQ=60)
                                                                                                                    TGACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTA                                >  SRR19973069.2436266/1‑150 (MQ=60)
                                                                                                                     GACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTAT                               >  SRR19973069.2149916/1‑150 (MQ=60)
                                                                                                                      ACTAAACCTCATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATT                              <  SRR19973069.3285389/150‑1 (MQ=60)
                                                                                                                      ACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATT                              <  SRR19973069.3285393/150‑1 (MQ=60)
                                                                                                                      ACTAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATT                              <  SRR19973069.3285379/150‑1 (MQ=60)
                                                                                                                        TAAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGT                            >  SRR19973069.2134727/1‑150 (MQ=60)
                                                                                                                         AAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTT                           >  SRR19973069.110217/1‑150 (MQ=60)
                                                                                                                         AAACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTT                           <  SRR19973069.639713/150‑1 (MQ=60)
                                                                                                                          AACCTACTCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTA                          <  SRR19973069.3410121/150‑1 (MQ=60)
                                                                                                                          AACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTA                          <  SRR19973069.3409432/150‑1 (MQ=60)
                                                                                                                          AACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTA                          <  SRR19973069.3410214/150‑1 (MQ=60)
                                                                                                                          AACCTAATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTA                          >  SRR19973069.2057626/1‑150 (MQ=60)
                                                                                                                               AATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCC                     >  SRR19973069.729506/1‑150 (MQ=60)
                                                                                                                               AATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCC                     >  SRR19973069.334499/1‑150 (MQ=60)
                                                                                                                               AATCATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCC                     >  SRR19973069.333786/1‑150 (MQ=60)
                                                                                                                                  CATGAGCTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCG                  >  SRR19973069.3367408/1‑150 (MQ=60)
                                                                                                                                        CTTAGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTG            <  SRR19973069.2993172/150‑1 (MQ=60)
                                                                                                                                           AGCCCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGC         >  SRR19973069.779936/1‑150 (MQ=60)
                                                                                                                                              CCGGAGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTAT      >  SRR19973069.2104822/1‑150 (MQ=60)
                                                                                                                                                  AGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTATGCCG  <  SRR19973069.983274/150‑1 (MQ=60)
                                                                                                                                                  AGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCGTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTATGCCG  <  SRR19973069.983220/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                     
CTTATCATGCCTACAAACCACATCGCACATTTCAACAATCTATCTTTCATCCCATATTCATCAACATCCGCTATTATTGATTTCCAGCTTAATCATCACCTGATGAACAAAAATAATGACTAAACCTAATCATGAGCTTAGCCCGGCGCTGATCGTGCTGATGTCTATCGCCACCGGTCTGGCGGTAGCCAGTAACTATTACGCCCAGCCATTGCTCGACACCATCGCGCGTAACTTTTCCCTTTCCGCCAGTTCGGCAGGCTTTATTGTTACCGCCGCGCAGTTGGGCTATGCCG  >  CP009273/2801560‑2801855

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑