Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,545,077 C→A 20.4% L252L (CTG→CTT)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,545,0770CA20.4% 534.1 / 88.5 200L252L (CTG→CTT) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (88/71);  new base A (20/21);  total (108/92)
Fisher's exact test for biased strand distribution p-value = 4.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01

Only 100 of 202 total aligned reads displayed.

GTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCC  >  CP009273/3544930‑3545224
                                                                                                                                                   |                                                                                                                                                   
gTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTc                                                                                                                                                     <  1:2052438/148‑1 (MQ=255)
    tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  1:2272996/1‑148 (MQ=255)
    tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  1:931404/1‑148 (MQ=255)
    tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  2:1869585/1‑148 (MQ=255)
    tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtt                                                                                                                                                 >  1:2091520/1‑148 (MQ=255)
       gCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGttttt                                                                                                                                              <  1:2440169/148‑1 (MQ=255)
        ccAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTc                                                                                                                                             <  2:3638901/148‑1 (MQ=255)
            ttCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCa                                                                                                                                         >  1:202609/1‑148 (MQ=255)
             tCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCAt                                                                                                                                        <  1:2275790/148‑1 (MQ=255)
             tCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCAt                                                                                                                                        >  2:628098/1‑148 (MQ=255)
               gTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTg                                                                                                                                      <  1:1864469/148‑1 (MQ=255)
               gTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTg                                                                                                                                      <  1:323545/148‑1 (MQ=255)
                tGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGa                                                                                                                                     <  1:2544952/148‑1 (MQ=255)
                 gaaGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATGAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGag                                                                                                                                    <  2:2205491/146‑2 (MQ=255)
                 gCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGAt                                                                                                                                    <  2:2204801/148‑1 (MQ=255)
                 gCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGAt                                                                                                                                    >  2:294416/1‑148 (MQ=255)
                   aGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGc                                                                                                                                  <  1:3575083/148‑1 (MQ=255)
                     tttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGccc                                                                                                                                >  2:826357/1‑148 (MQ=255)
                     tttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGccc                                                                                                                                >  1:1452367/1‑148 (MQ=255)
                     tttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGccc                                                                                                                                >  1:736610/1‑148 (MQ=255)
                      ttACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCct                                                                                                                               >  1:655921/1‑148 (MQ=255)
                        aCGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctgc                                                                                                                             <  2:3163634/148‑1 (MQ=255)
                          gCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctgctg                                                                                                                           <  2:1024458/148‑1 (MQ=255)
                              cGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCg                                                                                                                       >  1:971051/1‑148 (MQ=255)
                                ccAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGc                                                                                                                     >  2:2115401/1‑148 (MQ=255)
                                    atgatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                 >  1:3656754/1‑148 (MQ=255)
                                     tgatgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCa                                                                                                                <  2:1784761/148‑1 (MQ=255)
                                       atgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAg                                                                                                              >  2:644628/1‑148 (MQ=255)
                                       atgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAg                                                                                                              >  2:2565332/1‑148 (MQ=255)
                                        tgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGa                                                                                                             >  1:2168311/1‑148 (MQ=255)
                                         ggCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGag                                                                                                            >  1:3771669/1‑147 (MQ=255)
                                           cGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAAt                                                                                                          >  2:413350/1‑148 (MQ=255)
                                             aCGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCa                                                                                                        <  2:1167313/148‑1 (MQ=255)
                                             aCGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCa                                                                                                        <  2:1166510/148‑1 (MQ=255)
                                                   aaTATCCGCTACCGAACAGGCACACTTGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  >  1:1385545/1‑148 (MQ=255)
                                                   aaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  <  1:3271372/148‑1 (MQ=255)
                                                   aaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                  <  2:1790135/148‑1 (MQ=255)
                                                      atCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTa                                                                                               >  1:3772538/1‑148 (MQ=255)
                                                       tCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaa                                                                                              <  1:1804018/148‑1 (MQ=255)
                                                        ccGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaaa                                                                                             <  2:3541569/148‑1 (MQ=255)
                                                          gCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAtt                                                                                           >  2:2510617/1‑148 (MQ=255)
                                                           cTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAttt                                                                                          <  2:1103916/148‑1 (MQ=255)
                                                             aCCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAg                                                                                        <  1:1279734/148‑1 (MQ=255)
                                                               cGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTc                                                                                      >  2:1238278/1‑148 (MQ=255)
                                                               cGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTATTc                                                                                      >  2:2128507/1‑148 (MQ=255)
                                                                  aCAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGa                                                                                   <  2:3665241/148‑1 (MQ=255)
                                                                  aCAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTACGCATTGATGCCCAGCTGTTCGGCACGCAACAAATCACCGTCGGTTAATATAGACAGa                                                                                   >  1:149372/1‑148 (MQ=255)
                                                                   cAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGAt                                                                                  >  2:3371216/1‑148 (MQ=255)
                                                                       cacaCTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCaaa                                                                              <  1:451532/148‑1 (MQ=255)
                                                                         cacTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATAAAAcc                                                                            >  1:865342/1‑147 (MQ=255)
                                                                         cacTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACg                                                                            >  1:3725869/1‑148 (MQ=255)
                                                                          acTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACgg                                                                           <  1:3348189/148‑1 (MQ=255)
                                                                           cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGa                                                                          <  1:2869565/148‑1 (MQ=255)
                                                                           cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGa                                                                          <  1:2883481/148‑1 (MQ=255)
                                                                           cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGa                                                                          <  1:3663782/148‑1 (MQ=255)
                                                                           cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGGTCGGCACGCAAGAAATCACCGGCGGTTCATGTGGTCAGATCCCACggg                                                                          >  1:3424997/1‑147 (MQ=255)
                                                                           cTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGa                                                                          <  2:588151/148‑1 (MQ=255)
                                                                             ggAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATAAAAAGGATg                                                                        >  1:1854426/1‑148 (MQ=255)
                                                                                    ttGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCgcgtgc                                                                 >  2:1443959/1‑148 (MQ=255)
                                                                                       ctgcATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTc                                                              >  2:3292749/1‑148 (MQ=255)
                                                                                          cATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGcc                                                           <  1:1860498/148‑1 (MQ=255)
                                                                                            tCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCCGATCAAAAGGATGCGCGTGCGGCGccgg                                                         >  1:1862812/1‑146 (MQ=255)
                                                                                                gcgcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCcac                                                     <  2:1497689/148‑1 (MQ=255)
                                                                                                gcgcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCcac                                                     <  2:2800367/148‑1 (MQ=255)
                                                                                                   cAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATAAAAAGGATGCGCGTGCGTCGCCAGCcacaca                                                  >  1:1556167/1‑146 (MQ=255)
                                                                                                   cAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGa                                                  <  1:754824/148‑1 (MQ=255)
                                                                                                     gCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCCCAGACg                                                >  2:3746088/1‑148 (MQ=255)
                                                                                                      cTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTGTATTTTTTGAGATCAAAACGATTGCCGTGCGTCGCCTGCCACAGACGc                                               >  1:762376/1‑148 (MQ=255)
                                                                                                            aCCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGc                                         <  1:596995/148‑1 (MQ=255)
                                                                                                             ccGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCAAAGACGCAGCGGCt                                        >  2:3436964/1‑148 (MQ=255)
                                                                                                             ccGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAAAGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCt                                        >  2:465221/1‑148 (MQ=255)
                                                                                                                   gTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgcca                                  >  2:555925/1‑148 (MQ=255)
                                                                                                                     aTGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTTAAATTATTCAGATAAAAAGGATTCGCGTGCGTAGCCAGCCACACACGCCACGGCGGcgccccc                                >  1:1754582/1‑145 (MQ=255)
                                                                                                                     aTGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAAAGGATGCGCGTGCGTCGCCAGCCACAGACGCAGCCGCAGcgccacc                                >  2:1768118/1‑147 (MQ=255)
                                                                                                                        gTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacgcca                             <  1:2978924/148‑1 (MQ=255)
                                                                                                                        gTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacgcca                             <  1:2978732/148‑1 (MQ=255)
                                                                                                                           gTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTa                          <  1:3469773/148‑1 (MQ=255)
                                                                                                                             cGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACg                        <  2:3090672/148‑1 (MQ=255)
                                                                                                                                ttGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACCCACCCAGCGGCTGCGCCCCGCCATTACGATa                     >  1:2163385/1‑148 (MQ=255)
                                                                                                                                ttGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATa                     >  2:3779539/1‑148 (MQ=255)
                                                                                                                                  ggATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGc                   <  1:913807/148‑1 (MQ=255)
                                                                                                                                  ggATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGc                   <  1:292925/148‑1 (MQ=255)
                                                                                                                                   gATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCAGCCACAGACCCAGCGGCTGCGCCACCCCATTACGATAGcc                  >  1:2109268/1‑148 (MQ=255)
                                                                                                                                   gATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAAAGGATGCGCGTGCGTCGCCAGCCACACACGCAGCCGCTGCGCCACGCCAATACCATAGcc                  >  1:1176644/1‑148 (MQ=255)
                                                                                                                                     tAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGa                >  2:1964188/1‑148 (MQ=255)
                                                                                                                                      agagAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCCCGCCATTACGATAGCCGAc               >  2:3804306/1‑148 (MQ=255)
                                                                                                                                      agagAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCCTTACGATATCCGAc               >  2:1697685/1‑148 (MQ=255)
                                                                                                                                        agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             <  2:2899550/148‑1 (MQ=255)
                                                                                                                                        agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             <  1:644628/148‑1 (MQ=255)
                                                                                                                                        agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             <  2:1484218/148‑1 (MQ=255)
                                                                                                                                        agaACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACaa             >  2:953232/1‑148 (MQ=255)
                                                                                                                                            cTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGAAAAAAAGGCTGCGCGTGCGTCGCCTGCCCCAGAAGCAGCGGCGGCGCCCCCCCATTAAGATAGACGACAACggg         >  1:498051/1‑148 (MQ=255)
                                                                                                                                            cTTTCGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTAAGAACAAAAGTATGAGCGAGAGTCCGCAGCCACAAACCCACCCGCATACCCCCGCCAATACCAGAGCCGCCAAcctg         >  1:397523/1‑145 (MQ=255)
                                                                                                                                             tttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATACGACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACgggg        >  2:895313/1‑148 (MQ=255)
                                                                                                                                              ttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTGAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATATCCGACAACCGGga       >  1:305206/1‑148 (MQ=255)
                                                                                                                                              ttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGga       <  2:1385545/148‑1 (MQ=255)
                                                                                                                                                ggTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGgaga     <  1:2366912/148‑1 (MQ=255)
                                                                                                                                                 gTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGAt    <  2:1489010/148‑1 (MQ=255)
                                                                                                                                                  tCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATc   <  1:2678842/148‑1 (MQ=255)
                                                                                                                                                   cAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCCCAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATcc  >  1:270464/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
GTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCC  >  CP009273/3544930‑3545224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A