Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,545,077 C→A 29.1% L252L (CTG→CTT)  malP ← maltodextrin phosphorylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,545,0770CA29.1% 581.9 / 198.2 288L252L (CTG→CTT) malPmaltodextrin phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (113/91);  new base A (45/39);  total (158/130)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 290 total aligned reads displayed.

TCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCC  >  CP009273/3544928‑3545225
                                                                                                                                                     |                                                                                                                                                    
      tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtttt                                                                                                                                                <  2:2367546/150‑1 (MQ=255)
      tCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGtttt                                                                                                                                                <  1:444166/150‑1 (MQ=255)
        cGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTc                                                                                                                                              <  2:75313/150‑1 (MQ=255)
         gCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTcc                                                                                                                                             <  1:3245905/150‑1 (MQ=255)
          ccAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCg                                                                                                                                            <  1:3013881/150‑1 (MQ=255)
           cAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGc                                                                                                                                           >  2:4434043/1‑150 (MQ=255)
             gTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCAt                                                                                                                                         >  2:855556/1‑150 (MQ=255)
               tCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTg                                                                                                                                       <  2:4055457/150‑1 (MQ=255)
                 gTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGAt                                                                                                                                     <  2:1825769/150‑1 (MQ=255)
                 gTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGAt                                                                                                                                     >  1:2093046/1‑150 (MQ=255)
                 gTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGAt                                                                                                                                     <  1:4757822/150‑1 (MQ=255)
                  tGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATg                                                                                                                                    >  1:3577305/1‑150 (MQ=255)
                      gTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCct                                                                                                                                >  2:2977657/1‑150 (MQ=255)
                       tttACGCCCCGACAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACATTGGTAAGTTTTTCCGCATTGATGCCctg                                                                                                                               >  1:2732844/1‑150 (MQ=255)
                         tACGCCCCGCCAGATGATGGAGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGTATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCctgct                                                                                                                             >  2:375810/1‑150 (MQ=255)
                            gCCCCGCCTGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGTACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGtt                                                                                                                          <  1:539448/150‑1 (MQ=255)
                             ccccGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTc                                                                                                                         <  1:1722064/150‑1 (MQ=255)
                             ccccGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTc                                                                                                                         <  2:1154833/150‑1 (MQ=255)
                              cccGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTACGCATTGATGCCCTGCTGTTCg                                                                                                                        >  1:4606460/1‑150 (MQ=255)
                                   cAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACg                                                                                                                   <  2:1675282/150‑1 (MQ=255)
                                    aGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                  >  2:274880/1‑150 (MQ=255)
                                    aGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGc                                                                                                                  >  2:4932511/1‑150 (MQ=255)
                                          tgGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGaaa                                                                                                            <  1:3228934/150‑1 (MQ=255)
                                           ggCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAAt                                                                                                           >  2:3874430/1‑150 (MQ=255)
                                            gCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATc                                                                                                          >  1:1553119/1‑150 (MQ=255)
                                            gCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATc                                                                                                          <  2:5056915/150‑1 (MQ=255)
                                            gCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATc                                                                                                          <  1:3902083/150‑1 (MQ=255)
                                              gACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCAc                                                                                                        >  1:4924795/1‑150 (MQ=255)
                                                  cAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtc                                                                                                    >  1:1663998/1‑150 (MQ=255)
                                                   aaaaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcg                                                                                                   >  2:150664/1‑150 (MQ=255)
                                                    aaaTATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACcgtcgt                                                                                                  >  2:3607020/1‑150 (MQ=255)
                                                        atCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaaa                                                                                              >  1:1052788/1‑150 (MQ=255)
                                                        atCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTaaa                                                                                              <  1:168306/150‑1 (MQ=255)
                                                         tCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAt                                                                                             <  2:5245624/150‑1 (MQ=255)
                                                           cGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAttt                                                                                           >  2:2772990/1‑150 (MQ=255)
                                                             cTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAACTCACCGTCGTGAAATTTAg                                                                                         >  2:4791157/1‑150 (MQ=255)
                                                             cTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAg                                                                                         >  2:4997003/1‑150 (MQ=255)
                                                             cTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGACCTGCTGTTCGGCACGCAAGAAATCACCGTCGGTAAATTTAg                                                                                         >  2:2014703/1‑150 (MQ=255)
                                                                  gAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGa                                                                                    >  2:2307618/1‑150 (MQ=255)
                                                                         cacaCTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGATAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACg                                                                             <  1:1315065/150‑1 (MQ=255)
                                                                         cacaCTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCCGATCAAACg                                                                             >  2:1191679/1‑150 (MQ=255)
                                                                         cacaCTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACg                                                                             <  2:218065/150‑1 (MQ=255)
                                                                            acTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTTGTCAGATCaaaacgat                                                                          >  1:692129/1‑145 (MQ=255)
                                                                            acTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGAt                                                                          >  1:891057/1‑150 (MQ=255)
                                                                              tGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGATgc                                                                        >  2:1715174/1‑150 (MQ=255)
                                                                              tGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTAAGATCAAAAGGAttc                                                                        >  1:5147924/1‑148 (MQ=255)
                                                                               ggAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATgcg                                                                       <  2:4371733/150‑1 (MQ=255)
                                                                                gAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCTCGCAAGACATCACCGTCGTTAAATTTAGTCAGATCCAACGGATgcgc                                                                      >  2:4657776/1‑150 (MQ=255)
                                                                                 aaGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGcgcg                                                                     <  1:4423817/150‑1 (MQ=255)
                                                                                  aGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCgcgt                                                                    >  2:3870674/1‑150 (MQ=255)
                                                                                   gTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTTAATTTAGTCAGATAAAACGGATGCgcgtg                                                                   >  2:4145570/1‑150 (MQ=255)
                                                                                     aTTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGATGCgcgtgcg                                                                 >  2:1636082/1‑150 (MQ=255)
                                                                                          tgcATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAACGGATGCGCGTGCGTCGcc                                                            >  2:2795602/1‑150 (MQ=255)
                                                                                             aTCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGc                                                         >  2:2458269/1‑150 (MQ=255)
                                                                                                 ggCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCcaca                                                     <  1:1669940/150‑1 (MQ=255)
                                                                                                 ggCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCcaca                                                     <  2:1519606/150‑1 (MQ=255)
                                                                                                   cgcAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAATTATTCATATAAAAAAGATTCGCGTGCGTAGCCAGCCACAGa                                                   >  2:2267386/1‑150 (MQ=255)
                                                                                                     cAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAAAGGATGCGCGTGCGTCGCCGGCCACAGACg                                                 >  1:1214287/1‑150 (MQ=255)
                                                                                                      aGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTTAATTTATTCATAACAAAAGGATTCGCGTGCGTAGCCAGCCACACACGc                                                >  1:2397022/1‑150 (MQ=255)
                                                                                                      aGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCAGCCACAGACGc                                                >  1:2177701/1‑150 (MQ=255)
                                                                                                       gCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGACAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCa                                               <  1:467183/150‑1 (MQ=255)
                                                                                                             tACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCt                                         <  1:5259912/150‑1 (MQ=255)
                                                                                                             tACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTGAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCt                                         <  1:3410615/150‑1 (MQ=255)
                                                                                                              aCCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTg                                        >  1:3100246/1‑150 (MQ=255)
                                                                                                              aCCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAAAGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTg                                        >  2:2620691/1‑150 (MQ=255)
                                                                                                                cGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAACGGATTCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTgcg                                      >  1:3999187/1‑150 (MQ=255)
                                                                                                                 ggCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTAGGCACGCAAGAAATCACCGTCGTTAAATTTAGTAAGTTAAAACGGATGCGCGTGCGTCGCCGGCCCCACACGCACCGGCAgcgc                                     >  1:2362632/1‑150 (MQ=255)
                                                                                                                  gCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgcc                                    <  1:972946/150‑1 (MQ=255)
                                                                                                                   cAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgcca                                   >  2:2873680/1‑150 (MQ=255)
                                                                                                                    aGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAAAGGATGCGCCTGCGTCGCCTGCCCCAGACGCAGCGGCGGcgccac                                  >  2:1023292/1‑150 (MQ=255)
                                                                                                                    aGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAAATTATTAAGATAAAACGGATGCGCGTGCGTCGCCAGCCCCAGACCCAGCGGCTGcgccac                                  >  1:2594934/1‑150 (MQ=255)
                                                                                                                     gTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacg                                 <  2:2675388/150‑1 (MQ=255)
                                                                                                                     gTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacg                                 <  1:2518781/150‑1 (MQ=255)
                                                                                                                     gTATGGTTGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacg                                 <  1:1353347/150‑1 (MQ=255)
                                                                                                                     gTATGGTTGTCGTTTGGATAGAGAACATTGGTAAGTTTTACCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAATCATATAAAAAAGATTCGCGTGCGTAGCCAGACACAAAAGCAGCGGCAGcgccacc                                 >  1:385116/1‑149 (MQ=255)
                                                                                                                       aTGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATCAAAAGGATGCGCGGGCGTAGCCCGCCACAAACGCCCCCGCAGcgccccccc                               >  1:2026962/1‑145 (MQ=255)
                                                                                                                        tGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGcgccacgcca                              >  2:4293355/1‑150 (MQ=255)
                                                                                                                         ggTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCAt                             <  1:2283965/150‑1 (MQ=255)
                                                                                                                           ttGTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTATTCAGATAAAAAGGATGCGCGTGCGTAGCCAGCCACACACGCACCGGCAGcgccacgcccata                           >  1:3271570/1‑146 (MQ=255)
                                                                                                                             gTCGTTTGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACg                         <  2:4590091/150‑1 (MQ=255)
                                                                                                                                 tttGGATAGAGTTCTTTGGTCAGATTTTCCGCATTGATGCACTGCTGTTCTGCACGCAAGAAATCACCGTCGTTAATTTTTGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGAAGCAGCGGCTGCGCCTCGCCATTAAGATAg                     <  2:56799/150‑1 (MQ=255)
                                                                                                                                   tGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTAAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCGGCGCCACACCAATACGATAGca                   >  1:644999/1‑149 (MQ=255)
                                                                                                                                   tGGATAGAGAACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGATCAAACGGAGGCGCGTGCGTCGCCAGCCCCAGACGCAGCGGCTGCGCCCCGCCAGTACGCTAGcc                   >  2:1948792/1‑150 (MQ=255)
                                                                                                                                    ggATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCg                  <  2:3808879/150‑1 (MQ=255)
                                                                                                                                      aTAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGAc                <  1:1961358/150‑1 (MQ=255)
                                                                                                                                      aTAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGAc                <  2:1569835/150‑1 (MQ=255)
                                                                                                                                         gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAAc             <  1:3607141/150‑1 (MQ=255)
                                                                                                                                         gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAAc             <  1:1172770/150‑1 (MQ=255)
                                                                                                                                         gagaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATAAAACGGATGCGCGTGCGTCGCCTGCCCCAGACGCAGCGGCTGCGCCACGCCCATACGATAGCCGACAAc             >  1:5043380/1‑150 (MQ=255)
                                                                                                                                         gagaACTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCAGCCACAGACGCAGCGGCAGCGCCACGCCATTACGATAGCCGACaaa             >  2:1308937/1‑149 (MQ=255)
                                                                                                                                          agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAAca            <  2:4486513/150‑2 (MQ=255)
                                                                                                                                          agaACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACg            <  1:1908955/150‑1 (MQ=255)
                                                                                                                                             aCTTTGGTCAGTTTTTCCGCATTGCTGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACgggg         <  2:3129778/150‑1 (MQ=255)
                                                                                                                                              cTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCAGCCCCAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGga        >  2:664170/1‑150 (MQ=255)
                                                                                                                                              cTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCAATACGATAGCCGACAACGGGga        >  2:254751/1‑150 (MQ=255)
                                                                                                                                              cTTTGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCACATCAAACGGATGCCCGTGCGTCGCCAGCCACAGACGCAGCGGCTGCGCCACCCCAATAAGATAGCCCACACCGGGga        >  1:332334/1‑150 (MQ=255)
                                                                                                                                                ttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGgaga      <  1:4142419/150‑1 (MQ=255)
                                                                                                                                                ttGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTGAAATTTAGTCAGAACAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCCGCGGCTGCGCCACGCCAATACGATAGCCGACAACGGGgaga      >  2:5319758/1‑150 (MQ=255)
                                                                                                                                                ttGGTAAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGgaga      <  2:3445927/150‑1 (MQ=255)
                                                                                                                                                   ttCAGTTTTTCCTCATTGATGCCCTGCTGTTCGGCACGCAAGATATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATcc   <  1:375810/149‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TCGTAATCCGCCAGTTCGTGCAGTTTACGCCCCGCCAGATGATGGCGACGCAAAATATCCGCTACCGAACAGGCACACTGGAAGTATTGCTGCATCAGGCGCAGCTTTTTACCGGCAGTATGGTTGTCGTTTGGATAGAGAACTTTGGTCAGTTTTTCCGCATTGATGCCCTGCTGTTCGGCACGCAAGAAATCACCGTCGTTAAATTTAGTCAGATCAAACGGATGCGCGTGCGTCGCCTGCCACAGACGCAGCGGCTGCGCCACGCCATTACGATAGCCGACAACGGGGAGATCCC  >  CP009273/3544928‑3545225

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A