Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,179,279 A→T 29.9% intergenic (‑4/‑117) rcnR ← / → rcnA transcriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,179,2790AT29.9% 325.9 / 178.9 216intergenic (‑4/‑117)rcnR/rcnAtranscriptional repressor of rcnA/membrane protein conferring nickel and cobalt resistance
Reads supporting (aligned to +/- strand):  ref base A (72/78);  new base T (30/35);  total (102/114)
Fisher's exact test for biased strand distribution p-value = 8.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 216 total aligned reads displayed.

CGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGG  >  CP009273/2179132‑2179427
                                                                                                                                                   |                                                                                                                                                    
cgcgCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAac                                                                                                                                                    <  1:4130970/150‑1 (MQ=255)
 gcgcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTaca                                                                                                                                                   <  2:5224830/150‑1 (MQ=255)
   gcCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACAcc                                                                                                                                                 <  1:3665669/150‑1 (MQ=255)
    ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCt                                                                                                                                                <  1:2060269/150‑1 (MQ=255)
    ccACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCt                                                                                                                                                <  1:299558/150‑1 (MQ=255)
     cACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTg                                                                                                                                               >  1:304680/1‑150 (MQ=255)
      aCGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTgg                                                                                                                                              <  1:3284578/150‑1 (MQ=255)
        ggATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                                                                                            <  1:3656201/150‑1 (MQ=255)
        ggATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                                                                                            >  1:4178372/1‑150 (MQ=255)
        ggATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGtt                                                                                                                                            <  2:1117722/150‑1 (MQ=255)
            agcagcAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACa                                                                                                                                        >  1:2374777/1‑150 (MQ=255)
              cagcaATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAg                                                                                                                                      <  2:4307153/150‑1 (MQ=255)
                  aaTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAata                                                                                                                                  <  1:2000396/150‑1 (MQ=255)
                   aTCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAatat                                                                                                                                 >  2:4597449/1‑150 (MQ=255)
                      tgttgtAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGaa                                                                                                                              >  1:3862139/1‑150 (MQ=255)
                             ataCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGTATCTTACTGGCACGCGCTTTCAGTTTCTGTTTAGCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCag                                                                                                                       <  2:1474752/148‑2 (MQ=255)
                             aaaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCAt                                                                                                                       >  2:2735164/1‑150 (MQ=255)
                              aaCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATa                                                                                                                      <  2:3935661/150‑1 (MQ=255)
                               aCTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAg                                                                                                                     <  2:2326574/150‑1 (MQ=255)
                                cTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGc                                                                                                                    <  1:1743508/150‑1 (MQ=255)
                                 tGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCa                                                                                                                   <  2:3395135/150‑1 (MQ=255)
                                  gCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCAc                                                                                                                  <  1:4481282/150‑1 (MQ=255)
                                   cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATCGCACt                                                                                                                 >  1:4700165/1‑150 (MQ=255)
                                   cAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACt                                                                                                                 <  1:5129679/150‑1 (MQ=255)
                                       gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                                                                                             >  2:3727075/1‑150 (MQ=255)
                                       gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                                                                                             <  2:2981995/150‑1 (MQ=255)
                                       gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                                                                                             <  2:132313/150‑1 (MQ=255)
                                       gcATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTa                                                                                                             <  1:4230351/150‑1 (MQ=255)
                                         aTTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAt                                                                                                           <  1:2135805/150‑1 (MQ=255)
                                         aTTCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAAt                                                                                                           <  1:1508808/150‑1 (MQ=255)
                                          ttCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATc                                                                                                          <  2:1344523/150‑1 (MQ=255)
                                          ttCGTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATc                                                                                                          <  2:3061472/150‑1 (MQ=255)
                                             gTGCGGCTCGTCGAGCTTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTCATGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACTCCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                                                                                                       <  2:2833660/150‑1 (MQ=255)
                                             gTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                                                                                                       <  2:2635796/150‑1 (MQ=255)
                                             gTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                                                                                                       >  1:4434645/1‑150 (MQ=255)
                                             gTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTAc                                                                                                       >  1:2276631/1‑150 (MQ=255)
                                             gTGCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCACAGCACTATTAATCTAc                                                                                                       <  1:1590150/150‑1 (MQ=255)
                                               gCGGCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTg                                                                                                     <  1:512338/150‑1 (MQ=255)
                                                 ggCTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTggg                                                                                                   <  2:2710832/150‑1 (MQ=255)
                                                   cTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTggggg                                                                                                 <  1:134882/150‑1 (MQ=255)
                                                   cTCGTCGAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTggggg                                                                                                 >  1:5144577/1‑150 (MQ=255)
                                                     cgtcgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgt                                                                                               <  1:2835518/150‑1 (MQ=255)
                                                        cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                                                                                            <  1:961307/150‑1 (MQ=255)
                                                        cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                                                                                            <  2:4769837/150‑1 (MQ=255)
                                                        cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                                                                                            <  2:466209/150‑1 (MQ=255)
                                                        cgAGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGgtagt                                                                                            <  1:4662469/150‑1 (MQ=255)
                                                          aGCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTAt                                                                                          >  1:1108424/1‑150 (MQ=255)
                                                           gCATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATc                                                                                         <  1:1453771/150‑1 (MQ=255)
                                                            cATTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCa                                                                                        <  1:2650352/150‑1 (MQ=255)
                                                             aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg                                                                                       >  1:2570272/1‑150 (MQ=255)
                                                             aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg                                                                                       >  1:3335135/1‑150 (MQ=255)
                                                             aTTTTCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg                                                                                       >  1:1279400/1‑150 (MQ=255)
                                                             aTTTTATTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAg                                                                                       <  1:63906/150‑1 (MQ=255)
                                                               tttCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGt                                                                                     <  2:1035514/150‑1 (MQ=255)
                                                                ttCTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTa                                                                                    >  1:4038587/1‑150 (MQ=255)
                                                                  cTTGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACt                                                                                  >  1:4364015/1‑150 (MQ=255)
                                                                    tGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAACATCATAGCACTATTAATCTACTGGGGGGTCGTATCAGGtccggg                                                                                >  2:4397331/1‑145 (MQ=255)
                                                                    tGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgg                                                                                <  2:886279/150‑1 (MQ=255)
                                                                    tGAGCTCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgg                                                                                <  2:2564249/150‑1 (MQ=255)
                                                                        ctccACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTgggggg                                                                            >  1:2526765/3‑150 (MQ=255)
                                                                        ctccACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAAAACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGTGGGGTAGTATCAGGTACTgggggg                                                                            <  1:1313875/148‑1 (MQ=255)
                                                                          ccACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGa                                                                          <  1:1679192/150‑1 (MQ=255)
                                                                           cACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAATAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAg                                                                         <  1:1846822/150‑1 (MQ=255)
                                                                           cACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACGGGGGGGGAg                                                                         >  1:1800153/1‑150 (MQ=255)
                                                                           cACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAg                                                                         <  1:804483/150‑1 (MQ=255)
                                                                              gACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAg                                                                      >  1:4252952/1‑150 (MQ=255)
                                                                                 cTGGCCCTGAATCTTACTGGCACGCGCGTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGTTTAACAATAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGGATCAGGTACTGGGGGGGAGTAGAAt                                                                   <  2:1963191/150‑1 (MQ=255)
                                                                                  tGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATc                                                                  >  1:3643285/1‑150 (MQ=255)
                                                                                  tGGCCCTGAATCTTACTGGCACGCGCTTTCAGTGTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATc                                                                  <  2:3753661/150‑1 (MQ=255)
                                                                                      ccTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATTAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGAt                                                              >  2:4952717/1‑150 (MQ=255)
                                                                                      ccTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGAt                                                              >  1:4249184/1‑150 (MQ=255)
                                                                                      ccTGAATCTCACTGGCACGCGCTTTCAGTTACTGTTTAACACGGATGGTATGAGACCTGCCAACACCTGGTAAACAAGAATATGAAAAATCATAGCACTATTACTCTACGGGGGGGTAGTCTCAGGTACTGGGGGGGAGTAGAATCAGAt                                                              <  2:625405/150‑1 (MQ=255)
                                                                                       cTGACTCTTACGGGCACGCTCTTTCAGTTTCAGTTTATCACCGACTGTATGCGACATGTCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATAAATCTACTGGGGGGTAGTAGCAGGTACTGGGGGGGAGTAGAATCAGAtt                                                             <  2:2466631/150‑1 (MQ=255)
                                                                                       cTGAATCTTCCTGGCACGCGCTTTCAGTTTCAGTTTATCACGGATTGTATGAGACAAGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCCCTATTACTCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGAtt                                                             <  1:3057850/150‑1 (MQ=255)
                                                                                       cTGAATCTTCCAGGCACGCGCTTTCTGGATCTGTTTATCACGGATTGTATGAGACATGGCAACCCCTGGTAAACACGAATATGAAAACTCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGAtt                                                             <  2:4891235/150‑1 (MQ=255)
                                                                                          actCTTACTGGCACGCGCTTTCAGAGTCTGTTTATCACGGATTGTATGAGACACGGCTACACCCGGTTACCAAGAATATGAAAAAACCTAGCACTCTTCATCTACTGGGGGGTAGTATCAGGTACCGGGGGGGAGTAGAATCAGATTGcc                                                          <  2:4677394/148‑1 (MQ=255)
                                                                                          aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGcc                                                          >  1:322533/1‑150 (MQ=255)
                                                                                          aaTCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGcc                                                          >  2:4352044/1‑150 (MQ=255)
                                                                                              ttACTGGCACGCGCTATCAGTTTCTGATTATCACGGATTGTATGAGACAGGCCTACACCCGGTTAACAAGAATATGAAAAATCATAGCACTATTACCCTACTGGGGGGTAGTACCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAAt                                                      <  2:723459/150‑1 (MQ=255)
                                                                                               tACTGGCACGCGCTTTCCCCCTCTGTTTATCCCGGACCGGACGAGACACGGCTACACCCGCCCACCAAGAAAACCAAAAATCCTAGCACTAGTACTCCCCTGGGGGGTAGTAACAGGTACCGGGGGGGAGTAGAATCAGATTGCCGAAtt                                                     <  2:905880/150‑1 (MQ=255)
                                                                                                   ggCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATa                                                 >  1:4017901/1‑150 (MQ=255)
                                                                                                     cACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATaat                                               >  2:4260575/1‑148 (MQ=255)
                                                                                                     cACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGACGAATTAATACt                                               >  2:3433837/1‑150 (MQ=255)
                                                                                                      aCGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTa                                              >  1:4854889/1‑150 (MQ=255)
                                                                                                         cgcTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGa                                           >  2:4466371/1‑150 (MQ=255)
                                                                                                               cAGATTCTGTTTATCACGGATGGGACGAGACATGGCTACACCTGGTTAACAAGAATATGAAAACTCATAGCACTATTAATCGACTGGGGGGTAGTATCCGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAattatt                                     <  2:3263560/150‑1 (MQ=255)
                                                                                                                        tttATAACGGATTGTATGAGACATGTCAACACCTGGTTAACAAGAATAAGAAAAATCATATCAATATTAATCAACTGGGTGGTAGTATCAGATACTTGGGGGGAGTATAATCAGATAGACGAAATAATACTAAGAAATATTATCAtctcc                            >  2:2241814/1‑146 (MQ=255)
                                                                                                                             cACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAAtt                       >  1:2201371/1‑150 (MQ=255)
                                                                                                                                ggATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTAc                    >  2:3483613/1‑150 (MQ=255)
                                                                                                                                 gATTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACa                   >  1:1054835/1‑150 (MQ=255)
                                                                                                                                  aTTGTATGAGACATGGCTACACCTGGTTAACAAGAATATGAAAAATCATAGAACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCTAATTAATACTAAGAATTATTATCATGACCGAATTTACaa                  >  2:2201102/1‑150 (MQ=255)
                                                                                                                                     gTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActc               >  1:576757/1‑150 (MQ=255)
                                                                                                                                      tATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAAATATTATCATGACCGAATTTACAActct              >  1:705450/1‑150 (MQ=255)
                                                                                                                                       aTGAGACAAGGCAACACCCTGACCACAAGAATATGAAAAATCACCGCACCCACCATCTACACGGCGCTACCACCAGGTACATGCCTGTAGGAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtctt             <  2:2113411/150‑1 (MQ=255)
                                                                                                                                        tGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTCCTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAActctac            >  2:4120824/1‑148 (MQ=255)
                                                                                                                                         gagaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACtcttct           >  1:3484095/1‑150 (MQ=255)
                                                                                                                                          cgaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGACTTTACAACTcttctt          >  2:1243489/2‑150 (MQ=255)
                                                                                                                                          agaCATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTcttctt          >  2:3321047/1‑150 (MQ=255)
                                                                                                                                               tGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCa     >  1:4195580/1‑150 (MQ=255)
                                                                                                                                                  cAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCaatg  >  1:56096/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
CGCGCCACGGATAGCAGCAATCTGTTGTAAAACTGCAGCGCATTCGTGCGGCTCGTCGAGCATTTTCTTGAGCGCCACGACCTGGCCCTGAATCTTACTGGCACGCGCTTTCAGTTTCTGTTTATCACGGATTGTATGAGACATGGCAACACCTGGTTAACAAGAATATGAAAAATCATAGCACTATTAATCTACTGGGGGGTAGTATCAGGTACTGGGGGGGAGTAGAATCAGATTGCCGAATTAATACTAAGAATTATTATCATGACCGAATTTACAACTCTTCTTCAGCAAGG  >  CP009273/2179132‑2179427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A