Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,181,645 A→G 32.6% N83N (AAT→AAC)  yehA ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,181,6450AG32.6% 220.4 / 157.6 172N83N (AAT→AAC) yehAputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (68/48);  new base G (29/27);  total (97/75)
Fisher's exact test for biased strand distribution p-value = 4.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG  >  CP009273/2181498‑2181791
                                                                                                                                                   |                                                                                                                                                  
aaTCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                  <  2:2089952/150‑1 (MQ=255)
 atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagttt                                                                                                                                                 <  2:5181618/150‑1 (MQ=255)
 atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagttt                                                                                                                                                 <  2:2604159/150‑1 (MQ=255)
 atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAaattt                                                                                                                                                 <  1:2033889/150‑1 (MQ=255)
      caCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaattt                                                                                                                                            <  1:1535408/150‑1 (MQ=255)
      caCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAaatttatttt                                                                                                                                            >  2:3927089/1‑150 (MQ=255)
        cTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTa                                                                                                                                          <  1:950038/150‑1 (MQ=255)
         tGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTAt                                                                                                                                         <  2:4889439/150‑1 (MQ=255)
           aaCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATgg                                                                                                                                       >  1:4416938/1‑150 (MQ=255)
           aaCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGGCTGAGTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATgg                                                                                                                                       >  2:1955538/1‑150 (MQ=255)
            aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCGAAATGTAAATTTAATTTTATGGt                                                                                                                                      >  2:2373969/1‑150 (MQ=255)
            aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGt                                                                                                                                      >  2:2373910/1‑150 (MQ=255)
            aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGt                                                                                                                                      >  1:633934/1‑150 (MQ=255)
            aCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGt                                                                                                                                      >  2:3796171/1‑150 (MQ=255)
             ccgccgCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTa                                                                                                                                     >  1:727074/1‑150 (MQ=255)
             ccgccgCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTa                                                                                                                                     >  2:935191/1‑150 (MQ=255)
              cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTAt                                                                                                                                    <  2:1424770/150‑1 (MQ=255)
              cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTAt                                                                                                                                    >  1:4367759/1‑150 (MQ=255)
               gccgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATc                                                                                                                                   <  1:3627648/150‑1 (MQ=255)
                 cgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGt                                                                                                                                 <  2:1609858/150‑1 (MQ=255)
                  gccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGtt                                                                                                                                <  2:1842313/150‑1 (MQ=255)
                   ccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTta                                                                                                                               <  1:1397516/150‑1 (MQ=255)
                   ccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTAGTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTta                                                                                                                               >  2:5133497/1‑150 (MQ=255)
                   ccacccGCAGAAACATTCAATTTTTCATTACTGGCCGCAGGTGTCACCGTAACTGAATATCCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTta                                                                                                                               <  1:1397521/147‑1 (MQ=255)
                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                        <  1:2240226/150‑1 (MQ=255)
                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                        >  2:361120/1‑150 (MQ=255)
                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                        >  1:26068/1‑150 (MQ=255)
                            gAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCca                                                                                                                      >  2:4412480/1‑150 (MQ=255)
                             aaaCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaa                                                                                                                     >  2:3586387/1‑150 (MQ=255)
                               aCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCcaaca                                                                                                                   >  2:28496/1‑150 (MQ=255)
                                    cACTTTTGCATGACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                              <  2:452561/150‑1 (MQ=255)
                                            cATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACg                                                                                                      >  2:4695661/1‑150 (MQ=255)
                                             aTTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACgg                                                                                                     >  1:241664/1‑150 (MQ=255)
                                               tACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGc                                                                                                   >  1:2183326/1‑150 (MQ=255)
                                                aCTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCt                                                                                                  <  1:3096220/150‑1 (MQ=255)
                                                    gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATa                                                                                              <  1:2285544/150‑1 (MQ=255)
                                                    gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATa                                                                                              <  2:3654135/150‑1 (MQ=255)
                                                     ccGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAg                                                                                             >  1:5026191/1‑150 (MQ=255)
                                                      cGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgg                                                                                            >  1:1897612/1‑150 (MQ=255)
                                                        cAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgggg                                                                                          >  1:1010199/1‑150 (MQ=255)
                                                        cAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgggg                                                                                          >  1:2952644/1‑150 (MQ=255)
                                                            tgtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGAt                                                                                      >  2:3320319/1‑150 (MQ=255)
                                                               cACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGt                                                                                   >  2:1572520/1‑150 (MQ=255)
                                                                aCCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTa                                                                                  >  2:4713403/1‑150 (MQ=255)
                                                                  cGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAtt                                                                                >  2:910403/1‑150 (MQ=255)
                                                                     aaCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGt                                                                             >  1:1455774/1‑150 (MQ=255)
                                                                      aCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTa                                                                            >  1:1625367/1‑150 (MQ=255)
                                                                      aCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTa                                                                            >  1:1625423/1‑150 (MQ=255)
                                                                      aCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTa                                                                            >  1:4770086/1‑150 (MQ=255)
                                                                       cTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTAc                                                                           >  2:1669581/1‑150 (MQ=255)
                                                                        tGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACt                                                                          <  1:100945/150‑1 (MQ=255)
                                                                        tGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACt                                                                          <  2:770098/150‑1 (MQ=255)
                                                                        agAATAACCACTTGTTAAACTTGTATTCATTTTTTACAACGTTTTTTTGTTTTTATTTGGTCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACt                                                                          <  1:100977/149‑1 (MQ=255)
                                                                           agaaCCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAc                                                                       <  1:2733311/148‑1 (MQ=255)
                                                                           aTAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAc                                                                       >  1:3511172/1‑150 (MQ=255)
                                                                            tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACg                                                                      <  1:2516814/150‑1 (MQ=255)
                                                                                cACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                  <  2:3328721/150‑1 (MQ=255)
                                                                                 aCTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGa                                                                 >  1:914457/1‑150 (MQ=255)
                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                <  1:2482002/150‑1 (MQ=255)
                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                <  2:1106780/150‑1 (MQ=255)
                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                >  2:696756/1‑150 (MQ=255)
                                                                                   tGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACg                                                               <  2:5000346/150‑1 (MQ=255)
                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                              <  2:2910192/150‑1 (MQ=255)
                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                              >  2:2018041/1‑150 (MQ=255)
                                                                                       gAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATc                                                           >  1:4936124/1‑150 (MQ=255)
                                                                                        aaaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCa                                                          >  1:4631955/1‑150 (MQ=255)
                                                                                        aaaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCa                                                          >  2:3239360/1‑150 (MQ=255)
                                                                                         aaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                         >  2:1372631/1‑150 (MQ=255)
                                                                                          aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAtt                                                        <  2:2954245/150‑1 (MQ=255)
                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                      <  1:1470881/150‑1 (MQ=255)
                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                      >  2:1905498/1‑150 (MQ=255)
                                                                                             ggTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaa                                                     >  1:2257795/1‑150 (MQ=255)
                                                                                                       tttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCa                                           <  1:3791128/150‑1 (MQ=255)
                                                                                                        ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCat                                          <  1:4682641/150‑1 (MQ=255)
                                                                                                        ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCat                                          <  2:523691/150‑1 (MQ=255)
                                                                                                        ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCat                                          >  1:59582/1‑150 (MQ=255)
                                                                                                          gACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCatat                                        <  2:4144859/150‑1 (MQ=255)
                                                                                                              aCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCg                                    >  1:3861430/1‑150 (MQ=255)
                                                                                                              aCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCg                                    <  2:4367759/150‑1 (MQ=255)
                                                                                                              aCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCg                                    >  2:3780292/1‑150 (MQ=255)
                                                                                                               cGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGc                                   <  1:1312813/150‑1 (MQ=255)
                                                                                                                     gttgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtg                             <  2:459781/150‑1 (MQ=255)
                                                                                                                     gttgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtg                             <  2:624718/150‑1 (MQ=255)
                                                                                                                          ttttATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGttt                        <  2:3117720/150‑1 (MQ=255)
                                                                                                                           tttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTg                       >  1:5027878/1‑150 (MQ=255)
                                                                                                                              aTTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGt                    >  2:266504/1‑150 (MQ=255)
                                                                                                                               ttGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTa                   >  2:3942855/1‑150 (MQ=255)
                                                                                                                               ttGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATAATTAAAATTCTGCTCATATGTCGCCATTTGTGTTTGAGTa                   >  2:3715528/1‑150 (MQ=255)
                                                                                                                                  gTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGt                >  2:5147207/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa            <  1:1243931/150‑1 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa            >  1:4156802/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa            <  2:1253504/150‑1 (MQ=255)
                                                                                                                                       ttCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaa           >  2:199001/1‑150 (MQ=255)
                                                                                                                                        tCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaaa          >  1:2785425/1‑150 (MQ=255)
                                                                                                                                         cAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAAt         <  1:3454763/150‑1 (MQ=255)
                                                                                                                                         cAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAAt         <  2:794824/150‑1 (MQ=255)
                                                                                                                                           aaaTGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATcc       >  2:60069/1‑150 (MQ=255)
                                                                                                                                            aaTGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCAATTTGTGTTTGAGTAAGTGCAAAATccc      <  2:666604/150‑1 (MQ=255)
                                                                                                                                             aTGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATcccc     >  1:3311764/1‑150 (MQ=255)
                                                                                                                                               gTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCt   <  1:3586387/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG  >  CP009273/2181498‑2181791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A