Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F534 I1 R1	1235	230.2	7611948	95.1%	7238962	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP009273	2,181,645	A→G	N83N (AAT→AAC)	yehA ←	putative fimbrial‑like adhesin protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	2,181,645	0	A	G	99.5%	833.2 / NA	212	N83N (AAT→AAC)	yehA	putative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (101/110); total (102/110)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG  >  CP009273/2181498‑2181791

aaTCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                  <  2:1033613/150‑1 (MQ=255)

aaTCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                  <  2:1128093/150‑1 (MQ=255)

 atcatcACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagttt                                                                                                                                                 <  1:864295/150‑1 (MQ=255)

  tcatcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagttta                                                                                                                                                <  1:3629600/150‑1 (MQ=255)

   tatcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaa                                                                                                                                               >  1:1033886/2‑150 (MQ=255)

   catcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaa                                                                                                                                               >  2:1846190/1‑150 (MQ=255)

    atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                              <  2:1190864/150‑1 (MQ=255)

    atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                              <  2:3714478/150‑1 (MQ=255)

    atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                              <  2:3706360/150‑1 (MQ=255)

    atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                              >  2:3156868/1‑150 (MQ=255)

      caCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaattt                                                                                                                                            >  1:634030/1‑150 (MQ=255)

      caCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaattt                                                                                                                                            <  1:3209513/150‑1 (MQ=255)

      caCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaattt                                                                                                                                            >  1:2433551/1‑150 (MQ=255)

       aCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAAtttt                                                                                                                                           <  1:3568997/150‑1 (MQ=255)

           aaCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATTACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATgg                                                                                                                                       >  2:2491624/1‑150 (MQ=255)

              cgccgcCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTAt                                                                                                                                    <  1:1549984/150‑1 (MQ=255)

               gccgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATc                                                                                                                                   <  1:1718549/150‑1 (MQ=255)

                ccgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCg                                                                                                                                  <  1:3016415/150‑1 (MQ=255)

                  gccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGtt                                                                                                                                >  2:538232/1‑150 (MQ=255)

                    cccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTtat                                                                                                                              <  2:2331399/150‑1 (MQ=255)

                     ccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTtata                                                                                                                             >  1:2448743/1‑150 (MQ=255)

                      cccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATaa                                                                                                                            >  1:588815/1‑150 (MQ=255)

                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                        >  2:3731503/1‑150 (MQ=255)

                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                        >  1:45420/1‑150 (MQ=255)

                            gAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCca                                                                                                                      <  2:2954374/150‑1 (MQ=255)

                             aaaCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaa                                                                                                                     >  2:1798894/1‑150 (MQ=255)

                               aCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaaca                                                                                                                   <  1:1811087/150‑1 (MQ=255)

                                 aTTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaat                                                                                                                 >  2:1395759/1‑150 (MQ=255)

                                  ttCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaata                                                                                                                <  1:268847/150‑1 (MQ=255)

                                   tCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataa                                                                                                               >  1:2341494/1‑150 (MQ=255)

                                   tCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataa                                                                                                               >  1:567893/1‑150 (MQ=255)

                                    cACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                              >  1:2326660/1‑150 (MQ=255)

                                    cACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                              <  2:1476494/150‑1 (MQ=255)

                                      cTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAatat                                                                                                            >  1:293633/1‑150 (MQ=255)

                                       ttttGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATc                                                                                                           <  2:3016718/150‑1 (MQ=255)

                                        tttGCATTACTGGCCGCAGGTTTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATcc                                                                                                          <  1:1223118/150‑1 (MQ=255)

                                            cATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACg                                                                                                      >  1:817956/1‑150 (MQ=255)

                                             aTTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACgg                                                                                                     >  2:319430/1‑150 (MQ=255)

                                               tACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGc                                                                                                   >  1:2571710/1‑150 (MQ=255)

                                                aCTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCt                                                                                                  >  2:3542924/1‑150 (MQ=255)

                                                aCTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCt                                                                                                  >  1:1265432/1‑150 (MQ=255)

                                                      cGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgg                                                                                            <  1:298006/150‑1 (MQ=255)

                                                       gCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAggg                                                                                           >  2:3477111/1‑150 (MQ=255)

                                                       gCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAggg                                                                                           >  1:722488/1‑150 (MQ=255)

                                                        cAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgggg                                                                                          >  2:3382562/1‑150 (MQ=255)

                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                         >  2:1776460/1‑150 (MQ=255)

                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                         >  1:427333/1‑150 (MQ=255)

                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                         <  1:3780274/150‑1 (MQ=255)

                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                         <  1:2701936/150‑1 (MQ=255)

                                                           gtgtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGa                                                                                       <  1:207079/150‑1 (MQ=255)

                                                             gtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATa                                                                                     <  1:262072/150‑1 (MQ=255)

                                                               cACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGt                                                                                   >  2:2172158/1‑150 (MQ=255)

                                                               cACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGt                                                                                   <  2:1070636/150‑1 (MQ=255)

                                                                  cGTAACTGAATAACCACTGGTGACACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAtt                                                                                <  2:2075461/150‑1 (MQ=255)

                                                                   gTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAttt                                                                               >  2:1627616/1‑150 (MQ=255)

                                                                    tAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTg                                                                              >  1:2378504/1‑150 (MQ=255)

                                                                     aaCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGt                                                                             >  2:2207812/1‑150 (MQ=255)

                                                                             aaCCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGc                                                                     >  1:1089461/1‑150 (MQ=255)

                                                                               ccACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAt                                                                   <  2:2917345/150‑1 (MQ=255)

                                                                               ccACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAt                                                                   <  2:3049270/150‑1 (MQ=255)

                                                                                cACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATg                                                                  <  2:3507318/150‑1 (MQ=255)

                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                <  2:2716160/150‑1 (MQ=255)

                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                              >  1:3339912/1‑150 (MQ=255)

                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                              >  1:32307/1‑150 (MQ=255)

                                                                                      tGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAAt                                                            <  2:817956/150‑1 (MQ=255)

                                                                                      tGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAAt                                                            <  1:311168/150‑1 (MQ=255)

                                                                                         aaCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                         <  2:3644825/150‑1 (MQ=255)

                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                      <  1:2019406/150‑1 (MQ=255)

                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                      >  2:2803988/1‑150 (MQ=255)

                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                      >  2:288898/1‑150 (MQ=255)

                                                                                               tCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAt                                                   <  1:3152698/150‑1 (MQ=255)

                                                                                                cTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAtt                                                  <  2:2677296/150‑1 (MQ=255)

                                                                                                  gATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTAt                                                <  2:739249/150‑1 (MQ=255)

                                                                                                     tattTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCt                                             <  2:3786898/150‑1 (MQ=255)

                                                                                                      attTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                            >  2:418407/1‑150 (MQ=255)

                                                                                                      attTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                            <  1:3731503/150‑1 (MQ=255)

                                                                                                        ttGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCat                                          >  2:1535681/1‑150 (MQ=255)

                                                                                                          gACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCatat                                        <  1:1036976/150‑1 (MQ=255)

                                                                                                          gACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCatat                                        >  1:2111970/1‑150 (MQ=255)

                                                                                                           aCAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGTGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATg                                       <  1:3219724/150‑1 (MQ=255)

                                                                                                            cAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGt                                      <  2:588815/150‑1 (MQ=255)

                                                                                                              aCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCg                                    <  1:1287420/150‑1 (MQ=255)

                                                                                                                gTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGcc                                  >  2:1928802/1‑150 (MQ=255)

                                                                                                                 tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTATGCATGACGAATCATTAAAATTATGCTCATATGTCGCCa                                 <  1:2529611/150‑1 (MQ=255)

                                                                                                                 tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCa                                 >  1:774517/1‑150 (MQ=255)

                                                                                                                 tGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCa                                 <  1:781369/150‑1 (MQ=255)

                                                                                                                   aTGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAtt                               >  2:3399859/1‑150 (MQ=255)

                                                                                                                   aTGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAtt                               <  1:664763/150‑1 (MQ=255)

                                                                                                                        gttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTgtgt                          >  2:701039/1‑150 (MQ=255)

                                                                                                                        gttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTgtgt                          <  2:691286/150‑1 (MQ=255)

                                                                                                                          ttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGttt                        >  2:265571/1‑150 (MQ=255)

                                                                                                                          ttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGttt                        >  1:2774769/1‑150 (MQ=255)

                                                                                                                            ttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGa                      >  2:2577428/1‑150 (MQ=255)

                                                                                                                              aTTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGt                    >  2:2752428/1‑150 (MQ=255)

                                                                                                                                tGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                  >  1:3414435/1‑150 (MQ=255)

                                                                                                                                  gTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGt                <  2:3093467/150‑1 (MQ=255)

                                                                                                                                     cATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCa             <  1:2669029/150‑1 (MQ=255)

                                                                                                                                       ttCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaaa           <  2:2399802/150‑1 (MQ=255)

                                                                                                                                              tNTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccccc    >  1:2287840/1‑150 (MQ=255)

                                                                                                                                              tGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccccc    <  1:2550397/150‑1 (MQ=255)

AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG  >  CP009273/2181498‑2181791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGA  >  CP009273/2181497‑2181792

TAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGT                                                                                                                                                    >  SRR19973061.2770175/1‑150 (MQ=60)

TAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGT                                                                                                                                                    >  SRR19973061.2769405/1‑150 (MQ=60)

TAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGT                                                                                                                                                    <  SRR19973061.2301973/150‑1 (MQ=60)

  ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTT                                                                                                                                                  <  SRR19973061.866535/150‑1 (MQ=60)

  ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTT                                                                                                                                                  >  SRR19973061.2405926/1‑150 (MQ=60)

   TCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTA                                                                                                                                                 <  SRR19973061.3638882/150‑1 (MQ=60)

    TATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAA                                                                                                                                                >  SRR19973061.1036546/1‑150 (MQ=60)

    TATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAA                                                                                                                                                >  SRR19973061.1036536/1‑150 (MQ=60)

    CATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAA                                                                                                                                                >  SRR19973061.2924833/1‑150 (MQ=60)

     ATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAAT                                                                                                                                               <  SRR19973061.3635047/150‑1 (MQ=60)

       CACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTT                                                                                                                                             >  SRR19973061.635643/1‑150 (MQ=60)

       CACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTT                                                                                                                                             >  SRR19973061.2439760/1‑150 (MQ=60)

       CACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTT                                                                                                                                             <  SRR19973061.3217761/150‑1 (MQ=60)

        ACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTT                                                                                                                                            <  SRR19973061.3578141/150‑1 (MQ=60)

         CTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTA                                                                                                                                           >  SRR19973061.3222831/1‑150 (MQ=60)

         CTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTA                                                                                                                                           >  SRR19973061.444616/1‑150 (MQ=60)

          TGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTAT                                                                                                                                          >  SRR19973061.2576088/1‑150 (MQ=60)

          TGAACCGCCGCCCCCCGCAGAAACATTCACTTGTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTAT                                                                                                                                          <  SRR19973061.2095852/150‑1 (MQ=60)

            AACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGG                                                                                                                                        >  SRR19973061.3222592/1‑150 (MQ=60)

               CGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTAT                                                                                                                                     <  SRR19973061.1553838/150‑1 (MQ=60)

                GCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATC                                                                                                                                    <  SRR19973061.1722856/150‑1 (MQ=60)

                 CCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCG                                                                                                                                   <  SRR19973061.3024203/150‑1 (MQ=60)

                   GCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTT                                                                                                                                 <  SRR19973061.3333578/150‑1 (MQ=60)

                   GCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTT                                                                                                                                 <  SRR19973061.2341581/150‑1 (MQ=60)

                    CCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTA                                                                                                                                <  SRR19973061.3142135/150‑1 (MQ=60)

                    CCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTA                                                                                                                                <  SRR19973061.1149452/150‑1 (MQ=60)

                     CCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTAT                                                                                                                               <  SRR19973061.2323562/150‑1 (MQ=60)

                      CCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATA                                                                                                                              >  SRR19973061.2454987/1‑150 (MQ=60)

                       CCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAA                                                                                                                             >  SRR19973061.590349/1‑150 (MQ=60)

                         CGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAG                                                                                                                           <  SRR19973061.2445750/150‑1 (MQ=60)

                           CAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTC                                                                                                                         >  SRR19973061.45591/1‑150 (MQ=60)

                            AGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCC                                                                                                                        >  SRR19973061.129114/1‑150 (MQ=60)

                             GAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCA                                                                                                                       <  SRR19973061.2387499/150‑1 (MQ=60)

                                ACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACA                                                                                                                    <  SRR19973061.1815616/150‑1 (MQ=60)

                                   TTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATA                                                                                                                 <  SRR19973061.269619/150‑1 (MQ=60)

                                   TTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATA                                                                                                                 <  SRR19973061.269586/150‑1 (MQ=60)

                                   TTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATA                                                                                                                 <  SRR19973061.2989363/150‑1 (MQ=60)

                                    TCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAA                                                                                                                >  SRR19973061.2347442/1‑150 (MQ=60)

                                    TCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAA                                                                                                                >  SRR19973061.569478/1‑150 (MQ=60)

                                    TCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAA                                                                                                                >  SRR19973061.569378/1‑150 (MQ=60)

                                    TCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAA                                                                                                                >  SRR19973061.2479239/1‑150 (MQ=60)

                                     CACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAAT                                                                                                               >  SRR19973061.2332563/1‑150 (MQ=60)

                                       CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATAT                                                                                                             >  SRR19973061.294419/1‑150 (MQ=60)

                                        TTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATC                                                                                                            <  SRR19973061.2366660/150‑1 (MQ=60)

                                         TTTGCATTTCTGGCCGCAGGTGTTACCGTAACTGTATAACCACTGGTGTAACTGGTTTGTTTATTTGTCAACGTTTTGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                           <  SRR19973061.1228194/150‑1 (MQ=60)

                                         TTTGCATTACTGGCCGCAGGTTTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                           <  SRR19973061.1226168/150‑1 (MQ=60)

                                         TTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                           >  SRR19973061.69789/1‑150 (MQ=60)

                                         TTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                           <  SRR19973061.2636626/150‑1 (MQ=60)

                                             CATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACG                                                                                                       >  SRR19973061.820094/1‑150 (MQ=60)

                                                TACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGC                                                                                                    >  SRR19973061.2578291/1‑150 (MQ=60)

                                                 ACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCT                                                                                                   >  SRR19973061.1268557/1‑150 (MQ=60)

                                                   TGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCA                                                                                                 >  SRR19973061.590251/1‑150 (MQ=60)

                                                    GGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCAT                                                                                                <  SRR19973061.1220969/150‑1 (MQ=60)

                                                       CGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGG                                                                                             <  SRR19973061.298800/150‑1 (MQ=60)

                                                        GCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGG                                                                                            <  SRR19973061.1550725/150‑1 (MQ=60)

                                                        GCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGG                                                                                            <  SRR19973061.1551177/150‑1 (MQ=60)

                                                        GCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGG                                                                                            >  SRR19973061.724375/1‑150 (MQ=60)

                                                        GCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGG                                                                                            <  SRR19973061.709042/150‑1 (MQ=60)

                                                          AGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGT                                                                                          <  SRR19973061.2693889/150‑1 (MQ=60)

                                                          AGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGT                                                                                          >  SRR19973061.428493/1‑150 (MQ=60)

                                                          AGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGT                                                                                          <  SRR19973061.2708884/150‑1 (MQ=60)

                                                          AGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGT                                                                                          <  SRR19973061.798571/150‑1 (MQ=60)

                                                          AGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGT                                                                                          <  SRR19973061.3789918/150‑1 (MQ=60)

                                                            GTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGA                                                                                        <  SRR19973061.207697/150‑1 (MQ=60)

                                                             TGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGAT                                                                                       <  SRR19973061.3757404/150‑1 (MQ=60)

                                                              GTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATA                                                                                      <  SRR19973061.262785/150‑1 (MQ=60)

                                                               TCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAG                                                                                     >  SRR19973061.1079890/1‑150 (MQ=60)

                                                                  CCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTAT                                                                                  <  SRR19973061.838167/150‑1 (MQ=60)

                                                                   CGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATT                                                                                 <  SRR19973061.2525514/150‑1 (MQ=60)

                                                                   CGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATT                                                                                 <  SRR19973061.384981/150‑1 (MQ=60)

                                                                   CGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATT                                                                                 <  SRR19973061.613771/150‑1 (MQ=60)

                                                                    GTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTT                                                                                >  SRR19973061.660773/1‑150 (MQ=60)

                                                                     TAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTG                                                                               >  SRR19973061.2384560/1‑150 (MQ=60)

                                                                      AACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGT                                                                              >  SRR19973061.194590/1‑150 (MQ=60)

                                                                              AACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGC                                                                      >  SRR19973061.1092254/1‑150 (MQ=60)

                                                                              AACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGC                                                                      >  SRR19973061.3577583/1‑150 (MQ=60)

                                                                                CCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAT                                                                    <  SRR19973061.22959/150‑1 (MQ=60)

                                                                                    TGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACG                                                                >  SRR19973061.2727318/1‑150 (MQ=60)

                                                                                     GGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGA                                                               >  SRR19973061.32425/1‑150 (MQ=60)

                                                                                     GGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGA                                                               >  SRR19973061.3348510/1‑150 (MQ=60)

                                                                                     GGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGA                                                               >  SRR19973061.3348571/1‑150 (MQ=60)

                                                                                       TGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAAT                                                             <  SRR19973061.311985/150‑1 (MQ=60)

                                                                                       TGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAAT                                                             >  SRR19973061.337174/1‑150 (MQ=60)

                                                                                       TGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAAT                                                             <  SRR19973061.311568/150‑1 (MQ=60)

                                                                                          AACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAT                                                          >  SRR19973061.1818944/1‑150 (MQ=60)

                                                                                            CTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTAACGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTA                                                        >  SRR19973061.1577787/1‑150 (MQ=60)

                                                                                             TGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAA                                                       <  SRR19973061.2024500/150‑1 (MQ=60)

                                                                                             TGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAA                                                       <  SRR19973061.2024480/150‑1 (MQ=60)

                                                                                                TCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAAT                                                    <  SRR19973061.3160804/150‑1 (MQ=60)

                                                                                                   GATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTAT                                                 <  SRR19973061.2569907/150‑1 (MQ=60)

                                                                                                       ATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTC                                             <  SRR19973061.3741048/150‑1 (MQ=60)

                                                                                                         TTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCAT                                           <  SRR19973061.3213768/150‑1 (MQ=60)

                                                                                                          TGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATA                                          <  SRR19973061.3075682/150‑1 (MQ=60)

                                                                                                           GACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATAT                                         >  SRR19973061.2117269/1‑150 (MQ=60)

                                                                                                           GACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATAT                                         <  SRR19973061.1039634/150‑1 (MQ=60)

                                                                                                            ACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGTGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATG                                        <  SRR19973061.3228005/150‑1 (MQ=60)

                                                                                                              AACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTC                                      >  SRR19973061.403655/1‑150 (MQ=60)

                                                                                                               ACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCG                                     <  SRR19973061.1290609/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTATGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  <  SRR19973061.2536057/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTATGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  <  SRR19973061.2536953/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  >  SRR19973061.3675759/1‑150 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  <  SRR19973061.783446/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  <  SRR19973061.783421/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  <  SRR19973061.783274/150‑1 (MQ=60)

                                                                                                                  TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                  >  SRR19973061.776555/1‑150 (MQ=60)

                                                                                                                   GATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAT                                 >  SRR19973061.2810345/1‑150 (MQ=60)

                                                                                                                    ATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATT                                <  SRR19973061.666474/150‑1 (MQ=60)

                                                                                                                       TTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGT                             <  SRR19973061.2949346/150‑1 (MQ=60)

                                                                                                                           TTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTT                         >  SRR19973061.2781909/1‑150 (MQ=60)

                                                                                                                              TATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAG                      <  SRR19973061.2262334/150‑1 (MQ=60)

                                                                                                                                TTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTA                    >  SRR19973061.885348/1‑150 (MQ=60)

                                                                                                                                 TGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAA                   >  SRR19973061.3423201/1‑150 (MQ=60)

                                                                                                                                    TCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTG                <  SRR19973061.539652/150‑1 (MQ=60)

                                                                                                                                     CCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGC               >  SRR19973061.2361608/1‑150 (MQ=60)

                                                                                                                                      CATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCA              <  SRR19973061.2675895/150‑1 (MQ=60)

                                                                                                                                           AAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATC         >  SRR19973061.2682850/1‑150 (MQ=60)

                                                                                                                                            AAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCC        >  SRR19973061.323699/1‑150 (MQ=60)

                                                                                                                                            AAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCC        <  SRR19973061.2584028/150‑1 (MQ=60)

                                                                                                                                             AATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCC       >  SRR19973061.744239/1‑150 (MQ=60)

                                                                                                                                             AATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCC       >  SRR19973061.3673151/1‑150 (MQ=60)

                                                                                                                                               TNTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCC     >  SRR19973061.2293627/1‑150 (MQ=60)

                                                                                                                                               TGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCC     <  SRR19973061.2556889/150‑1 (MQ=60)

                                                                                                                                                 TAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG   <  SRR19973061.1799940/150‑1 (MQ=60)

                                                                                                                                                  AAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGA  >  SRR19973061.1504509/1‑150 (MQ=60)

                                                                                                                                                  AAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGA  <  SRR19973061.1645479/150‑1 (MQ=60)

TAATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGA  >  CP009273/2181497‑2181792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`