Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,181,645 A→G 100% N83N (AAT→AAC)  yehA ← putative fimbrial‑like adhesin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,181,6450AG99.7% 1344.1 / ‑5.1 338N83N (AAT→AAC) yehAputative fimbrial‑like adhesin protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (150/186);  total (151/187)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 358 total aligned reads displayed.

AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  CP009273/2181498‑2181794
                                                                                                                                                   |                                                                                                                                                     
aaTCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtt                                                                                                                                                     <  1:2694477/150‑1 (MQ=255)
    atcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaat                                                                                                                                                 >  1:4915806/1‑150 (MQ=255)
     tcaCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAagtttaatt                                                                                                                                                <  2:3184610/150‑1 (MQ=255)
       aCTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAAtttt                                                                                                                                              <  1:363541/150‑1 (MQ=255)
        cTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTa                                                                                                                                             <  1:2309982/150‑1 (MQ=255)
           aaCCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATgg                                                                                                                                          >  1:1103805/1‑150 (MQ=255)
                 cgccCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGt                                                                                                                                    <  2:4351155/150‑1 (MQ=255)
                   ccccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTta                                                                                                                                  <  2:714579/150‑1 (MQ=255)
                    cccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTtat                                                                                                                                 >  2:1573252/1‑150 (MQ=255)
                    cccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTtat                                                                                                                                 <  2:202151/150‑1 (MQ=255)
                    cccccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTtat                                                                                                                                 <  2:3272023/150‑1 (MQ=255)
                      cccGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATaa                                                                                                                               >  1:1304139/1‑150 (MQ=255)
                        cGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAg                                                                                                                             <  1:1726281/150‑1 (MQ=255)
                          cAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTc                                                                                                                           <  1:2180374/150‑1 (MQ=255)
                           aGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTcc                                                                                                                          >  2:3477186/1‑150 (MQ=255)
                            gAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCca                                                                                                                         <  1:3034210/150‑1 (MQ=255)
                             aaaCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaa                                                                                                                        >  2:3409039/1‑150 (MQ=255)
                               aCATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaaca                                                                                                                      >  2:176005/1‑150 (MQ=255)
                               aCATTCAATTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTTTCGTTATAAAGTCcaaca                                                                                                                      <  1:2027723/150‑1 (MQ=255)
                                cATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaacaa                                                                                                                     >  2:2550009/1‑150 (MQ=255)
                                cATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCcaacaa                                                                                                                     >  2:4722056/1‑150 (MQ=255)
                                 aTTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaat                                                                                                                    <  2:4654954/150‑1 (MQ=255)
                                  ttCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaata                                                                                                                   <  1:1192665/150‑1 (MQ=255)
                                    cACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                                 >  2:296420/1‑150 (MQ=255)
                                    cACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACaataat                                                                                                                 <  1:2938305/150‑1 (MQ=255)
                                        tttGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATcc                                                                                                             <  1:4160378/150‑1 (MQ=255)
                                         ttGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCg                                                                                                            <  1:2778424/150‑1 (MQ=255)
                                         ttGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCg                                                                                                            <  2:872570/150‑1 (MQ=255)
                                           gCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGAc                                                                                                          <  1:2095425/150‑1 (MQ=255)
                                             aTTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACgg                                                                                                        >  1:4489177/1‑150 (MQ=255)
                                              ttACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACggg                                                                                                       <  1:3535550/150‑1 (MQ=255)
                                              taaCTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGACCATTCAAAATGTAAGATTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACggg                                                                                                       >  1:869382/3‑150 (MQ=255)
                                               tACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGc                                                                                                      >  2:2000255/1‑150 (MQ=255)
                                                 cTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTc                                                                                                    <  1:165078/150‑1 (MQ=255)
                                                   ggCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCAt                                                                                                  <  2:1461694/150‑1 (MQ=255)
                                                   ggCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCAt                                                                                                  >  1:289431/1‑150 (MQ=255)
                                                    gCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATa                                                                                                 >  1:3759287/1‑150 (MQ=255)
                                                     ccGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAg                                                                                                <  1:1930904/150‑1 (MQ=255)
                                                     ccGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAg                                                                                                <  1:697133/150‑1 (MQ=255)
                                                      cGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAgg                                                                                               <  1:1319487/150‑1 (MQ=255)
                                                        cAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTTAAGTTGTTTTTATTGGTCAATTCAAAATGTAAGTTTAAGTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCAAAggtg                                                                                             >  2:3106197/1‑148 (MQ=255)
                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                            >  2:2516227/1‑150 (MQ=255)
                                                         aGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGt                                                                                            >  2:4253223/1‑150 (MQ=255)
                                                             gtCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATa                                                                                        <  1:410243/150‑1 (MQ=255)
                                                                    tAACTGAATAACCACAGGTGAAACTGGTCTGATTATATGACAACGTGATGTTGTTTTTATTGGTCCATACAAAATGTAAGTTTAATTTTATGGTATCTTTATAAAGTCCAACAATAATATCCCACGAGCTCATATGGGAGATAGTAtttt                                                                                 >  2:3898027/1‑149 (MQ=255)
                                                                     aaCTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGt                                                                                <  2:289969/150‑1 (MQ=255)
                                                                         gAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACtt                                                                            <  2:3930709/150‑1 (MQ=255)
                                                                          aaTAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTa                                                                           <  1:1410123/150‑1 (MQ=255)
                                                                            tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACg                                                                         <  2:3832048/150‑1 (MQ=255)
                                                                            tAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACg                                                                         <  1:1875715/150‑1 (MQ=255)
                                                                               ccACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAt                                                                      <  1:2000255/150‑1 (MQ=255)
                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                   <  2:3214346/150‑1 (MQ=255)
                                                                                  cTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAc                                                                   >  1:918856/1‑150 (MQ=255)
                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                                 >  1:2356321/1‑150 (MQ=255)
                                                                                    ggTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGa                                                                 <  2:4844130/150‑1 (MQ=255)
                                                                                         aaCTGGTCTGATTTTTTGACAAAGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAt                                                            <  1:2508114/150‑1 (MQ=255)
                                                                                          aCTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAtt                                                           <  2:2191811/150‑1 (MQ=255)
                                                                                           cTGGTCTGATTTTTTGACATCGTGATGTTGTTTTTATTGGTCCATTCAAATTGTTAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTa                                                          <  1:3166170/150‑1 (MQ=255)
                                                                                           cTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTa                                                          <  1:1601664/150‑1 (MQ=255)
                                                                                            tGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaa                                                         >  1:4406230/1‑150 (MQ=255)
                                                                                             ggTCTGATTATTTGACAACGTGATCTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTaaa                                                        >  2:2775453/1‑150 (MQ=255)
                                                                                                    ttattTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGc                                                 <  2:1142710/150‑1 (MQ=255)
                                                                                                      attTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                               >  2:3357466/1‑150 (MQ=255)
                                                                                                      attTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTc                                               <  1:3495235/150‑1 (MQ=255)
                                                                                                           aCAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATg                                          <  1:4560407/150‑1 (MQ=255)
                                                                                                            cAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGt                                         >  1:1924897/1‑150 (MQ=255)
                                                                                                            cAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGt                                         >  2:509094/1‑150 (MQ=255)
                                                                                                              aCGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCg                                       <  2:1532862/150‑1 (MQ=255)
                                                                                                                  gATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAt                                   <  1:829500/150‑1 (MQ=255)
                                                                                                                    tgttgtTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAttt                                 <  1:4838655/150‑1 (MQ=255)
                                                                                                                    tgttgtTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAttt                                 <  1:5199431/150‑1 (MQ=255)
                                                                                                                      ttgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgt                               >  2:829913/1‑150 (MQ=255)
                                                                                                                       tgttTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTtgtg                              >  2:5294650/1‑150 (MQ=255)
                                                                                                                         tttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                            <  1:3241602/150‑1 (MQ=255)
                                                                                                                         tttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGtt                            <  2:5186903/150‑1 (MQ=255)
                                                                                                                          ttttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGttt                           <  2:1934578/150‑1 (MQ=255)
                                                                                                                           tttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTg                          >  1:3929695/1‑150 (MQ=255)
                                                                                                                           tttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTg                          >  1:1034234/1‑150 (MQ=255)
                                                                                                                            ttATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGa                         <  2:1230206/150‑1 (MQ=255)
                                                                                                                              aTTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAGTTGTGTTTGAGt                       >  2:4383111/1‑150 (MQ=255)
                                                                                                                              aTTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTAGGCTCATATGTCGCCAGTTGTGTTTGAGt                       >  2:1082250/1‑150 (MQ=255)
                                                                                                                                tGGTCCATTCAAAATGTAATTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                     <  1:1791724/150‑1 (MQ=255)
                                                                                                                                tGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTaa                     <  2:4494161/150‑1 (MQ=255)
                                                                                                                                 ggTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAg                    >  1:3892898/1‑150 (MQ=255)
                                                                                                                                    ccATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGc                 >  2:401260/1‑150 (MQ=255)
                                                                                                                                     cATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCa                >  2:3613641/1‑150 (MQ=255)
                                                                                                                                     cATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCa                >  2:2463756/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa               >  1:5133586/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa               >  2:2307283/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa               >  2:4753250/1‑150 (MQ=255)
                                                                                                                                      aTTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCaa               >  1:1004187/1‑150 (MQ=255)
                                                                                                                                          aaaaTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATc           >  1:2378641/1‑150 (MQ=255)
                                                                                                                                          aaaaTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATc           <  1:3828699/150‑1 (MQ=255)
                                                                                                                                            aaTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccc         >  2:5228494/1‑150 (MQ=255)
                                                                                                                                             aTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATcccc        >  1:2529004/1‑150 (MQ=255)
                                                                                                                                             aTGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATGTGTGTTTGAGTAAGTGCAAAATcccc        >  2:1709600/1‑150 (MQ=255)
                                                                                                                                              tGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATccccc       <  2:4954128/150‑1 (MQ=255)
                                                                                                                                               gTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCt      <  1:561807/150‑1 (MQ=255)
                                                                                                                                               gTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCt      <  2:4131701/150‑1 (MQ=255)
                                                                                                                                                tAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTg     <  1:503894/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  CP009273/2181498‑2181794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  CP009273/2181498‑2181794
                                                                                                                                                   |                                                                                                                                                     
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTT                                                                                                                                                     >  SRR19973062.537025/1‑150 (MQ=60)
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTT                                                                                                                                                     <  SRR19973062.2708590/150‑1 (MQ=60)
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTT                                                                                                                                                     <  SRR19973062.3278653/150‑1 (MQ=60)
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTT                                                                                                                                                     >  SRR19973062.2240176/1‑150 (MQ=60)
 ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTT                                                                                                                                                    <  SRR19973062.1908497/150‑1 (MQ=60)
 ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTT                                                                                                                                                    <  SRR19973062.834402/150‑1 (MQ=60)
 ATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTT                                                                                                                                                    >  SRR19973062.2197188/1‑150 (MQ=60)
  TCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTA                                                                                                                                                   <  SRR19973062.1802745/150‑1 (MQ=60)
  TCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTA                                                                                                                                                   <  SRR19973062.1802710/150‑1 (MQ=60)
    ATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAAT                                                                                                                                                 >  SRR19973062.4941313/1‑150 (MQ=60)
    ATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAAT                                                                                                                                                 >  SRR19973062.4153091/1‑150 (MQ=60)
     TCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATT                                                                                                                                                <  SRR19973062.5097197/150‑1 (MQ=60)
       ACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTT                                                                                                                                              <  SRR19973062.365176/150‑1 (MQ=60)
       ACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTT                                                                                                                                              <  SRR19973062.1102808/150‑1 (MQ=60)
        CTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTA                                                                                                                                             <  SRR19973062.2322121/150‑1 (MQ=60)
        CTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTA                                                                                                                                             <  SRR19973062.2140272/150‑1 (MQ=60)
         TGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTAT                                                                                                                                            <  SRR19973062.1068017/150‑1 (MQ=60)
          GAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATG                                                                                                                                           <  SRR19973062.4122918/150‑1 (MQ=60)
          GAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATG                                                                                                                                           <  SRR19973062.2106111/150‑1 (MQ=60)
           AACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGG                                                                                                                                          <  SRR19973062.3926291/150‑1 (MQ=60)
           AACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGG                                                                                                                                          >  SRR19973062.1108995/1‑150 (MQ=60)
             CCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTA                                                                                                                                        >  SRR19973062.479940/1‑150 (MQ=60)
                 CGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGT                                                                                                                                    >  SRR19973062.3716147/1‑150 (MQ=60)
                 CGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGT                                                                                                                                    >  SRR19973062.1932799/1‑150 (MQ=60)
                  GCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTT                                                                                                                                   <  SRR19973062.3791388/150‑1 (MQ=60)
                   CCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTA                                                                                                                                  >  SRR19973062.3530100/1‑150 (MQ=60)
                    CCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTAT                                                                                                                                 <  SRR19973062.5123475/150‑1 (MQ=60)
                     CCCCGCATAAACATTCACTTTTGCATTTCTGGCCGCCGGTGTCACCGTAACTGAATAACCCCTGGTGAAACTGGTCTGATTATTTGTCAACGTGCTGTTGTTTTTATTGGTCCATTCAAAATGTTAGTTTTATTTTATGGTATCGTTATA                                                                                                                                <  SRR19973062.3016462/150‑1 (MQ=60)
                     CCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATA                                                                                                                                <  SRR19973062.5206658/150‑1 (MQ=60)
                     CCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATA                                                                                                                                >  SRR19973062.3681813/1‑150 (MQ=60)
                      CCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAA                                                                                                                               >  SRR19973062.3561390/1‑150 (MQ=60)
                      CCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAA                                                                                                                               >  SRR19973062.1310107/1‑150 (MQ=60)
                        CGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAG                                                                                                                             <  SRR19973062.1734694/150‑1 (MQ=60)
                        CGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAG                                                                                                                             <  SRR19973062.1734715/150‑1 (MQ=60)
                          CAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTC                                                                                                                           <  SRR19973062.2191355/150‑1 (MQ=60)
                            GAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCA                                                                                                                         <  SRR19973062.3050013/150‑1 (MQ=60)
                             AAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAA                                                                                                                        >  SRR19973062.2366638/1‑150 (MQ=60)
                              AACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAAC                                                                                                                       >  SRR19973062.1121854/1‑150 (MQ=60)
                               ACATTCAATTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTTTCGTTATAAAGTCCAACA                                                                                                                      <  SRR19973062.2038140/150‑1 (MQ=60)
                                 ATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAAT                                                                                                                    >  SRR19973062.3860129/1‑150 (MQ=60)
                                 ATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAAT                                                                                                                    >  SRR19973062.3231038/1‑150 (MQ=60)
                                 ATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAAT                                                                                                                    >  SRR19973062.3230976/1‑150 (MQ=60)
                                  TTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATA                                                                                                                   <  SRR19973062.1198240/150‑1 (MQ=60)
                                    CACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAAT                                                                                                                 <  SRR19973062.1288515/150‑1 (MQ=60)
                                    CACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAAT                                                                                                                 <  SRR19973062.2953765/150‑1 (MQ=60)
                                      CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAATATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATAT                                                                                                               <  SRR19973062.4873339/150‑1 (MQ=60)
                                      CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATAT                                                                                                               <  SRR19973062.2891697/150‑1 (MQ=60)
                                      CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATAT                                                                                                               >  SRR19973062.1220815/1‑150 (MQ=60)
                                      CTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATAT                                                                                                               <  SRR19973062.484483/150‑1 (MQ=60)
                                       TTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATC                                                                                                              <  SRR19973062.2565924/150‑1 (MQ=60)
                                        TTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                             <  SRR19973062.3577038/150‑1 (MQ=60)
                                        TTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCC                                                                                                             <  SRR19973062.4181958/150‑1 (MQ=60)
                                         TTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCG                                                                                                            <  SRR19973062.2793102/150‑1 (MQ=60)
                                           GCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGAC                                                                                                          <  SRR19973062.2036657/150‑1 (MQ=60)
                                           GCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGAC                                                                                                          <  SRR19973062.2106133/150‑1 (MQ=60)
                                           GCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGAC                                                                                                          >  SRR19973062.920969/1‑150 (MQ=60)
                                             ATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGG                                                                                                        >  SRR19973062.4512320/1‑150 (MQ=60)
                                             ATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGG                                                                                                        >  SRR19973062.52849/1‑150 (MQ=60)
                                             ATTACTGGCCGAAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGG                                                                                                        >  SRR19973062.2749392/1‑150 (MQ=60)
                                              TTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGTTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGG                                                                                                       >  SRR19973062.873088/1‑150 (MQ=60)
                                              TTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGG                                                                                                       <  SRR19973062.3553415/150‑1 (MQ=60)
                                              TAACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGACCATTCAAAATGTAAGATTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGG                                                                                                       >  SRR19973062.873115/1‑150 (MQ=60)
                                               TACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGC                                                                                                      <  SRR19973062.388616/150‑1 (MQ=60)
                                                ACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCT                                                                                                     >  SRR19973062.2893190/1‑150 (MQ=60)
                                                 CTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTC                                                                                                    <  SRR19973062.166000/150‑1 (MQ=60)
                                                   GGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCAT                                                                                                  >  SRR19973062.290843/1‑150 (MQ=60)
                                                    GCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATA                                                                                                 >  SRR19973062.3778556/1‑150 (MQ=60)
                                                     CCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAG                                                                                                <  SRR19973062.1940810/150‑1 (MQ=60)
                                                     CCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAG                                                                                                <  SRR19973062.1940675/150‑1 (MQ=60)
                                                     CCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAG                                                                                                <  SRR19973062.700355/150‑1 (MQ=60)
                                                      CGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGG                                                                                               <  SRR19973062.3508623/150‑1 (MQ=60)
                                                      CGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGG                                                                                               <  SRR19973062.872995/150‑1 (MQ=60)
                                                      CGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGG                                                                                               >  SRR19973062.2371508/1‑150 (MQ=60)
                                                      CGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGG                                                                                               <  SRR19973062.1325499/150‑1 (MQ=60)
                                                        CAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGG                                                                                             >  SRR19973062.1680392/1‑150 (MQ=60)
                                                        CAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGG                                                                                             >  SRR19973062.1763038/1‑150 (MQ=60)
                                                        CAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGG                                                                                             >  SRR19973062.2052878/1‑150 (MQ=60)
                                                          GGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTG                                                                                           <  SRR19973062.772925/150‑1 (MQ=60)
                                                          GGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTG                                                                                           <  SRR19973062.169209/150‑1 (MQ=60)
                                                          GGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTG                                                                                           <  SRR19973062.169223/150‑1 (MQ=60)
                                                           GTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGA                                                                                          <  SRR19973062.1339108/150‑1 (MQ=60)
                                                             GTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATA                                                                                        >  SRR19973062.3807679/1‑150 (MQ=60)
                                                             GTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATA                                                                                        <  SRR19973062.412029/150‑1 (MQ=60)
                                                              TCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAG                                                                                       <  SRR19973062.416617/150‑1 (MQ=60)
                                                                ACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTA                                                                                     <  SRR19973062.3106558/150‑1 (MQ=60)
                                                                ACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTA                                                                                     <  SRR19973062.3106521/150‑1 (MQ=60)
                                                                  CGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATT                                                                                   >  SRR19973062.4251539/1‑150 (MQ=60)
                                                                   GTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTT                                                                                  <  SRR19973062.1167538/150‑1 (MQ=60)
                                                                    TAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTG                                                                                 <  SRR19973062.2104215/150‑1 (MQ=60)
                                                                      ACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTA                                                                               >  SRR19973062.3854894/1‑150 (MQ=60)
                                                                      ACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTA                                                                               <  SRR19973062.2226224/150‑1 (MQ=60)
                                                                       CTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTAC                                                                              >  SRR19973062.3966463/1‑150 (MQ=60)
                                                                       CTGAATAACCACTGGAGTAACTGGTCTGATTATTTGACAAAGTGCTGTTGTTTTTATTGGTCCATTCAAATTGTAAGTTTAATTGTATGGTATCGTTATATAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTAC                                                                              <  SRR19973062.3006970/150‑1 (MQ=60)
                                                                        TGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATTTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTACAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACT                                                                             <  SRR19973062.1322858/150‑1 (MQ=60)
                                                                        TGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACT                                                                             >  SRR19973062.35728/1‑150 (MQ=60)
                                                                        TGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACT                                                                             >  SRR19973062.5160521/1‑150 (MQ=60)
                                                                        TGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACT                                                                             >  SRR19973062.2505575/1‑150 (MQ=60)
                                                                          AATAACCACTTGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTACATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTA                                                                           <  SRR19973062.1417194/150‑1 (MQ=60)
                                                                          AATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTA                                                                           <  SRR19973062.1416884/150‑1 (MQ=60)
                                                                            TAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACG                                                                         <  SRR19973062.1885202/150‑1 (MQ=60)
                                                                            TAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACG                                                                         >  SRR19973062.2340141/1‑150 (MQ=60)
                                                                               CCACTGGTTAAACTGTTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAT                                                                      <  SRR19973062.2010545/150‑1 (MQ=60)
                                                                               CCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAT                                                                      >  SRR19973062.638039/1‑150 (MQ=60)
                                                                               CCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCAT                                                                      <  SRR19973062.2010518/150‑1 (MQ=60)
                                                                                CACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATG                                                                     <  SRR19973062.291026/150‑1 (MQ=60)
                                                                                 ACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGA                                                                    <  SRR19973062.4574818/150‑1 (MQ=60)
                                                                                 ACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGA                                                                    <  SRR19973062.1839152/150‑1 (MQ=60)
                                                                                  CTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGAC                                                                   >  SRR19973062.922903/1‑150 (MQ=60)
                                                                                    GGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGA                                                                 >  SRR19973062.2368754/1‑150 (MQ=60)
                                                                                    GGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGA                                                                 >  SRR19973062.2368886/1‑150 (MQ=60)
                                                                                       GAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATC                                                              >  SRR19973062.3728949/1‑150 (MQ=60)
                                                                                         AACTGGTCTGATTTTTTGACAAAGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAT                                                            <  SRR19973062.2521518/150‑1 (MQ=60)
                                                                                         AACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAT                                                            >  SRR19973062.3522265/1‑150 (MQ=60)
                                                                                         AACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCAT                                                            <  SRR19973062.2521418/150‑1 (MQ=60)
                                                                                          ACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATT                                                           >  SRR19973062.45261/1‑150 (MQ=60)
                                                                                          ACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATT                                                           >  SRR19973062.485242/1‑150 (MQ=60)
                                                                                          ACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATT                                                           <  SRR19973062.649270/150‑1 (MQ=60)
                                                                                           CTGGTCTGATTTTTTGACATCGTGATGTTGTTTTTATTGGTCCATTCAAATTGTTAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTA                                                          <  SRR19973062.3182569/150‑1 (MQ=60)
                                                                                           CTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTA                                                          <  SRR19973062.1609600/150‑1 (MQ=60)
                                                                                           CTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTA                                                          <  SRR19973062.2945592/150‑1 (MQ=60)
                                                                                           CTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTA                                                          <  SRR19973062.1609252/150‑1 (MQ=60)
                                                                                            TGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAA                                                         >  SRR19973062.4428773/1‑150 (MQ=60)
                                                                                            TGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTAAGCATGACGAATCATTAA                                                         >  SRR19973062.505786/1‑150 (MQ=60)
                                                                                             GGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAA                                                        >  SRR19973062.1408304/1‑150 (MQ=60)
                                                                                              GTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAA                                                       <  SRR19973062.1215765/150‑1 (MQ=60)
                                                                                                CTGATTATTTGACAACTTGATGTTGTTTTTATTGGTCCATTCAAAATGTATGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATT                                                     <  SRR19973062.499607/150‑1 (MQ=60)
                                                                                                CTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATT                                                     <  SRR19973062.1018176/150‑1 (MQ=60)
                                                                                                CTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATT                                                     <  SRR19973062.3436415/150‑1 (MQ=60)
                                                                                                   ATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATG                                                  <  SRR19973062.162968/150‑1 (MQ=60)
                                                                                                      ATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTC                                               <  SRR19973062.3512979/150‑1 (MQ=60)
                                                                                                      ATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTC                                               <  SRR19973062.770307/150‑1 (MQ=60)
                                                                                                          GACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATAT                                           <  SRR19973062.4384701/150‑1 (MQ=60)
                                                                                                           ACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATG                                          <  SRR19973062.4584213/150‑1 (MQ=60)
                                                                                                            CAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGT                                         >  SRR19973062.873433/1‑150 (MQ=60)
                                                                                                            CAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGT                                         >  SRR19973062.141785/1‑150 (MQ=60)
                                                                                                            CAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGT                                         >  SRR19973062.1934751/1‑150 (MQ=60)
                                                                                                             AACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTC                                        >  SRR19973062.779446/1‑150 (MQ=60)
                                                                                                             AACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAAAATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTC                                        >  SRR19973062.5327419/1‑150 (MQ=60)
                                                                                                              ACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCG                                       <  SRR19973062.4927691/150‑1 (MQ=60)
                                                                                                                GTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCC                                     <  SRR19973062.1984593/150‑1 (MQ=60)
                                                                                                                 TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                    >  SRR19973062.2531951/1‑150 (MQ=60)
                                                                                                                 TGATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCA                                    <  SRR19973062.1472498/150‑1 (MQ=60)
                                                                                                                  GATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAT                                   <  SRR19973062.833139/150‑1 (MQ=60)
                                                                                                                   ATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATT                                  <  SRR19973062.2019450/150‑1 (MQ=60)
                                                                                                                   ATGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATT                                  <  SRR19973062.1619832/150‑1 (MQ=60)
                                                                                                                    TGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTT                                 <  SRR19973062.5226875/150‑1 (MQ=60)
                                                                                                                    TGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTT                                 <  SRR19973062.2697012/150‑1 (MQ=60)
                                                                                                                    TGTTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTT                                 <  SRR19973062.4863661/150‑1 (MQ=60)
                                                                                                                    TGTTGTTTTTAGTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTT                                 <  SRR19973062.5226746/150‑1 (MQ=60)
                                                                                                                      TTGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGT                               <  SRR19973062.2498357/150‑1 (MQ=60)
                                                                                                                       TGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTG                              <  SRR19973062.1937950/150‑1 (MQ=60)
                                                                                                                       TGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTG                              <  SRR19973062.1937893/150‑1 (MQ=60)
                                                                                                                       TGTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTG                              >  SRR19973062.31037/1‑150 (MQ=60)
                                                                                                                        GTTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGT                             >  SRR19973062.1957878/1‑150 (MQ=60)
                                                                                                                         TTTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTT                            <  SRR19973062.3258418/150‑1 (MQ=60)
                                                                                                                          TTTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTT                           <  SRR19973062.4181380/150‑1 (MQ=60)
                                                                                                                           TTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTG                          >  SRR19973062.1039079/1‑150 (MQ=60)
                                                                                                                           TTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTG                          >  SRR19973062.1039167/1‑150 (MQ=60)
                                                                                                                           TTTATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTG                          >  SRR19973062.3949670/1‑150 (MQ=60)
                                                                                                                             TATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAG                        <  SRR19973062.276745/150‑1 (MQ=60)
                                                                                                                             TATTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAG                        >  SRR19973062.2383305/1‑150 (MQ=60)
                                                                                                                               TTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTA                      <  SRR19973062.2147311/150‑1 (MQ=60)
                                                                                                                               TTGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTA                      <  SRR19973062.4012122/150‑1 (MQ=60)
                                                                                                                                TGGTCCATTCAAAATGTAATTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAA                     <  SRR19973062.1800396/150‑1 (MQ=60)
                                                                                                                                TGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAA                     >  SRR19973062.4495879/1‑150 (MQ=60)
                                                                                                                                TGGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAA                     >  SRR19973062.4496060/1‑150 (MQ=60)
                                                                                                                                 GGTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAG                    >  SRR19973062.3912733/1‑150 (MQ=60)
                                                                                                                                  GTCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCAGTTGTGTTTGAGTAAGT                   >  SRR19973062.2919008/1‑150 (MQ=60)
                                                                                                                                   TCCATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTG                  <  SRR19973062.2571847/150‑1 (MQ=60)
                                                                                                                                      ATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAA               >  SRR19973062.1008880/1‑150 (MQ=60)
                                                                                                                                      ATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAA               >  SRR19973062.5160739/1‑150 (MQ=60)
                                                                                                                                      ATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAA               >  SRR19973062.1008872/1‑150 (MQ=60)
                                                                                                                                      ATTCAAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAA               >  SRR19973062.3009592/1‑150 (MQ=60)
                                                                                                                                          AAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATC           <  SRR19973062.3848287/150‑1 (MQ=60)
                                                                                                                                          AAAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATC           >  SRR19973062.2391388/1‑150 (MQ=60)
                                                                                                                                           AAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCC          >  SRR19973062.4456451/1‑150 (MQ=60)
                                                                                                                                           AAATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCC          >  SRR19973062.3933924/1‑150 (MQ=60)
                                                                                                                                             ATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCC        <  SRR19973062.4960956/150‑1 (MQ=60)
                                                                                                                                             ATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCC        >  SRR19973062.2902387/1‑150 (MQ=60)
                                                                                                                                             ATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCC        >  SRR19973062.2542500/1‑150 (MQ=60)
                                                                                                                                             ATGTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCC        >  SRR19973062.2542456/1‑150 (MQ=60)
                                                                                                                                               GTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCT      <  SRR19973062.564453/150‑1 (MQ=60)
                                                                                                                                               GTAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCT      >  SRR19973062.1314533/1‑150 (MQ=60)
                                                                                                                                                TAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG     >  SRR19973062.965434/1‑150 (MQ=60)
                                                                                                                                                TAAGTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTG     <  SRR19973062.506244/150‑1 (MQ=60)
                                                                                                                                                   GTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  <  SRR19973062.3426503/150‑1 (MQ=60)
                                                                                                                                                   GTTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  <  SRR19973062.3426657/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
AATCATCACTGAACCGCCGCCCCCCGCAGAAACATTCACTTTTGCATTACTGGCCGCAGGTGTCACCGTAACTGAATAACCACTGGTGAAACTGGTCTGATTATTTGACAACGTGATGTTGTTTTTATTGGTCCATTCAAAATGTAAATTTAATTTTATGGTATCGTTATAAAGTCCAACAATAATATCCGACGGGCTCATAGGGGTGATAGTATTTGTACTTACGCATGACGAATCATTAAAATTATGCTCATATGTCGCCATTTGTGTTTGAGTAAGTGCAAAATCCCCCTGAAC  >  CP009273/2181498‑2181794

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑