Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,481,488 A→T 29.5% intergenic (+42/+14) evgS → / ← yfdE hybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,481,4880AT29.5% 563.2 / 310.6 362intergenic (+42/+14)evgS/yfdEhybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (144/110);  new base T (56/51);  total (201/161)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 366 total aligned reads displayed.

GTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAAC  >  CP009273/2481339‑2481637
                                                                                                                                                     |                                                                                                                                                     
gTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCa                                                                                                                                                       >  2:3313825/1‑150 (MQ=255)
    cAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGcac                                                                                                                                                   >  2:5312329/1‑150 (MQ=255)
           cAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTAc                                                                                                                                            <  1:2407782/150‑1 (MQ=255)
              tAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACAtt                                                                                                                                         >  2:2723398/1‑150 (MQ=255)
                aGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTa                                                                                                                                       >  1:2778888/1‑150 (MQ=255)
                 tccTTAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTat                                                                                                                                      >  2:3834861/2‑150 (MQ=255)
                 gCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAATAACACTTTGCATAGCTGGACCAGTAGTTTGCTGTTTTCTGTCATAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTat                                                                                                                                      <  1:1455514/150‑1 (MQ=255)
                 gCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTat                                                                                                                                      <  1:654745/150‑1 (MQ=255)
                 gCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAACAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTat                                                                                                                                      <  1:399640/150‑1 (MQ=255)
                  ccTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTatg                                                                                                                                     >  2:846193/1‑150 (MQ=255)
                  ccTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatg                                                                                                                                     <  2:1529990/150‑1 (MQ=255)
                   cTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatga                                                                                                                                    >  2:3451776/1‑150 (MQ=255)
                     gAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgatg                                                                                                                                  <  2:1273260/150‑1 (MQ=255)
                       aaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATgag                                                                                                                                <  1:3630866/150‑1 (MQ=255)
                        aTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGCGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATgaga                                                                                                                               <  1:1606702/150‑1 (MQ=255)
                         ttcttcAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGaa                                                                                                                              <  2:3748346/150‑1 (MQ=255)
                           cttcAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACAGTTATGATGAGAACt                                                                                                                            >  1:1711646/1‑150 (MQ=255)
                              cAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCt                                                                                                                         <  1:3340960/150‑1 (MQ=255)
                               aGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTg                                                                                                                        <  2:1174883/150‑1 (MQ=255)
                                  tGCTGAACTCCGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCg                                                                                                                     >  1:335023/1‑150 (MQ=255)
                                        aCTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTg                                                                                                               >  1:592855/1‑150 (MQ=255)
                                         ctctGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGt                                                                                                              >  2:830327/1‑150 (MQ=255)
                                                aaaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCAt                                                                                                       >  2:2337233/1‑150 (MQ=255)
                                                      cacaTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGt                                                                                                 <  1:2106750/150‑1 (MQ=255)
                                                        caTTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCg                                                                                               >  2:3222034/1‑150 (MQ=255)
                                                              agagCTGGACCAGGAGATTGCTGTTTACTGTCAGAAAAAAGACTAAATAGCGTCTCCCACAAAGTTCAAATGTGCGAGCTAGTTACCAGCACATCTTACATTTCTCATGAGAACTCATGGCGAATATGTTCCCCATGCTGATCGAGCGtt                                                                                         >  2:3015924/1‑150 (MQ=255)
                                                                agCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                                       >  1:2487436/1‑150 (MQ=255)
                                                                 gCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGcc                                                                                      <  1:5005154/150‑1 (MQ=255)
                                                                        cAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCg                                                                               >  1:4092972/1‑150 (MQ=255)
                                                                        cAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCg                                                                               >  1:157991/1‑150 (MQ=255)
                                                                         aGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCgg                                                                              >  1:1343070/1‑150 (MQ=255)
                                                                          ggAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGc                                                                             >  1:3893735/1‑150 (MQ=255)
                                                                            agaTTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCAt                                                                           <  1:2374007/150‑1 (MQ=255)
                                                                             gaTTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATa                                                                          >  1:5282624/1‑150 (MQ=255)
                                                                                  cTGTTTTCTGTCAGAAAAATGACTAAATCGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATg                                                                     <  1:973247/150‑1 (MQ=255)
                                                                                  cTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATg                                                                     >  1:1741549/1‑150 (MQ=255)
                                                                                  cTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATg                                                                     >  1:3121112/1‑150 (MQ=255)
                                                                                   tGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTCTGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                    <  1:2630322/150‑1 (MQ=255)
                                                                                    gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCg                                                                   >  1:1840911/1‑150 (MQ=255)
                                                                                    gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCg                                                                   >  1:3280204/1‑150 (MQ=255)
                                                                                      tttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACTATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCggg                                                                 <  1:4794905/150‑1 (MQ=255)
                                                                                      tttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCggg                                                                 >  2:3396989/1‑150 (MQ=255)
                                                                                         cTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                              <  2:4802112/150‑1 (MQ=255)
                                                                                         cTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                              >  1:3457576/1‑150 (MQ=255)
                                                                                         cTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                              <  2:5250490/150‑1 (MQ=255)
                                                                                           gTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCcgc                                                            <  1:3692544/150‑1 (MQ=255)
                                                                                              aGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCa                                                         >  1:2707273/1‑150 (MQ=255)
                                                                                                 aaaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGcc                                                      <  1:113546/150‑1 (MQ=255)
                                                                                                 aaaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGcc                                                      >  1:3070958/1‑150 (MQ=255)
                                                                                                  aaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCg                                                     <  2:639534/150‑1 (MQ=255)
                                                                                                   aaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGACCTCCTGGCGAAGTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGc                                                    >  2:2963965/1‑150 (MQ=255)
                                                                                                   aaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGc                                                    >  2:1843265/1‑150 (MQ=255)
                                                                                                     tGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTg                                                  >  2:1559009/1‑150 (MQ=255)
                                                                                                       aCTAAATCGCGGCTCCCACAATGTTCAACTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAt                                                <  1:5111878/150‑1 (MQ=255)
                                                                                                        cTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCCGCCGCTGAtt                                               >  2:4758127/1‑150 (MQ=255)
                                                                                                           aaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttttt                                            >  2:4678323/1‑150 (MQ=255)
                                                                                                             tAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                          >  2:414748/1‑150 (MQ=255)
                                                                                                              aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATc                                         >  2:4424664/1‑150 (MQ=255)
                                                                                                              aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTAGGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATc                                         <  2:2143306/150‑1 (MQ=255)
                                                                                                                 ggCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGTTTTTTATCGGa                                      >  1:2277508/1‑150 (MQ=255)
                                                                                                                 ggCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGa                                      >  2:5134732/1‑150 (MQ=255)
                                                                                                                  gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAt                                     >  2:2612278/1‑150 (MQ=255)
                                                                                                                   cTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAtt                                    <  1:2858276/150‑1 (MQ=255)
                                                                                                                   cTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAtt                                    <  2:2356519/150‑1 (MQ=255)
                                                                                                                    tCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTa                                   >  2:1486056/1‑150 (MQ=255)
                                                                                                                    tCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTa                                   >  2:3964707/1‑150 (MQ=255)
                                                                                                                     cccACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAc                                  <  1:4525230/150‑1 (MQ=255)
                                                                                                                          aaTGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGc                             >  1:4596623/1‑150 (MQ=255)
                                                                                                                            tGTTCAACTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcat                           <  2:2816285/150‑1 (MQ=255)
                                                                                                                            tGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcat                           <  2:205321/150‑1 (MQ=255)
                                                                                                                              ttCAAATGTGGGAGCTAATTACCAGCACATCTTACTTTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatca                         <  1:4315415/150‑1 (MQ=255)
                                                                                                                               tCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatcat                        >  2:1371721/1‑150 (MQ=255)
                                                                                                                               tCAAATGTGGGAGCTATTTACCAGCACATCTTACATGTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatcat                        <  2:1661916/150‑1 (MQ=255)
                                                                                                                                 aaaTGTGGGAGCTATTTACCAGCACATCTTACCTTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                      <  2:5040955/150‑1 (MQ=255)
                                                                                                                                 aaaTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                      >  1:1088515/1‑150 (MQ=255)
                                                                                                                                 aaaTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                      >  1:3777963/1‑150 (MQ=255)
                                                                                                                                  aaTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattat                     <  2:2471546/150‑1 (MQ=255)
                                                                                                                                   aTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                    <  2:3500151/150‑1 (MQ=255)
                                                                                                                                   aTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                    >  2:2128046/1‑150 (MQ=255)
                                                                                                                                   aTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGTGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                    >  1:4734221/1‑150 (MQ=255)
                                                                                                                                   aTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCattatt                    <  2:1073834/150‑1 (MQ=255)
                                                                                                                                    tgtgGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTc                   <  2:1243618/150‑1 (MQ=255)
                                                                                                                                     gtgGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTcc                  >  1:859856/1‑150 (MQ=255)
                                                                                                                                       gggAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTcccc                <  2:3906576/150‑1 (MQ=255)
                                                                                                                                       gggAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTcccc                >  1:1210439/1‑150 (MQ=255)
                                                                                                                                        ggAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTccccc               <  1:5312336/150‑1 (MQ=255)
                                                                                                                                        ggAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTccccc               <  1:2723398/150‑1 (MQ=255)
                                                                                                                                        ggAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTccccc               >  1:2165337/1‑150 (MQ=255)
                                                                                                                                         gAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCg              >  2:4942509/1‑150 (MQ=255)
                                                                                                                                         gAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCg              <  2:4535635/150‑1 (MQ=255)
                                                                                                                                          aGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCgg             <  1:297542/150‑1 (MQ=255)
                                                                                                                                            cTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGcg           >  2:911316/1‑149 (MQ=255)
                                                                                                                                            cTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCt           >  1:1025535/1‑150 (MQ=255)
                                                                                                                                             tATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGACTTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTGTTATCGGATTACCCGGCATCATTATTCCCCCGGCtt          >  2:2651053/1‑150 (MQ=255)
                                                                                                                                             tATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCtt          <  2:2230399/150‑1 (MQ=255)
                                                                                                                                             tATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCtt          <  2:540066/150‑1 (MQ=255)
                                                                                                                                             tATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAAAATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCtt          >  2:4059326/1‑150 (MQ=255)
                                                                                                                                                ttACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaa       >  2:1914896/1‑150 (MQ=255)
                                                                                                                                                 tACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaat      >  2:3626498/1‑150 (MQ=255)
                                                                                                                                                    cAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaatcaa   <  1:5074680/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GTTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCAAC  >  CP009273/2481339‑2481637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A