Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,481,488 A→T 16.0% intergenic (+42/+14) evgS → / ← yfdE hybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,481,4880AT16.0% 745.4 / 120.2 281intergenic (+42/+14)evgS/yfdEhybrid sensory histidine kinase in two‑component regulatory system with EvgA/acetyl‑CoA:oxalate CoA‑transferase
Reads supporting (aligned to +/- strand):  ref base A (130/106);  new base T (25/20);  total (155/126)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

Only 100 of 284 total aligned reads displayed.

TTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCA  >  CP009273/2481340‑2481635
                                                                                                                                                    |                                                                                                                                                   
   cAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGca                                                                                                                                                  <  2:2261376/149‑1 (MQ=255)
      aTGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAAAGTGGGAGCTATTTACCAGCACAt                                                                                                                                               >  1:1016297/1‑149 (MQ=255)
       tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATc                                                                                                                                              >  2:2398546/1‑149 (MQ=255)
       tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATc                                                                                                                                              >  1:4088691/1‑149 (MQ=255)
       tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATc                                                                                                                                              >  1:2052347/1‑149 (MQ=255)
       tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATc                                                                                                                                              >  1:3505813/1‑149 (MQ=255)
       tGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATc                                                                                                                                              >  1:2370321/1‑149 (MQ=255)
         aCAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCtt                                                                                                                                            <  1:3599300/149‑1 (MQ=255)
            gTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACa                                                                                                                                         >  1:2969329/1‑149 (MQ=255)
            gTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACa                                                                                                                                         <  2:3515059/149‑1 (MQ=255)
            gTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACa                                                                                                                                         <  1:552387/149‑1 (MQ=255)
             tAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACAt                                                                                                                                        <  1:1819486/149‑1 (MQ=255)
               aGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACAttt                                                                                                                                      <  1:842056/149‑1 (MQ=255)
               aGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACAttt                                                                                                                                      >  2:3811061/1‑149 (MQ=255)
                 ccTTAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTat                                                                                                                                    <  1:78066/149‑1 (MQ=255)
                   tGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGGTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatga                                                                                                                                  <  2:171687/149‑1 (MQ=255)
                    gAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgat                                                                                                                                 <  1:2148349/149‑1 (MQ=255)
                    gAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgat                                                                                                                                 >  2:1171047/1‑149 (MQ=255)
                     aaaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTatgatg                                                                                                                                >  2:2884071/1‑149 (MQ=255)
                      aaTTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTAtgatga                                                                                                                               >  1:2501669/1‑149 (MQ=255)
                         tcttcAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGaa                                                                                                                            >  2:3346305/1‑149 (MQ=255)
                              atttGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCt                                                                                                                       >  2:3853860/3‑149 (MQ=255)
                              aGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCt                                                                                                                       <  1:3199444/149‑1 (MQ=255)
                                   cTGAAATATGTAAAAGAACACATTCCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGa                                                                                                                  <  1:2109195/149‑1 (MQ=255)
                                    tGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGaa                                                                                                                 <  2:1377527/149‑1 (MQ=255)
                                     gAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGTCGAAt                                                                                                                >  1:228449/1‑149 (MQ=255)
                                          ctGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGtt                                                                                                           >  1:3732664/1‑149 (MQ=255)
                                           tGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTc                                                                                                          <  2:264944/149‑1 (MQ=255)
                                               aaaGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCa                                                                                                      <  2:2501669/149‑1 (MQ=255)
                                                 aGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATg                                                                                                    >  1:1209647/1‑149 (MQ=255)
                                                  gAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGc                                                                                                   >  2:3574546/1‑149 (MQ=255)
                                                   aaCACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCt                                                                                                  <  1:3267922/149‑1 (MQ=255)
                                                   aaCACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCt                                                                                                  >  2:3673357/1‑149 (MQ=255)
                                                         ttGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGACTTTGTTCCCCATGCTGGTCGa                                                                                            >  1:2932573/1‑149 (MQ=255)
                                                           gCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGc                                                                                          <  1:62490/149‑1 (MQ=255)
                                                                gCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                                     >  2:2089582/1‑149 (MQ=255)
                                                                gCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGc                                                                                     <  1:2564426/149‑1 (MQ=255)
                                                                  tGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCg                                                                                   >  2:1965930/1‑149 (MQ=255)
                                                                      ccAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTcc                                                                               >  1:655421/1‑149 (MQ=255)
                                                                         ggAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAAGTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCgg                                                                            >  2:532673/1‑149 (MQ=255)
                                                                              ttGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATaa                                                                       >  1:3361463/1‑149 (MQ=255)
                                                                              ttGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATaa                                                                       >  1:1976335/1‑149 (MQ=255)
                                                                              ttGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATaa                                                                       <  2:326606/149‑1 (MQ=255)
                                                                                gCTGTTTTCTGTCATAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACa                                                                     >  1:2229508/1‑149 (MQ=255)
                                                                                   gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                  >  1:925491/1‑149 (MQ=255)
                                                                                   gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                  <  1:2290709/149‑1 (MQ=255)
                                                                                   gTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGc                                                                  <  1:4066807/149‑1 (MQ=255)
                                                                                     tttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCgg                                                                <  1:2754352/149‑1 (MQ=255)
                                                                                      ttCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCggg                                                               <  1:2490971/149‑1 (MQ=255)
                                                                                         tGTCAGAAAAATGACTAACTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTcc                                                            <  2:1465991/149‑1 (MQ=255)
                                                                                          gTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCcg                                                           >  2:2858269/1‑149 (MQ=255)
                                                                                            cAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCcgcg                                                         >  1:3157103/1‑149 (MQ=255)
                                                                                             aGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCgcgc                                                        >  2:1380578/1‑149 (MQ=255)
                                                                                                aaaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGc                                                     >  1:855703/1‑149 (MQ=255)
                                                                                                aaaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGc                                                     >  2:3559081/1‑149 (MQ=255)
                                                                                                 aaaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAAGTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGcc                                                    >  2:3050453/1‑149 (MQ=255)
                                                                                                  aaTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCg                                                   >  1:137134/1‑149 (MQ=255)
                                                                                                   aTGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGc                                                  <  2:4088691/149‑1 (MQ=255)
                                                                                                    tGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCt                                                 <  1:3098251/149‑1 (MQ=255)
                                                                                                     gACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTg                                                >  2:1120839/1‑149 (MQ=255)
                                                                                                      aCTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGa                                               <  2:1068627/149‑1 (MQ=255)
                                                                                                      aCTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGa                                               <  2:1381601/149‑1 (MQ=255)
                                                                                                      aCTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGa                                               <  1:2605536/149‑1 (MQ=255)
                                                                                                       cTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAt                                              >  2:454277/1‑149 (MQ=255)
                                                                                                       cTAAAGAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAt                                              <  1:17617/149‑1 (MQ=255)
                                                                                                        tAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAtt                                             >  1:3559128/1‑149 (MQ=255)
                                                                                                         aaaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttt                                            >  2:2770930/1‑149 (MQ=255)
                                                                                                         aaaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttt                                            <  1:1317197/149‑1 (MQ=255)
                                                                                                          aaTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAtttt                                           >  1:2679743/1‑149 (MQ=255)
                                                                                                           aTAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGAttttt                                          <  2:1828242/149‑1 (MQ=255)
                                                                                                             aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                        <  1:1868178/149‑1 (MQ=255)
                                                                                                             aGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTAt                                        <  1:3673357/149‑1 (MQ=255)
                                                                                                              gCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATc                                       <  2:2969513/149‑1 (MQ=255)
                                                                                                               cGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCg                                      <  2:1858088/149‑1 (MQ=255)
                                                                                                                 gCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGa                                    <  1:3005816/149‑1 (MQ=255)
                                                                                                                  cTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAt                                   <  1:1082486/149‑1 (MQ=255)
                                                                                                                   tCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGAtt                                  <  1:3958935/149‑1 (MQ=255)
                                                                                                                      cacaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAcc                               >  2:3995553/1‑149 (MQ=255)
                                                                                                                       acaATGTTCAACTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAccc                              <  2:2499826/149‑1 (MQ=255)
                                                                                                                       acaATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTAccc                              >  2:2505485/1‑149 (MQ=255)
                                                                                                                          aTGTTCAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGc                           <  1:2664565/149‑1 (MQ=255)
                                                                                                                           tGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACACCTGGCGAATTTGTTCACCATGCTGGTCGAGAGTAGCAGCTCCCGGCATAACATCAGGCTCCGCGCAGCCGCTGATTGTTATCGGATTAACCGGca                          >  2:2181746/1‑149 (MQ=255)
                                                                                                                              actaaTGTGGGAGCTAATTACCAGCTCATATTTCATTTATGATGAGAACTCCTGTAGAATTTGTTCCCCATGCTGGTCGAGCGTTGCAGAGCCAGTAATAACATGAGGGTCCGCTCAGCCGCTTTTTTTTATCTTATTACCCGGcatca                       <  2:268148/146‑1 (MQ=255)
                                                                                                                               cAAATGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatcat                      <  1:1332194/149‑1 (MQ=255)
                                                                                                                               cAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatcat                      <  2:3732664/149‑1 (MQ=255)
                                                                                                                               cAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGcatcat                      <  1:3853860/149‑1 (MQ=255)
                                                                                                                                aaaTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatt                     >  1:399056/1‑149 (MQ=255)
                                                                                                                                 aaTGTGGGAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                    >  1:166925/1‑149 (MQ=255)
                                                                                                                                 aaTGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCatta                    >  2:790736/1‑149 (MQ=255)
                                                                                                                                      gggAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTccc               >  1:1594354/1‑149 (MQ=255)
                                                                                                                                       ggAGCTATTTACCTGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCCTCATTATTcccc              >  1:1524979/1‑149 (MQ=255)
                                                                                                                                       ggAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTcccc              <  1:3711055/149‑1 (MQ=255)
                                                                                                                                       ggAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTcccc              <  2:4066219/149‑1 (MQ=255)
                                                                                                                                           cTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGc          <  2:736843/149‑1 (MQ=255)
                                                                                                                                            tATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCt         >  2:2946814/1‑149 (MQ=255)
                                                                                                                                            tATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCt         >  2:319217/1‑149 (MQ=255)
                                                                                                                                             aTTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCtt        <  1:3900115/149‑1 (MQ=255)
                                                                                                                                              tttACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtc       >  1:2399275/1‑149 (MQ=255)
                                                                                                                                                tACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCACTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaa     >  1:2216193/1‑149 (MQ=255)
                                                                                                                                                  ccAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTtcaatc   >  2:1270570/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTTCAGATGACAGTAAGCCTGAAATTCTTCAGTTGCTGAACTCTGTAAAAGAACACATTGCAGAGCTGGACCAGGAGATTGCTGTTTTCTGTCAGAAAAATGACTAAATAGCGGCTCCCACAATGTTCAAATGTGGGAGCTATTTACCAGCACATCTTACATTTATGATGAGAACTCCTGGCGAATTTGTTCCCCATGCTGGTCGAGCGTTGCCGCTCCCGGCATAACATGCGGGTCCGCGCAGCCGCTGATTTTTATCGGATTACCCGGCATCATTATTCCCCCGGCTTCAATCA  >  CP009273/2481340‑2481635

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A