Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,010,667 G→T 30.4% D417Y (GAC→TAC)  ygfK → putative Fe‑S subunit oxidoreductase subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,010,6670GT30.4% 535.6 / 210.0 283D417Y (GAC→TAC) ygfKputative Fe‑S subunit oxidoreductase subunit
Reads supporting (aligned to +/- strand):  ref base G (99/98);  new base T (33/53);  total (132/151)
Fisher's exact test for biased strand distribution p-value = 7.08e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 287 total aligned reads displayed.

CCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATC  >  CP009273/3010518‑3010816
                                                                                                                                                     |                                                                                                                                                     
 cAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGa                                                                                                                                                      >  1:4608120/1‑150 (MQ=255)
  aGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTAc                                                                                                                                                     <  2:1790736/150‑1 (MQ=255)
  aGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGAc                                                                                                                                                     <  1:251871/150‑1 (MQ=255)
  aGTCAGCTGACGATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTAc                                                                                                                                                     <  2:2189035/150‑1 (MQ=255)
     cAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTc                                                                                                                                                  >  1:4711190/1‑150 (MQ=255)
      aGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCg                                                                                                                                                 >  1:1229189/1‑150 (MQ=255)
       gCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGa                                                                                                                                                <  1:1733305/150‑1 (MQ=255)
       gCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGa                                                                                                                                                <  2:2809331/150‑1 (MQ=255)
                 cgcgATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAAc                                                                                                                                      <  2:322617/150‑1 (MQ=255)
                    gATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGa                                                                                                                                   <  2:2648066/150‑1 (MQ=255)
                    gATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGa                                                                                                                                   >  1:3407299/1‑150 (MQ=255)
                    gATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGa                                                                                                                                   >  2:4930587/1‑150 (MQ=255)
                     atatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGAc                                                                                                                                  >  2:3709264/1‑150 (MQ=255)
                     atatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGAc                                                                                                                                  <  1:1515918/150‑1 (MQ=255)
                     atatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGAc                                                                                                                                  <  1:721321/150‑1 (MQ=255)
                     atatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGAc                                                                                                                                  <  2:1186212/150‑1 (MQ=255)
                      tatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACt                                                                                                                                 <  1:4552679/150‑1 (MQ=255)
                      tatTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACt                                                                                                                                 <  2:790375/150‑1 (MQ=255)
                       atTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTg                                                                                                                                <  1:4408353/150‑1 (MQ=255)
                        tttttGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTgg                                                                                                                               >  1:2055321/1‑150 (MQ=255)
                              aTACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGcagcag                                                                                                                         >  1:5315829/1‑150 (MQ=255)
                                aCAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGAt                                                                                                                       <  2:4427261/150‑1 (MQ=255)
                                aCAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGAt                                                                                                                       >  1:4427129/1‑150 (MQ=255)
                                aCAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGAt                                                                                                                       >  1:3099775/1‑150 (MQ=255)
                                 cAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATg                                                                                                                      <  1:1497720/150‑1 (MQ=255)
                                 cAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATg                                                                                                                      <  1:2577118/150‑1 (MQ=255)
                                  aGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGc                                                                                                                     >  1:1011569/1‑150 (MQ=255)
                                  aGGTATTCGCCATATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGc                                                                                                                     >  1:2735256/1‑150 (MQ=255)
                                    gTATTCGCCCTATTACTCTGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGATGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGt                                                                                                                   <  1:978721/150‑1 (MQ=255)
                                    gTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGt                                                                                                                   <  2:3957864/150‑1 (MQ=255)
                                    gTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGt                                                                                                                   >  2:1714727/1‑150 (MQ=255)
                                     tATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGtt                                                                                                                  <  1:4456369/150‑1 (MQ=255)
                                      aTTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTa                                                                                                                 <  2:51162/150‑1 (MQ=255)
                                        tCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAAc                                                                                                               <  1:1289438/150‑1 (MQ=255)
                                         cGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAAcc                                                                                                              <  1:3219606/150‑1 (MQ=255)
                                         cGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAAcc                                                                                                              <  2:4975888/150‑1 (MQ=255)
                                           cccTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCAt                                                                                                            <  1:4536245/150‑1 (MQ=255)
                                               aTTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGaa                                                                                                        >  2:4411689/1‑150 (MQ=255)
                                               aTTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGaa                                                                                                        >  2:4138157/1‑150 (MQ=255)
                                                 tACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATa                                                                                                      >  2:3613915/1‑150 (MQ=255)
                                                 tACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATa                                                                                                      <  1:4861260/150‑1 (MQ=255)
                                                   cTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATaca                                                                                                    <  2:2141991/150‑1 (MQ=255)
                                                      tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACAccc                                                                                                 <  1:998414/150‑1 (MQ=255)
                                                      tGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACAccc                                                                                                 >  2:5011541/1‑150 (MQ=255)
                                                       ggCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCa                                                                                                >  1:4814627/1‑150 (MQ=255)
                                                       ggCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCa                                                                                                <  1:1687939/150‑1 (MQ=255)
                                                       ggCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCa                                                                                                >  2:1150099/1‑150 (MQ=255)
                                                        gCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAg                                                                                               <  2:1341361/150‑1 (MQ=255)
                                                         cAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGa                                                                                              >  2:2828371/1‑150 (MQ=255)
                                                          aaCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGaa                                                                                             >  2:4869263/1‑150 (MQ=255)
                                                           aCCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCCGCAGATGCGTTAACCATGGAATACACCCAGaaa                                                                                            >  1:3126643/1‑150 (MQ=255)
                                                             cGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAaca                                                                                          >  2:1057850/1‑150 (MQ=255)
                                                              gACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAacac                                                                                         >  1:2210497/1‑150 (MQ=255)
                                                                  tgctgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGa                                                                                     <  2:4608120/150‑1 (MQ=255)
                                                                  tgctgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGa                                                                                     <  1:5241253/150‑1 (MQ=255)
                                                                  tgctgAAACCTGGAGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGAATTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGa                                                                                     <  1:293389/150‑1 (MQ=255)
                                                                   gctgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGaa                                                                                    <  2:4106317/150‑1 (MQ=255)
                                                                    ctgAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACTCTGGAAg                                                                                   >  1:1707919/1‑150 (MQ=255)
                                                                       aaaCCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCa                                                                                <  2:5329019/150‑1 (MQ=255)
                                                                           cTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaag                                                                            >  2:2435521/1‑150 (MQ=255)
                                                                            tGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCagaaga                                                                           <  1:2296680/150‑1 (MQ=255)
                                                                             ggcggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAagag                                                                          >  1:3797806/1‑150 (MQ=255)
                                                                             ggcggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAagag                                                                          <  2:4721160/150‑1 (MQ=255)
                                                                              gcggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGc                                                                         <  1:5013700/150‑1 (MQ=255)
                                                                               cggcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCg                                                                        >  2:2022320/1‑150 (MQ=255)
                                                                                 gcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTa                                                                      >  1:4970340/1‑150 (MQ=255)
                                                                                 gcTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTa                                                                      >  2:3335355/1‑150 (MQ=255)
                                                                                   tATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTAtt                                                                    >  1:2356425/1‑150 (MQ=255)
                                                                                    aTCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTg                                                                   <  1:3319330/150‑1 (MQ=255)
                                                                                       tGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAg                                                                >  2:1491556/1‑150 (MQ=255)
                                                                                       tGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAg                                                                <  2:4281274/150‑1 (MQ=255)
                                                                                       tGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAg                                                                >  2:15836/1‑150 (MQ=255)
                                                                                         cgcTTAAGTGCCAGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTg                                                              <  1:2476837/150‑1 (MQ=255)
                                                                                           cTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGc                                                            >  2:2625321/1‑150 (MQ=255)
                                                                                            ttAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCa                                                           <  1:1953097/150‑1 (MQ=255)
                                                                                             tAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCag                                                          <  1:3027784/150‑1 (MQ=255)
                                                                                                    cTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCt                                                   <  1:3491374/150‑1 (MQ=255)
                                                                                                     tGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTg                                                  >  1:1450987/1‑150 (MQ=255)
                                                                                                       cATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGcc                                                <  1:324040/150‑1 (MQ=255)
                                                                                                       cATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGcc                                                >  1:157473/1‑150 (MQ=255)
                                                                                                         tGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGc                                              >  2:3893692/1‑150 (MQ=255)
                                                                                                          gcgcGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCt                                             <  2:2367968/150‑1 (MQ=255)
                                                                                                           cgcgAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTg                                            <  1:1846396/150‑1 (MQ=255)
                                                                                                                  tGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACt                                     >  1:2987747/1‑150 (MQ=255)
                                                                                                                  tGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACt                                     <  1:5113923/150‑1 (MQ=255)
                                                                                                                  tGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACt                                     <  2:2519039/150‑1 (MQ=255)
                                                                                                                   ggAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTg                                    >  2:4611547/1‑150 (MQ=255)
                                                                                                                         cTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGt                              >  1:3922464/1‑150 (MQ=255)
                                                                                                                         cTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGt                              >  1:3933214/1‑150 (MQ=255)
                                                                                                                          tCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGtt                             >  1:3635695/1‑150 (MQ=255)
                                                                                                                               cGCCTGGGGACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGcccc                        <  2:4224569/150‑1 (MQ=255)
                                                                                                                                     gggACTTGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTgtgt                  <  2:922791/150‑1 (MQ=255)
                                                                                                                                     gggACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTgtgt                  >  1:2626325/1‑150 (MQ=255)
                                                                                                                                         cTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTAct              >  1:4081802/1‑150 (MQ=255)
                                                                                                                                           tGACCATGTTTACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgc            <  1:3709264/150‑1 (MQ=255)
                                                                                                                                           tGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgc            <  1:2823461/150‑1 (MQ=255)
                                                                                                                                              ccATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcctg         >  2:453149/1‑150 (MQ=255)
                                                                                                                                              ccATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTActgcctg         >  2:3130720/1‑150 (MQ=255)
                                                                                                                                                   ttGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTa    <  2:4552176/150‑1 (MQ=255)
                                                                                                                                                    tGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTAt   <  2:677709/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
CCAGTCAGCTGACTATCCGCGATATTTTTGATACAGGTATTCGCCCTATTACTATGGCAACCGACCTGCTGAAACCTGGCGGCTATCTGCGCTTAAGTGCCTGCATGCGCGAGCTGGAAGGCTCCGACGCCTGGGGACTTGACCATGTTGACGTCGAACGACTGAACAGACTGGCAGCAGATGCGTTAACCATGGAATACACCCAGAAACACTGGAAGCCAGAAGAGCGTATTGAAGTGGCAGAAGACCTGCCGCTGACCGACTGCTACGTTGCCCCCTGTGTTACTGCCTGCGCTATC  >  CP009273/3010518‑3010816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A