Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,493,210 C→T 23.2% intergenic (+236/‑59) yhfL → / → frlA small lipoprotein/putative fructoselysine transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,493,2100CT23.2% 783.0 / 221.9 358intergenic (+236/‑59)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
Reads supporting (aligned to +/- strand):  ref base C (169/106);  new base T (56/27);  total (225/133)
Fisher's exact test for biased strand distribution p-value = 3.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 365 total aligned reads displayed.

TGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTAT  >  CP009273/3493061‑3493359
                                                                                                                                                     |                                                                                                                                                     
   ccccGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtt                                                                                                                                                    <  2:2714885/150‑1 (MQ=255)
    cccgcccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTGGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTNATGTTACCttt                                                                                                                                                   <  1:2763458/150‑1 (MQ=255)
    cccgcccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCttt                                                                                                                                                   <  1:799173/150‑1 (MQ=255)
    cccgcccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCttt                                                                                                                                                   <  1:5126388/150‑1 (MQ=255)
     ccgcccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCtttt                                                                                                                                                  >  2:4622783/1‑150 (MQ=255)
       gcccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTaa                                                                                                                                                <  1:4351315/150‑1 (MQ=255)
        cccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTaaa                                                                                                                                               >  2:2029957/1‑150 (MQ=255)
         ccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAAt                                                                                                                                              <  1:3907365/150‑1 (MQ=255)
         ccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAAt                                                                                                                                              <  2:5166792/150‑1 (MQ=255)
         ccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAAt                                                                                                                                              <  2:5089156/150‑1 (MQ=255)
         ccgTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAAt                                                                                                                                              <  1:613505/150‑1 (MQ=255)
            tAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGAc                                                                                                                                           <  1:3789854/150‑1 (MQ=255)
            tAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGAc                                                                                                                                           >  1:4949334/1‑150 (MQ=255)
            tAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGAc                                                                                                                                           >  2:837895/1‑150 (MQ=255)
               aaaTATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGc                                                                                                                                        >  1:2922128/1‑150 (MQ=255)
                 atatTTTCACTTTAAATTCAATTTGATAACTACCTCCCTTAGCCACTGCGCGATCCTGTGCGAATTTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCTTGTTATCTTTTAAATGACTGCaa                                                                                                                                      <  2:1414934/150‑1 (MQ=255)
                 atatTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCaa                                                                                                                                      >  2:1699333/1‑150 (MQ=255)
                       tCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACtctc                                                                                                                                >  2:3331646/1‑150 (MQ=255)
                         aCTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTccc                                                                                                                              >  1:3499510/1‑150 (MQ=255)
                          cTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTcccc                                                                                                                             <  1:5100459/150‑1 (MQ=255)
                          cTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACTTTTTAAATGACTGCAAACTCTcccc                                                                                                                             >  1:1638217/1‑150 (MQ=255)
                           tttAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCt                                                                                                                            <  2:868879/150‑1 (MQ=255)
                               aaTTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACaa                                                                                                                        <  2:4014069/150‑1 (MQ=255)
                               aaTTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACaa                                                                                                                        >  1:4424196/1‑150 (MQ=255)
                                  tCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGt                                                                                                                     >  1:1464843/1‑150 (MQ=255)
                                   cAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTa                                                                                                                    >  2:131969/1‑150 (MQ=255)
                                    aaTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAt                                                                                                                   >  2:3522550/1‑150 (MQ=255)
                                    aaTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAt                                                                                                                   >  1:1969381/1‑150 (MQ=255)
                                     aTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAtt                                                                                                                  >  2:1072927/1‑150 (MQ=255)
                                     aTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAtt                                                                                                                  <  1:4054886/150‑1 (MQ=255)
                                     aTTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTAtt                                                                                                                  >  1:2223273/1‑150 (MQ=255)
                                          aTAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACa                                                                                                             >  1:3155497/1‑150 (MQ=255)
                                          aTAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACa                                                                                                             >  2:4998114/1‑150 (MQ=255)
                                            aaCTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAg                                                                                                           >  2:1546130/1‑150 (MQ=255)
                                            aaCTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAg                                                                                                           <  1:3401625/150‑1 (MQ=255)
                                               tACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGaaa                                                                                                        <  1:1181147/150‑1 (MQ=255)
                                               tACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGaaa                                                                                                        <  2:2817285/150‑1 (MQ=255)
                                                aCAGCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACAGTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGaaaa                                                                                                       <  2:1157414/150‑1 (MQ=255)
                                                      aTTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTAt                                                                                                 <  2:3416362/150‑1 (MQ=255)
                                                        tAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCg                                                                                               <  1:2317044/150‑1 (MQ=255)
                                                         aGCCAGAGCGCGATTCTGTGCGAAATTTTGTGATCTTCCTCCTCATTACATAACATCATATGTTGTTTTATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTCCAACGTATTGGACAAGAAAAAGGTATCGc                                                                                              <  2:2883074/150‑1 (MQ=255)
                                                            cacaGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTAt                                                                                           <  2:3236497/150‑1 (MQ=255)
                                                             acaGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATg                                                                                          >  2:190192/1‑150 (MQ=255)
                                                             acaGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATg                                                                                          >  2:4697953/1‑150 (MQ=255)
                                                               aGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATggg                                                                                        >  1:286508/1‑150 (MQ=255)
                                                                 cgcgATCCTGTGCGAAATTTTGTGATCTTCCTCAACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTAATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGaa                                                                                      <  1:452269/150‑1 (MQ=255)
                                                                  gcgATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAg                                                                                     >  1:3199436/1‑150 (MQ=255)
                                                                    gATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGcc                                                                                   <  2:449576/150‑1 (MQ=255)
                                                                     aTCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCa                                                                                  <  1:1010160/150‑1 (MQ=255)
                                                                      tCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAg                                                                                 <  1:530999/150‑1 (MQ=255)
                                                                      tCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAg                                                                                 <  2:3041447/150‑1 (MQ=255)
                                                                       ccTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAgg                                                                                >  2:5261217/1‑150 (MQ=255)
                                                                         tgtgCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGaa                                                                              >  1:5192568/1‑150 (MQ=255)
                                                                              gAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCa                                                                         <  1:3579462/150‑1 (MQ=255)
                                                                              gAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCa                                                                         <  1:4696760/150‑1 (MQ=255)
                                                                                aaTTTTGTGATCTTCCTCCACATTACATAACATCATGTGTTGTTATATTCCTCATGCATTGTCATGTTACCATTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAAc                                                                       <  1:5225670/150‑1 (MQ=255)
                                                                                     tgtgATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAg                                                                  >  2:1017612/1‑150 (MQ=255)
                                                                                        gATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATGGTCATGTTACCTTTTAAATGTCTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTc                                                               <  2:2392324/150‑1 (MQ=255)
                                                                                          tCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCgg                                                             <  2:374443/150‑1 (MQ=255)
                                                                                           cTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGa                                                            >  2:791779/1‑150 (MQ=255)
                                                                                           cTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGa                                                            >  1:887983/1‑150 (MQ=255)
                                                                                           cTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACATTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGa                                                            <  1:3523520/150‑1 (MQ=255)
                                                                                            ttCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGAt                                                           <  1:4896454/150‑1 (MQ=255)
                                                                                                 ccACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCACGCTCGGATTTTgg                                                      >  1:2385212/1‑150 (MQ=255)
                                                                                                  cacaTTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTggg                                                     >  1:3716221/1‑150 (MQ=255)
                                                                                                     aTTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCg                                                  >  1:5270061/1‑150 (MQ=255)
                                                                                                     aTTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCg                                                  >  2:1598730/1‑150 (MQ=255)
                                                                                                         cATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCt                                              >  1:4978112/1‑150 (MQ=255)
                                                                                                         cATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCt                                              >  2:2163533/1‑150 (MQ=255)
                                                                                                         cATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCt                                              <  1:644063/150‑1 (MQ=255)
                                                                                                          aTAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCtt                                             >  2:3271744/1‑150 (MQ=255)
                                                                                                           tAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTg                                            >  1:1848701/1‑150 (MQ=255)
                                                                                                              catcatATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACACCGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCaa                                         <  2:2273924/150‑1 (MQ=255)
                                                                                                                 catATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCg                                      >  2:1275765/1‑150 (MQ=255)
                                                                                                                  atatGTTGTTATATTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGc                                     >  2:4177729/1‑150 (MQ=255)
                                                                                                                  atatGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGc                                     >  2:3617037/1‑150 (MQ=255)
                                                                                                                    atGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCg                                   >  1:1339155/1‑150 (MQ=255)
                                                                                                                      gttgttATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTc                                 <  2:4737343/150‑1 (MQ=255)
                                                                                                                      gttgttATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTc                                 >  1:2572962/1‑150 (MQ=255)
                                                                                                                        tgttATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCgg                               >  1:2034834/1‑150 (MQ=255)
                                                                                                                         gttATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCggg                              >  2:4316629/1‑150 (MQ=255)
                                                                                                                          ttATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGa                             >  1:3427913/1‑150 (MQ=255)
                                                                                                                           tataTTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGAc                            >  2:3524771/1‑150 (MQ=255)
                                                                                                                             tatTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACaa                          >  2:4402298/1‑150 (MQ=255)
                                                                                                                              atTCATCATGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAAc                         <  2:5225421/150‑1 (MQ=255)
                                                                                                                               ttCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAAcc                        >  1:2825406/1‑150 (MQ=255)
                                                                                                                                tcatcaTGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCGATGGGACGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCg                       >  1:3003092/1‑150 (MQ=255)
                                                                                                                                 catcatGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGt                      >  1:1063244/1‑150 (MQ=255)
                                                                                                                                  atcatGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTc                     >  2:3845179/1‑150 (MQ=255)
                                                                                                                                  atcatGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTACCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTc                     <  2:2594226/150‑1 (MQ=255)
                                                                                                                                    catGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCgg                   >  2:685979/1‑150 (MQ=255)
                                                                                                                                     atGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGc                  >  2:4106367/1‑150 (MQ=255)
                                                                                                                                     atGCATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGc                  >  1:4786167/1‑150 (MQ=255)
                                                                                                                                        cATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTcc               >  1:1114586/1‑150 (MQ=255)
                                                                                                                                        cATTGTCATGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGACGGGACAACCGTCGGCTcc               >  1:859026/1‑150 (MQ=255)
                                                                                                                                        cATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTcc               >  1:2668958/1‑150 (MQ=255)
                                                                                                                                               aTGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTAttt        >  2:4566720/1‑150 (MQ=255)
                                                                                                                                                tGTTATCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGAGTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTAtttt       >  2:252839/1‑150 (MQ=255)
                                                                                                                                                     ccTTTTACATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTAt  <  2:1803978/150‑1 (MQ=255)
                                                                                                                                                     ccTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTAt  >  2:5068292/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TGCCCCCGCCCGTAAAAATATTTTCACTTTAAATTCAATTTGATAACTACATCCATTAGCCACAGCGCGATCCTGTGCGAAATTTTGTGATCTTCCTCCACATTACATAACATCATATGTTGTTATATTCATCATGCATTGTCATGTTACCTTTTAAATGACTGCAAACTCTCCCCTACAACGTATTGGACAAGAAAAAGGTATCGCTATGGGAAGCCAGGAACTCCAACGCAAGCTCGGATTTTGGGCCGTTCTTGCAATCGCCGTCGGGACAACCGTCGGCTCCGGTATTTTTGTAT  >  CP009273/3493061‑3493359

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A