Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 4,063,127 T→A 15.6% N212I (AAT→ATT)  yihR ← mutarotase superfamily protein, YihR family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092734,063,1270TA15.6% 687.9 / 102.8 287N212I (AAT→ATT) yihRmutarotase superfamily protein, YihR family
Reads supporting (aligned to +/- strand):  ref base T (107/134);  new base A (23/23);  total (130/157)
Fisher's exact test for biased strand distribution p-value = 5.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 290 total aligned reads displayed.

GCTATAAACCTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGC  >  CP009273/4062979‑4063276
                                                                                                                                                    |                                                                                                                                                     
gCTATAAACCTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAtt                                                                                                                                                      <  2:1496885/150‑1 (MQ=255)
      aaCCTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATc                                                                                                                                                <  1:1112029/150‑1 (MQ=255)
        ccTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCa                                                                                                                                              <  1:3399902/150‑1 (MQ=255)
         ctacACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAg                                                                                                                                             <  2:2054319/147‑1 (MQ=255)
         cTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGACAATTAAATCCAg                                                                                                                                             >  2:3385598/1‑150 (MQ=255)
         cTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAg                                                                                                                                             <  1:4204483/150‑1 (MQ=255)
          tGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGc                                                                                                                                            >  1:4376176/1‑150 (MQ=255)
            cACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCtc                                                                                                                                          >  1:3028220/1‑150 (MQ=255)
            cACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGGGAAATTTAAATCCAGCtc                                                                                                                                          >  1:3370399/1‑150 (MQ=255)
              cccAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCtcat                                                                                                                                        <  2:3838711/150‑1 (MQ=255)
                cAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCtcatca                                                                                                                                      <  2:2203174/150‑1 (MQ=255)
                 agagTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATACAGCTCATCaa                                                                                                                                     >  2:5099850/1‑150 (MQ=255)
                 agagTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTAGCCTGCGTGAAATTTTAATACAGCTCATCaa                                                                                                                                     >  2:5039975/1‑150 (MQ=255)
                  gagTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAAc                                                                                                                                    <  1:3833788/150‑1 (MQ=255)
                    gTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTAGCCTGCGTGAAATTTAAATACAGCTCAACAACgt                                                                                                                                  >  1:4663464/1‑150 (MQ=255)
                     ttGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACgtg                                                                                                                                 <  2:1673059/150‑1 (MQ=255)
                     ttGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACgtg                                                                                                                                 <  2:3644704/150‑1 (MQ=255)
                     ttGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACgtg                                                                                                                                 >  1:2233658/1‑150 (MQ=255)
                        aTCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACgtggtg                                                                                                                              >  1:612699/1‑150 (MQ=255)
                        aTCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACgtggtg                                                                                                                              <  2:4818768/150‑1 (MQ=255)
                           ggAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAg                                                                                                                           <  1:975516/150‑1 (MQ=255)
                            gAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGc                                                                                                                          >  2:4011918/1‑150 (MQ=255)
                            gAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGGGAAATTTAAATACAGCTCATCAACGTGGTGAAGc                                                                                                                          >  2:4597571/1‑150 (MQ=255)
                             aaCAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGAGTTTTGTCGCGGCTATTTTTTTAGCCGGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCg                                                                                                                         >  2:2257321/1‑150 (MQ=255)
                               cAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTTTCGCGGCTATTTTTTTAGACAGCGTGTAATTTAAATACAGCTCATCAACATGGTGAAGAGtt                                                                                                                       >  1:1674438/1‑150 (MQ=255)
                               cAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGtt                                                                                                                       <  2:5283072/150‑1 (MQ=255)
                                aaaGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTATCCTTCGTGAAATTTAAAAACAGCTCAACAAAGTTGTGAATAGTTg                                                                                                                      >  1:3309886/1‑150 (MQ=255)
                                aaaGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTAATTTTTTCGCCTGCGGGAAATTTAAATCCAGCTCAACAACGTGGTGAAGCGTTg                                                                                                                      >  1:2977264/1‑150 (MQ=255)
                                 aaGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGGGTAAATTAAATCCAGCTCATAAACGTGGTGAAGCGTTGt                                                                                                                     >  1:4444181/1‑150 (MQ=255)
                                  aGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTc                                                                                                                    <  1:2569288/150‑1 (MQ=255)
                                  aGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATAAACGTGGTGAAGCGTTGTc                                                                                                                    >  2:1945557/1‑150 (MQ=255)
                                     cTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGa                                                                                                                 <  2:4708386/150‑1 (MQ=255)
                                        aCCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTc                                                                                                              <  2:2608454/150‑1 (MQ=255)
                                           gAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTcgcg                                                                                                           >  2:2858838/1‑150 (MQ=255)
                                           gAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTcgcg                                                                                                           >  2:3742209/1‑150 (MQ=255)
                                            aaaGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTGGGATTCGCGt                                                                                                          >  1:4745916/1‑150 (MQ=255)
                                                cgcCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAAAGTGGTGAAGCGGTGTCGGGTTCGCGTGCTc                                                                                                      >  2:1867481/1‑150 (MQ=255)
                                                cgcCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCGATTTTTTTCGCCTGCGGGTAATTTAAAAACAGCGCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTc                                                                                                      >  1:2748940/1‑150 (MQ=255)
                                                    tgctgTGGCTGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCg                                                                                                  <  2:4542338/150‑1 (MQ=255)
                                                    tgctgTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCg                                                                                                  <  2:1438122/150‑1 (MQ=255)
                                                    tgctgTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCg                                                                                                  <  2:3103383/150‑1 (MQ=255)
                                                    tgctgTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCg                                                                                                  <  2:3270175/150‑1 (MQ=255)
                                                          ggATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTTTCGCGGCTATTTTTTTCGCCGGCGGGAAATTTAAATACAGCTCATCAACGTGGTGAAGCGTTGTCGGATTAGCGTGCTCATCGACGGcc                                                                                            >  1:104474/1‑149 (MQ=255)
                                                           gATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTTTCGCGGGTATTTTTTTAGCCTGCGTGTAAATTAAATACAGCTCATCAACGGGGTGAAGCGGTGTCGGATTGGCGTGCGAATAGACGGCAt                                                                                           >  1:3658190/1‑150 (MQ=255)
                                                           gATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATACAGCTCATAAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCaa                                                                                           >  1:3237887/1‑149 (MQ=255)
                                                               ggTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAAc                                                                                       >  1:849780/1‑150 (MQ=255)
                                                                gTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAAcc                                                                                      <  2:912925/150‑1 (MQ=255)
                                                                gTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAAcc                                                                                      >  2:3896775/1‑150 (MQ=255)
                                                                  aaTGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCCTCGACGGCATAAACCTg                                                                                    >  2:2962380/1‑150 (MQ=255)
                                                                  aaTGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTg                                                                                    <  1:696566/150‑1 (MQ=255)
                                                                      gTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGttt                                                                                <  1:2363542/150‑1 (MQ=255)
                                                                               cacaAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGc                                                                       <  1:1274576/150‑1 (MQ=255)
                                                                               cacaAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCAGCGTGAAATTTAAATACAGCACATCAACGTGGTGAATCGTTTTCGGATTAGCGTGCTCATCCACGGCATAAACCAGGGTTGCCGGAAGc                                                                       >  1:5312764/1‑150 (MQ=255)
                                                                                  aaaTCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTgg                                                                    <  2:1073868/150‑1 (MQ=255)
                                                                                    aTCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGaa                                                                  >  2:1779947/1‑150 (MQ=255)
                                                                                    aTCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGaa                                                                  <  2:1241526/150‑1 (MQ=255)
                                                                                     tCATTCGAGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAAc                                                                 <  1:4290734/150‑1 (MQ=255)
                                                                                       aTTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAg                                                               <  1:1535826/150‑1 (MQ=255)
                                                                                        ttAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGa                                                              >  2:3934109/1‑150 (MQ=255)
                                                                                         tAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGat                                                             <  1:2402594/150‑1 (MQ=255)
                                                                                           gctgTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGatat                                                           <  1:2610477/147‑1 (MQ=255)
                                                                                              gTTTTAAAGGTGTGGTCGATTTTTTTCGCGGCTATTTTTTTCGCCAGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCAGGTTTGCCGGAAGCTGGAACAGATATtca                                                        >  1:2687213/1‑150 (MQ=255)
                                                                                              gTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTAGCCAGCGTGAAAATTAAATCCAGCTCAACAACCTGGTGAAGCGTTTTCGGATTAGCGTGCTCAAACACGGCATAAACCAGGTTTGCCGGAAGCAGGAACAGATAttaa                                                        >  1:1744054/1‑148 (MQ=255)
                                                                                               ttttAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATtcat                                                       <  1:1376243/150‑1 (MQ=255)
                                                                                               ttttAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATtcat                                                       <  2:1874841/150‑1 (MQ=255)
                                                                                                  tAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCaa                                                    <  1:4044704/150‑1 (MQ=255)
                                                                                                     aGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAg                                                 <  1:3059586/150‑1 (MQ=255)
                                                                                                      ggTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGa                                                <  1:2132037/150‑1 (MQ=255)
                                                                                                      ggTGTGGTCGAATTGTGTCGCGGCTATATTTTTCGCCTGCGTGAAATTTAAATCCAGCACATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGa                                                <  2:1842817/150‑1 (MQ=255)
                                                                                                       gtgtGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTATCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGAt                                               <  2:2449894/150‑1 (MQ=255)
                                                                                                       gtgtGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGAt                                               <  2:4169920/150‑1 (MQ=255)
                                                                                                         gtgGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCAGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGt                                             >  2:725855/1‑150 (MQ=255)
                                                                                                            gTCGATTTTTGTCGCGGCTATTTTTTTAGCCAGCGTGAAATTTAAATACAGCTCATAAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATAGACGGCATAAAACAGGTTTGCCGGAAGCTGGAACAGATATTAATCAACAGATGTTaa                                          >  1:1869335/1‑150 (MQ=255)
                                                                                                              cGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGt                                        <  2:612968/150‑1 (MQ=255)
                                                                                                               gATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGtt                                       >  2:4178829/1‑150 (MQ=255)
                                                                                                                aTTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTg                                      <  1:3774659/150‑1 (MQ=255)
                                                                                                                aTTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTg                                      >  2:137808/1‑150 (MQ=255)
                                                                                                                aTTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTg                                      >  2:2522842/1‑150 (MQ=255)
                                                                                                                    ttGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATACAGCTCATCAACGTGGTGAAGCGTTGTCGGATTAGCGTGCTCATCGACGGCATAAAACTGGTTTGCCGGAAGCTGGAACAGATATTAATCAACAGATGTTAAGTTGCAgg                                  >  1:4509755/1‑150 (MQ=255)
                                                                                                                      gTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGtt                                >  2:810436/1‑150 (MQ=255)
                                                                                                                          cgGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAAt                            <  2:501767/150‑1 (MQ=255)
                                                                                                                          cgGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAAt                            <  2:4768494/150‑1 (MQ=255)
                                                                                                                          cgGCTATTTTTTTCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAAt                            <  2:215385/150‑1 (MQ=255)
                                                                                                                           ggCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATa                           <  1:3937541/150‑1 (MQ=255)
                                                                                                                            gCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAATAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATaa                          <  1:5308904/150‑1 (MQ=255)
                                                                                                                            gCTATTTTGTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATaa                          <  1:3867756/150‑1 (MQ=255)
                                                                                                                               aTTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGa                       <  1:3564822/150‑1 (MQ=255)
                                                                                                                               aTTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGa                       >  2:1562542/1‑150 (MQ=255)
                                                                                                                                 ttttttCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATg                     <  1:3158452/150‑1 (MQ=255)
                                                                                                                                 ttttttCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATg                     <  2:3189437/150‑1 (MQ=255)
                                                                                                                                   ttttCGCCTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGaa                   <  2:902680/150‑1 (MQ=255)
                                                                                                                                    tttCGCCTGCGTGAAATTTAAATCCAGCTCATCATCGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAAt                  <  2:517184/150‑1 (MQ=255)
                                                                                                                                    tttCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAAt                  <  1:3975129/150‑1 (MQ=255)
                                                                                                                                    tttCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAAt                  <  2:353224/150‑1 (MQ=255)
                                                                                                                                      tCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATAc                <  2:4500363/150‑1 (MQ=255)
                                                                                                                                       cGCCTGCGGGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGACGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATAcc               >  2:4344165/1‑150 (MQ=255)
                                                                                                                                         ccTGCGTGAAAATTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCa             <  2:1438882/150‑1 (MQ=255)
                                                                                                                                           tGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCACg           >  1:264921/1‑150 (MQ=255)
                                                                                                                                               tGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCACGCCAt       >  2:1118725/1‑150 (MQ=255)
                                                                                                                                                   aTTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAgg   >  2:3549349/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GCTATAAACCTGCACCCAGAGTTGATCGGAACAAAGGCTTACCGAAAGCGCCTGCTGTGGATGGGTAATGGTCATTTCCCACAAATCATTAGCGGTTTTAAAGGTGTGGTCGATTTTTGTCGCGGCTATTTTTTTCGCCTGCGTGAAATTTAAATCCAGCTCATCAACGTGGTGAAGCGTTGTCGGATTCGCGTGCTCATCGACGGCATAAACCTGGTTTGCCGGAAGCTGGAACAGATATTCATCAACAGATGTTAAGTTGCAGGTTAAATAAGGATGAATACCCACGCCATAAGGC  >  CP009273/4062979‑4063276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A