Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,618,693 G→T 100% E74* (GAA→TAA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,618,6930GT99.1% 836.5 / ‑2.1 256E74* (GAA→TAA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (114/138);  total (114/142)
Fisher's exact test for biased strand distribution p-value = 1.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 256 total aligned reads displayed.

GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGC  >  CP009273/2618548‑2618838
                                                                                                                                                 |                                                                                                                                                 
ggTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAAc                                                                                                                                                >  1:1033706/1‑149 (MQ=255)
   cATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGc                                                                                                                                             <  2:470938/149‑1 (MQ=255)
    aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATCATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                            >  1:1045870/1‑149 (MQ=255)
    aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                            <  1:2014673/149‑1 (MQ=255)
     tAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTa                                                                                                                                           >  2:2423869/1‑149 (MQ=255)
     tAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTa                                                                                                                                           >  2:2387225/1‑149 (MQ=255)
        cccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACaa                                                                                                                                        <  1:3440805/149‑1 (MQ=255)
         ccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAg                                                                                                                                       >  2:744400/1‑149 (MQ=255)
           gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAggg                                                                                                                                     >  1:1858070/1‑149 (MQ=255)
            tgtgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGt                                                                                                                                    >  1:3386856/1‑149 (MQ=255)
              tgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGttt                                                                                                                                  >  1:384404/1‑149 (MQ=255)
              tgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGttt                                                                                                                                  <  1:474035/149‑1 (MQ=255)
                  aGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTtctc                                                                                                                              <  2:781734/149‑1 (MQ=255)
                   gATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTcc                                                                                                                             <  2:2210312/149‑1 (MQ=255)
                    aTGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCt                                                                                                                            >  1:768588/1‑149 (MQ=255)
                    aTGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCt                                                                                                                            >  1:3077106/1‑149 (MQ=255)
                     tGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTg                                                                                                                           >  2:3004020/1‑149 (MQ=255)
                     tGGTGCCAAGCAGATTATTGGCCTCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTg                                                                                                                           >  1:4092110/1‑149 (MQ=255)
                      ggTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGc                                                                                                                          >  2:612705/1‑149 (MQ=255)
                       gTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGcc                                                                                                                         <  1:3560426/149‑1 (MQ=255)
                             tGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                   >  1:849577/1‑149 (MQ=255)
                             tGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTc                                                                                                                   <  2:2046209/149‑1 (MQ=255)
                                  attattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                              <  1:2948766/149‑1 (MQ=255)
                                  attattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                              >  1:2059100/1‑149 (MQ=255)
                                      ttGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                                                                                                          <  1:1121751/149‑1 (MQ=255)
                                             ccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAccc                                                                                                   <  1:2632475/149‑1 (MQ=255)
                                              cTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCa                                                                                                  <  2:1633851/149‑1 (MQ=255)
                                                gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATa                                                                                                <  2:2155554/149‑1 (MQ=255)
                                                gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATa                                                                                                >  1:3256064/1‑149 (MQ=255)
                                                 aaaCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAc                                                                                               >  2:1901337/1‑149 (MQ=255)
                                                 aaaCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAc                                                                                               >  2:3703567/1‑149 (MQ=255)
                                                 aaaCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTGTCTCCTGCCAGCGTCTGTCTAGTTGGTACCCATAc                                                                                               >  2:3939129/1‑149 (MQ=255)
                                                   aCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGc                                                                                             >  2:816476/1‑149 (MQ=255)
                                                   aCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGc                                                                                             <  2:754093/149‑1 (MQ=255)
                                                   aCAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGc                                                                                             <  2:1352633/149‑1 (MQ=255)
                                                    cAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGGGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCt                                                                                            <  2:4098/149‑1 (MQ=255)
                                                         ggTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTc                                                                                       >  2:2973173/1‑149 (MQ=255)
                                                           tGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAg                                                                                     <  1:3170430/149‑1 (MQ=255)
                                                           tGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAg                                                                                     <  2:1091371/149‑1 (MQ=255)
                                                               tCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgcgc                                                                                 <  2:1664410/149‑1 (MQ=255)
                                                               tCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgcgc                                                                                 <  1:1149822/149‑1 (MQ=255)
                                                                   gCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGaa                                                                             >  2:1028141/1‑149 (MQ=255)
                                                                    cGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAAc                                                                            <  1:1330348/149‑1 (MQ=255)
                                                                    cGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAAc                                                                            <  2:214754/149‑1 (MQ=255)
                                                                      gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          >  1:1683385/1‑149 (MQ=255)
                                                                      gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          >  1:3728169/1‑149 (MQ=255)
                                                                      gACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                          >  1:2388660/1‑149 (MQ=255)
                                                                       aCGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGcc                                                                         <  1:301495/149‑1 (MQ=255)
                                                                            cTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGa                                                                    >  2:1082649/1‑149 (MQ=255)
                                                                             tGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAc                                                                   <  1:154521/149‑1 (MQ=255)
                                                                               gggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACtt                                                                 <  2:3219692/149‑1 (MQ=255)
                                                                                ggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACttt                                                                >  2:518196/1‑149 (MQ=255)
                                                                                ggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACttt                                                                >  2:50444/1‑149 (MQ=255)
                                                                                 gCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTc                                                               <  2:666615/149‑1 (MQ=255)
                                                                                 gCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTc                                                               <  1:2341139/149‑1 (MQ=255)
                                                                                  ccAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCt                                                              >  2:1464729/1‑149 (MQ=255)
                                                                                   cAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTg                                                             <  1:4105219/149‑1 (MQ=255)
                                                                                     gATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGaa                                                           >  2:1324384/1‑149 (MQ=255)
                                                                                     gATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGaa                                                           >  2:406683/1‑149 (MQ=255)
                                                                                        aataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcg                                                        >  2:1414918/1‑149 (MQ=255)
                                                                                           atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                     <  1:3690189/149‑1 (MQ=255)
                                                                                           atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                     <  1:2805205/149‑1 (MQ=255)
                                                                                           atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                     <  1:744400/149‑1 (MQ=255)
                                                                                                gAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaa                                                >  2:134878/1‑149 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaaa                                               >  1:1176363/1‑149 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaaa                                               <  1:197215/149‑1 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaaa                                               <  2:3594016/149‑1 (MQ=255)
                                                                                                 aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaaaa                                               >  1:659148/1‑149 (MQ=255)
                                                                                                   tGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAgg                                             <  2:1610843/149‑1 (MQ=255)
                                                                                                    gAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGt                                            <  1:1925433/149‑1 (MQ=255)
                                                                                                     aaGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTc                                           <  2:4108491/149‑1 (MQ=255)
                                                                                                       gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAt                                         <  1:2509550/149‑1 (MQ=255)
                                                                                                        agGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAtt                                        <  2:381385/149‑1 (MQ=255)
                                                                                                          gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTcc                                      >  2:1998165/1‑149 (MQ=255)
                                                                                                          gCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCAGCCAGCGTCTGTAGAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTcc                                      >  2:1998396/1‑149 (MQ=255)
                                                                                                            aaTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGACCGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCt                                    >  2:2756092/1‑149 (MQ=255)
                                                                                                            aaTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCt                                    >  1:1429853/1‑149 (MQ=255)
                                                                                                             aTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTa                                   >  2:3333245/1‑149 (MQ=255)
                                                                                                             aTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTa                                   <  2:311695/149‑1 (MQ=255)
                                                                                                                  gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGa                              <  2:3434384/149‑1 (MQ=255)
                                                                                                                  gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGa                              <  2:365644/149‑1 (MQ=255)
                                                                                                                    gcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGAtt                            <  2:4014822/149‑1 (MQ=255)
                                                                                                                    gcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGAtt                            >  2:3769352/1‑149 (MQ=255)
                                                                                                                     cgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTg                           <  1:3628019/149‑1 (MQ=255)
                                                                                                                     cgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTg                           <  2:3686941/149‑1 (MQ=255)
                                                                                                                      ggTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGa                          >  2:964078/1‑149 (MQ=255)
                                                                                                                       gTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGaa                         >  2:3412052/1‑149 (MQ=255)
                                                                                                                          tAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                      >  1:129236/1‑149 (MQ=255)
                                                                                                                          tAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                      >  2:2343568/1‑149 (MQ=255)
                                                                                                                          tAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACAGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                      >  1:129167/1‑149 (MQ=255)
                                                                                                                            aaCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattat                    <  2:257279/149‑1 (MQ=255)
                                                                                                                             aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAattatt                   >  1:581741/1‑149 (MQ=255)
                                                                                                                              ctgtctgtCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTAttt                  <  2:499175/149‑1 (MQ=255)
                                                                                                                               tgtctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTc                 >  2:2520228/1‑149 (MQ=255)
                                                                                                                                   tgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAATGGTCATTCCCTACCCGATTGAAATTATTTCCGGt             <  2:3638747/149‑1 (MQ=255)
                                                                                                                                     tcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTaa           <  1:2678042/149‑1 (MQ=255)
                                                                                                                                     tcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTaa           <  2:1045870/149‑1 (MQ=255)
                                                                                                                                       gCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATg         <  2:3345271/149‑1 (MQ=255)
                                                                                                                                          gTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaag      <  2:3614162/149‑1 (MQ=255)
                                                                                                                                              gCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGc  >  1:1827978/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGC  >  CP009273/2618548‑2618838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCCGT  >  CP009273/2618544‑2618842
                                                                                                                                                     |                                                                                                                                                     
ACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCT                                                                                                                                                       <  SRR19973068.1363092/150‑1 (MQ=60)
   TGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAAC                                                                                                                                                    >  SRR19973068.1040355/1‑150 (MQ=60)
    GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACG                                                                                                                                                   <  SRR19973068.2605126/150‑1 (MQ=60)
       CATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATCATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCT                                                                                                                                                >  SRR19973068.1052613/1‑150 (MQ=60)
       CATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCT                                                                                                                                                <  SRR19973068.2400761/150‑1 (MQ=60)
        ATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTA                                                                                                                                               <  SRR19973068.2027904/150‑1 (MQ=60)
        ATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTA                                                                                                                                               <  SRR19973068.2027914/150‑1 (MQ=60)
           GCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAA                                                                                                                                            >  SRR19973068.1206052/1‑150 (MQ=60)
            CCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAG                                                                                                                                           <  SRR19973068.807283/150‑1 (MQ=60)
            CCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAG                                                                                                                                           <  SRR19973068.3461530/150‑1 (MQ=60)
             CCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGG                                                                                                                                          <  SRR19973068.3192221/150‑1 (MQ=60)
              CGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGG                                                                                                                                         >  SRR19973068.1870352/1‑150 (MQ=60)
               GTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGT                                                                                                                                        >  SRR19973068.3407394/1‑150 (MQ=60)
                 GTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTT                                                                                                                                      >  SRR19973068.386941/1‑150 (MQ=60)
                 GTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTT                                                                                                                                      >  SRR19973068.387057/1‑150 (MQ=60)
                  TGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTT                                                                                                                                     <  SRR19973068.477368/150‑1 (MQ=60)
                  TGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTT                                                                                                                                     <  SRR19973068.477426/150‑1 (MQ=60)
                  TGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTT                                                                                                                                     <  SRR19973068.478812/150‑1 (MQ=60)
                  TGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTT                                                                                                                                     <  SRR19973068.1051530/150‑1 (MQ=60)
                  TGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTT                                                                                                                                     <  SRR19973068.477381/150‑1 (MQ=60)
                   GGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTC                                                                                                                                    >  SRR19973068.3511600/1‑150 (MQ=60)
                    GTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCT                                                                                                                                   >  SRR19973068.96013/1‑150 (MQ=60)
                       GATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCT                                                                                                                                >  SRR19973068.3095878/1‑150 (MQ=60)
                       GATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCT                                                                                                                                >  SRR19973068.773783/1‑150 (MQ=60)
                        ATGGTGCCATGCAGATTATTGGCCTCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTG                                                                                                                               >  SRR19973068.4116687/1‑150 (MQ=60)
                        ATGGTGCCATGCAGATTATTGGCCTCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTCGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTGTCTCCTG                                                                                                                               >  SRR19973068.4116677/1‑150 (MQ=60)
                        ATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTG                                                                                                                               >  SRR19973068.368086/1‑150 (MQ=60)
                        ATGGTGCCAAGCAGATTATTGGCCTCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTG                                                                                                                               >  SRR19973068.4116644/1‑150 (MQ=60)
                           GTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCA                                                                                                                            <  SRR19973068.3582063/150‑1 (MQ=60)
                           GTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCA                                                                                                                            <  SRR19973068.3582050/150‑1 (MQ=60)
                                ATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTC                                                                                                                       >  SRR19973068.855359/1‑150 (MQ=60)
                                ATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTC                                                                                                                       >  SRR19973068.854927/1‑150 (MQ=60)
                                    AGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTA                                                                                                                   >  SRR19973068.3719654/1‑150 (MQ=60)
                                     GATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTAT                                                                                                                  >  SRR19973068.2072492/1‑150 (MQ=60)
                                      ATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATA                                                                                                                 >  SRR19973068.116439/1‑150 (MQ=60)
                                      ATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATA                                                                                                                 >  SRR19973068.670881/1‑150 (MQ=60)
                                      ATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATA                                                                                                                 <  SRR19973068.2967060/150‑1 (MQ=60)
                                        TATTGGCCGCCTGAAACAGCGGGTGCATCTGACGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGT                                                                                                               >  SRR19973068.1323889/1‑150 (MQ=60)
                                          TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                             <  SRR19973068.2004171/150‑1 (MQ=60)
                                          TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                             <  SRR19973068.1363204/150‑1 (MQ=60)
                                          TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                             <  SRR19973068.2005277/150‑1 (MQ=60)
                                          TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                             <  SRR19973068.1129247/150‑1 (MQ=60)
                                          TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                             <  SRR19973068.3192153/150‑1 (MQ=60)
                                               CGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACC                                                                                                        >  SRR19973068.1391039/1‑150 (MQ=60)
                                               CGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACC                                                                                                        <  SRR19973068.2707611/150‑1 (MQ=60)
                                                 CCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCA                                                                                                      <  SRR19973068.2649022/150‑1 (MQ=60)
                                                  CTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTCCAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCAT                                                                                                     >  SRR19973068.2217503/1‑150 (MQ=60)
                                                   TGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATA                                                                                                    >  SRR19973068.3276071/1‑150 (MQ=60)
                                                           CGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGT                                                                                            >  SRR19973068.3985931/1‑150 (MQ=60)
                                                             GGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCA                                                                                          <  SRR19973068.855396/150‑1 (MQ=60)
                                                              GTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAG                                                                                         >  SRR19973068.313744/1‑150 (MQ=60)
                                                              GTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAG                                                                                         >  SRR19973068.2397315/1‑150 (MQ=60)
                                                               TGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGG                                                                                        <  SRR19973068.3189930/150‑1 (MQ=60)
                                                                 CATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCG                                                                                      >  SRR19973068.1499101/1‑150 (MQ=60)
                                                                 CATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCG                                                                                      >  SRR19973068.744840/1‑150 (MQ=60)
                                                                   TCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCT                                                                                    <  SRR19973068.1157517/150‑1 (MQ=60)
                                                                    CTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTG                                                                                   <  SRR19973068.1139073/150‑1 (MQ=60)
                                                                     TGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGA                                                                                  <  SRR19973068.3852858/150‑1 (MQ=60)
                                                                        CGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACG                                                                               <  SRR19973068.2990001/150‑1 (MQ=60)
                                                                        CGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACG                                                                               <  SRR19973068.1338964/150‑1 (MQ=60)
                                                                         GGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGC                                                                              >  SRR19973068.1694213/1‑150 (MQ=60)
                                                                         GGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGC                                                                              >  SRR19973068.3750546/1‑150 (MQ=60)
                                                                         GGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGC                                                                              >  SRR19973068.2403883/1‑150 (MQ=60)
                                                                           ACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCA                                                                            <  SRR19973068.291165/150‑1 (MQ=60)
                                                                           ACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCA                                                                            <  SRR19973068.303507/150‑1 (MQ=60)
                                                                                CTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAC                                                                       <  SRR19973068.2657377/150‑1 (MQ=60)
                                                                                 TGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACT                                                                      <  SRR19973068.155825/150‑1 (MQ=60)
                                                                                 TGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACT                                                                      <  SRR19973068.2243349/150‑1 (MQ=60)
                                                                                   GGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTT                                                                    <  SRR19973068.60751/150‑1 (MQ=60)
                                                                                     GCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCT                                                                  <  SRR19973068.1076746/150‑1 (MQ=60)
                                                                                     GCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCT                                                                  <  SRR19973068.2356171/150‑1 (MQ=60)
                                                                                      CCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTG                                                                 >  SRR19973068.1853857/1‑150 (MQ=60)
                                                                                       CAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGA                                                                <  SRR19973068.4129788/150‑1 (MQ=60)
                                                                                              TATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTTTCTGTCGCAGTTTGCCTAACTGCTACAATGGTTTTCTCATGCCAGCTTCAGTATATTTGGTAACCATAatctccgtcatgcgctgaaccacactgaaatactgaaaaacgc                                                         >  SRR19973068.1961744/1‑107 (MQ=60)
                                                                                               ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                        <  SRR19973068.749374/150‑1 (MQ=60)
                                                                                               ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                        <  SRR19973068.2822637/150‑1 (MQ=60)
                                                                                               ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                        <  SRR19973068.3712732/150‑1 (MQ=60)
                                                                                               ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                        <  SRR19973068.3712431/150‑1 (MQ=60)
                                                                                               ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                        <  SRR19973068.749370/150‑1 (MQ=60)
                                                                                                    GAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAA                                                   >  SRR19973068.1182973/1‑150 (MQ=60)
                                                                                                    GAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAA                                                   >  SRR19973068.1184233/1‑150 (MQ=60)
                                                                                                    GAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAA                                                   >  SRR19973068.663398/1‑150 (MQ=60)
                                                                                                     AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAG                                                  <  SRR19973068.198745/150‑1 (MQ=60)
                                                                                                        GAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTC                                               <  SRR19973068.1938238/150‑1 (MQ=60)
                                                                                                        GAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTC                                               <  SRR19973068.1938220/150‑1 (MQ=60)
                                                                                                           GAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATT                                            <  SRR19973068.2525530/150‑1 (MQ=60)
                                                                                                           GAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATT                                            <  SRR19973068.2760892/150‑1 (MQ=60)
                                                                                                               CAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCT                                        >  SRR19973068.1894172/1‑150 (MQ=60)
                                                                                                               CAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCT                                        >  SRR19973068.1439194/1‑150 (MQ=60)
                                                                                                                AATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTA                                       >  SRR19973068.3061096/1‑150 (MQ=60)
                                                                                                                       CGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATT                                <  SRR19973068.1570535/150‑1 (MQ=60)
                                                                                                                       CGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATT                                <  SRR19973068.3450915/150‑1 (MQ=60)
                                                                                                                         CGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGA                              <  SRR19973068.3650018/150‑1 (MQ=60)
                                                                                                                         CGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGA                              <  SRR19973068.3650008/150‑1 (MQ=60)
                                                                                                                             TTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATT                          >  SRR19973068.130353/1‑150 (MQ=60)
                                                                                                                             TTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATT                          >  SRR19973068.130419/1‑150 (MQ=60)
                                                                                                                             TTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAAGCGCGGAAAAGGTCATTACCTACCCGATTGAAATT                          >  SRR19973068.130381/1‑150 (MQ=60)
                                                                                                                             TTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACAGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATT                          >  SRR19973068.130349/1‑150 (MQ=60)
                                                                                                                             TTAAACTGTCTGTCGCTGTTTGCCTAAAGGCTAAAAGGGTTTTCTCCTGCCAGAGTCTGTATATTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTACCTACCCTATTGAAATT                          >  SRR19973068.1066217/1‑150 (MQ=60)
                                                                                                                                AACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATT                       >  SRR19973068.585715/1‑150 (MQ=60)
                                                                                                                                AACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATT                       <  SRR19973068.1751096/150‑1 (MQ=60)
                                                                                                                                AACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATT                       <  SRR19973068.1751095/150‑1 (MQ=60)
                                                                                                                                   TGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCC                    <  SRR19973068.2439237/150‑1 (MQ=60)
                                                                                                                                    GTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCG                   <  SRR19973068.3036329/150‑1 (MQ=60)
                                                                                                                                       TGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTA                <  SRR19973068.2987525/150‑1 (MQ=60)
                                                                                                                                        GTCGCTGTTTGCCTAACGTCTACAAGGTTTTTCTCCTGACAGCGTATGTATAGTTGGTACCCATACGCTGCGTCAGGCTCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAA               <  SRR19973068.1516844/150‑1 (MQ=60)
                                                                                                                                         TCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAAT              <  SRR19973068.2694738/150‑1 (MQ=60)
                                                                                                                                             TGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAG          >  SRR19973068.3380985/1‑150 (MQ=60)
                                                                                                                                                TTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAG       <  SRR19973068.3414892/150‑1 (MQ=60)
                                                                                                                                                TTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAG       <  SRR19973068.2560699/150‑1 (MQ=60)
                                                                                                                                                 TGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGC      >  SRR19973068.1840022/1‑150 (MQ=60)
                                                                                                                                                   CCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC    <  SRR19973068.3353583/150‑1 (MQ=60)
                                                                                                                                                   CCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC    >  SRR19973068.1907850/1‑150 (MQ=60)
                                                                                                                                                    CTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCCG   <  SRR19973068.3079057/150‑1 (MQ=60)
                                                                                                                                                     TAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCCGT  >  SRR19973068.2769020/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
ACATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCCGT  >  CP009273/2618544‑2618842

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑