Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,618,693 G→T 54.3% E74* (GAA→TAA)  ppx → exopolyphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,618,6930GT54.3% 18.0 / inf 208E74* (GAA→TAA) ppxexopolyphosphatase
Reads supporting (aligned to +/- strand):  ref base G (36/59);  new base T (47/65);  total (84/124)
Fisher's exact test for biased strand distribution p-value = 5.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

Only 100 of 208 total aligned reads displayed.

ATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC  >  CP009273/2618546‑2618840
                                                                                                                                                   |                                                                                                                                                   
aTGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTaa                                                                                                                                                   >  1:3702387/1‑150 (MQ=255)
aTGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGaa                                                                                                                                                   >  1:190375/1‑150 (MQ=255)
  ggTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACg                                                                                                                                                 >  1:3707060/1‑150 (MQ=255)
   gTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACgg                                                                                                                                                >  1:521698/1‑150 (MQ=255)
     cATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCt                                                                                                                                              <  2:136926/150‑1 (MQ=255)
      aTAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTa                                                                                                                                             >  1:1343205/1‑150 (MQ=255)
       tAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTAc                                                                                                                                            >  1:1590377/1‑150 (MQ=255)
        aGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACa                                                                                                                                           <  1:864142/150‑1 (MQ=255)
        aGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTACATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACa                                                                                                                                           >  2:1424927/1‑150 (MQ=255)
          cccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAg                                                                                                                                         >  2:3880732/1‑150 (MQ=255)
           ccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAgg                                                                                                                                        >  1:2669889/1‑150 (MQ=255)
           ccGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAgg                                                                                                                                        <  1:2325512/150‑1 (MQ=255)
             gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGt                                                                                                                                      <  2:1590239/150‑1 (MQ=255)
             gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGt                                                                                                                                      <  2:3163922/150‑1 (MQ=255)
             gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGt                                                                                                                                      <  1:2190893/150‑1 (MQ=255)
             gtgtGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGt                                                                                                                                      <  2:577067/150‑1 (MQ=255)
              tgtgGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGtt                                                                                                                                     <  2:3008326/150‑1 (MQ=255)
                 ggTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTtc                                                                                                                                  >  2:3049558/1‑150 (MQ=255)
                 ggTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTtc                                                                                                                                  <  2:3571120/150‑1 (MQ=255)
                      aTGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTg                                                                                                                             >  2:2236945/1‑150 (MQ=255)
                      aTGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTg                                                                                                                             >  1:2701566/1‑150 (MQ=255)
                       tGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGc                                                                                                                            <  1:1083233/150‑1 (MQ=255)
                       tGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGc                                                                                                                            <  1:2260513/150‑1 (MQ=255)
                       tGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGc                                                                                                                            <  1:1083266/150‑1 (MQ=255)
                         gTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCa                                                                                                                          <  1:1313536/150‑1 (MQ=255)
                             cATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAATGGCTACAAGGGTTTTCTCCTGCCAGCGt                                                                                                                      >  1:3095965/1‑150 (MQ=255)
                               tGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCt                                                                                                                    <  1:2425531/150‑1 (MQ=255)
                                 cAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGt                                                                                                                  <  2:4022625/150‑1 (MQ=255)
                                   gATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                                <  2:4143948/150‑1 (MQ=255)
                                   gATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGtat                                                                                                                <  1:1859063/150‑1 (MQ=255)
                                     ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                              <  2:3443683/150‑1 (MQ=255)
                                     ttattGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAg                                                                                                              <  2:2856285/150‑1 (MQ=255)
                                       attGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGtt                                                                                                            >  2:3818916/1‑150 (MQ=255)
                                          ggCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGt                                                                                                         >  1:1807006/1‑150 (MQ=255)
                                          ggCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGt                                                                                                         >  2:2036191/1‑150 (MQ=255)
                                            ccgccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAc                                                                                                       >  1:3137413/1‑150 (MQ=255)
                                             cgccTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAcc                                                                                                      <  2:1526129/150‑1 (MQ=255)
                                                cTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCAt                                                                                                   >  2:889325/1‑150 (MQ=255)
                                                  gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTACACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTTTAGTTGGTACCCATAc                                                                                                 <  1:3040879/150‑1 (MQ=255)
                                                  gAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAc                                                                                                 <  1:1662087/150‑1 (MQ=255)
                                                          gggTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTc                                                                                         <  1:1533963/150‑1 (MQ=255)
                                                              gCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgc                                                                                     >  2:3747413/1‑150 (MQ=255)
                                                               cATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGgcg                                                                                    <  1:669267/150‑1 (MQ=255)
                                                                 tCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCt                                                                                  <  1:19455/150‑1 (MQ=255)
                                                                 tCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCt                                                                                  <  2:2694968/150‑1 (MQ=255)
                                                                   tGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGa                                                                                >  1:2085825/1‑150 (MQ=255)
                                                                      cGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCCATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACg                                                                             >  2:3856638/1‑150 (MQ=255)
                                                                       ggACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGc                                                                            >  1:505254/1‑150 (MQ=255)
                                                                           ggCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACt                                                                        <  2:3997367/150‑1 (MQ=255)
                                                                              cTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAc                                                                     <  1:3474364/150‑1 (MQ=255)
                                                                               tGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACt                                                                    >  2:2781067/1‑150 (MQ=255)
                                                                                 gggCCAGATAATATGTTGAGTGAAGAGGCAATGACGAGCGGTTTAAACTGTCTGTCGCTGTTTGCCTATCGGCTACAAGGGTTTTCTCCTGACAGCGTCTGTTTAGTTGGTTCCCAGACGCTGCGTCAGGCGATGAACGCCACTGACttt                                                                  <  2:1353253/150‑1 (MQ=255)
                                                                                 gggCAAGATAATCTGTTGAGTGTATAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACttt                                                                  <  2:946512/150‑1 (MQ=255)
                                                                                  ggCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTc                                                                 <  2:3427119/150‑1 (MQ=255)
                                                                                   gCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCt                                                                <  2:1500324/150‑1 (MQ=255)
                                                                                   gCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCt                                                                <  1:229976/150‑1 (MQ=255)
                                                                                    ccAGATAATATGTTTAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTATGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGTTACCAATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTg                                                               >  2:659224/1‑150 (MQ=255)
                                                                                      aGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGaa                                                             >  1:847954/1‑150 (MQ=255)
                                                                                        ataataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAc                                                           <  2:1628732/150‑1 (MQ=255)
                                                                                        ataataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAc                                                           <  2:2389290/150‑1 (MQ=255)
                                                                                        ataataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAc                                                           >  1:1074783/1‑150 (MQ=255)
                                                                                         taataTGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcg                                                          <  1:1462568/150‑1 (MQ=255)
                                                                                           atatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcg                                                        <  2:2863170/150‑1 (MQ=255)
                                                                                            tatGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgc                                                       >  1:2125058/1‑150 (MQ=255)
                                                                                             atGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAcgcgcg                                                      >  1:531023/1‑150 (MQ=255)
                                                                                               gTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGa                                                    >  1:1115908/1‑150 (MQ=255)
                                                                                                ttGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGaa                                                   <  2:2995732/150‑1 (MQ=255)
                                                                                                   aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGGCTTTCTGAAACGCGCGGAAAAg                                                <  1:2076850/150‑1 (MQ=255)
                                                                                                   aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAg                                                >  1:3207901/1‑150 (MQ=255)
                                                                                                   aGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTGTCTCCTGCCAGCGTCTGTCTAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGACACGCGCGGAAAAg                                                >  1:3142888/1‑150 (MQ=255)
                                                                                                       aaGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCa                                            <  1:1269222/150‑1 (MQ=255)
                                                                                                         gagGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAtt                                          <  2:1088863/150‑1 (MQ=255)
                                                                                                          agGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTc                                         <  2:1987697/150‑1 (MQ=255)
                                                                                                           ggCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTTAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTcc                                        <  2:1972206/150‑1 (MQ=255)
                                                                                                           ggCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTcc                                        <  1:2027501/150‑1 (MQ=255)
                                                                                                             cAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCt                                      <  1:3052919/150‑1 (MQ=255)
                                                                                                              aaTGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGTGTTTTCTCCTGCCAGCGTCTGTATTGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTa                                     <  1:1010877/150‑1 (MQ=255)
                                                                                                                 gACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTAccc                                  <  2:2421134/150‑1 (MQ=255)
                                                                                                                    gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGAt                               <  2:314784/150‑1 (MQ=255)
                                                                                                                    gcgcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGAt                               <  2:314860/150‑1 (MQ=255)
                                                                                                                     cgcgGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGAtt                              <  1:3290511/150‑1 (MQ=255)
                                                                                                                      gcgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTg                             <  2:2978473/150‑1 (MQ=255)
                                                                                                                       cgGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGa                            <  2:1707447/150‑1 (MQ=255)
                                                                                                                           ttAAACGGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatt                        <  2:3589599/150‑1 (MQ=255)
                                                                                                                            tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatta                       >  2:100614/1‑150 (MQ=255)
                                                                                                                            tAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAatta                       >  2:2116354/1‑150 (MQ=255)
                                                                                                                               aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTAttt                    >  2:732266/1‑150 (MQ=255)
                                                                                                                               aCTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGGATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTAttt                    >  2:822601/1‑150 (MQ=255)
                                                                                                                                 tgtctgtcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTcc                  <  2:521698/150‑1 (MQ=255)
                                                                                                                                   tctgtcGCTGTTTGCCGAACGGATACTAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCgg                <  1:1214755/150‑1 (MQ=255)
                                                                                                                                    ctgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGt               <  1:4034691/150‑1 (MQ=255)
                                                                                                                                     tgtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTa              >  2:474321/1‑150 (MQ=255)
                                                                                                                                      gtcGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTaa             <  2:1901782/150‑1 (MQ=255)
                                                                                                                                       tcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAAt            <  2:29730/150‑1 (MQ=255)
                                                                                                                                       tcGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAAt            <  1:2498525/150‑1 (MQ=255)
                                                                                                                                        cGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATg           <  1:718385/150‑1 (MQ=255)
                                                                                                                                         gCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTTAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATga          <  2:629222/150‑1 (MQ=255)
                                                                                                                                         gCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATga          <  2:2475372/150‑1 (MQ=255)
                                                                                                                                             tttGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATgaagaa      <  2:3566938/150‑1 (MQ=255)
                                                                                                                                              ttGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGaagaag     >  1:3445050/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC  >  CP009273/2618546‑2618840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC  >  CP009273/2618545‑2618840
                                                                                                                                                    |                                                                                                                                                   
CATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTA                                                                                                                                                    >  SRR19973074.3274475/1‑150 (MQ=60)
 ATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAA                                                                                                                                                   <  SRR19973074.3894854/150‑1 (MQ=60)
 ATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAA                                                                                                                                                   >  SRR19973074.3709230/1‑150 (MQ=60)
 ATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAA                                                                                                                                                   >  SRR19973074.190780/1‑150 (MQ=60)
   GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACG                                                                                                                                                 >  SRR19973074.3713910/1‑150 (MQ=60)
   GGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACG                                                                                                                                                 >  SRR19973074.3713905/1‑150 (MQ=60)
    GTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGG                                                                                                                                                >  SRR19973074.522738/1‑150 (MQ=60)
     TCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGC                                                                                                                                               <  SRR19973074.2362851/150‑1 (MQ=60)
       ATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTA                                                                                                                                             >  SRR19973074.1345713/1‑150 (MQ=60)
        TAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTAC                                                                                                                                            >  SRR19973074.1593406/1‑150 (MQ=60)
        TAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTAC                                                                                                                                            >  SRR19973074.1593011/1‑150 (MQ=60)
         AGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACA                                                                                                                                           <  SRR19973074.865810/150‑1 (MQ=60)
           CCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAG                                                                                                                                         <  SRR19973074.2098572/150‑1 (MQ=60)
            CCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGG                                                                                                                                        <  SRR19973074.2329844/150‑1 (MQ=60)
            CCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGG                                                                                                                                        <  SRR19973074.2329854/150‑1 (MQ=60)
            CCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGG                                                                                                                                        >  SRR19973074.2674863/1‑150 (MQ=60)
              GTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGT                                                                                                                                      <  SRR19973074.2195003/150‑1 (MQ=60)
              GTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGT                                                                                                                                      <  SRR19973074.1669661/150‑1 (MQ=60)
                  GGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTC                                                                                                                                  <  SRR19973074.1507221/150‑1 (MQ=60)
                       ATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTG                                                                                                                             >  SRR19973074.2706580/1‑150 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            >  SRR19973074.4079124/1‑150 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            <  SRR19973074.2264758/150‑1 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            <  SRR19973074.2264768/150‑1 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            <  SRR19973074.1085293/150‑1 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            <  SRR19973074.1085260/150‑1 (MQ=60)
                        TGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGC                                                                                                                            <  SRR19973074.1085294/150‑1 (MQ=60)
                         GGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCC                                                                                                                           >  SRR19973074.1083282/1‑150 (MQ=60)
                          GTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCA                                                                                                                          <  SRR19973074.1316004/150‑1 (MQ=60)
                              CATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAATGGCTACAAGGGTTTTCTCCTGCCAGCGT                                                                                                                      >  SRR19973074.3101570/1‑150 (MQ=60)
                              CATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAATGGCTACAAGGGTTTTCTCCTGCCAGCGT                                                                                                                      >  SRR19973074.3101650/1‑150 (MQ=60)
                                TGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCT                                                                                                                    <  SRR19973074.2430018/150‑1 (MQ=60)
                                TGCAGATGATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACGGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCT                                                                                                                    <  SRR19973074.345111/150‑1 (MQ=60)
                                 GCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTG                                                                                                                   <  SRR19973074.3693786/150‑1 (MQ=60)
                                    GATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTAT                                                                                                                <  SRR19973074.1862619/150‑1 (MQ=60)
                                         TTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTG                                                                                                           <  SRR19973074.1101407/150‑1 (MQ=60)
                                           GGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGT                                                                                                         >  SRR19973074.1810446/1‑150 (MQ=60)
                                             CCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTAC                                                                                                       >  SRR19973074.3143166/1‑150 (MQ=60)
                                              CGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACC                                                                                                      >  SRR19973074.2185872/1‑150 (MQ=60)
                                                  TGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATA                                                                                                  >  SRR19973074.1064406/1‑150 (MQ=60)
                                                   GAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTACACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTTTAGTTGGTACCCATAC                                                                                                 <  SRR19973074.3046502/150‑1 (MQ=60)
                                                   GAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATAC                                                                                                 <  SRR19973074.1665224/150‑1 (MQ=60)
                                                           GGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTC                                                                                         <  SRR19973074.1536870/150‑1 (MQ=60)
                                                              TGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGG                                                                                      >  SRR19973074.1920532/1‑150 (MQ=60)
                                                                CATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCG                                                                                    <  SRR19973074.670545/150‑1 (MQ=60)
                                                                CATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCG                                                                                    <  SRR19973074.670501/150‑1 (MQ=60)
                                                                 ATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGC                                                                                   >  SRR19973074.529932/1‑150 (MQ=60)
                                                                  TCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCT                                                                                  <  SRR19973074.19510/150‑1 (MQ=60)
                                                                    TGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGA                                                                                >  SRR19973074.2089731/1‑150 (MQ=60)
                                                                        GGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGC                                                                            >  SRR19973074.506452/1‑150 (MQ=60)
                                                                        GGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGC                                                                            >  SRR19973074.506264/1‑150 (MQ=60)
                                                                             GCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTG                                                                       >  SRR19973074.3455302/1‑150 (MQ=60)
                                                                               CTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAC                                                                     <  SRR19973074.1884728/150‑1 (MQ=60)
                                                                               CTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAC                                                                     <  SRR19973074.3480747/150‑1 (MQ=60)
                                                                               CTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGAC                                                                     <  SRR19973074.3512994/150‑1 (MQ=60)
                                                                                TGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACT                                                                    <  SRR19973074.801906/150‑1 (MQ=60)
                                                                                    GCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCT                                                                <  SRR19973074.230450/150‑1 (MQ=60)
                                                                                     CCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTG                                                               >  SRR19973074.1762198/1‑150 (MQ=60)
                                                                                       AGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAA                                                             >  SRR19973074.2357903/1‑150 (MQ=60)
                                                                                       AGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAA                                                             >  SRR19973074.849588/1‑150 (MQ=60)
                                                                                         ATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAC                                                           >  SRR19973074.1249382/1‑150 (MQ=60)
                                                                                         ATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAC                                                           >  SRR19973074.278621/1‑150 (MQ=60)
                                                                                         ATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAAC                                                           >  SRR19973074.1076789/1‑150 (MQ=60)
                                                                                          TAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACG                                                          <  SRR19973074.1465305/150‑1 (MQ=60)
                                                                                             TATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGC                                                       >  SRR19973074.2129049/1‑150 (MQ=60)
                                                                                              ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                      >  SRR19973074.532083/1‑150 (MQ=60)
                                                                                              ATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCG                                                      >  SRR19973074.2577783/1‑150 (MQ=60)
                                                                                                GTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGA                                                    >  SRR19973074.1118011/1‑150 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGGCTTTCTGAAACGCGCGGAAAAG                                                <  SRR19973074.2080737/150‑1 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAG                                                >  SRR19973074.3213814/1‑150 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAG                                                >  SRR19973074.3213778/1‑150 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAG                                                <  SRR19973074.2080755/150‑1 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAG                                                >  SRR19973074.3148610/1‑150 (MQ=60)
                                                                                                    AGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTGTCTCCTGCCAGCGTCTGTCTAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGACACGCGCGGAAAAG                                                >  SRR19973074.3148657/1‑150 (MQ=60)
                                                                                                      TGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGT                                              >  SRR19973074.1331101/1‑150 (MQ=60)
                                                                                                       GAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTC                                             >  SRR19973074.1308941/1‑150 (MQ=60)
                                                                                                       GAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTC                                             <  SRR19973074.2472499/150‑1 (MQ=60)
                                                                                                        AAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCA                                            <  SRR19973074.851916/150‑1 (MQ=60)
                                                                                                        AAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCA                                            <  SRR19973074.3123945/150‑1 (MQ=60)
                                                                                                        AAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCA                                            <  SRR19973074.1271651/150‑1 (MQ=60)
                                                                                                         AGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCAT                                           <  SRR19973074.2210731/150‑1 (MQ=60)
                                                                                                            GGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCC                                        <  SRR19973074.2031333/150‑1 (MQ=60)
                                                                                                            GGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCC                                        <  SRR19973074.2031313/150‑1 (MQ=60)
                                                                                                             GCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCC                                       <  SRR19973074.871494/150‑1 (MQ=60)
                                                                                                              CAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCT                                      <  SRR19973074.3058555/150‑1 (MQ=60)
                                                                                                              CAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCT                                      <  SRR19973074.3058554/150‑1 (MQ=60)
                                                                                                               AATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGTGTTTTCTCCTGCCAGCGTCTGTATTGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTA                                     <  SRR19973074.1012755/150‑1 (MQ=60)
                                                                                                               AATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTA                                     <  SRR19973074.1427563/150‑1 (MQ=60)
                                                                                                               AATGACGCGCGGTTTAAACTATCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTA                                     <  SRR19973074.547341/150‑1 (MQ=60)
                                                                                                                    CGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGA                                <  SRR19973074.1214945/150‑1 (MQ=60)
                                                                                                                      CGCGGTTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATT                              <  SRR19973074.3296618/150‑1 (MQ=60)
                                                                                                                      CGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATT                              <  SRR19973074.3230778/150‑1 (MQ=60)
                                                                                                                           TTTAAACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAAT                         <  SRR19973074.2033132/150‑1 (MQ=60)
                                                                                                                              AAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTAT                      >  SRR19973074.1624385/1‑150 (MQ=60)
                                                                                                                                ACTGTCTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTT                    <  SRR19973074.2698189/150‑1 (MQ=60)
                                                                                                                                    TCTGTCGCTGTTTGCCGAACGGATACTAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGG                <  SRR19973074.1217083/150‑1 (MQ=60)
                                                                                                                                     CTGTCGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGT               <  SRR19973074.4042147/150‑1 (MQ=60)
                                                                                                                                        TCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAAT            <  SRR19973074.2503172/150‑1 (MQ=60)
                                                                                                                                         CGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATG           <  SRR19973074.719729/150‑1 (MQ=60)
                                                                                                                                         CGCTGTTTGCCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGAGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATG           <  SRR19973074.1824709/150‑1 (MQ=60)
                                                                                                                                               TTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAG     >  SRR19973074.3451388/1‑150 (MQ=60)
                                                                                                                                                  CCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCCTACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGACATTATTTCCGGTAATGAAGAAGCCC  <  SRR19973074.2527891/150‑1 (MQ=60)
                                                                                                                                                  CCTAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC  <  SRR19973074.2527892/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CATGGTCATAGCCCGTGTGGTAGATGGTGCCATGCAGATTATTGGCCGCCTGAAACAGCGGGTGCATCTGGCGGACGGCCTGGGGCCAGATAATATGTTGAGTGAAGAGGCAATGACGCGCGGTTTAAACTGTCTGTCGCTGTTTGCCGAACGGCTACAAGGGTTTTCTCCTGCCAGCGTCTGTATAGTTGGTACCCATACGCTGCGTCAGGCGCTGAACGCCACTGACTTTCTGAAACGCGCGGAAAAGGTCATTCCCTACCCGATTGAAATTATTTCCGGTAATGAAGAAGCCC  >  CP009273/2618545‑2618840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑