Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,569,336 C→A 22.0% intergenic (‑172/+121) eutS ← / ← maeB putative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/putative phosphotransacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,569,3360CA22.0% 424.9 / 102.5 173intergenic (‑172/+121)eutS/maeBputative ethanol utilization carboxysome structural protein/malic enzyme: putative oxidoreductase/putative phosphotransacetylase
Reads supporting (aligned to +/- strand):  ref base C (71/64);  new base A (22/16);  total (93/80)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.77e-01

Only 100 of 175 total aligned reads displayed.

CGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCA  >  CP009273/2569189‑2569485
                                                                                                                                                   |                                                                                                                                                      
cGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCga                                                                                                                                                      <  2:4771786/150‑1 (MQ=255)
    tATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCACGCGAGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCTCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGatc                                                                                                                                                  >  1:1198729/1‑149 (MQ=255)
    tATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGata                                                                                                                                                  >  2:2861945/1‑150 (MQ=255)
        gTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGAtattat                                                                                                                                              <  2:3341922/150‑1 (MQ=255)
           aCAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATtatata                                                                                                                                           >  2:2101570/1‑150 (MQ=255)
            cAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAg                                                                                                                                          <  1:3341027/150‑1 (MQ=255)
             aaTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGa                                                                                                                                         <  2:4480464/150‑1 (MQ=255)
              aTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGAc                                                                                                                                        >  1:357174/1‑150 (MQ=255)
              aTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGAGATTATATAGAc                                                                                                                                        <  1:500968/150‑1 (MQ=255)
              aTTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGAc                                                                                                                                        >  2:2153566/1‑150 (MQ=255)
                ttttCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTg                                                                                                                                      <  2:3773827/150‑1 (MQ=255)
                    cGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttt                                                                                                                                  <  1:4175216/150‑1 (MQ=255)
                     gTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttt                                                                                                                                 >  2:2837687/1‑150 (MQ=255)
                      tCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttt                                                                                                                                <  1:2955188/150‑1 (MQ=255)
                       cAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttt                                                                                                                               <  1:4800868/150‑1 (MQ=255)
                        agagTAAATAACAAAATTCGGCACGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttttt                                                                                                                              >  1:3491814/1‑150 (MQ=255)
                        agagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCttttttt                                                                                                                              <  1:4229785/150‑1 (MQ=255)
                        agagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCttttttt                                                                                                                              >  1:801080/1‑150 (MQ=255)
                         gagTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCtttttttt                                                                                                                             <  2:743959/150‑1 (MQ=255)
                           gTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTttat                                                                                                                           <  2:2789183/150‑1 (MQ=255)
                               aTAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTattatt                                                                                                                       >  2:4233508/1‑150 (MQ=255)
                                   cAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCa                                                                                                                   <  1:2859103/150‑1 (MQ=255)
                                      aaTTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAAc                                                                                                                <  1:3123112/150‑1 (MQ=255)
                                       aggcGGCAAGCGCGTATAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACt                                                                                                               <  2:3742894/147‑1 (MQ=255)
                                         tCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCa                                                                                                             <  2:3531501/150‑1 (MQ=255)
                                           ggCACGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGt                                                                                                           >  1:2745902/1‑150 (MQ=255)
                                              aaGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATCTTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaat                                                                                                        >  2:2088481/1‑150 (MQ=255)
                                               aGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaata                                                                                                       <  2:3618463/150‑1 (MQ=255)
                                               aGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGtaata                                                                                                       >  1:4418727/1‑150 (MQ=255)
                                                 cgcgTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑g                                                                                                    <  1:2520606/150‑1 (MQ=255)
                                                  gcgTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑Gc                                                                                                   >  2:1325533/1‑150 (MQ=255)
                                                   cgTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCt                                                                                                  >  2:2240379/1‑150 (MQ=255)
                                                    gtatAAAAGGTGAGGTAGATAACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCtt                                                                                                 <  2:4566839/147‑1 (MQ=255)
                                                      ttAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTa                                                                                               >  2:1944467/1‑150 (MQ=255)
                                                      ttAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTa                                                                                               <  2:292596/150‑1 (MQ=255)
                                                      ttAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTa                                                                                               >  2:248965/1‑150 (MQ=255)
                                                       tAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTAc                                                                                              <  1:1989039/150‑1 (MQ=255)
                                                       tAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTAc                                                                                              <  1:700602/150‑1 (MQ=255)
                                                             gTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAatat                                                                                        <  2:1460760/150‑1 (MQ=255)
                                                             gTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAatat                                                                                        <  1:3657591/150‑1 (MQ=255)
                                                               gAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAatatat                                                                                      >  2:4492334/1‑150 (MQ=255)
                                                               gAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAatatat                                                                                      >  2:4859371/1‑150 (MQ=255)
                                                               gAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAatatat                                                                                      >  1:4003461/1‑150 (MQ=255)
                                                                aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATAtt                                                                                     >  2:3026212/1‑150 (MQ=255)
                                                                aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATAtt                                                                                     <  1:3089547/150‑1 (MQ=255)
                                                                aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATAtt                                                                                     <  1:4563554/150‑1 (MQ=255)
                                                                aGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATAtt                                                                                     <  1:4848388/150‑1 (MQ=255)
                                                                    aGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAg                                                                                 >  1:4554614/1‑150 (MQ=255)
                                                                     gATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGc                                                                                >  1:312890/1‑150 (MQ=255)
                                                                      aTCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCt                                                                               <  2:2735940/150‑1 (MQ=255)
                                                                      aTCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCt                                                                               <  2:1061860/150‑1 (MQ=255)
                                                                       tCACTAAGAATGGAGAGATATTCGCTTTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTa                                                                              <  2:2442007/150‑1 (MQ=255)
                                                                       tCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTa                                                                              <  2:4526024/150‑1 (MQ=255)
                                                                       tCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTa                                                                              <  2:2297449/150‑1 (MQ=255)
                                                                        cACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAAATCAGTAATAA‑GATTTACTAATATATTGCAGCTaa                                                                             >  1:2713334/1‑150 (MQ=255)
                                                                           tAAGAATGGAGAGATAGTCGCTGTGGTTCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAT‑GCTTTACTAATATATTGCAGCTAAACt                                                                          <  2:2584912/150‑1 (MQ=255)
                                                                             aGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGc                                                                        >  1:1748863/1‑150 (MQ=255)
                                                                              gAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCAAATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCt                                                                       >  1:1475255/1‑150 (MQ=255)
                                                                               aaTGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCtt                                                                      <  2:3892311/150‑1 (MQ=255)
                                                                                 tGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTAATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTAc                                                                    >  1:3986172/1‑150 (MQ=255)
                                                                                    agagaTATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCt                                                                 >  2:3096192/1‑150 (MQ=255)
                                                                                    agagaTATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCt                                                                 >  2:1418290/1‑150 (MQ=255)
                                                                                        atatTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAat                                                             >  1:3364814/1‑150 (MQ=255)
                                                                                         tatTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAata                                                            <  2:643831/150‑1 (MQ=255)
                                                                                         tatTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAata                                                            <  2:644286/150‑1 (MQ=255)
                                                                                             cGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCa                                                        >  2:4281291/1‑150 (MQ=255)
                                                                                              gCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAg                                                       <  2:402675/150‑1 (MQ=255)
                                                                                                tgtgGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAggg                                                     >  2:1137426/1‑150 (MQ=255)
                                                                                                 gtggtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGt                                                    >  2:2899938/1‑150 (MQ=255)
                                                                                                  tggtgCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTa                                                   <  2:801196/150‑1 (MQ=255)
                                                                                                       cATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGt                                              <  1:1001172/150‑1 (MQ=255)
                                                                                                          aaaCTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTgag                                           <  2:4636577/150‑1 (MQ=255)
                                                                                                          aaaCTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTgag                                           >  2:3658618/1‑150 (MQ=255)
                                                                                                              tttCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGtt                                       >  1:3770084/1‑150 (MQ=255)
                                                                                                                    atGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaaa                                 <  1:4440354/150‑1 (MQ=255)
                                                                                                                    atGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaaa                                 <  1:4657443/150‑1 (MQ=255)
                                                                                                                    atGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GATTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaaa                                 >  1:3878336/1‑150 (MQ=255)
                                                                                                                     tGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTCAGCGTGAGAGTTaaaaaaa                                >  2:3441417/1‑150 (MQ=255)
                                                                                                                     tGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaaaa                                >  2:3263357/1‑150 (MQ=255)
                                                                                                                     tGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTaaaaaaa                                >  2:3263369/1‑150 (MQ=255)
                                                                                                                        aaCGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAAtt                             >  2:1305355/1‑150 (MQ=255)
                                                                                                                            cATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAg                         >  2:4739271/1‑150 (MQ=255)
                                                                                                                            cATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTTAGCGTGAGAGTTAAAAAAAATTACAg                         >  2:3636326/1‑150 (MQ=255)
                                                                                                                            cATGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAg                         >  1:2232264/1‑150 (MQ=255)
                                                                                                                             aTGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGc                        >  2:4332279/1‑150 (MQ=255)
                                                                                                                             aTGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGc                        >  2:1072649/1‑150 (MQ=255)
                                                                                                                             aTGAATATAAATAAAATAATATAGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGc                        >  1:4261250/1‑150 (MQ=255)
                                                                                                                               gAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCgg                      >  1:1441429/1‑150 (MQ=255)
                                                                                                                                 atatAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGtt                    <  1:2837687/150‑1 (MQ=255)
                                                                                                                                      aataaaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGtt               >  2:154378/1‑150 (MQ=255)
                                                                                                                                      aataaaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGtt               >  2:4812701/1‑150 (MQ=255)
                                                                                                                                      aataaaataaTATCGAGATATTATATAGACTGCTTTTTTTTAATTTTTCCAAAAGAAATAAAAATGCTTTAATAATTTATTGCAGCTAAAATGATTACCCGGATTTTTCAGGTTAAGGTTGGGAGTTAAAAAAATTTACAGCGGTTGGGt                >  2:154521/1‑150 (MQ=255)
                                                                                                                                        taaaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTg             >  1:2725239/1‑150 (MQ=255)
                                                                                                                                        taaaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTg             >  2:1357226/1‑150 (MQ=255)
                                                                                                                                         aaaataaTATCGAGATATTATATAGACTGCTTTTTATTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTgc            <  2:2936079/150‑1 (MQ=255)
                                                                                                                                          aaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTgcg           >  1:175507/1‑150 (MQ=255)
                                                                                                                                          aaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTgcg           >  1:1101081/1‑150 (MQ=255)
                                                                                                                                          aaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTgcg           >  1:1042514/1‑150 (MQ=255)
                                                                                                                                          aaataaTATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGGTGGGTTTgcg           >  1:2604758/1‑150 (MQ=255)
                                                                                                                                                 atCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTAc    <  1:2856114/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                      
CGTGTATCGTGACAATTTTTCGTCAGAGTAAATAACAAAATTCGGCAAGCGCGTTTAAAAGGTGAGGTAGATCACTAAGAATGGAGAGATATTCGCTGTGGTGCATAAACTTTCATATGCAACGCATGAATATAAATAAAATAATATCGAGATATTATATAGACTGCTTTTTTTTATTATTCGCAAACTCAGTAATAA‑GCTTTACTAATATATTGCAGCTAAACTGCTTACCCTGAATATTCAGGGTAAGCGTGAGAGTTAAAAAAAATTACAGCGGTTGGGTTTGCGCTTCTACCA  >  CP009273/2569189‑2569485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A