Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F1 I1 R1 255 512.9 22377240 99.2% 22198222 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1620NC99.8% 1582.1 / NA 430intergenic (+23/‑38)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (196/232);  minor base A (1/0);  total (197/233)
Fisher's exact test for biased strand distribution p-value = 4.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 431 total aligned reads displayed.

GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310
                                                                                                                                                    |                                                                                                                                                     
ggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACATCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGGTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                      >  2:2117006/1‑150 (MQ=255)
 gggAGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCACCTACACCAACTTCATCGACGGCAAGGACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCgg                                                                                                                                                     <  1:9460787/150‑1 (MQ=255)
  ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCggg                                                                                                                                                    <  1:1317478/150‑2 (MQ=255)
  ggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCggg                                                                                                                                                    >  2:2164280/1‑149 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                   <  1:7486798/150‑1 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCGTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                   <  2:6366901/150‑1 (MQ=255)
    tGGACGCGGAGTACCAGAACACCTACACCGCGAGGCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGTAAGTCTGATGTACGCCGGCCCCGTGCGAACCGGGcc                                                                                                                                                  >  1:10252529/1‑150 (MQ=255)
        cgcgGAGTACCAGAACACCTACCCCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCg                                                                                                                                              <  2:9390895/150‑1 (MQ=255)
           ggAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                           >  2:2668170/1‑150 (MQ=255)
             aGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAg                                                                                                                                         >  2:8139842/1‑150 (MQ=255)
                aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                                      >  1:9822917/1‑150 (MQ=255)
                  cAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACATCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                    >  1:7549066/1‑150 (MQ=255)
                   aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTa                                                                                                                                   <  2:5022814/150‑1 (MQ=255)
                    gAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCCGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGCGCAGGACTTaa                                                                                                                                  >  1:10796050/1‑150 (MQ=255)
                      acacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACg                                                                                                                                <  1:595410/150‑1 (MQ=255)
                       cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                               >  2:3463265/1‑150 (MQ=255)
                        acCTCCACCGCGAGCCTCTCCTACACCAACTTCTTCGACGTCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                              <  2:4259032/150‑1 (MQ=255)
                        acCTACACCGCGAGCCTCTCCTACCCCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                              <  1:1825526/150‑1 (MQ=255)
                        acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                              >  2:9881737/1‑150 (MQ=255)
                        acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATAGTCGCGCTCAGCTTCGGCATGAACTTATGTTGTACGCCCGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAAagag                                                                                                                              >  1:10387998/1‑150 (MQ=255)
                          cTACACCGCGAGCCTCTCCTACACCACCTTCTTCGACGGCAAGTCCACCACCGTGTACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                            <  2:4764560/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAATTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           <  2:287758/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           <  1:1536857/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCGCAGATTCGGCATGAACTTCTGATGTACTCCGGCCCGGGGCGAACAGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                           >  2:10130040/1‑150 (MQ=255)
                            ccacCGCGAGCCTCTCCAACACCAACTTCTTCGACGGCAAGTACCCCCCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacg                                                                                                                          <  1:7963726/149‑1 (MQ=255)
                              acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACTATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTACTGATGTACGCCGGCCCGGTGAGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                        >  2:10894133/1‑150 (MQ=255)
                              acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGCACCGGGCCGGCGAGCCGGACTTAACGAGgacgac                                                                                                                        >  1:6309432/1‑150 (MQ=255)
                              acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                        <  1:7073634/150‑1 (MQ=255)
                              acCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacgac                                                                                                                        <  1:5404483/150‑1 (MQ=255)
                               ccGCGAGCCTCTCCTACACCAACTTCTTCGCCGGCAAGTACACCTCCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                       <  2:835510/150‑1 (MQ=255)
                               ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGacgacg                                                                                                                       >  1:6344743/1‑150 (MQ=255)
                               ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTAGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAACGAGGcagacg                                                                                                                       >  2:410672/1‑150 (MQ=255)
                                 gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACGTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                     <  2:7412575/150‑1 (MQ=255)
                                  cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                    <  1:11092562/150‑1 (MQ=255)
                                  cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTAGGCATGAACGTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACGTAAAGAGGACGACGAGc                                                                                                                    >  1:65164/1‑150 (MQ=255)
                                    aGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGa                                                                                                                  <  2:9191461/150‑1 (MQ=255)
                                       ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                               <  1:8062202/150‑1 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑a                                                                                                             >  2:5161827/1‑150 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACGTAGTCGCGCTCAGCTTCGGCCTGTACATCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGAATG‑a                                                                                                             >  2:7833668/1‑150 (MQ=255)
                                          accTACACCAACTTCTTCGACGGAAAGAACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑aaa                                                                                                           <  1:9288284/149‑1 (MQ=255)
                                           ccTCCACCCACTCCCGCGACGGCAAGTACACCACCGCGGCCGAACGCGACTCCGTCGCGCTCAGCTTCGGCATGAACATCTGAAGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑aaaa                                                                                                          <  2:6005916/150‑1 (MQ=255)
                                           ccTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑aaaa                                                                                                          >  2:3531752/1‑150 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaac                                                                                                         <  1:10667106/150‑1 (MQ=255)
                                            cTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAaac                                                                                                         >  2:4873305/1‑150 (MQ=255)
                                                 ccACCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGGGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGa                                                                                                    <  1:5178231/150‑1 (MQ=255)
                                                 ccAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGa                                                                                                    >  1:9170290/1‑150 (MQ=255)
                                                  cAACTTCTTCGACGGCAAGTACACCACTGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGTCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGaa                                                                                                   <  1:2721070/150‑1 (MQ=255)
                                                  cAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGaa                                                                                                   <  2:8986285/150‑1 (MQ=255)
                                                  cAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGaa                                                                                                   <  1:5009728/150‑1 (MQ=255)
                                                  cAACTTCTACGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGaa                                                                                                   <  1:2097356/150‑1 (MQ=255)
                                                     cttcttCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAt                                                                                                <  1:3446666/150‑1 (MQ=255)
                                                     cttcttCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCGCAGCTTCGGCATGTAGTTGTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAAAGAGGACGACGAGCGTATG‑AAAACAACGAAGAt                                                                                                >  1:46010/1‑150 (MQ=255)
                                                      ttcttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAtt                                                                                               <  2:6842891/150‑1 (MQ=255)
                                                         ttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAttctt                                                                                            <  2:7983049/150‑1 (MQ=255)
                                                          tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTG‑AAAACAACGAAGAttcttc                                                                                           >  2:1393736/1‑150 (MQ=255)
                                                          tcGACGGCAAGTACACCACCGGGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGAttcttc                                                                                           <  1:3520861/150‑1 (MQ=255)
                                                            gACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGCCTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTg                                                                                         >  2:7567668/1‑150 (MQ=255)
                                                             aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGAGGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGc                                                                                        <  2:228046/150‑1 (MQ=255)
                                                              cGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCGGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAATCTTCTTCTGCa                                                                                       >  2:4485311/1‑150 (MQ=255)
                                                              cGGCAAGTACACCACCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCTGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGCAAG‑AAAACAACGAAGAGTCTTCTGCa                                                                                       >  2:3081711/1‑150 (MQ=255)
                                                                gCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑GAAAAAACGAAGATTCTTCTGCATa                                                                                     >  2:67647/1‑150 (MQ=255)
                                                                gCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATa                                                                                     <  2:9089927/150‑1 (MQ=255)
                                                                 cAAGTCCACCACCGTGGTCGATCGCGAATTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTTACGATGACGACGAGCGACTG‑AAAACAACGAAGATTCTTCTGCAttc                                                                                    <  2:616630/150‑3 (MQ=255)
                                                                  aaGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATAcc                                                                                   <  1:10011253/150‑1 (MQ=255)
                                                                   aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTGTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGCCGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCg                                                                                  >  1:8827355/1‑150 (MQ=255)
                                                                   aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCg                                                                                  >  2:7135881/1‑150 (MQ=255)
                                                                    gTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCgg                                                                                 <  1:4674182/150‑1 (MQ=255)
                                                                           accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                          <  2:2934318/150‑1 (MQ=255)
                                                                               tGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCCGCGAGCAGGACTTAAAGAGGACGACGAGCGAAAG‑AAAACAACGAAGATTCTTATGCATACCGGTGTAATGGccc                                                                      >  2:6977761/1‑150 (MQ=255)
                                                                                ggACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTAATGGCCCt                                                                     >  1:10638591/1‑150 (MQ=255)
                                                                                     aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                                >  2:10495939/1‑150 (MQ=255)
                                                                                     aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                                <  2:2570427/150‑1 (MQ=255)
                                                                                     aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                                <  1:10129403/150‑1 (MQ=255)
                                                                                     aTCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGcc                                                                >  2:11097203/1‑150 (MQ=255)
                                                                                        gcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑CAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgc                                                             >  1:2433626/1‑150 (MQ=255)
                                                                                         cgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgct                                                            >  2:469307/1‑150 (MQ=255)
                                                                                          gACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgctg                                                           <  2:8617275/150‑1 (MQ=255)
                                                                                            cTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTgg                                                          >  1:1203463/1‑150 (MQ=255)
                                                                                                     gcTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGt                                                <  1:4222120/150‑1 (MQ=255)
                                                                                                      cTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTg                                               >  2:1892663/1‑150 (MQ=255)
                                                                                                        cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                             >  2:6681038/1‑150 (MQ=255)
                                                                                                        cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                             >  1:7708149/1‑150 (MQ=255)
                                                                                                                gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGcggcgg                                     <  2:8205606/150‑1 (MQ=255)
                                                                                                                 cATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGt                                    >  1:3730576/1‑150 (MQ=255)
                                                                                                                     aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                                <  2:1000596/150‑1 (MQ=255)
                                                                                                                     aaCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATcc                                >  1:861111/1‑150 (MQ=255)
                                                                                                                          cTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcga                           <  2:3943798/150‑1 (MQ=255)
                                                                                                                          cTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcga                           <  1:8383994/150‑1 (MQ=255)
                                                                                                                          cTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGGGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcga                           <  1:4922450/150‑1 (MQ=255)
                                                                                                                          cTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCGTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcga                           <  2:8680241/150‑1 (MQ=255)
                                                                                                                               gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAgg                      <  2:7811682/150‑1 (MQ=255)
                                                                                                                                caCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc                     <  1:2865039/149‑1 (MQ=255)
                                                                                                                                   cccGGCCCGGTGCGCACCGGGCCGGCGAGCACGACAGAACGAGGACGACGAGCGAAAG‑ACAACAACGAAGATTCTTCGGCATACCGGAGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcgcc                  <  1:3125685/149‑3 (MQ=255)
                                                                                                                                       gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  1:4497635/1‑150 (MQ=255)
                                                                                                                                       gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  2:9554130/1‑150 (MQ=255)
                                                                                                                                        cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc             >  2:5638179/1‑150 (MQ=255)
                                                                                                                                         ccGGTGCTCACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGGACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt            <  1:6781327/150‑1 (MQ=255)
                                                                                                                                         ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt            <  1:2541646/150‑1 (MQ=255)
                                                                                                                                          cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc           <  1:3342938/150‑1 (MQ=255)
                                                                                                                                           ggTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCg          >  2:5594407/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATG‑AAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A