Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 255 512.9 22386308 99.2% 22207217 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_008463 1,128,162 N→C intergenic (+23/‑38) PA14_RS05280 → / → PA14_RS05285 DUF1302 domain‑containing protein/DUF1329 domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0084631,128,1620NC99.8% 1540.9 / ‑4.4 412intergenic (+23/‑38)PA14_RS05280/PA14_RS05285DUF1302 domain‑containing protein/DUF1329 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base N (0/0);  major base C (201/210);  minor base A (0/1);  total (201/211)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01

Only 100 of 418 total aligned reads displayed.

GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310
                                                                                                                                                    |                                                                                                                                                    
ggggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCg                                                                                                                                                     <  2:5273500/150‑1 (MQ=255)
 gggTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCgg                                                                                                                                                    <  1:7339612/150‑1 (MQ=255)
   gTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGc                                                                                                                                                  <  2:4078774/150‑1 (MQ=255)
     ggACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCg                                                                                                                                                <  2:4876799/150‑1 (MQ=255)
      gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCTACGGCAAGTACACCCCCGTGGACGAGCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                               >  2:10330073/1‑150 (MQ=255)
      gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCgg                                                                                                                                               <  2:1363129/150‑1 (MQ=255)
      gACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCgg                                                                                                                                               <  2:8735078/150‑1 (MQ=255)
       aCGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGc                                                                                                                                              <  1:10990190/150‑1 (MQ=255)
          cgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAg                                                                                                                                           >  2:6232875/1‑150 (MQ=255)
          cgGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAg                                                                                                                                           >  1:7137902/1‑150 (MQ=255)
           ggAGTACCAGAACACCTACACCGCGCGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGc                                                                                                                                          <  2:9088127/150‑1 (MQ=255)
            gAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCa                                                                                                                                         <  1:4477954/150‑1 (MQ=255)
                aCCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGAc                                                                                                                                     >  2:9256488/1‑150 (MQ=255)
                  cAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACtt                                                                                                                                   <  1:5349199/150‑1 (MQ=255)
                   aGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCCGTGCGAACCCGGCCGGCGAGCCGGACttt                                                                                                                                  >  1:9328927/1‑149 (MQ=255)
                       ccccTACCCCGCGAGCCTCACCTCCACCAACTTCTTCGACGGCAAGTCCACCACGGTGGACGATCGCGACTTCGTCGCGCTCTGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                              <  1:6544120/148‑1 (MQ=255)
                       cacCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGa                                                                                                                              >  1:8344013/1‑150 (MQ=255)
                        acCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACCCCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAg                                                                                                                             <  1:1569417/150‑1 (MQ=255)
                          cTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                           <  1:8714547/150‑1 (MQ=255)
                          cTACACCGCGAGCCTCTCCTACACCAACTCCTTCGTCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGTTGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGga                                                                                                                           <  1:379412/150‑1 (MQ=255)
                           tACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgac                                                                                                                          <  2:10981685/150‑1 (MQ=255)
                            acacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCCGCCCCGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGgacg                                                                                                                         >  1:1923848/1‑150 (MQ=255)
                             cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCGGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacga                                                                                                                        <  1:10805156/150‑1 (MQ=255)
                             cacCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGgacga                                                                                                                        >  1:4324173/1‑150 (MQ=255)
                               ccGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCCGGACTTAAAGAGGacgacg                                                                                                                      >  1:1302050/1‑150 (MQ=255)
                                 gcgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAg                                                                                                                    <  2:8510607/150‑1 (MQ=255)
                                  cgAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGc                                                                                                                   <  2:3659493/150‑1 (MQ=255)
                                   gAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCg                                                                                                                  >  2:5570376/1‑150 (MQ=255)
                                      ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                               <  1:9584945/150‑1 (MQ=255)
                                      ccTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAt                                                                                                               >  1:3559796/1‑150 (MQ=255)
                                       ctctCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATg                                                                                                              >  2:7771922/1‑150 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                             <  1:470393/150‑1 (MQ=255)
                                        tctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGa                                                                                                             >  1:10014497/1‑150 (MQ=255)
                                         ctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGaa                                                                                                            <  1:3216577/150‑1 (MQ=255)
                                         ctcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGTGCAGGACTTAACGAGGACGACGAGCGAATGaa                                                                                                            <  2:3583094/150‑1 (MQ=255)
                                          tcCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGAGCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCCGCCCGGTGCGAACCGGGCCGGCGCGCAGGACTTAACGAGGACGACGAGCGAATGaaa                                                                                                           >  1:3281621/1‑150 (MQ=255)
                                            cgacacCCACTTCTCCGCCGCCCAGCACACCACCGTGGACGCTCGCGACTCCGCCGCGCTCAGCTTCGGCATGAACTTCTGCGCTACGCCGGCCCGGGGCGAACCGGGCCGGCGAGCAGGACTTCACGAGGACGACGAGCGAATGACaac                                                                                                         <  2:7653576/148‑1 (MQ=255)
                                                 ccAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGa                                                                                                    <  2:1537212/150‑1 (MQ=255)
                                                   aaCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTTCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAg                                                                                                  >  1:6940387/1‑150 (MQ=255)
                                                    aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGa                                                                                                 <  2:3326266/150‑1 (MQ=255)
                                                    aCTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGa                                                                                                 <  1:8399681/150‑1 (MQ=255)
                                                     cttcttCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAAACGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGACTGAAAACAACGAAGAt                                                                                                >  2:8110416/1‑150 (MQ=255)
                                                        cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTGGGCATGAACTTCTGATGTTCGCCGGCCCGGTGGGAACCGGGCCGGCGAGCAGGTCTTAACGAGGACGACGAGCGAATGAAAACAACGCAGAttgt                                                                                             >  1:6682737/1‑148 (MQ=255)
                                                        cttcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttct                                                                                             <  1:4110930/150‑1 (MQ=255)
                                                          tcGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGAttcttc                                                                                           <  1:1226920/150‑1 (MQ=255)
                                                           cGACGGCAAGTACCCCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGTGGACGACGAGCGAATGAAAACAACGAAGATtcgtct                                                                                          >  2:3788193/1‑150 (MQ=255)
                                                           cGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGTGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATtcatct                                                                                          >  2:10120843/1‑150 (MQ=255)
                                                             aCGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGc                                                                                        <  1:9232242/150‑1 (MQ=255)
                                                              cGGCAAGTACACCACCGTGGACGATGGCGACTTCGTCGCGCTCAGCTTCGGCATGAAATTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGCGCAGGACGTAACGAGGACGCCGGTCGAATTTAAACAACGTAGAttcttaggca                                                                                       >  1:7644670/1‑145 (MQ=255)
                                                               ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                      <  2:102933/150‑1 (MQ=255)
                                                               ggCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCAt                                                                                      <  1:3409871/150‑1 (MQ=255)
                                                               ggCAAGTACACCACCGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGCCTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCaa                                                                                      >  1:305128/1‑149 (MQ=255)
                                                                 cAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAc                                                                                    <  1:3099794/150‑1 (MQ=255)
                                                                   aGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACATCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCATGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATAACg                                                                                  >  2:7593473/1‑150 (MQ=255)
                                                                        accaccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCCTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTa                                                                             >  2:7024876/1‑150 (MQ=255)
                                                                          caccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGTCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCTAATGAAAACAACGAAGATTCTTCTGCTTACCGGTGTACt                                                                           <  2:9716506/150‑1 (MQ=255)
                                                                          caccGTGGACGATCGCGACTTAGTCGCGCTCAGCTTAGGCATGAAATTCAGATGTACGCCGGCCCGGTGCGAAACGGGCCGGCGAGCCGGACTTAACGAGGACTACGAGCGAATGAAAACAACGAAGATTAATCTGCATAACGGTGTACt                                                                           >  2:1156788/1‑150 (MQ=255)
                                                                           accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                          >  2:7175296/1‑150 (MQ=255)
                                                                           accGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCAAGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTg                                                                          >  2:11112240/1‑150 (MQ=255)
                                                                           accGTGGACGATCGCGACATCGTCGCGCTCAGCTTCGGCATGAACATCTGATGTAAGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAAAGAAAACAACGAAGATTCTTATGCATACCGGTGTACTg                                                                          >  1:2674360/1‑150 (MQ=255)
                                                                            ccGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATTAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTgg                                                                         <  1:5037494/150‑1 (MQ=255)
                                                                            ccGTGGACGATCGCGACTACGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTgg                                                                         <  2:1443658/150‑1 (MQ=255)
                                                                             cGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTATGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACATAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGc                                                                        >  2:6998177/1‑150 (MQ=255)
                                                                              gTGGACGATCGCGACCTCGTCGCGCTCAGCTTCGGCAAGAACTTCCGATGGACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGCGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGcc                                                                       <  2:4612154/150‑1 (MQ=255)
                                                                                      tCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCct                                                               <  1:6226374/150‑1 (MQ=255)
                                                                                       cgcgACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctg                                                              >  2:2760675/1‑150 (MQ=255)
                                                                                          gACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCctgctg                                                           >  2:2465893/1‑150 (MQ=255)
                                                                                               cgtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAc                                                      <  2:2244796/150‑1 (MQ=255)
                                                                                                gtcgCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCAcc                                                     >  1:9825331/1‑150 (MQ=255)
                                                                                                  cgcgCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCa                                                   <  2:4307985/150‑1 (MQ=255)
                                                                                                        cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                             <  1:8153452/150‑1 (MQ=255)
                                                                                                        cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGAt                                             >  1:10679178/1‑150 (MQ=255)
                                                                                                        cAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTATGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTgtt                                             <  2:10068524/150‑3 (MQ=255)
                                                                                                           cTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggc                                          <  1:10246361/150‑1 (MQ=255)
                                                                                                            ttCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                         >  1:9027567/1‑150 (MQ=255)
                                                                                                            ttCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcg                                         >  1:10168226/1‑150 (MQ=255)
                                                                                                             tCGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATggcgg                                        <  2:6119316/150‑1 (MQ=255)
                                                                                                              cGGCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCGTCGGCATACCGGTGTACTGGCCATGAGCCTGCTGGCCACCCAGGTGATggcggc                                       <  1:368504/150‑1 (MQ=255)
                                                                                                                gCATGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGcggcgg                                     >  2:4255667/1‑150 (MQ=255)
                                                                                                                  aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                                   >  1:10454544/1‑150 (MQ=255)
                                                                                                                  aTGAACTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTa                                   >  2:6719656/1‑150 (MQ=255)
                                                                                                                      aCTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccg                               <  2:10603364/150‑1 (MQ=255)
                                                                                                                       cTTCTGATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATccgc                              >  2:6159749/1‑150 (MQ=255)
                                                                                                                            gATGTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCcgacg                         >  2:2040700/1‑150 (MQ=255)
                                                                                                                               gTACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAgg                      >  1:5685891/1‑150 (MQ=255)
                                                                                                                                tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc                     <  2:5108839/150‑1 (MQ=255)
                                                                                                                                tACGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggc                     <  2:8836918/150‑1 (MQ=255)
                                                                                                                                 aCGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcg                    >  2:5405279/1‑150 (MQ=255)
                                                                                                                                  cGCCGGCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcgg                   >  2:1849126/1‑150 (MQ=255)
                                                                                                                                   gCCGGCCCGGTGCGACCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAggcggc                  >  2:5715057/1‑150 (MQ=255)
                                                                                                                                       gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  2:5955139/1‑150 (MQ=255)
                                                                                                                                       gCCCGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAg              >  2:7270547/1‑150 (MQ=255)
                                                                                                                                        cccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGc             >  1:2161555/1‑150 (MQ=255)
                                                                                                                                         ccGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCt            >  1:6436084/1‑150 (MQ=255)
                                                                                                                                          cGGTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTc           >  2:2415407/1‑150 (MQ=255)
                                                                                                                                            gTGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCgg         <  1:6024093/150‑1 (MQ=255)
                                                                                                                                             tGCGAACCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGc        >  1:7788078/1‑150 (MQ=255)
                                                                                                                                                gaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCAcc     >  1:1719630/2‑150 (MQ=255)
                                                                                                                                                gaaCCGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGGGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCAcc     <  2:160414/149‑1 (MQ=255)
                                                                                                                                                   ccGGGCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGc  <  2:9592959/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGGGTGGACGCGGAGTACCAGAACACCTACACCGCGAGCCTCTCCTACACCAACTTCTTCGACGGCAAGTACACCACCGTGGACGATCGCGACTTCGTCGCGCTCAGCTTCGGCATGAACTTCTGATGTACGCCGGCCCGGTNNNANCNGGNCCGGCGAGCAGGACTTAACGAGGACGACGAGCGAATGAAAACAACGAAGATTCTTCTGCATACCGGTGTACTGGCCCTGAGCCTGCTGGCCACCCAGGTGATGGCGGCGGTATCCGCCGACGAGGCGGCCAAGCTCGGCACCAGC  >  NC_008463/1128014‑1128310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A