Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R2 44 314.5 5848508 99.3% 5807568 128.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AssemblyC74_contig_12 59,062 T→C G92G (GGA→GGG)  rasttk_feature_annotation_tool=annotate_proteins_km er_v2 ← Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AssemblyC74_contig_1259,0620TC76.5% 2485.6 / inf 1192G92G (GGA→GGG) rasttk_feature_annotation_tool=annotate_proteins_km er_v2Putative peptidoglycan bound protein (LPXTG motif) Lmo2178 homolog
Reads supporting (aligned to +/- strand):  ref base T (107/170);  new base C (425/478);  total (536/656)
Fisher's exact test for biased strand distribution p-value = 1.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

Only 100 of 1198 total aligned reads displayed.

TCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGT  >  AssemblyC74_contig_12/58931‑59196
                                                                                                                                   |                                                                                                                                      
      agctgatacactcctggtgctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTgg                                                                                                                               >  2:1652967‑M1/31‑135 (MQ=255)
           atatGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGc                                                                                                                                    >  1:247207/1‑125 (MQ=255)
                 tccgggtgctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGGAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCtt                                                                                                                    >  2:1119010‑M1/20‑135 (MQ=255)
                  ccTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCtt                                                                                                                    <  1:724454/134‑1 (MQ=255)
                     ggtgctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTc                                                                                                                <  2:2470280‑M1/120‑1 (MQ=255)
                      gtgctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTc                                                                                                                <  2:2680440‑M1/120‑1 (MQ=255)
                      gtgctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCa                                                                                                               >  1:435222‑M1/15‑135 (MQ=255)
                        gctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCaa                                                                                                              <  1:2585011‑M1/122‑1 (MQ=255)
                        gctaagccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCATTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCaa                                                                                                              <  2:801609‑M1/122‑1 (MQ=255)
                        gCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCaa                                                                                                              >  1:2435818/1‑134 (MQ=255)
                           aaGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACg                                                                                                          <  2:61854/135‑1 (MQ=255)
                              ccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAacac                                                                                                           <  1:2147331‑M1/125‑1 (MQ=255)
                              ccgtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGac                                                                                                        <  2:2743263‑M1/128‑1 (MQ=255)
                                gtcaATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAcc                                                                                                     >  2:1801765‑M1/5‑135 (MQ=255)
                                    ATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGGTCTTTGTCAACACGACACCGCCt                                                                                                 <  1:1312082‑M1/135‑1 (MQ=255)
                                    ATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCt                                                                                                 <  2:2175947‑M1/135‑1 (MQ=255)
                                          AGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTa                                                                                           >  2:1191354‑M1/1‑135 (MQ=255)
                                           GTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTaa                                                                                          <  1:2641413‑M1/135‑1 (MQ=255)
                                                      GCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATtttct                                                                               <  1:1842272‑M1/135‑1 (MQ=255)
                                                       CTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTtttctt                                                                              >  2:2769587‑M1/1‑135 (MQ=255)
                                                         cgtgtgGTTAGTCCTGACTGCATTACGGTTGGATTGGGATCTTGTATCTGAATTACATCTCCTTGCAGGAGTTGTCCGATTTGGTCATCGATCTTTGTAATCACGTCACCGGCTGGTTTAAGTCGATtttctttc                                                                            <  2:885334/133‑1 (MQ=255)
                                                         CCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATtttctttc                                                                            >  2:2413832‑M1/1‑135 (MQ=255)
                                                         CCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATtttctttc                                                                            <  2:1755610‑M1/135‑1 (MQ=255)
                                                            GTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTc                                                                         >  1:2084853‑M1/1‑135 (MQ=255)
                                                               gttagaccggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCaa                                                                       >  2:1419957‑M1/66‑134 (MQ=255)
                                                                ttagaccggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCaa                                                                       <  1:1509550‑M1/69‑1 (MQ=255)
                                                                  AGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTc                                                                    >  2:847580‑M1/1‑134 (MQ=255)
                                                                      cggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTacca                                                               >  2:1320656‑M1/59‑135 (MQ=255)
                                                                       ggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAcc                                                                                                     >  2:750490‑M1/58‑96 (MQ=255)
                                                                       ggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAcc                                                                                                     <  2:1274357‑M1/39‑1 (MQ=255)
                                                                       ggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTaccac                                                              <  1:1004693‑M1/78‑1 (MQ=255)
                                                                       ggtttgtagcgtttctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTacca                                                               >  1:552271‑M1/58‑134 (MQ=255)
                                                                       TGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTaccac                                                              <  2:1947015‑M1/135‑1 (MQ=255)
                                                                            GCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACAcc                                                                                                     <  2:2360451‑M1/91‑1 (MQ=255)
                                                                            GCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTaccac                                                              <  1:512001‑M1/130‑1 (MQ=255)
                                                                            GCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTaccac                                                              <  1:154346‑M1/130‑1 (MQ=255)
                                                                            GCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTaccac                                                              <  1:118974‑M1/130‑1 (MQ=255)
                                                                            GCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTaccac                                                              <  1:988992‑M1/130‑1 (MQ=255)
                                                                                  CGGTTCAATTGGCATCTTGTAACTCAAACACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCt                                                      >  2:2539275‑M1/1‑132 (MQ=255)
                                                                                     ctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAcc                                                                                                     >  1:1036396‑M1/44‑82 (MQ=255)
                                                                                     ctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGt                                                                          >  1:407124‑M1/44‑109 (MQ=255)
                                                                                     ctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGActtc                                                <  1:1613742‑M1/92‑1 (MQ=255)
                                                                                     ctccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGActtc                                                <  1:1411315‑M1/92‑1 (MQ=255)
                                                                                      tccttctcggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGActtct                                               >  1:2386410‑M1/43‑135 (MQ=255)
                                                                                        CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGacac                                                                                                      <  2:1050173‑M1/78‑1 (MQ=255)
                                                                                        CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCttt                                             <  1:598367‑M1/135‑1 (MQ=255)
                                                                                        CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCttt                                             <  2:348325‑M1/135‑1 (MQ=255)
                                                                                        CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGt                                                                                                                              >  1:2891807‑M1/1‑54 (MQ=255)
                                                                                        CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCttt                                             <  1:2833379‑M1/135‑1 (MQ=255)
                                                                                             cggttctgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGAtc                                        >  1:1732150‑M1/36‑135 (MQ=255)
                                                                                                  TTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCa                                     <  2:920462‑M1/133‑1 (MQ=255)
                                                                                                  TTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCa                                     <  1:1891535‑M1/133‑1 (MQ=255)
                                                                                                  TTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCa                                     <  2:578233‑M1/133‑1 (MQ=255)
                                                                                                   tgtaactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCaaa                                   <  2:2823818‑M1/105‑1 (MQ=255)
                                                                                                    gTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTc                                <  1:678309/135‑1 (MQ=255)
                                                                                                     taactcaaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTc                                >  1:1360939‑M1/28‑135 (MQ=255)
                                                                                                     TAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTc                                >  2:1459952‑M1/1‑135 (MQ=255)
                                                                                                     TAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTc                                >  2:212460‑M1/1‑135 (MQ=255)
                                                                                                     TAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTc                                >  2:1836077‑M1/1‑135 (MQ=255)
                                                                                                       ACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGa                              >  2:189252‑M1/1‑135 (MQ=255)
                                                                                                          CAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTg                           <  2:1550397‑M1/135‑1 (MQ=255)
                                                                                                           aaaaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTgg                          >  2:287125‑M1/22‑135 (MQ=255)
                                                                                                            AAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGgt                         >  1:906123‑M1/1‑135 (MQ=255)
                                                                                                             aaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCaa                                                                       <  2:2019897‑M1/69‑1 (MQ=255)
                                                                                                             aaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTgg                          >  2:2201762‑M1/20‑133 (MQ=255)
                                                                                                             aaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGgtg                        >  1:2793847‑M1/20‑135 (MQ=255)
                                                                                                             aaacagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGgt                         <  1:363540‑M1/115‑1 (MQ=255)
                                                                                                             aaaCAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGgtgt                       >  1:2188594/1‑135 (MQ=255)
                                                                                                              AACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTcgatcgg                          <  2:2268947‑M1/132‑4 (MQ=255)
                                                                                                               ACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACg                                                                                                          >  1:1266885‑M1/1‑51 (MQ=255)
                                                                                                                cagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTc                                                                         <  1:1451576‑M1/67‑1 (MQ=255)
                                                                                                                cagctccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCg                     >  2:412186‑M1/17‑135 (MQ=255)
                                                                                                                cAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGt                                                                                                                 <  2:2075751/42‑1 (MQ=255)
                                                                                                                cAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGc                    >  1:2014649/1‑135 (MQ=255)
                                                                                                                CAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCg                     >  2:1359560‑M1/1‑135 (MQ=255)
                                                                                                                     ccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCa                   >  1:1946275‑M1/12‑132 (MQ=255)
                                                                                                                     ccttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCa                   >  2:1517354‑M1/12‑132 (MQ=255)
                                                                                                                     CCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCAt                  >  2:2571911‑M1/1‑133 (MQ=255)
                                                                                                                     CCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCa                   >  1:839712‑M1/1‑132 (MQ=255)
                                                                                                                     CCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCa                   >  2:2293504‑M1/1‑132 (MQ=255)
                                                                                                                      cttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACg                                                                                                          >  2:1621625‑M1/11‑44 (MQ=255)
                                                                                                                      cttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCatcta               <  2:476338‑M1/125‑3 (MQ=255)
                                                                                                                      cttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCatcta               <  1:2310008‑M1/125‑3 (MQ=255)
                                                                                                                      cctgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCa                   >  1:2051635‑M1/11‑131 (MQ=255)
                                                                                                                      CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCa               <  1:2247628‑M1/135‑1 (MQ=255)
                                                                                                                      CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCa               <  1:2274977‑M1/135‑1 (MQ=255)
                                                                                                                      CTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACg                                                                                                          >  1:2524715‑M1/1‑44 (MQ=255)
                                                                                                                       ttgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACg                                                                                                          >  1:1793263‑M1/10‑43 (MQ=255)
                                                                                                                       ctgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTCATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGcatctaa              <  2:2633559‑M1/126‑4 (MQ=255)
                                                                                                                       ctgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCa                   >  2:1723524‑M1/10‑130 (MQ=255)
                                                                                                                        tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt             >  1:1670486‑M1/9‑135 (MQ=255)
                                                                                                                        tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt             >  2:716497‑M1/9‑135 (MQ=255)
                                                                                                                        tgcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAt             >  1:760376‑M1/9‑135 (MQ=255)
                                                                                                                        TGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAAt             >  1:26913‑M1/1‑135 (MQ=255)
                                                                                                                         gcaggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAtt            <  1:1092877‑M1/128‑1 (MQ=255)
                                                                                                                          caggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTc           >  2:658574‑M1/7‑135 (MQ=255)
                                                                                                                           aggatTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTAAATTCTacca                                                               <  2:544169‑M1/77‑1 (MQ=255)
                                                                                                                               CTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCAt         >  1:2752177‑M1/1‑132 (MQ=255)
                                                                                                                                ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc     >  1:301942/1‑135 (MQ=255)
                                                                                                                                ttCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAccc     <  2:305156/135‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGT  >  AssemblyC74_contig_12/58931‑59196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 1044 total aligned reads displayed.
CCCGTTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTT  >  AssemblyC74_contig_12/58913‑59210
                                                                                                                                                     |                                                                                                                                                    
          CTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTC                                                                                                                                           <  SRR22894674.754757/151‑1 (MQ=60)
                  TCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCT                                                                                                                                   <  SRR22894674.1104805/151‑1 (MQ=60)
                       ccgctgatacgctcctggtgctaaaccgtcaattgctagctttccatcttctcctgtagatagcccggtttgtagcgcttctcctactcgttgcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTC                                                                                                                              <  SRR22894674.1221902/57‑1 (MQ=55)
                       aagatcagatggtattcgagatatcccgtcgagtgcccgttgatcggagaggtgtttatgacccccggtttgtctcgtttctccttctcggttcTGTAACTCAAAATCAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTC                                                                                                                              <  SRR22894674.633866/57‑1 (MQ=60)
                            GATACACTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACA                                                                                                                          >  SRR22894674.745255/1‑150 (MQ=21)
                             atacaCTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACG                                                                                                                        >  SRR22894674.394312/6‑151 (MQ=28)
                             atacaCTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACG                                                                                                                        >  SRR22894674.1306901/6‑151 (MQ=28)
                              tacaCTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGA                                                                                                                       >  SRR22894674.1167846/5‑151 (MQ=32)
                                caCTCCTGGTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGATCCATTGGCATCTTGTAACTCAAAAACAGCTCCATGCAGGAATTCCCCGCTTTGGTCATCGATCTTTGTCAACACTACA                                                                                                                     >  SRR22894674.2878061/3‑151 (MQ=22)
                                  ttcctggtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.1397287/67‑1 (MQ=60)
                                  ctcctggtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttctgttcCTCAAAAAAAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.1610107/62‑1 (MQ=60)
                                  ctcctggtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    >  SRR22894674.1833538/84‑150 (MQ=60)
                                  ctcctggtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.1123992/67‑1 (MQ=60)
                                    cctggtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGC                                                                                                                 <  SRR22894674.2789785/70‑1 (MQ=60)
                                    CCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTTCAGAACGGTTCCATTGGCATCTTGTAACCCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGC                                                                                                                 <  SRR22894674.1360429/151‑1 (MQ=60)
                                     ttggtgctaaaccttaatttgcattttttccatcttctcctttattttgaccgttttgtagggtttctccttctgggttcTGTAACACAAAAACTGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCT                                                                                                               <  SRR22894674.147821/71‑1 (MQ=54)
                                        gtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.528674/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.332145/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGC                                                                                                                 >  SRR22894674.2130597/78‑147 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.1738610/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.2113886/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.259491/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.945682/78‑151 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCT                                                                                                               >  SRR22894674.2680582/78‑149 (MQ=60)
                                        gtgctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggctcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGAGCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.1965875/78‑151 (MQ=60)
                                        GTGCTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.2463825/1‑151 (MQ=60)
                                        GTGCTAAGCCGTCAATCGCCAGTTTACCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTAGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCAACGATCTTTGTCAACACGACACCGCCTG                                                                                                              >  SRR22894674.2817380/1‑150 (MQ=44)
                                        GTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGGTCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.1334397/1‑151 (MQ=60)
                                        GTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGG                                                                                                             >  SRR22894674.1820718/1‑151 (MQ=60)
                                        GTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGC                                                                                                                 >  SRR22894674.2336031/1‑147 (MQ=60)
                                        GTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.33884/144‑1 (MQ=60)
                                          gctaaaccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTG                                                                                                           >  SRR22894674.971153/76‑151 (MQ=60)
                                           ctaaaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          <  SRR22894674.204158/76‑1 (MQ=60)
                                           cacacccgtcacatgctcttgttgcctatactgatgaagatagtagtcttgtgtgctattagatttgtctcatagtcacCTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          <  SRR22894674.2568522/72‑1 (MQ=60)
                                           CTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAATAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          <  SRR22894674.2359784/151‑1 (MQ=60)
                                           CTAAGCCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          <  SRR22894674.2960278/151‑1 (MQ=60)
                                           CTAAGCCCTCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          <  SRR22894674.2683862/151‑1 (MQ=60)
                                              acccgtcaattgcaatatctccatattcttctgtattttgagctgtttgtagcgtttctcattctggtttatgtaccaCAAAAACTGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.873549/73‑1 (MQ=60)
                                              aaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.1767199/80‑1 (MQ=60)
                                              aaccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.558861/80‑1 (MQ=60)
                                               cccgtcaattgccagttttccatcgtctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAACCACAGCTCCGTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.1871197/81‑1 (MQ=60)
                                               accgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACC                                                                                                                   >  SRR22894674.2149199/71‑138 (MQ=60)
                                               accgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.533441/81‑1 (MQ=60)
                                               accgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1754787/81‑1 (MQ=60)
                                               accgtcaattgccagttttccaacttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGAAGGTTTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.159527/81‑1 (MQ=60)
                                               GCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      >  SRR22894674.607457/1‑151 (MQ=60)
                                                ctcgtggtctcgtagatgtgtatatgagaccgGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.2401310/119‑1 (MQ=60)
                                                ccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.579480/82‑1 (MQ=60)
                                                ccgtcaattgctagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.410515/82‑1 (MQ=60)
                                                ccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.16711/82‑1 (MQ=60)
                                                ccgtcaattgccagttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1270587/82‑1 (MQ=60)
                                                ccctctctttacaatttgaccacgtcacatgtcttcacactttttttatcgtgttctcgttgttgtgtattatagacatAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1552449/72‑1 (MQ=60)
                                                acgttaatttgtatttttaccaatttttcttttttttttacgggtttgtaggttttcacttataggttttgttacttaaaaaaatCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.2061946/66‑1 (MQ=60)
                                                accttcaattgccttttttccatggtctcgtgtagtttgaccgtttagttgggttactcctccacggtactgtaactaaaaacctgctccTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1100687/61‑1 (MQ=60)
                                                CCGTCAATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1498948/151‑1 (MQ=60)
                                                CCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1285328/151‑1 (MQ=60)
                                                CCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.1638099/150‑1 (MQ=60)
                                                   TCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGAT                                                                                                  >  SRR22894674.2220179/1‑151 (MQ=60)
                                                             gttttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTT                                                                                       >  SRR22894674.683863/57‑151 (MQ=60)
                                                             GTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGT                                                                                        >  SRR22894674.1039273/1‑151 (MQ=60)
                                                             GTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGT                                                                                        >  SRR22894674.1372416/1‑151 (MQ=60)
                                                               tttccatcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCA                                                                                      >  SRR22894674.2248150/55‑151 (MQ=60)
                                                                    atcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATT                                                                                   >  SRR22894674.2209625/50‑149 (MQ=60)
                                                                    atcttctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATT                                                                                   >  SRR22894674.1535316/50‑149 (MQ=60)
                                                                         ctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCAC                                                                            <  SRR22894674.916252/107‑1 (MQ=60)
                                                                         ctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCAC                                                                            <  SRR22894674.1485644/107‑1 (MQ=60)
                                                                         ctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCAC                                                                            <  SRR22894674.1903927/107‑1 (MQ=60)
                                                                         ctcctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCAC                                                                            >  SRR22894674.539565/45‑150 (MQ=60)
                                                                         cacatgtggtaagacctgattgatgtatgtttctattgggatcgtttatgtctataacagataCTTGCAGGACTTCACCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCAC                                                                            <  SRR22894674.2196245/88‑1 (MQ=60)
                                                                         CTCCTGTTGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCAC                                                                            <  SRR22894674.2482622/151‑1 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.2934254/43‑151 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.98968/43‑151 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.840509/43‑151 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1295977/82‑1 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCT                                                                                                               <  SRR22894674.925835/72‑1 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGC                                                                                                                 >  SRR22894674.513030/43‑112 (MQ=60)
                                                                           cctgtagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.2797032/67‑1 (MQ=60)
                                                                           CCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTG                                                                                                           <  SRR22894674.1383440/118‑1 (MQ=60)
                                                                            CTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGC                                                                         <  SRR22894674.2120492/151‑1 (MQ=60)
                                                                               tagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTG                                                                       <  SRR22894674.1915214/112‑1 (MQ=60)
                                                                               tagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.1431590/39‑147 (MQ=60)
                                                                               tagttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTG                                                                                                           <  SRR22894674.2448580/76‑1 (MQ=60)
                                                                                agttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.2840679/38‑146 (MQ=60)
                                                                                agttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTtaatacaaaaaccgctgaca                                                                    >  SRR22894674.2702700/38‑131 (MQ=60)
                                                                                agttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCG                                                                          >  SRR22894674.1387907/38‑145 (MQ=60)
                                                                                GGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.319048/1‑146 (MQ=60)
                                                                                GGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTATCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.1733849/1‑146 (MQ=60)
                                                                                GGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.2321069/104‑1 (MQ=60)
                                                                                GGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.2644588/1‑146 (MQ=60)
                                                                                GGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.1861091/1‑146 (MQ=60)
                                                                                 GTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCG                                                                          >  SRR22894674.2108682/1‑145 (MQ=60)
                                                                                  ttagaccggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCT                                                                    <  SRR22894674.2892499/115‑1 (MQ=60)
                                                                                      acctgatctctctatgtgtctcttgggctctgtgaagtgaataagagacccgTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGTTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTT                                                               <  SRR22894674.1232414/99‑1 (MQ=60)
                                                                                      accggtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTT                                                               <  SRR22894674.2312706/119‑1 (MQ=60)
                                                                                          gtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      >  SRR22894674.1652186/28‑108 (MQ=60)
                                                                                          gtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTACCCCGCTTTGGTCATCGAGCTTTGTCAACACGACACCCCCTGGTGTAAGTCGgtttactttacacaaatcaaacaccccggtcagactacttt                                                           >  SRR22894674.1344240/28‑110 (MQ=60)
                                                                                          gtttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTcggtaacttataaacatatacgttcccacgacacaccaagaatgatgtcgtag                                                          >  SRR22894674.2829341/28‑98 (MQ=60)
                                                                                          gtttgtagagtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTT                                                           <  SRR22894674.2598753/123‑1 (MQ=60)
                                                                                           ACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTT                                                           <  SRR22894674.2520792/150‑1 (MQ=60)
                                                                                            ttgtagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCG                                                         <  SRR22894674.2289933/126‑1 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTC                                                                                              <  SRR22894674.1505520/114‑1 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGAGGTCTCAGCTTTGGTCATCGAacttacaactcacctcaccgagtgttgtaggtcgggtttctttggtgtattcttccacggctgtgggggggggttgg                                                         >  SRR22894674.2306547/1‑74 (MQ=12)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCG                                                         >  SRR22894674.194662/1‑151 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      <  SRR22894674.1068016/106‑1 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGT                                                                                                          >  SRR22894674.427407/1‑102 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCGTGGTGAAAGTCGGTTTTCTTTCGTCAAGTCTACCACCGCTGTCTGACTTCTTTCG                                                         >  SRR22894674.2493377/1‑151 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCG                                                         >  SRR22894674.216002/1‑151 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCG                                                         >  SRR22894674.2236173/1‑151 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.892203/107‑1 (MQ=60)
                                                                                            CTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGACATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCG                                                         >  SRR22894674.2884590/1‑151 (MQ=60)
                                                                                               tagcgtttctccttctcggttcTGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGATTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTTAGTCGGTTTTCGTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATC                                                      <  SRR22894674.1844565/128‑1 (MQ=60)
                                                                                                 gcgtttctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTAGATCT                                                     >  SRR22894674.144578/21‑150 (MQ=60)
                                                                                                      tctccttctcggttcttgaacaCAAAAACATCTTCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACT                                               <  SRR22894674.498107/129‑1 (MQ=60)
                                                                                                       ctccttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACT                                               >  SRR22894674.2713558/15‑150 (MQ=60)
                                                                                                          cttctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGAT                                           >  SRR22894674.2471887/12‑151 (MQ=60)
                                                                                                          CATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGAT                                           >  SRR22894674.300450/1‑151 (MQ=60)
                                                                                                            tctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTG                                         >  SRR22894674.419110/10‑151 (MQ=60)
                                                                                                            tctcggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTC                                              <  SRR22894674.594175/137‑1 (MQ=60)
                                                                                                             TGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGG                                        <  SRR22894674.2774994/151‑1 (MQ=60)
                                                                                                               cggttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTG                                      >  SRR22894674.2275368/7‑151 (MQ=60)
                                                                                                               GCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTG                                      >  SRR22894674.2052812/1‑151 (MQ=60)
                                                                                                                 gttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGT                                     >  SRR22894674.439662/5‑150 (MQ=60)
                                                                                                                 gttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGG                                        >  SRR22894674.2324579/5‑147 (MQ=60)
                                                                                                                 gttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     >  SRR22894674.2437655/5‑86 (MQ=60)
                                                                                                                 gttcTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATC                                                                                                                                    >  SRR22894674.2509845/5‑55 (MQ=39)
                                                                                                                    TTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTctgttagacctctcacagccaccgttggtgaagga                                 >  SRR22894674.1793189/1‑116 (MQ=60)
                                                                                                                    CTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCA                                 >  SRR22894674.399251/1‑151 (MQ=60)
                                                                                                                    CTGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTC                                                                                                     <  SRR22894674.1468353/83‑1 (MQ=60)
                                                                                                                     gtgacCTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGTTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCA                                 <  SRR22894674.808919/145‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGTATTTCCCCGCTTTGGACATCGATCTTTGACAACACTACACC                                                                                                                   >  SRR22894674.705518/1‑68 (MQ=40)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCAT                                <  SRR22894674.1564106/151‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACA                                                                                                                          >  SRR22894674.2924392/1‑61 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACA                                                                                                                          >  SRR22894674.2168767/1‑61 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACA                                                                                                                          >  SRR22894674.2662063/1‑61 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAA                                                                                                                            >  SRR22894674.1574439/1‑59 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAA                                                                                                                            >  SRR22894674.1000014/1‑59 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTC                                                                                                                              <  SRR22894674.2022183/57‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGA                                                                                                                                      <  SRR22894674.378680/49‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCAT                                <  SRR22894674.1323764/151‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACAC                                                                                                                    <  SRR22894674.364218/66‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACAC                                                                                                                         <  SRR22894674.2349749/61‑1 (MQ=60)
                                                                                                                     TGTAACTCAAAAACAGCTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTC                                                                                                                              <  SRR22894674.1994588/56‑1 (MQ=51)
                                                                                                                     TGTAACTCAAAAACAGCACCTTGCAGGATTTCCCCGCTTTGGTCATCGATCT                                                                                                                                   >  SRR22894674.2743564/1‑52 (MQ=49)
                                                                                                                          CTCAACACCAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTATGTCCCCTCGACAGGGCATGGGGTTATTAGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCTAACTCGATTGGTGTCGCATCTAAT                           <  SRR22894674.354762/151‑1 (MQ=59)
                                                                                                                          CTCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAAT                           >  SRR22894674.1894383/1‑151 (MQ=60)
                                                                                                                          CTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGG                                        <  SRR22894674.570031/138‑1 (MQ=60)
                                                                                                                          CTCAAAAACAGCTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGAAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTACGATCTCAAACACGATCGGTGTAGCATCAAAT                           >  SRR22894674.2944208/1‑151 (MQ=60)
                                                                                                                           TCAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATT                          <  SRR22894674.1936223/151‑1 (MQ=60)
                                                                                                                            CAAAAACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTC                         <  SRR22894674.2076292/151‑1 (MQ=60)
                                                                                                                            CAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTC                         <  SRR22894674.647702/151‑1 (MQ=60)
                                                                                                                            CAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTC                         <  SRR22894674.2541264/151‑1 (MQ=60)
                                                                                                                               AAACAGCTCCTTGCAGGATTTCCCCGCTATGGTCATCTATCGTTGTCAACACGACACCTCCTGGTGTAAGTCGGTGTTCTATCGACAATTCTACAACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCAACAAAATCATA                      >  SRR22894674.2005718/1‑151 (MQ=60)
                                                                                                                                AACAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATAC                     <  SRR22894674.1252636/151‑1 (MQ=60)
                                                                                                                                AACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATAC                     <  SRR22894674.2441370/151‑1 (MQ=60)
                                                                                                                                  AAGCTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.618804/151‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCN                   <  SRR22894674.2872711/148‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.1979625/148‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.1588095/148‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACC                    <  SRR22894674.2379579/147‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACC                    <  SRR22894674.778259/147‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.2906065/148‑1 (MQ=60)
                                                                                                                                     CTCCTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACC                    <  SRR22894674.2809414/147‑1 (MQ=60)
                                                                                                                                     CTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.2087794/147‑1 (MQ=60)
                                                                                                                                     CTCC‑TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   <  SRR22894674.168852/147‑1 (MQ=60)
                                                                                                                                       CCTTGCAGGTCTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCtattg              <  SRR22894674.2610596/151‑6 (MQ=60)
                                                                                                                                        CTTGCAGGATTTCCCCGCTTTGGTCATCGTTCTTTGTCAACTCGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGATGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.2727108/151‑1 (MQ=60)
                                                                                                                                        CTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.1874678/151‑1 (MQ=60)
                                                                                                                                        CTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.773036/151‑1 (MQ=60)
                                                                                                                                        CTTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.2027552/151‑1 (MQ=60)
                                                                                                                                        CTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGG             <  SRR22894674.1090193/151‑1 (MQ=60)
                                                                                                                                        CTTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.1778552/151‑1 (MQ=60)
                                                                                                                                        CCTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.2548185/151‑1 (MQ=60)
                                                                                                                                        CCTGCAGGATTTCCCCGCTTTGGTCATCGAACTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAATCTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             <  SRR22894674.1458516/151‑1 (MQ=60)
                                                                                                                                         TTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.657737/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.737990/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.2186367/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.1215438/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTC                         >  SRR22894674.2854403/1‑138 (MQ=60)
                                                                                                                                         TTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGG             >  SRR22894674.1028560/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGG             >  SRR22894674.1011262/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGG             >  SRR22894674.1710687/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGG             >  SRR22894674.2559366/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTCATTCATACCCTATTGG             >  SRR22894674.861111/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.794754/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.1424469/1‑150 (MQ=60)
                                                                                                                                         TTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATCGGTGTCGCAT                                <  SRR22894674.731540/131‑1 (MQ=60)
                                                                                                                                         TTGCAGGACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGT                                                                                                      >  SRR22894674.1701299/1‑61 (MQ=60)
                                                                                                                                          TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCTATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCG           <  SRR22894674.1195840/151‑1 (MQ=60)
                                                                                                                                          TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCG           >  SRR22894674.719857/1‑151 (MQ=60)
                                                                                                                                          TGCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCG           <  SRR22894674.1051324/151‑1 (MQ=60)
                                                                                                                                          TGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCG           <  SRR22894674.263173/151‑1 (MQ=60)
                                                                                                                                           GCAGGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCG           >  SRR22894674.1789443/1‑150 (MQ=60)
                                                                                                                                              GGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTG       >  SRR22894674.458928/1‑151 (MQ=60)
                                                                                                                                              GGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTG       >  SRR22894674.1151856/1‑151 (MQ=60)
                                                                                                                                              GGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTG       >  SRR22894674.1540758/1‑151 (MQ=60)
                                                                                                                                              GGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCGTG       >  SRR22894674.472563/1‑151 (MQ=60)
                                                                                                                                              GGATTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.56195/1‑145 (MQ=60)
                                                                                                                                               GACTTCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGAC‑TCCTTCGATCTCAAACTCGATCGGTGTCGCATCTAATTCATACCCTATTGG             >  SRR22894674.1180947/1‑143 (MQ=60)
                                                                                                                                                   TCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTTAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCCTATTGGCGCTTGAGTTT  >  SRR22894674.2863341/1‑151 (MQ=60)
                                                                                                                                                   TCCCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGGTTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCTAATTCATACCC                   >  SRR22894674.2616726/1‑134 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CCCGTTGGCGCTTGAGTTTCTACCAGCTGATATGCTCCTGGTGCTAAGCCATCTATCGCCAGTTTTCCATCTGCTCCTGTGGTTAGACCTGACTGCAGAACGGTTCCATTGGCATCTTGTAACTCAAAAACAGCTCCTTGCAGGACTTCTCCGCTTTGGTCATCGATCTTTGTCAACACGACACCGCCTGGTGTAAGTCGATTTTCTTTCGTCAATTCTACCACCGCTGTCTGACTTCTTTCGATCTCAAACTCGATTGGTGTCGCATCCAATTCATACCCCGTTGGCGCTTGGGTTT  >  AssemblyC74_contig_12/58913‑59210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑