Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F3 I0 R1	57	144.6	2548318	98.2%	2502448	268.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,304,520	G→T	4.2%	intergenic (+129/+586)	eco → / ← mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,304,520	0	G	T	4.2%	294.9 / 3.4	95	intergenic (+129/+586)	eco/mqo	ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand): ref base G (49/42); new base T (2/2); total (51/44)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01

AAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTT  >  NC_000913/2304237‑2304807

aagaGAAGAAATTTGTCACCGCGTATCTGGTCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCa                                                                                                                                                                                                                                                                                               <  1:309092/287‑1 (MQ=255)

    gaagaaATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                                                                                                                                          <  1:952324/288‑1 (MQ=255)

    gaagaaATTTGTCACCGCGTATCGGGGCGATGCTGGAATGCTGCGTGACAACAGCAAGCTGCCGAGCGGGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACGGCCGTGAAGTGCGGCACCCCGTAGGGCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCAcccc                                                                                                                                                                                                                                                                                           <  2:89700/287‑1 (MQ=255)

     aagaaATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTa                                                                                                                                                                                                                                                                                        >  1:686541/1‑289 (MQ=255)

         aaTTTTTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATGGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTTCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAgg                                                                                                                                                                                                                                                                                      <  1:138675/287‑1 (MQ=255)

                 aCCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAg                                                                                                                                                                                                                                                                             >  1:264118/1‑288 (MQ=255)

                   cgcgTATCTGGGCGATGCTGGAATGCTGCGTTACAACATCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAATGCGGTCACGCCGCATCCGACATCCTACGCCCGAGCCGGT‑TGCCTGATGCGACGCTTGCGCGTCTTATCATGCCTACACCGCTGTCAAGTGCGTCACCCCGTACGTCAGACAAGTCg                                                                                                                                                                                                                                                                          >  1:657249/1‑289 (MQ=255)

                   cgcgTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCg                                                                                                                                                                                                                                                                          >  1:670474/1‑289 (MQ=255)

                         tCTGGGCGATGCTGGAATGCTGCTTTACAACAGCAAGCTGCCGATCGTGGGGTATACGCCAGACAATGGAGGTGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAAGTGAC‑AACGCGGTAGTGCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcg                                                                                                                                                                                                                                                                    <  1:633889/289‑1 (MQ=255)

                          cTGGGCAGTGATGGAATGCTGCGTTACACCAGCAAGCTGCCGATCGTGGTGTATACGCCAGACATTGTAGATGTGAAGTACCGGGTCTGGAAGGG‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACGGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCAGCCGACATCCAACGCCCGGGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgc                                                                                                                                                                                                                                                                   <  1:393057/289‑1 (MQ=25)

                            ggggGATGCTAGAATGCTGCATTACAACAGCAAGCTGCCGAGCGTGGTGTAAACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAATTGCGGCACCCCGTAGGGCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAcgccg                                                                                                                                                                                                                                                                 <  1:687541/289‑1 (MQ=255)

                             ggCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGt                                                                                                                                                                                                                                                                                     <  2:259127/268‑1 (MQ=255)

                             ggCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGt                                                                                                                                                                                                                                                                                     >  1:259127/1‑268 (MQ=255)

                                  tGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                                                                                                                              >  1:632817/1‑270 (MQ=255)

                                  tGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                                                                                                                                              <  2:632817/270‑1 (MQ=255)

                                            ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAAc                                                                                                                                                                                                                                                  >  1:818262/1‑288 (MQ=255)

                                            ctgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCCTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACACCCAAc                                                                                                                                                                                                                                                  >  1:459376/1‑288 (MQ=255)

                                             tgcGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACg                                                                                                                                                                                                                                                 >  2:1171478/1‑288 (MQ=255)

                                                    caacaGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGcc                                                                                                                                                                                                                                         >  1:728521/1‑289 (MQ=255)

                                                       caGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGt                                                                                                                                                                                                                                      >  1:440290/1‑289 (MQ=255)

                                                         gCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCCACCCGGtt                                                                                                                                                                                                                                     >  1:738678/1‑288 (MQ=255)

                                                            aGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGCGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTTAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCCCATCCGACACCCAACGCCCGACCCGGTTGccc                                                                                                                                                                                                                                 >  2:939292/1‑288 (MQ=255)

                                                               tGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGAt                                                                                                                                                                                                                              >  1:1116816/1‑289 (MQ=255)

                                                                  cGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCg                                                                                                                                                                                                                           >  1:340928/1‑289 (MQ=255)

                                                                         ggtgTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  1:934462/289‑1 (MQ=255)

                                                                         ggtgGATACGCCAGAAAATGTAGATGTTAAGTACCGCGGCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACTCGGTAGTTCTCTAAATGGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCAAGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  1:943724/289‑1 (MQ=255)

                                                                          gggtagccgcgagacataagagagctgAAGTAGCGCGTGTGGCAGGC‑AGAAGAGAAAATGGAC‑ACCGGGGTAGTTCGCGAAACGTCCGTGAAGTGGGGCACACCGTAGGTCAGATAAGGCGCGAAGGCCGTATCCGACATCGAACGCCCGAGCCGGT‑TGCCTAATGCGCCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGTCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTgg                                                                                                                                                                                                                   <  2:575017/263‑1 (MQ=17)

                                                                               taCGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGcgcg                                                                                                                                                                                                               >  1:1225336/1‑287 (MQ=255)

                                                                                   ccAGACAATGTGGATGCGCAGTAACTGGTCAGGAAGGG‑GGAAG‑GGAAACTGACAAACGCGTGGGTTGGCTAAACTACAGTGAAGTGCGGCACCCCGGAGGTCAGCAAAGGCGGCCACGCCGCAGCCGACACCCACCGCCCGGGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:599398/289‑1 (MQ=14)

                                                                                    cAGACAATGTGGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGGGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATc                                                                                                                                                                                                        <  1:806749/289‑1 (MQ=255)

                                                                                    cAGACAATGGAAAGATGGCGTACCGGGCCTGGAGGGC‑GGAAGAGACACTTGAC‑GTCGTGGGGGTTCGCTGAACTGCCGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCAGCGTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTGATCAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:340928/288‑1 (MQ=17)

                                                                                    aagaaagtgtgGATGTAAAGTCGCGAGTGTGGAGGGG‑GGAAGAGCAAATTGGC‑ACCTGGGGAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGT‑GGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:962401/281‑1 (MQ=17)

                                                                                     aggggacagcgtgtggatgtgccGCGTCT‑GAAGGCGGGAAGTGGAATTTGTC‑ACCTCGGAGGTGCGCTAAACGGCCGTGACGCTCGCAACCCCGAGGGTCAGACAAGGCGGTCAAGCCGCATCGGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  2:1196685/266‑1 (MQ=14)

                                                                                      gACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCAGACATCCAACGCCCGAGCCGGTTGCCTGATGCA‑ACGCTGGCGCGTCTTAtagg                                                                                                                                                                                                      >  1:577134/1‑286 (MQ=255)

                                                                                       aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCTACGCTGGCGCGTCCTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACCCCGCCTCCGACATCCAACGCCCGCGCCGGTTGCCTGATGGC‑ACACTGGCCCGTCCTAACAg                                                                                                                                                                                                      >  2:913359/1‑288 (MQ=21)

                                                                                       aCAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAg                                                                                                                                                                                                      >  1:123064/1‑288 (MQ=255)

                                                                                              gaatgtgagggagcagtgcTGGAAGGC‑GGAAGGTAAAATGGAG‑ACGGGGGGAGTGAGCTAAATTGCCGTGAGGTGGGGCACCCCGTAGGTGAGACAAGGGGGGCACGCCGCAGCCGACAGAAAACCCCCGGGCGGGT‑GGCCTGAGGCGAAGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               <  2:335278/270‑1 (MQ=14)

                                                                                              aGATGTGAGTTACGCGGTCTGGAAGGC‑TGAATGTAAAATGGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGTTCACGCCGCATCCGACATCCATCGCCCGGGCCGTT‑TGCCTGGTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               <  1:214521/288‑1 (MQ=17)

                                                                                                 tgtgAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACACGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGCTGCG‑CCGCTGGCGCCTCTTATCCGGCCTACAcc                                                                                                                                                                                             >  1:508939/1‑287 (MQ=255)

                                                                                                  gtgAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTCCAcc                                                                                                                                                                                             >  1:76749/1‑286 (MQ=255)

                                                                                                  gtgAAGTACCGCGGCTGGAAGGA‑GGAAGAGAAAATTGAC‑AACGCGGAAGTTCGCTAAACTGCCGTGAAGGGCTGCACCCCGTAGGTCAAACAAGGCGGTCACGCCGCATCCGACAGCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGATGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGGCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCt                                                                                                                                                                                          <  1:320320/289‑1 (MQ=255)

                                                                                                     aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGTGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCATCCAACGCCCGCGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTCTCAGGCCTACACCGCtgtg                                                                                                                                                                                       >  1:335278/1‑289 (MQ=255)

                                                                                                     aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCtgtg                                                                                                                                                                                       >  1:352060/1‑289 (MQ=255)

                                                                                                     aaGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCAGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCtgt                                                                                                                                                                                        >  1:706386/1‑288 (MQ=255)

                                                                                                       gTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGaa                                                                                                                                                                                     >  2:858165/1‑289 (MQ=255)

                                                                                                         aCCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACACGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               >  1:1196685/1‑277 (MQ=255)

                                                                                                          ccGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGACACCCCGTAGGTCAGACAAGGCGGTCACGCCGCTACCGACATCCCACACCCGACCCGCTTGCCTGATGCGCACG‑TCGCGCGTCTCATCAGTCCCACCCCGTCGTGAAgc                                                                                                                                                                                   >  2:819926/1‑287 (MQ=18)

                                                                                                             cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:925627/1‑288 (MQ=255)

                                                                                                             cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:1252365/1‑288 (MQ=255)

                                                                                                             cgTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCt                                                                                                                                                                                >  1:446792/1‑288 (MQ=255)

                                                                                                                 gtgagaggggaaaGGGAAACTGGGC‑ACCGCGGGGGTTCGCTAAAGCGCCGTGAAGAGCGGCACCCGGTAGGTCAGACAAGGCGGTCACGCCGCGTCCGACATCCAACGCCCGAGCCGGT‑GGCCTGATGCGACGCTGGCGCGTCGTATCAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                                                                                                                                             <  2:264118/277‑1 (MQ=14)

                                                                                                                        c‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAgg                                                                                                                                                                    >  1:705848/1‑289 (MQ=255)

                                                                                                                                  aaaTTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATaa                                                                                                                                                            <  1:858165/288‑1 (MQ=255)

                                                                                                                                      tGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                        <  1:904907/272‑1 (MQ=255)

                                                                                                                                      tGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCg                                                                                                                                                                        >  2:904907/1‑272 (MQ=255)

                                                                                                                                                     tgtgctaactcggcgggAAGTGCGGCACCCCGAAGGTAAGAAAAGGCGGTCAGACCGCGTCCGAGATCCAACGCCTGAGCCGGT‑GGCCTGAGGCGACGCTGGCGCGTCTTAACAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  2:123064/272‑1 (MQ=14)

                                                                                                                                                          tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                              <  1:331109/263‑1 (MQ=255)

                                                                                                                                                          tAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGAt                                                                                                                                                              >  2:331109/1‑263 (MQ=255)

                                                                                                                                                           aaccttccGTTAATTGCGGCACCCCCTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGTGCCGGT‑TGCCTGATGCGACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                   <  1:1142326/283‑1 (MQ=255)

                                                                                                                                                            aaCTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGAGAAGGCGGTGACGCCGGATCCGACATCTa                                                                                                                                  >  1:247206/1‑289 (MQ=255)

                                                                                                                                                                       aGTGCGGCACCCCGTAGGTCAGAAAAGGCGGTCACGCCGCCTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGc                                                                                                                        <  1:913359/288‑1 (MQ=255)

                                                                                                                                                                          caggCACCCGTCAGGTTGGACAAGGCGGCAAGGCCACATCCGTCATACAACCCCCGAGCCGGT‑GGCCGGATGGGACGCTGGCGCGTCTTAACAGGCCTAAACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGAGAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                     <  2:738678/286‑1 (MQ=14)

                                                                                                                                                                           cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                   <  1:1171478/289‑1 (MQ=255)

                                                                                                                                                                           cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                   >  1:563177/1‑289 (MQ=255)

                                                                                                                                                                              ccccccGTAGGTCAGGTAAGGCGGTCACGCCGCGTGCGGCATCGAAGGGCCGAGAGGGA‑TGCCGGGGGGGAAGCTGGCAGGTGGGAGCAGGGCTACACCGCTGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                 <  2:670474/286‑1 (MQ=14)

                                                                                                                                                                              cACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                  >  1:460140/1‑287 (MQ=255)

                                                                                                                                                                                ccccGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTAt                                                                                                                                                                                                         <  1:397357/198‑1 (MQ=21)

                                                                                                                                                                                   cGTAGGTCAGACAAAGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCCGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACCCCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCCTCCGCCACACAACGCCCCAGCCGGTTGCCTGTTg                                                                                                           >  1:355619/1‑289 (MQ=14)

                                                                                                                                                                                        gTCAGCCAAGGCGGTGACGCCGCCTCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  1:359186/253‑1 (MQ=255)

                                                                                                                                                                                          cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTaca                                                                                                                                                                                               >  2:397357/1‑198 (MQ=21)

                                                                                                                                                                                           aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTg                                                                                                   >  1:10743/1‑289 (MQ=255)

                                                                                                                                                                                             aCAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAAGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATACGGCGGTTACGCCGCATCCGACATCGAACTCCCTAGCCGGTTGCCTGATGCGACGCTGgc                                                                                                 >  1:132602/1‑289 (MQ=255)

                                                                                                                                                                                                      cccggccgcACCCGGCACCCAAC‑CCCGTGGCGGTGCGCGTGAGGGGACTCGGGCACGTCTGATCTGGCCTAGCCGGCGGTGAAGTGCGCGACCCCGTAGGGCCGACAAGGCGGTCACGCCGCATCCGACACCCAACGCCCGGGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTGATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAt                                                                                        <  2:577134/285‑1 (MQ=11)

                                                                                                                                                                                                                  cgccATCCACCGCCCGAGCCGGT‑TGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTaca                                                                              <  1:308041/284‑1 (MQ=255)

                                                                                                                                                                                                                                   ggcggggtGCCTGGTGGGACGCTGCGGCGTCTTCTGAGCCCTACACCGCCGTGAAGGGCGGCACCCCGTGGGTCAGACAAGGGGGTCACGCCGCATCCGACATCCAACGCCCGGGCCGGTGGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTcc                                                             <  2:925627/281‑1 (MQ=11)

                                                                                                                                                                                                                                   gCCGGT‑AGCGTGATGAGACGCTGGGGCGTGTGATCATGCCTAAACCGCTGGGAAGTGCGGCACCCCGGAGGTCATAAAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTGCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCa                                                            <  2:705848/288‑1 (MQ=11)

                                                                                                                                                                                                                                        t‑tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          >  2:134500/1‑205 (MQ=255)

                                                                                                                                                                                                                                        t‑tGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATcc                                                                                                                                          <  1:134500/205‑1 (MQ=255)

                                                                                                                                                                                                                                            ccgatggggacagggggacgggttAGCAGGCCTAAACCGCTGGGAAGGGCGGCGCCCCGGAGGTCAGACGAGGCGGGCACGACGCAGCCGAGAGCCAACGCCCGAGCCGGTTGCCGGATGCG‑AAGCTGGCGCGGCTTTTCAGGCCTACCCCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTGGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAg                                                    <  2:1252365/266‑1 (MQ=11)

                                                                                                                                                                                                                                                    gACGCGGGCGCGTCTTATCAGGACTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATaa                                           <  1:819926/289‑1 (MQ=38)

                                                                                                                                                                                                                                                           gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTCGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCCGCTGCGACGCTGGCGCGTACTATCACGCCTCCCCCGCTGTGAACTCCCACACCCCAGAGGTTggctcagcgcgg                                     >  2:842405/1‑278 (MQ=11)

                                                                                                                                                                                                                                                              gtacTTTTCGGGCCTAGACCGCGGTGAAGGCGGGCACCCCGTAGGTCGGGCAAGGCGGGCACGCCGCAGCCGACGTCCAACGCCCGGGCCGGTGGCAGGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTAcg                                 <  2:728521/286‑1 (MQ=11)

                                                                                                                                                                                                                                                                     tCAGGCCCACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGATAAGGCGGTTACGCCGCATCCGACACCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCAACCCGTAGGTTAGATGAGGCGGTTACGCCGCAt                           >  1:32839/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                              cacTGCTGGGAAGTGCGGCACCCCGTAGGTCGGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCGGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCa                 <  1:784898/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                              cacCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACCTCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCa                 >  1:110869/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                                 cGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACg              <  1:781851/289‑1 (MQ=40)

                                                                                                                                                                                                                                                                                   cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGTCTCATGCGACGCTGGCCCGTCTTATCAGGCCTACACCGCTGAGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGGTACCCCGGATTCGACATCGAACGt             >  1:72807/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                                   cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt             >  1:279219/1‑288 (MQ=40)

                                                                                                                                                                                                                                                                                   cTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACgc             >  1:853962/1‑287 (MQ=25)

                                                                                                                                                                                                                                                                                       gacgggCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGCCATCCACCGCCCGCGCCGGTTGCCTGATGCG‑ACGCTGGCCGGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa         <  2:1043044/283‑1 (MQ=255)

                                                                                                                                                                                                                                                                                        aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAg        >  1:959788/1‑288 (MQ=255)

                                                                                                                                                                                                                                                                                        aaGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAg        >  1:350481/1‑288 (MQ=21)

                                                                                                                                                                                                                                                                                           tGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGCCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCAGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGCAGTTCTCCACCCCGCAGGTTGGATAAGTCGGTTACGCCGCATCCGACCTCCAACGTCCGAGCCgg    >  1:283291/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                                             cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                >  1:1251310/1‑211 (MQ=39)

                                                                                                                                                                                                                                                                                             cGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa                                                                                <  2:1251310/211‑1 (MQ=39)

                                                                                                                                                                                                                                                                                              ggCACCCGGGAGTGCGGACAGGGCGGTAAGGCCGCAGCGGAGGGCAAGCGCCCTAGCCGGTTGCCGGAGGCG‑AAGCTGGCGGGGCATAACAGGCCTAAACCGCTGTGAAGTGCCGCACCCCGGAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTATCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGGCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt  <  2:508939/288‑1 (MQ=11)

AAGAGAAGAAATTTGTCACCGCGTATCTGGGCGATGCTGGAATGCTGCGTTACAACAGCAAGCTGCCGATCGTGGTGTATACGCCAGACAATGTAGATGTGAAGTACCGCGTCTGGAAGGC‑GGAAGAGAAAATTGAC‑AACGCGGTAGTTCGCTAAACTGCCGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGT‑TGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGGCACCCCGTAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCG‑ACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTT  >  NC_000913/2304237‑2304807

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A