Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,597 T→G 13.2% V1021G (GTG→GGG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,5970TG13.2% 269.0 / 5.4 118V1021G (GTG→GGG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (43/57);  new base G (14/4);  total (57/61)
Fisher's exact test for biased strand distribution p-value = 9.37e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAA  >  NC_000913/489378‑489831
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
  ggCGATACGGTAACCATTGGTAGCTTCGCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                          <  1:584079/239‑1 (MQ=255)
   ggttttcGGTAACCTTTGGTGCCTTCCCGGGGAACGTAAGGTAGATCCTGTTTCGGGCGACAACGATTACCGATTTTGATCGCAAAGAAGTGATGATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAg                                                                                                                                                                                                                         <  2:754428/233‑1 (MQ=255)
   gggATGCGGTAACCATTTGGAGCTTCTCGGAGGCGGTAAGTTAGATCCGTAGTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCATCTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGGCTGAg                                                                                                                                                                                                                         <  2:361841/237‑1 (MQ=255)
   gCGATACGGGAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTAATCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCAACCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                          <  1:61737/238‑1 (MQ=255)
     tgtatggTACCCTTTGGTGTCTTCTCGGGGGCGGTAAGGAAGATCCGTATTCGGTCGACAACGCTTACCGATTTCGATCGCCAAGAAGTGAACATCCCGAACAAAGCC‑TTTGTTGCCGAGCGTCTGATCATCTGGTCGTTGACTGACATTACTACGCGTCTGGTGATCCGTTTCGGCGTGGCCTATGGCCCCGATCTGGAAAAAGTGCGTAAAGTGTTACTTAAGGCGGCGACTGAGCa                                                                                                                                                                                                                       <  2:901228/234‑1 (MQ=255)
        aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTAAAGGCGGCGACTggggcccc                                                                                                                                                                                                                    >  1:1021285/1‑232 (MQ=255)
          ggTAACCATTGGTAGCTGCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa                                                                                                                                                                                                                 <  1:721457/239‑1 (MQ=255)
             aaCCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAggg                                                                                                                                                                                                              >  1:765442/1‑239 (MQ=255)
              aCCATTGGTAGCTTCTCGGTGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑ATTGTTACCTAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACCCGTCTGGCGCTCAGTCTCGGCGTGGCCATCGGGCCCGTCATGGGACAAGAGCGGAAACGTCTACTGAAGGCGGGACCTGAGCAccccacggg                                                                                                                                                                                                              >  2:219839/1‑233 (MQ=255)
               ccATTGGGAGCTTCGCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTg                                                                                                                                                                                                            <  1:212182/239‑1 (MQ=255)
                   tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCTTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGCCTGAGAACCC‑AAGGGAGGTGc                                                                                                                                                                                                        >  1:178074/1‑239 (MQ=255)
                   tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCCCCGATCTGGGAAAAGGGGGTAAAGGGTTACTGAAGGCGGGGACGGGGGACCC‑AAggggagggc                                                                                                                                                                                                        >  2:442352/1‑233 (MQ=255)
                    ggTAGCTTTCCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCa                                                                                                                                                                                                       <  1:977838/239‑1 (MQ=255)
                    ggTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCCCGGCGGGGCCTATGGCCCCGCTCTGGTAAAAGGGGGTAAATGGTTACCGAAGGGGGGCCATGAACACCCAAAGGGTgtgac                                                                                                                                                                                                        >  2:507669/1‑235 (MQ=255)
                          ttCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTCTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAAcc                                                                                                                                                                                                 >  1:874145/1‑239 (MQ=255)
                          ttCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAAcc                                                                                                                                                                                                 >  1:314996/1‑239 (MQ=255)
                            tccGGGGACGGTAAATAAGATCCGTATTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCaa                                                                                                                                                                                               <  2:314996/237‑1 (MQ=255)
                                               aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGGGCCTGAGCACCC‑AAGGAGGATGCCAGAAACAAAGGCGGAAGGCTTCTTTAc                                                                                                                                                                            >  2:504411/1‑239 (MQ=255)
                                               aTCCGTATTCGTGCGACAACGATCACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATTTGGAAAAAGGGCGCAAAGTGTTAATGAAGGGGGCGACTGAGCACCC‑AAGGAGGAGTCACGAAACAAGGCCGGAAgcaaacatgaa                                                                                                                                                                            >  2:891520/1‑229 (MQ=255)
                                                tCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGACCCAATGCCGGAACTCTTCTTTACg                                                                                                                                                                           >  1:915508/1‑239 (MQ=255)
                                                 ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGACAAGATGCGTAAAGGGTTACTGAAGGCGGCGACTGAGAACCC‑AAGGGGGAGGAAGGAACCACTGCCGGAAGTCTTCTTTACgg                                                                                                                                                                          >  1:574181/1‑239 (MQ=255)
                                                 ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGGGCCTGAGCACCC‑AAGGGGGATGCAAGAACCAATGACGGaaagattttttaggg                                                                                                                                                                          >  2:389561/1‑226 (MQ=255)
                                                 ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGCTTTCTTTACg                                                                                                                                                                           >  1:421261/1‑238 (MQ=255)
                                                 ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTTAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCAAGAACCAAGACAGGAAGACTTCTTTAAgg                                                                                                                                                                          >  2:342279/1‑239 (MQ=255)
                                                    tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAt                                                                                                                                                                       >  1:52222/1‑239 (MQ=255)
                                                    tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGACGTTGACTGACACTACTACGCGTCTGGAGATCCGTCTCGGCTTGGCCTAGCGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTTGGCACCC‑AAGGGTGATCCAAGGACCAAGTCCGGGATGCTTCTTTACGGCAt                                                                                                                                                                       >  1:487894/1‑239 (MQ=255)
                                                      tttgggCGACAACGGTTTCCGATTTCGTACGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAttt                                                                                                                                                                     <  2:1068396/234‑1 (MQ=255)
                                                         gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGCAACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGCTCTGGAAAAAGTGCGTAAAGGGTTACTGACGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGCACCAAAGCCGGAGACCTTCTTTCAGGCCTTTGGt                                                                                                                                                                  >  2:652544/1‑239 (MQ=255)
                                                         gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCCCGAACCAATGCCGGAAGTCTTCTTTACGGCATTTggg                                                                                                                                                                  >  1:1068396/1‑238 (MQ=255)
                                                         gTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGGCTCGGCGTGGCCTATGGCTCCGTCCTGGGAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGGGCACCC‑AAGGGGGAGGCCCAAACCAAAGCCGGAAGTCTTCTTTACGGCATTTgg                                                                                                                                                                   >  2:424761/1‑238 (MQ=255)
                                                          tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGGAAAGTGTTACTGAAGGCGGCGACTGGGCACCC‑CAGGGTGATGCACGAACCAATGCCGGAAGTCTTTTTTACGGCATTTggg                                                                                                                                                                  >  2:753128/1‑237 (MQ=255)
                                                            cGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑CAGGGGGATGCCGGAACCAAGGCGGAAAGCCTTCTTTCCGGCATTTGGTGcc                                                                                                                                                               >  1:65269/1‑239 (MQ=255)
                                                              aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑CAGGGGGAGGCACGAACAAATGCCGGAAGGCTTTTTTACGGCATTTgtggcaac                                                                                                                                                             >  2:456265/1‑232 (MQ=255)
                                                               aaaCGATTACCGATTTCGATCGGAAAGAAGTGATCATCCCGAACAAAGCG‑GTGGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGAAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                                                                                                                                             <  1:629581/237‑1 (MQ=255)
                                                                    ttttccGATTTCGATCGCAAAGAAGTGATCCTCCCGAACAAAGGC‑TTTGTGACCGGGCGTCTGATCCACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                       <  2:968809/235‑1 (MQ=255)
                                                                     ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                       <  1:208660/238‑1 (MQ=255)
                                                                     ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                       <  1:1014053/238‑1 (MQ=255)
                                                                       aCCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGTGCCAGCCCGTTggg                                                                                                                                                    >  1:832164/1‑238 (MQ=255)
                                                                                gATCCCAAAGAAGTGTTCCTCCCGAAAAAAGCT‑TTTTTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                           <  2:421261/239‑1 (MQ=255)
                                                                                 aTCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCTTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGCCGACTGAGCACCC‑AAGGGTGATGCAAGAACCAATACCGGAAGGATTCTTTCCGGCATTTGGGGCCGGCagttttgacactaagcag                                                                                                                                          >  2:706520/1‑222 (MQ=255)
                                                                                  tcccAAAGAAGTTGTCATCCCGAACAAAGCT‑TTTGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc                                                                                                                                         <  2:178074/236‑1 (MQ=255)
                                                                                           agtgtctctcCCG‑ACAAAGGGTTTTGTTGCCTGGGGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGAGCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                                  <  2:487894/229‑1 (MQ=255)
                                                                                           agTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCGGATCAACTGGTCGTTGACTGACGCTACTACGCGTCTGGTGATCCGTCGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACGC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                                  <  1:1054756/237‑1 (MQ=255)
                                                                                                    cccGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCCCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGTGAATGCCCGAACCAATGCCGGAAGTCTTCTTTTCGGCCTTTGGTGCCAGCCAGTTGGACCATTAGCTGCCTCTGTATTTGCGTGAAc                                                                                                                       >  2:277739/1‑239 (MQ=255)
                                                                                                       gAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTCCGTCTGTATGTGCGGGAACGGc                                                                                                                    >  1:671485/1‑239 (MQ=255)
                                                                                                         aaaaaGCG‑TTTGTTACCGCGCGTTTGATCAACTGGTCGTTGACTGACACTACTACGCGGTTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGt                                                                                                                  <  1:706520/237‑1 (MQ=255)
                                                                                                         aaaaaGCG‑GTTGTTACCGAGGGTTGTGTCAACTGGTCGTTGACTGACAGTTCTACGCGTCTGGTGAGCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCTGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACGGCGt                                                                                                                  <  1:826059/237‑1 (MQ=255)
                                                                                                             aGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGCGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGGGCCAGCACGTTGGCTCATGAGCTGTGTCTGTATGGGCCGGAACTGCGTGccc                                                                                                              >  1:36757/1‑239 (MQ=255)
                                                                                                              gCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATAAGCCGCGTCTGTATGTTCGTGAAATGCGTGACCg                                                                                                             >  1:103791/1‑239 (MQ=255)
                                                                                                                   ttGTTACCGAGCGTCTGCTCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCAGTCTCGGCGTGGCCTATGGCTCCGCTCTGGCAAAAGGGCGTAAAGTGTTACTCAAGGCGGCGACTGCGCACCC‑AAgg                                                                                                                                                                                                               >  1:212387/1‑137 (MQ=255)
                                                                                                                        aCCGGGCGTCTGACCAACTGGTCGTTGACTGACACTACTACGCGTCGGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                    <  1:219839/239‑1 (MQ=255)
                                                                                                                          cGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCga                                                                                                      >  1:62623/1‑234 (MQ=255)
                                                                                                                          cGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGt                                                                                                      >  1:976821/1‑235 (MQ=255)
                                                                                                                            aGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTGGGATAATGAGCTCGGTCTGTATGTGCGTGAACTGCGGGACCGTAGGCGTAGTgcg                                                                                                >  2:106745/1‑237 (MQ=255)
                                                                                                                              cGTCTGATCAGCTGGTCGGTGACTGACCCTACTGCGCCTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGGTACTGAAGGCGGCGACTGAGCTCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                                                                               <  1:832152/238‑1 (MQ=255)
                                                                                                                                  ttttCAACTGGGGGTGGACTGACACTACTACGGGGTTGGTGAGCCGTCGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                          <  2:573417/236‑1 (MQ=255)
                                                                                                                                  tGATCAACTGGTCGTTGACTGACACTACTACGGGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                          <  1:456265/239‑1 (MQ=255)
                                                                                                                                    ataaccTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCt                                                                                        <  2:52222/234‑1 (MQ=255)
                                                                                                                                      cacttGGTCGTTGACTGACACTGCTTCGCGGCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCACC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTGTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTCCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑g                                                                                      <  2:162215/234‑1 (MQ=255)
                                                                                                                                       aaCTGGTCGTTGACTGACGTTACTACGGGTCTGGTGATCCGTCACGGCGTGGCCTATGGCACCGATCTGGAAAAAGTGCGTAAAGTGTTAATGAAGGCGGCGACTGAGCACCG‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa                                                                                    <  1:507669/239‑1 (MQ=255)
                                                                                                                                       aaCTGGTCGGTGACTGACCATACTACGTGTCCGGTGATCCGTCTCGGCGTGGCCTGTGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa                                                                                    <  1:442352/239‑1 (MQ=255)
                                                                                                                                       aaCTGGGCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa                                                                                    <  1:446964/239‑1 (MQ=255)
                                                                                                                                              cGTTGACTGACCCTACTACGGGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTAc                                                                              <  2:874145/238‑1 (MQ=255)
                                                                                                                                              cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATCTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGAGACCGTAGTCGGACTGTCGATGAGCT‑GAACCGTACt                                                                             >  1:63016/1‑239 (MQ=255)
                                                                                                                                              cGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGTTGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTCCGTCTTTAGTGGCGTGAACTGCGTGACCGTGTTCGTACT‑TCGGTGAGCTGGAACCGAACt                                                                             >  2:640001/1‑239 (MQ=255)
                                                                                                                                                  gactgacCCTTCTACGGTTCTGGTGCTCCGTCTGGGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCg                                                                         <  2:671485/239‑1 (MQ=255)
                                                                                                                                                   gctgacACTCCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTa                                                                               <  1:102516/231‑1 (MQ=255)
                                                                                                                                                   actgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATTTTCGTGAACTGCGTGACCGTAGTCGTACTTTCGATGAGCT‑GCACCGGACTATCGa                                                                        >  1:545388/1‑239 (MQ=255)
                                                                                                                                                       gactctcctcgggttgggggATCCGTTCCGGCTGGGCCTAGGGCCCCGAATTGGAAAAAGGGCGTAAAATGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAg                                                                    <  2:719445/220‑1 (MQ=255)
                                                                                                                                                             ctacGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGGGCCAGCACGTTGGATCATGAGCTGCGTCTTTTTGGGCGTGAACTGCGTGACCGAAGTCGGCCTGTCGGTGAGCT‑GAACCGAACAATAGATAAGAtggc                                                               >  2:102516/1‑236 (MQ=255)
                                                                                                                                                                cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa                                                                                                                                                                                                                 <  2:491509/90‑1 (MQ=255)
                                                                                                                                                                cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑aa                                                                                                                                                                                                                 >  1:491509/1‑90 (MQ=255)
                                                                                                                                                                 gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGCAGTCTTCTTTACGGCATTTGGGGCCAGCACGTTGGATCATGAGCTGCGTCTGTCTGTGCGTAAACTGCTGGCCCGTAGACGGACTGTGGAGGGGCT‑GAACCGGCCTATCGATTACCTGGTCCGTg                                                          >  2:771480/1‑239 (MQ=255)
                                                                                                                                                                 gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                    >  1:378610/1‑198 (MQ=255)
                                                                                                                                                                 gcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                    <  2:378610/198‑1 (MQ=255)
                                                                                                                                                                   gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                                                                                                                                              <  2:483454/138‑1 (MQ=255)
                                                                                                                                                                   gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                                                                                                                                              >  1:483454/1‑138 (MQ=255)
                                                                                                                                                                     ttgtgtttCCGTGTCGGGGGGGCCTTGGGCTCCGATCTGGAAAAAGTGCGTAAAGGTTTTCTGAAGGCGGTGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa                                                      <  2:569309/232‑1 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTTGATCATGAGCTGCGTCTGTATGTGCGTGAACTGAGGGACCGTAGGCGTACTGTCGTTTGCTT‑CAACCGCACTATCCTTCAGCtttctgcgaaaaa                                                      >  2:686720/1‑227 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa                                                      >  1:498267/1‑239 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCGGTATGTGCGTGAACTGCGGGACCGTAGGCGTACTGTCGATGCGCT‑GAACCGGACTATCGaccctgtctctttaaaaa                                                       >  2:137030/1‑221 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑Gaa                                                                                    <  1:680088/209‑1 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGGTCAGCTGTGCCGTGaaaa                                                      >  1:146325/1‑239 (MQ=255)
                                                                                                                                                                     cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGGGaaa                                                       >  1:569309/1‑238 (MQ=255)
                                                                                                                                                                     atGGTGATCCGGTTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaa                                                       <  1:377748/237‑1 (MQ=255)
                                                                                                                                                                      ctgTGATCCGTCTCGGCGGGGCCTATGGCTTCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGaaaa                                                      <  1:931275/236‑1 (MQ=255)
                                                                                                                                                                       ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGTTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGGGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGGGCT‑GGACCGGAATATCGATCAGCTTTGCCGGGAAAAcc                                                    >  2:643996/1‑238 (MQ=255)
                                                                                                                                                                       ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                                                                                                                                             <  2:476255/135‑1 (MQ=255)
                                                                                                                                                                       ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAg                                                                                                                                                             >  1:476255/1‑135 (MQ=255)
                                                                                                                                                                             ccGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGACCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGCCCGGAGTCGACCTTGCGATGAGCT‑GAACCGAACTATACATCAGCTGGGCCGGGAAAACgcaataa                                              >  2:1031670/1‑233 (MQ=255)
                                                                                                                                                                                 ttctgCGGGGCCTTTGGCTCCGGTCTTGAAAAAGTGCTGAAAGTGTTATGGAAGGCGTGGACTTAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGGCGTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑TAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACAt                                         <  2:608442/235‑1 (MQ=255)
                                                                                                                                                                                 ctgggCGGGGGCTTTGGACCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACa                                          <  1:344215/235‑1 (MQ=255)
                                                                                                                                                                                  tcGGGGTGGCCTGTGGCGCCGATCGGGAAAAAGGGGGTAAAGGGGTACGGAAGGCGGCGACTGAGCACCA‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTGACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGGGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACAt                                         <  2:875019/238‑1 (MQ=255)
                                                                                                                                                                                   cGGCGTGGGCTTAGGCTTCGGTCGGGAAAAAGTGCGTAAAGTTTTAATGAAGGCGGGGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGGCGGAAGGTTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTg                                       <  1:413450/239‑1 (MQ=255)
                                                                                                                                                                                                     cGATCTGGAAAAAGTGGGTAAAGGGTTAATGAAGGCGGCGACTGAGCGCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAg                     <  1:640001/239‑1 (MQ=255)
                                                                                                                                                                                                     cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑ACGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACTTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTCACCGCAGTCGAACTTTCGGTGACCT‑GAACGGTCCTACCGGTAAGCCTTGCCGGGAAAACCACAATCAA‑ACTTCCTTTAACCAGCTTTAAg                     >  2:644682/1‑239 (MQ=255)
                                                                                                                                                                                                                aaGGGCGGAAAGTTTTACTGAAGGCGGGGACTGAGCACCC‑AAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac          <  2:482498/239‑1 (MQ=255)
                                                                                                                                                                                                                 aGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATTAGCT‑GAACCGAACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAAccctgccccttataaacatctcc         >  1:653688/1‑218 (MQ=255)
                                                                                                                                                                                                                      gTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTAACAGTAGTCGTACTGTCGATGAGCT‑GCACCGTACTATCGATCAGCTGGGCCGGGAAAACGAC‑ATCAACAAGGCCTTAAACCAACTTGAAGGGctccggaacaagaaa    >  2:179439/1‑225 (MQ=255)
                                                                                                                                                                                                                        aaaGTGTTACTGAAGGCGGCGACTGAGCCCCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGaa  <  1:404661/239‑1 (MQ=255)
                                                                                                                                                                                                                            |                                                                                                                                                                                                                                             
TTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCG‑TTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCC‑AAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCT‑GAACCGTACTATCGATCAGCTGTGCCGTGAAAACGAC‑ATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAA  >  NC_000913/489378‑489831

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A