Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,597 T→G 9.5% V1021G (GTG→GGG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,5970TG 9.5% 424.1 / 2.1 158V1021G (GTG→GGG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (59/77);  new base G (14/6);  total (75/83)
Fisher's exact test for biased strand distribution p-value = 3.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 159 total aligned reads displayed.

TCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAAG  >  NC_000913/489360‑489832
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
    aCGTCCGGTGCGTATGGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa                                                                                                                                                                                                                                          <  1:911421/238‑1 (MQ=255)
            tGCGTTTTGGTGTTACGGTAACCATTGGTAGCTTCTGGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAggc                                                                                                                                                                                                                                  <  2:716265/238‑1 (MQ=255)
                    ggCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                         <  1:494980/239‑1 (MQ=255)
                    ggCGATACCGTAACCAGTGGGAGCCTCTCGGGTACGGTAAGTAATGTCCGTATTCGTGCGACAACGATTACCGATTGCGATCGCAAAGAAGTGATCATCCCTAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                         <  1:514434/239‑1 (MQ=255)
                       attatgttAACCTTTGGTAGCTTCTCGGGGACGGTAATTAAAGTCCGTTTGCTGACCACAACGATTACCTGTTTCCATCGCAAGGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCTTCTGGTGATCCGTCTC‑GGCGTGGCCTCTGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGcc                                                                                                                                                                                                                      <  2:153497/232‑2 (MQ=255)
                        aTACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTCTTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGCCTGTTACACTGGTCGTTGCCTGACACTACTACGCTCTTGGTGTTCCTTCTC‑GCCGTGGCCTAGTGCCCCGTCCTGGTAAAATGGGTTAAAGGGTTCCTTAAGGCGGCGAGAGAGCa                                                                                                                                                                                                                      >  2:375816/1‑238 (MQ=255)
                         tatggTAACCGTTTGTGGCTTCTCGGGGGCGGTAAATAAGATACGTGTTCGTGCGAACACGGTTGCCGATTTGGATCGAAAAGAAGTGATCATCCAGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGGCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                                                                                                                                                    <  2:168941/236‑1 (MQ=255)
                         atcggtacctttttgTAGCTTTCCGGGGACGGTAAGTAAGAACCGTATTCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAcc                                                                                                                                                                                                                    <  2:935474/225‑1 (MQ=255)
                          aCGGTAACCTTTGGTGGCTTCTCGGGGAAGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc                                                                                                                                                                                                                   <  2:727090/239‑1 (MQ=255)
                          aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc                                                                                                                                                                                                                   >  1:1043149/1‑239 (MQ=255)
                          aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAg                                                                                                                                                                                                                        >  1:956317/1‑234 (MQ=255)
                           cGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCa                                                                                                                                                                                                                  <  1:835864/239‑1 (MQ=255)
                               aaCCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGTTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGTGCCTCGGCACCCCA‑ggg                                                                                                                                                                                                             >  1:119275/1‑239 (MQ=255)
                                     tGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGAGTTGGAAAAAGTGCGGAACACGTTACTGACGGCGGGCACGGATAACCCAA‑GGGGGagg                                                                                                                                                                                                        >  2:75875/1‑236 (MQ=255)
                                      ggTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGCTCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCCAA‑GGGGAATGc                                                                                                                                                                                                       >  1:977777/1‑238 (MQ=255)
                                            ttCTCGGGGACGGTAAGTAAAGTCCTGTTTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGAGCATCCCGAACAAAGGTTTTGGTCCCGAGCGTCTGTTCAACTGGTCGTTGACTGACTCTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAAcc                                                                                                                                                                                                <  2:822407/239‑1 (MQ=255)
                                                 ggggACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCCAA‑GGGCGATGCACGAACCAATg                                                                                                                                                                                            >  1:902420/1‑238 (MQ=255)
                                                     aCGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGAAAAGTGTTACTGCAGGCGGCGACTGAGCACCCAA‑GGGTGAGGCACAACCAAAGGCCGGa                                                                                                                                                                                       >  2:496472/1‑239 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGCTCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTGACCTGAACCTACTACGCGGCTGGTGATCCGTCTC‑GGCGTGGCCAATGGCTCCTCACTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGCGAAGGGGCACCCAA‑GGGTGATCGACGAACCaccacaggaa                                                                                                                                                                                      >  2:892299/1‑230 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGc                                                                                                                                                                                                       >  1:46591/1‑222 (MQ=255)
                                                      cGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGCCTGAGCACCCAA‑GGGTGATGCACGACCCAATGCCGGaa                                                                                                                                                                                      >  1:64445/1‑239 (MQ=255)
                                                           agtaagATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGCCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCCCCGATCTGGACAAAGGGCGTAAAGGGTTAATGGAGGGGGGGACTGAGCCCCCCA‑GGGAGATGACAGAACCaagccccgagagcct                                                                                                                                                                                 >  2:234948/1‑226 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTa                                                                                                                                                                                                                                          <  2:111082/177‑1 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAAGTCCGGACGTATTCTTTAc                                                                                                                                                                           >  1:657290/1‑239 (MQ=255)
                                                                 aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCAGGAACCAATGCCGGAAgcgtgttttac                                                                                                                                                                           >  1:146983/1‑229 (MQ=255)
                                                                  tCCGTATTCGTGCGACAACGATTACCGATTCCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTa                                                                                                                                                                            <  1:881044/237‑1 (MQ=255)
                                                                   ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGCCTGAGCACCCAA‑GGGGGAGTCACGAACCAATGCCGGAAGCcttctt                                                                                                                                                                              >  1:617761/1‑234 (MQ=255)
                                                                   ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGGCTTCTTTACg                                                                                                                                                                          >  1:1002012/1‑238 (MQ=255)
                                                                      tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAggcgg                                                                                                                                                                                                                                <  1:271393/182‑1 (MQ=255)
                                                                      tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTCACTGAAGGCGGCGACTGGGCACCCAA‑GGGTGATGCCCGAACCAATCCCGGAAGGGTTTTTTAGGGAAt                                                                                                                                                                      >  2:606575/1‑239 (MQ=255)
                                                                            tGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGGAAAGGGTTACTGAAGGCGGCGCCTGGGCACCCAA‑GGGGGGTGCACGAACCAAACCCGGAAGGCTTCTTTACGGGATTggggg                                                                                                                                                                >  2:1038714/1‑237 (MQ=255)
                                                                                aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGCGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAATGTGGTACTGCAGGCGGGGCCTGAACCCCCAACGGGTGTAGCAGGAACC‑ATCAAGGAAGACTTATTTACGGCAAATGGTGCCa                                                                                                                                                             >  2:267029/1‑238 (MQ=255)
                                                                                       ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                      <  1:75875/238‑1 (MQ=255)
                                                                                       ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTg                                                                                                                                                     >  1:619528/1‑239 (MQ=255)
                                                                                              tttCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTTAGCACCCAA‑GGGTGAt                                                                                                                                                                                                         >  1:869142/1‑180 (MQ=255)
                                                                                               ttCGATCGCACAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTC‑GGCGTGGCCTATTGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAGGGCGGGGGCTGAGCACCCAA‑GGGTGATGCACGAGCCAAGGCCGGAAGCCTTCTTTAAGCCATTTGGTGAAAGCAAGGTgggcaatgg                                                                                                                                             >  2:260708/1‑232 (MQ=255)
                                                                                                tcggtcacaaaaaattgtctatcCCGCACAAGGCTTTTTCTCGCGAGCGTCGGAACGACCGGGCGGGGTCTGACAATACTACGGGTCAGGTGATCCTGCGC‑GGGGGGGCCGATGGCTAGGATCGGGAAAAAGTGCGTAAAGTGTTGGTGAAGGGGGAGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGCATCATggg                                                                                                                                            <  2:815028/219‑3 (MQ=255)
                                                                                                tCGATCGCAAAGAAGTGATCCTCCCGAACAAAGCGTTTGTTACCGAGCGTCTGAACAACTGGTCGTTGACTGGCACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTTCGTAAAGTTTTCCTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:781104/239‑1 (MQ=255)
                                                                                                tCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:446224/239‑1 (MQ=255)
                                                                                                 cGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGc                                                                                                                                           <  1:223822/239‑1 (MQ=255)
                                                                                                  gtgcGCAAAGAATTGATCATCCCGAACAAAGCGTTTTTTACCGTGCGTCTGAACAACGGGTCGTTGACTGACACTACGACGCGGCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGGAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  2:902420/236‑1 (MQ=255)
                                                                                                  gacagCAAAGAAGTTATTACCCCGAACGAAGCTTTTGTTTCCGGGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGATTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  2:480893/235‑1 (MQ=255)
                                                                                                  gATCGGAAAGAAGTGATCAGCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:428958/239‑1 (MQ=255)
                                                                                                  gATCGGAAAGAAGTGATCAGCCCGAACAAAGCGTTTGGTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:234948/239‑1 (MQ=255)
                                                                                                   atgtttataaggtgaagatcCCGAACAAAGCTTTTGTTTCCGAGCGTCTGATCCACTGGTCGTTGCCTGACACGACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:702821/221‑1 (MQ=255)
                                                                                                            aagTGATCCTCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:67117/229‑1 (MQ=255)
                                                                                                             attGATCAGCCCGAACAAAGCGTTTGTTTCCGAGGGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGGTCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCAACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                                 <  1:89987/235‑1 (MQ=255)
                                                                                                                   ctcccctgaCAAAGCTTTTTTTTCCGAGGGCATGATCAGTGGGTCGTTGACTGACCCTACTAGGGGTCTGGTGATCCGGCTG‑GGGGGGGCCTTTGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTg                                                                                                                         <  2:562179/231‑1 (MQ=255)
                                                                                                                     tcCCGAACAAAGCGTTTGTTCCCGAGCGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  1:25617/218‑1 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGGGCACCCAA‑GGGCAATGCACGAACCAATGCCGGAGGTCTTATTTACGGCCTTTGGGGCCACCACGTGGGACAAGCAGCGGGGTCTGTATGTGGGGGaaa                                                                                                                      >  2:452351/1‑238 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATTTGCGTGAAc                                                                                                                      >  1:953548/1‑239 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGACCCAATGCCGGAAGCCTTTTTTACGGCCTTTGGTGCCAGCACGTTGGGCCAGGAGcgccgcctggtgttgctggacc                                                                                                                      >  1:562179/1‑218 (MQ=255)
                                                                                                                      cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTC‑GACGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGCCTTCTTTACGCCATTTGAGGCCAGCACGTTGGATAATGAGCtcggctggaatccggatcctt                                                                                                                      >  2:67117/1‑219 (MQ=255)
                                                                                                                         gAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGCCTCCGATCTGGAAAAAGTGCGCAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGAGGCTGTATGTGCGTGAACTGc                                                                                                                   >  1:301187/1‑239 (MQ=255)
                                                                                                                               aGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAC‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGACCGTTGGGCCATGAGCTGCGTCTGTATGGGAGTGAACGGCGTGAcc                                                                                                             >  1:294195/1‑239 (MQ=255)
                                                                                                                                  ttttGTTACCGGGGGTGTTGTCAAGTTGTCGTTGACTGACACGACTACGCGTTTGGTTATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg                                                                                                            <  2:64445/236‑1 (MQ=255)
                                                                                                                                         accggggttCTGATCAGCTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                   <  1:1068218/231‑1 (MQ=255)
                                                                                                                                         aCCGGGCGTCGGATCAACTTGTCGTTGACTGACCCTACTTCGCGTCTGGTGAGCCGTCTC‑GGCGTAGCCTATGGCCCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                   <  1:361794/239‑1 (MQ=255)
                                                                                                                                                   ttgttaaCTGGTGTTTGGCTGACCCTACTCCGCGTCTGGTGATCCGGCCC‑GGCTGGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  2:1049699/234‑1 (MQ=255)
                                                                                                                                                   tggtCAACTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  1:590374/236‑1 (MQ=255)
                                                                                                                                                   tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAg                                                                                         <  1:496472/239‑1 (MQ=255)
                                                                                                                                                      ttctttggtcttttatttcctctattaCGGGTTTGGTGTTCCGTCTC‑GGCGTGGCCTTTGGTGCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGTATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg                                                                                      <  2:53675/218‑1 (MQ=255)
                                                                                                                                                               cGTTGACTGACACTACTACGCGACTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGAGCCCGCACGTCGGATCATGAGATGCGTCTGTATGTGCGTGAACTGCGGGACCGTAGTCGTACTGTCGATGAGCGGAACCggcgt                                                                             >  2:222780/1‑235 (MQ=255)
                                                                                                                                                                  tgactgacCCTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            <  2:390940/173‑1 (MQ=255)
                                                                                                                                                                  tgactgacACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                            >  1:390940/1‑173 (MQ=255)
                                                                                                                                                                   ggttgacctttttacGCGTTTGGTGTTCCG‑CTCGGGCGTGGCCTAGTGCTTCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTTAGCACCTAA‑GGGTTATGCACGAACCAAGGCCGGAAGTCTTCTTTACGGGATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                                                                         <  2:977777/227‑1 (MQ=255)
                                                                                                                                                                    actgacACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCa                                                                                                                                                                                                      <  2:90795/113‑1 (MQ=255)
                                                                                                                                                                                    gTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCCCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGGAGGCGTACTGGCGATGAGCTGAACCCTCCTACCgcacaccttgaccgtgaa                                                        >  2:242981/1‑222 (MQ=255)
                                                                                                                                                                                      cTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGCTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCCTTTGGTGCCAGCACGTTGGATCTGGAGCTGCGTCTGGATGTGCGTGAACTCCGTGACCGAAGCCGTACTGTCGGTGGGCTGAACCGTGCTATCGATCAGCTGTGCCGttcaaa                                                      >  2:1043821/1‑234 (MQ=255)
                                                                                                                                                                                      cTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATAAGCTGTGCCGTGaaa                                                       >  1:756219/1‑238 (MQ=255)
                                                                                                                                                                                       tGGGGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAc                                                     <  1:168249/239‑1 (MQ=255)
                                                                                                                                                                                            aTCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGGTCATGGGCTGCGTCTGGATGTGCGTGAACTGCGTGCCCGGAGGCGTACTGTCGATGAGCTGAACCGCACTACTGTTCACCTGTGCCGGGAAAAcaaaac                                                >  2:1078985/1‑234 (MQ=255)
                                                                                                                                                                                                  ctc‑GGCGTGGCCTATGGCTCCGATCGGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCAAGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:1043821/239‑1 (MQ=255)
                                                                                                                                                                                                   tggggctgggCCTATGGCTCCGTTTTGGAAAAAGGGCGTAAAGTTTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  2:584032/231‑1 (MQ=255)
                                                                                                                                                                                                   tc‑GGGGTGGCCTATGGCTCCGAACTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:397951/238‑1 (MQ=255)
                                                                                                                                                                                                   tc‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          >  2:27120/1‑142 (MQ=255)
                                                                                                                                                                                                   tc‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                          <  1:27120/142‑1 (MQ=255)
                                                                                                                                                                                                   tc‑GGCGGGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  1:116127/238‑1 (MQ=255)
                                                                                                                                                                                                    cggggggggcggagggCGCCGAACTGGAAAAAGGGCGGAAAGGGTTACTGAAGGCGGGGCCTGACCACCCAA‑GGGTGATGCCCGAACCAATGCCGGAAGGCGTCTTGACGGAATTTGGTGCCAGCACGTGGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                          <  2:713783/224‑1 (MQ=255)
                                                                                                                                                                                                            gCCTATGGCTTCGATCTGGAAAAAGTCCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCGATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa                                 <  1:19810/239‑1 (MQ=255)
                                                                                                                                                                                                            gCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGGGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAATATCGATCAGCTTGGCCGGGAAAACGAAATCAACAATGCCTTTaa                                 >  2:610340/1‑239 (MQ=255)
                                                                                                                                                                                                             gctgtgggcccGATTTGGAAAAAGTGGGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTaa                                 <  2:953548/229‑1 (MQ=255)
                                                                                                                                                                                                                     tCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTg                         <  1:1078985/238‑1 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCACCATTGCCTTTAACCAGCTTGaa                       >  1:45336/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGaa                       >  1:796559/1‑239 (MQ=255)
                                                                                                                                                                                                                      ccGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATGGCCTTTAACCAGCTTGaa                       >  1:538818/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGTTGAACCGTACTATCg                                                                         >  2:15226/1‑188 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                                                                         <  1:15226/188‑1 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGTTCACCTGTGCCGTGAAACCGCAATCAAAATTGCCTTTAACCACCTTGaaa                      >  1:107207/1‑238 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTACCGCTCAGCTGGGCCGGGAAAACCACCTCAACATTGCCTTTAACAAGCTTAAAg                      >  2:813470/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGGGCGTGAACTGCGTGACCGTATTCGCACTGTCGATGAGCTGAACCGTGCTATCGATCATCTGGGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCCTGAAg                      >  2:178723/1‑239 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGAATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAg                      <  1:178723/239‑1 (MQ=255)
                                                                                                                                                                                                                       cGATCTGGAAAAAGTGCGTAAAGAGTTACTGAAGGCGGCGACTGA‑CACCCAC‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGAATGTGCGTGAACTGCGTGACCGTCGTCGTACTGTCGCTGAGCTGAACCGCACTATCGATCAGCTGTGCCGTGAAAACGCACTCAACATTGCCTTTAACAAGCTTGAAGt                     >  2:43126/1‑239 (MQ=255)
                                                                                                                                                                                                                         aTCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGTTCAGCTGTGCCGTGAAAACGCCATCAACATTCCCTTTAACCAGCTTGAAgg                     >  2:358636/1‑237 (MQ=255)
                                                                                                                                                                                                                                aaaaGTGCGTAAAGGGTTACGGAAGGCGGCCACTGGGCCCCCAA‑GGGGTATGGCCGAACCAATGCCGGAAGTCTTTGTTCCGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGAGTCTGTATGTGCGTTAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGc             <  2:619528/239‑1 (MQ=255)
                                                                                                                                                                                                                                  aaggtctataaGGGTTACTGGAGGTGGGCTATGAGCACCCAA‑GGGTGATGCACGAACCAAGTCCGGAAGTTTTCTTTACGGTATTTGGTGCCAGCGGGTTGGATCATGAGCTGTGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGCCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac           <  2:77140/230‑1 (MQ=255)
                                                                                                                                                                                                                                  aaGTGCGTAAAGTGTTACGGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGcac           <  1:768569/239‑1 (MQ=255)
                                                                                                                                                                                                                                        gTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCTGGAAAACGACATAAACATTGCCTTTAACAAGCTTGAAGGGAATcggaaaaagtaa     >  2:100125/1‑228 (MQ=255)
                                                                                                                                                                                                                                          aaaGTGTTACTGAAGGGGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGaa   <  1:100125/239‑1 (MQ=255)
                                                                                                                                                                                                                                            aGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTGGTCGTACTGTCGCTGAGCTGAACCGTCGTATCGATCAGCTGTGCCGTGAAAACGACACCAACATTGCCTCTAACACGCTTGAATGGCATCTgtccattataaaa  >  2:83975/1‑226 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                            
TCGAACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAAGATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTC‑GGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAA‑GGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAGAAG  >  NC_000913/489360‑489832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A