Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F9 I0 R1 142 103.7 2603848 81.8% 2129947 227.3

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 489,597 T→G 12.4% V1021G (GTG→GGG)  mscK → mechanosensitive channel protein, intermediate conductance, K+ regulated

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913489,5970TG12.4% 334.1 / 8.8 136V1021G (GTG→GGG) mscKmechanosensitive channel protein, intermediate conductance, K+ regulated
Reads supporting (aligned to +/- strand):  ref base T (50/61);  new base G (15/3);  total (70/66)
Fisher's exact test for biased strand distribution p-value = 4.12e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 137 total aligned reads displayed.

ACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAG  >  NC_000913/489364‑489829
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
   tCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGa                                                                                                                                                                                                                                  >  2:156117/1‑239 (MQ=255)
   tCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGCTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTCCTACGCGTCTGGTGATCCGTCTCGGCGTGGCCCATGGCTCCAATCTGGGAAAATGTCGCAAAGTGTTACtca                                                                                                                                                                                                                                  >  2:386183/1‑237 (MQ=255)
            tATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCTTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTTAAGgcggcg                                                                                                                                                                                                                          >  2:728692/1‑238 (MQ=255)
              ttGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACt                                                                                                                                                                                                                       <  2:262361/239‑1 (MQ=255)
                ggcctttgtgttccctttGGTAGCTTTTGGGGCAGGGTAAGTCA‑GAGCTGTATTCGGGCGACAACGATTACCGATTTCGACCTCAAAGAAGTGATCCTCCCGACCAAAGCGTTTGTTACCGAGCGTCTTATCAACTGGTCGTTGACTGACACTTCTACGCGTCTGGTGATCCGTGTCGTCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTCCTGAAGGAGGCGACTGa                                                                                                                                                                                                                     <  1:1091666/223‑1 (MQ=255)
                    aTACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGCTCAACTGGTCGTTGACTGACACTACTACGGCTATGGGAATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGCAAAGGGACGAAAGGGGTTACTGAGagcggcacctaagct                                                                                                                                                                                                                  >  1:388378/1‑229 (MQ=255)
                      aCGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAccc                                                                                                                                                                                                               <  2:1246261/239‑1 (MQ=255)
                            aCCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGAGTTACTGAAGGCGGCGACTGAGCACCCAAgggg                                                                                                                                                                                                         >  2:347689/1‑238 (MQ=255)
                             ccATTGGTTGGTTCCGGGGGAACGTAAGGAAGGATCGGTGTCCGT‑CGAAAACGATTGCCCATTTCGATCGGAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTg                                                                                                                                                                                                        <  1:1032758/239‑1 (MQ=255)
                               aTTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCAAAGGGTGAt                                                                                                                                                                                                      >  2:143606/1‑239 (MQ=255)
                                  ggTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCa                                                                                                                                                                                                   >  2:1139695/1‑239 (MQ=255)
                                  ggTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGGGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAACGCGTCAAGGGTTACTGAAAGGGGCGACTAAGCACCCACgggggcacca                                                                                                                                                                                                   >  1:156283/1‑232 (MQ=255)
                                  ggTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTAGTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCa                                                                                                                                                                                                   <  2:720024/239‑1 (MQ=255)
                                    tAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCCCg                                                                                                                                                                                                 >  2:1262628/1‑239 (MQ=255)
                                       cTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGACCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGGGACTGAGCACCCAAGGGGGATGCACGAAc                                                                                                                                                                                              >  2:230472/1‑239 (MQ=255)
                                        ttCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGACCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGGGGGGACTGCGCACCCAAGGGGGATgacagaacc                                                                                                                                                                                             >  2:524417/1‑231 (MQ=255)
                                                 acagtaagtaa‑TATCCGTATCCGAGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGAGCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATAAACTGGTCGTTGACTGACACGACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCGCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGcctg                                                                                                                                                                                     <  2:1160484/235‑3 (MQ=255)
                                                 aCGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCgg                                                                                                                                                                                     <  2:647416/238‑1 (MQ=255)
                                                    gtaagtaa‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGCCTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTCTGGCCCCGCTCTGGAAAAAGCGCGTAAAGAGTTACTGAAGGCGGCGCCTGAGCACACAAGGGGGAGGCACGACACAATGCCGGAAGt                                                                                                                                                                                 >  1:708154/1‑239 (MQ=255)
                                                    gtaagtaa‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGCCTGACACTACTACCCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGAGCGTAAAGTGTTACTGAAGGCGGCGACGTAGCACCCAAGGGGGAGGCACGACCAAATGCCGGGAg                                                                                                                                                                                  >  1:1293488/1‑238 (MQ=255)
                                                    gtaagtaa‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAgg                                                                                                                                                                                 >  2:852526/1‑238 (MQ=255)
                                                     taagtaa‑gATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGt                                                                                                                                                                                 <  2:597679/238‑1 (MQ=255)
                                                           a‑gATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCttt                                                                                                                                                                          <  2:388378/239‑1 (MQ=255)
                                                              aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATTTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAGGTCTCCTTTAc                                                                                                                                                                        >  2:195886/1‑239 (MQ=255)
                                                              aTCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTTAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGACTTCTTTAc                                                                                                                                                                        >  2:802782/1‑239 (MQ=255)
                                                                ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACg                                                                                                                                                                       >  2:1198443/1‑238 (MQ=255)
                                                                ccGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCCCGCACCAATTCCGGGAGTCTTCTTTACg                                                                                                                                                                       >  2:743201/1‑238 (MQ=255)
                                                                   tATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGGGGCCTATGGCTCCCACCTGGAAAAAGTGCGTAAATTTTTACTGAAGGCGGCGACTGAGCACCCACGGGTGAGGCCCAAACCAAAGCCGGAAGTCTTTTTTACgccct                                                                                                                                                                   >  1:1086523/1‑235 (MQ=255)
                                                                     ttCGTGCGAAAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGTGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCAttt                                                                                                                                                                 <  2:86617/239‑1 (MQ=255)
                                                                             aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCCCGAACCAATACCGGAAGGCTTCTTTACGGCCTTTGGTGccg                                                                                                                                                          >  2:1079196/1‑237 (MQ=255)
                                                                             aCAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTAACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGCAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCa                                                                                                                                                          >  2:100840/1‑238 (MQ=255)
                                                                               aaCGATTACCGATTTCGATCGCAACGAATTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCCGGTGATCCGTCTCGGCGTGGCCAATTGCTCCATTCTTGAAAAAGTGCGTACAGTGCTACTGAAGCCGGCGAAGCGGCAACCCACGGCGATGCAAGAACCAATGCCGGCATTCTTCTTTAGGCCAATTGGAGCCAGcc                                                                                                                                                       >  1:961191/1‑238 (MQ=255)
                                                                                    tttccgtattCGAGCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGGGCGTCTGATCAACTGGTCGTTGACTGACACGACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                   <  2:348722/230‑1 (MQ=255)
                                                                                    ttACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                   <  2:224371/238‑1 (MQ=255)
                                                                                          aTTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGa                                                                                                                                                                                                                     >  1:5420/1‑166 (MQ=255)
                                                                                          aTTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACTAACCAATGCCGGAAGGCTTCTTTACGGCCTTTGGTGCCAGCACGTTGGAt                                                                                                                                               >  2:771411/1‑236 (MQ=255)
                                                                                             tccgaccaaaagaatgtgtcacaccGAACAAAGCGTTTGTTAACGGACGTCTGATCAACTGGGCGGTGAGTGGCACCACTTCGCTGCTGGTGATCCGTCCCGGGGGGGCCTTGGGCTGCGATCTGGAAAAAGTGGGTAAAGTGTTACTGAAGGCGGGGAATGATCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGTATTGGGTGCCAGCGCGTTGGATCATGAg                                                                                                                                         <  1:1262628/216‑1 (MQ=255)
                                                                                             tCGATCGCAAAGAAGTGATCCTCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                         <  2:708154/239‑1 (MQ=255)
                                                                                               tatctcgatgaagggtggaacccAAACAAAGCGTTTGTTCCTGAGGGTCGTGTCATCGAGTGGTTGACTGAAAATACTACGCGTATGGTGAGCCGGCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       <  1:824547/219‑1 (MQ=255)
                                                                                               ggtCGTAAAGTAGTGATCATCCCTAACAAGGCTTTTGTTACCGAGCTTCTGATCCACTGGTCGTTGACTGACCCTACTGCGCGTGTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGAGCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCGTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       <  1:195886/237‑1 (MQ=255)
                                                                                               gATCGCAATGAATTGTTTTGCCCGACAAAGGTGTTTGTTACGGGGAGTCTTATCAACGGGTCGTTGTCTGACAATACTACGCGTCGGGTGATCCGGCTCGGAGTGGCCTTGGGCTCTGAGCTGGAAAAAGTGCGTAAAGTGTTACCGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       <  1:30925/239‑1 (MQ=255)
                                                                                               gATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       <  2:1086523/239‑1 (MQ=255)
                                                                                                 tCGCAAAGAAGTTATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGc                                                                                                                                     <  2:629402/239‑1 (MQ=255)
                                                                                                   gaaaaGAAGTGCCCCTCCCGAACAAAGGGTTTGTTACCAGGGGTCTGATCAACTGGTCGTTGACTGACACTACGACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGAGTTACTGAAGGAGGCGATTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGt                                                                                                                                   <  1:396200/237‑1 (MQ=255)
                                                                                                        tcagtttacaccccAAACAAAGCGTTTTTTGTCGAGCAGCTGATCAACTGGTCGTTCACTTGCTCTACTACGCGTCGGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCTGCGACTTAGCACCCAAGGGTGATGCACGAACCAATACCGGAAGTCTTCTTTACGGCAGTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTCTa                                                                                                                              <  1:820941/228‑1 (MQ=255)
                                                                                                        catgtggttctcCCGAACAAAGGTTTTTTTTCCGGGCGTCTTATCAAGTGGTCGTTGACGGACACTACTACGCGTCTGGTGATCCGTCTGGGCGTGGCCTAGGGCTCCGATCTGGAAAAAGGGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                              <  1:242288/229‑1 (MQ=255)
                                                                                                          agTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTa                                                                                                                              <  2:321808/237‑1 (MQ=255)
                                                                                                            ggATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTAGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTAGCCTATGGCTCCGATCTGGAAAAAGTGCGCAAAGAGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGaa                                                                                                                                                                                               >  2:1019566/2‑170 (MQ=255)
                                                                                                               tcatcCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGCCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGGGCTGGGTCTGTATGTGCGt                                                                                                                       >  2:242288/1‑239 (MQ=255)
                                                                                                                   cccGAACAAAGCGTTTGTTACCGATCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGaa                                                                                                                                                                                   <  1:815689/175‑1 (MQ=255)
                                                                                                                   cccGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGGCTTCTTTACGGGCTTTGGTGCCAGCCCTTTGGTTCATGAGCGGCGTCTGTATGTGCggggac                                                                                                                   >  2:581421/1‑234 (MQ=255)
                                                                                                                               gTTTGTTACCGAGCTTTTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCg                                                                                                         <  2:1293488/237‑1 (MQ=255)
                                                                                                                                  tGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAAcctgtctcttttacca                                                                                                    >  2:435394/1‑224 (MQ=255)
                                                                                                                                   gTTACCGGGCGTCTGATCAACTGGTCGTTGACGGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCATCTGTATGTGCGTGAACTGCGTGACCGTAGTCg                                                                                                   <  2:961191/239‑1 (MQ=255)
                                                                                                                                      aCCGGGCGTCTGATCCACTGGTCGTTGACTGACACTACTTCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                <  2:272013/239‑1 (MQ=255)
                                                                                                                                      aCCGAGCGTCTGATCAACTGGTCGTTTACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTAc                                                                                                <  2:412911/239‑1 (MQ=255)
                                                                                                                                          aGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCTGGAACTGCGTGACCGTAGTCGTACTGTc                                                                                            >  2:16738/1‑239 (MQ=255)
                                                                                                                                            tgttcgATCAACTTGGCGGTGACTGACACTACTGCGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                                                                           <  2:162613/233‑1 (MQ=255)
                                                                                                                                            cGTCTGATCAACTGGTCGTTGACTGACCCTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCg                                                                                           <  2:623279/238‑1 (MQ=255)
                                                                                                                                            cGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGGCGTACTTTCGa                                                                                          >  2:236577/1‑239 (MQ=255)
                                                                                                                                              tCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGGCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGGGAACTGCGGGACCGTAGTCGGACTGTCGATg                                                                                        >  2:1133997/1‑239 (MQ=255)
                                                                                                                                                tGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                         <  2:471854/188‑1 (MQ=255)
                                                                                                                                                    caccTGGTCGTTGACTTCCCCTACTACGGGTTTGGGTATCCGTCTCGGGGTGGGCTATGGCCCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTg                                                                                   <  1:1139695/235‑1 (MQ=255)
                                                                                                                                                         ggTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGCACTGTCGATGAGCTGAACCga                                                                             >  2:702666/1‑238 (MQ=255)
                                                                                                                                                          gTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATAAGCTGAACCGt                                                                             >  2:129326/1‑238 (MQ=255)
                                                                                                                                                           ctgTTGACTGGCACGAGTACGCGTCTGGTCAGCGGTCTCGGCGTGGCCTATTGCGTCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTa                                                                            <  2:626757/236‑1 (MQ=255)
                                                                                                                                                            ctttttcttccacTATTACTCTGCTGGTTATCCGTCCCGGCGTGGCCTATGGCTCCGAGCGTGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGAATTAGCACCCAAGGGTGATGCACGAACCAATGTCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGGGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAc                                                                           <  1:524417/228‑1 (MQ=255)
                                                                                                                                                            cgtttcctgacCCTACTGCGGTTCTGGTGACCCGGTGCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCCCCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTAc                                                                           <  1:247418/232‑1 (MQ=255)
                                                                                                                                                                gtctggccccgttcagcgtagggTGAGCCGGCGCGGGGTGTCCTATGGCTCAGACCTGGAAAAAGCGCGTAAAGTGTTACGGAAGGCGGGGTCTGAACACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCTCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCg                                                                      <  1:1157157/218‑1 (MQ=255)
                                                                                                                                                                 actgacACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGa                                                                     >  2:247418/1‑239 (MQ=255)
                                                                                                                                                                     acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       <  1:1034815/169‑1 (MQ=255)
                                                                                                                                                                     acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCt                                                                                                                                       >  2:1034815/1‑169 (MQ=255)
                                                                                                                                                                     acacTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCCATGCCGGAAGTCTTCTTTACAGCCCTTGTGGCACGCCCGTTGGAGCATGAGCTGCTGCTTTATGGGCGGGAACTGCGTGACCGGAGTGGGCCTGTCGTTAGGGTGAAGCGTAccaacgtatac                                                                 >  1:963080/1‑229 (MQ=255)
                                                                                                                                                                              cgcgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGCAGTATTCTTTACGGCATTTGGTGCCAGCCCGTTGATTCTTGTGCGTCGTCTGTATGTGCGTGAACTGCGTGCCCGAAGTCTGACTGTCGACGAACTGAACAGGAGTATCCAAAAGCTGGGcggg                                                        >  1:627108/1‑236 (MQ=255)
                                                                                                                                                                                cgTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGGGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGGACTACGGATCAGCTGTGCCGTGa                                                      >  2:482309/1‑239 (MQ=255)
                                                                                                                                                                                 gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                   <  2:4630/145‑1 (MQ=255)
                                                                                                                                                                                 gTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGtt                                                                                                                                                   >  1:4630/1‑145 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCa                                                                                                                                                                                                              >  1:1014962/1‑84 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGACGTCTTCTTTACGGCATTTGGTGCCACCACGTTGGACCATGAGATGCGTCGGTATGTGCGTGGACTGCGTGACCGTTGTCGTACTGTAGCTGAGCTGAACCGTACTATCTAGCAGCTGTGACGTGaaa                                                    >  1:1288900/1‑238 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAg                                                                 >  2:486617/1‑225 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa                                                   <  2:941699/239‑1 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACGGCGTGACCGTATTCGTACTGTCGATGAGCTGAACCGTACTATCAATCAGCTGGGCCGGGaaaa                                                   >  1:304817/1‑239 (MQ=255)
                                                                                                                                                                                   cTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGCGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGGGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAAGTACGTGCCAGGAGTCGTACTGTGGCTGAGCTGAACCGTCCTATCGATAAGCTGTGCGGAGaaaa                                                   >  1:520654/1‑239 (MQ=255)
                                                                                                                                                                                   cTGGTGACCCTGTTCTGCGTGGGTTGTGGCTCCGAGCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCAACCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGaaaa                                                   <  1:230472/239‑1 (MQ=255)
                                                                                                                                                                                    tGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAc                                                  <  2:304817/239‑1 (MQ=255)
                                                                                                                                                                                    tGGTGATCCATCGCGGGGTGGCCTGTGGCACCGATGTGGAAAATGTGCGTAAAGCGGTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCGGCCCGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTTACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAc                                                  <  1:453431/239‑1 (MQ=255)
                                                                                                                                                                                     ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGGGCTGAACCGTACTATCGATCAGCTGGGCCGGGAAACCg                                                 >  2:240235/1‑239 (MQ=255)
                                                                                                                                                                                     ggTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTCCTATCGATCACCTGTGCCGTGAAAACg                                                 >  2:854474/1‑239 (MQ=255)
                                                                                                                                                                                         aTCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACAt                                             <  2:156283/239‑1 (MQ=255)
                                                                                                                                                                                                tcGGCGTTGCCTATGGCTCCGATCTGGAAAAGGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACAt                                       <  2:64822/238‑1 (MQ=255)
                                                                                                                                                                                                tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                         >  1:374728/1‑140 (MQ=255)
                                                                                                                                                                                                tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAg                                                                                                                                         <  2:374728/140‑1 (MQ=255)
                                                                                                                                                                                                tcGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGCCATCAACAtt                                      >  2:339017/1‑239 (MQ=255)
                                                                                                                                                                                                    cGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGCCATCACCATTGCCt                                  >  2:796949/1‑239 (MQ=255)
                                                                                                                                                                                                                   cggtCTAGAAAAAGGTCGTAAAGGGTTACTGAAGGCGGCAACTGGGCACCCAAGGGTGAAGCACGAACCAATGGCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAg                   <  2:1077124/236‑1 (MQ=255)
                                                                                                                                                                                                                   cGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAAGGACATCAACATTGCCTTTAACCAGCTTGaac                   >  2:467936/1‑238 (MQ=255)
                                                                                                                                                                                                                            aaaaGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTg           <  2:543928/238‑1 (MQ=255)
                                                                                                                                                                                                                              aaGTGCGTAAGGGGTTAGAGAGGGGCGCTCCGGAGCACCCCAGAGTGACGACCGAAACAATGACGGAATGCGTGTGTACGGCATTAGGTGCCAGCAGGTGAGATCATGATGTGCGTCTGTATCGGCGTGAACTGCGTGACCGGAGTCGGAGTGTCGATGAGCTGAACCGTACTAGCGATGAGCTGTGCCGTGAAAACGACATCAACATAGACTTTAACCTGCTTGAATTGCATCTGcac        <  1:44474/239‑1 (MQ=255)
                                                                                                                                                                                                                                    ggaaggtgtTACTGAAGGGGGGAGTTGAGAACCCAAGGGGGAAGCAGGAACCAATGCCGGAAGTCTTCTTTACTGGATTTGGTGTCTGTACTTTGGATACTGAGCTGCTCCTGTCGGTGCGTGAACTGCGGCACCGTTGTCGTACTGTCGATGATCTGAACCGTACTATCGATCAGCTGGGCCGTGAAAACGACATCAACATTGCCTTTAACCAGTTTGAAGTTCATCTGCACAACgat  <  1:1074821/234‑2 (MQ=255)
                                                                                                                                                                                                                                    gTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGACCTGAACCGAACCATCGACCAGCTGTGCCGGCAAAACTACTTAAAAATTGCCTTTAACCACCTTAAAGGACATCTCACCAACgaa  >  1:1195146/1‑238 (MQ=255)
                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                        
ACGTCCGGTGCGTATTGGCGATACGGTAACCATTGGTAGCTTCTCGGGGACGGTAAGTAA‑GATCCGTATTCGTGCGACAACGATTACCGATTTCGATCGCAAAGAAGTGATCATCCCGAACAAAGCGTTTGTTACCGAGCGTCTGATCAACTGGTCGTTGACTGACACTACTACGCGTCTGGTGATCCGTCTCGGCGTGGCCTATGGCTCCGATCTGGAAAAAGTGCGTAAAGTGTTACTGAAGGCGGCGACTGAGCACCCAAGGGTGATGCACGAACCAATGCCGGAAGTCTTCTTTACGGCATTTGGTGCCAGCACGTTGGATCATGAGCTGCGTCTGTATGTGCGTGAACTGCGTGACCGTAGTCGTACTGTCGATGAGCTGAACCGTACTATCGATCAGCTGTGCCGTGAAAACGACATCAACATTGCCTTTAACCAGCTTGAAGTGCATCTGCACAACGAG  >  NC_000913/489364‑489829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A