Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F9 I0 R1 260 102.5 2178490 93.2% 2030352 226.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,257 C→T 14.0% Q123Q (CAG→CAA)  spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,2570CT14.0% 250.7 / 4.2 96Q123Q (CAG→CAA) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base C (34/47);  new base T (10/5);  total (44/52)
Fisher's exact test for biased strand distribution p-value = 9.54e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGATCAGGCTTTTTTTACAACTGT‑TTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGA  >  NC_000913/1825022‑1825491
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
tgtacacgggtttttttAACACTGTGTTGAAAAGAAAAAAAAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCt                                                                                                                                                                                                                                            <  1:721249/228‑1 (MQ=255)
          tttttttAAAATTTT‑TTGAGAAGAAAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCAtgt                                                                                                                                                                                                                                <  1:416917/239‑1 (MQ=255)
             ttttACAACTGT‑TTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTTCAGGCATTTTACCTTTTGCCGCTGGACGGTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCCTCCGGATGTTG‑TAAATTTTGTTCTTGGGTTCCTtgctgt                                                                                                                                                                                                                             >  1:426747/1‑235 (MQ=255)
                tcacactttttaggaaagataaACAAAAC‑CCGCCGATCCTTTCCACTGTATTACTGCAAGGTAGTGGACAAGGCCGGCTGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACTCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑cc                                                                                                                                                                                                                          <  1:11005/224‑1 (MQ=255)
                       tttgtgaaaaaaaaaaagaacc‑CCGCCGCTCCTTTCCACCGCATTACTGCAAGGTAGTGGACCAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGt                                                                                                                                                                                                                   <  2:823733/226‑1 (MQ=255)
                       tt‑ttGAGAAAAAAAAAAAAAACCCGCCGATCCTGTCCAACGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGt                                                                                                                                                                                                                   <  2:269773/238‑1 (MQ=255)
                        t‑ttGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTCAATTTTTTTGCTTAAAGATTATTTAGCAGTTGCAGGCCTTTTACCCTTTGCCCCTGGACGTTTTTTCAGACGCTTCTCCAAATTTACCTTTAAATGTTTTTTCTT‑TTCCGGCGTCGCGGTGTTGTTAAATTTTTTTTGGGGTTTCCTTTTGcc‑ccccctgtta                                                                                                                                                                                                                  >  2:45542/1‑231 (MQ=255)
                        t‑ttGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGCCGTTCTGTCAGACGCCTCTCAAAATTAGCATTAAATTGCTTTTTTTG‑TTCCGTGGTCCGGATGTTG‑TAAAATTTGTTCTGGGTTTCCCTTTGGG‑CCCCCATTTTa                                                                                                                                                                                                                 >  1:566519/1‑239 (MQ=255)
                        t‑ttGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTcc                                                                                                                                                                                                                                    >  1:714142/1‑221 (MQ=255)
                                   gaaaagaaaa‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTTTCAAAATTAGCATTTAATTGCTTTTTCTG‑TTCCGGCGTTAGGATGTTG‑TTAATTTTGTTCTTCGGTTCCATGTGTG‑CCACCCTGTTAACttttcc                                                                                                                                                                                                         >  1:87569/1‑234 (MQ=255)
                                   aaaaaaaaaa‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTgcg                                                                                                                                                                                                         <  1:98575/236‑1 (MQ=255)
                                    aaaagaaaa‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAACTTTTTGGCTGAAAGACTTTTTAGAAGTTGCAGGGAATTTATCTTTTTCGCCCGGACGTTCGTTCATGCGCGTCTCCAAATTAGCATTTAATTGCTTTTTCTT‑TTCCGGCTGCAGGCTGGTT‑TAAAGTTTTTTTTTGGTTTTCATGTGCT‑CACGCATGTTAACCTTtcgcgt                                                                                                                                                                                                      >  2:652297/1‑237 (MQ=255)
                                            a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCCGAAAAATTATTCAGCAAGTTCAGGCCTTTTACCTTTTGACGCTTGACGTTCTGTCAGACGCTTCTCAAAAATAACATTTAATTTCCTTTTCTT‑TTTCCGCGGCATGCTGTTT‑GAAATTTTTGTTTTGGTTTTCATTTGTG‑CCGGCATTTTAGAATTTCACTGGTTTTCCa                                                                                                                                                                                              >  2:734818/1‑239 (MQ=255)
                                            a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCAGATGCAGGCATTTTTACTTTTTGCGCTTGACGTTCTGTCCGACGCTTCTCCAACTTAGCCTTTAATTGCTTTTTTTT‑TTCCGGACGCAGGGTGGTG‑TAAATTTTTTTTTGGGGTTTCCTTTGCGCCCAGCATG‑TAGGTTTgcgtgttttttc                                                                                                                                                                                                >  2:643157/1‑228 (MQ=255)
                                             tccgccgATCCGGTCCACCGCATTACTGCAAGGTAGTGTACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCa                                                                                                                                                                                              <  1:47364/238‑1 (MQ=255)
                                             cccgccgATTCTTTCCACCGCTTTACTGAAAGGTAGTGGACAAGGCCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCa                                                                                                                                                                                              <  2:329598/238‑1 (MQ=255)
                                                  cggtCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCAttttt                                                                                                                                                                                         <  1:643157/236‑1 (MQ=255)
                                                              acGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCt                                                                                                                                                                             <  1:856775/238‑1 (MQ=255)
                                                               cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTACGCAGGTGCAGGGATTTTACCTTTTTCCGCTGGGCGTTTTTGCAGACGCTTCTCCAAATTAGCCTTAAATTGCTTTTTTTT‑TTCTGGCGTCAGGGGGTTG‑TTAATTTTTTTTTGGGTTTCCCTTTTGC‑CCCGCATTGTTGCTTTGCGTTGTTTTTTCtttttttttatcttgcgtt                                                                                                                                                                            >  2:38593/1‑226 (MQ=255)
                                                               cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGGTTTTTTGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTTCCTTTTTTCGCCGGACGTTCTGTCCGACCCCTCTTCAAATTATCATTACATTGCCTTTTCTG‑TTCCGGCGTCCGGATGTTG‑TAAATTTTTTTTTCGGGTTCCCTTTGCG‑CCACACTGGTATCTTTTTGCTGTTTTTCCCttttttgggtttcccct                                                                                                                                                                             >  2:90894/1‑226 (MQ=255)
                                                               cGCAGTAATGCAAGGTTGTGGTCAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                                                            <  1:1059373/239‑1 (MQ=255)
                                                                gCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTGTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                                                                                                                           <  1:605695/239‑1 (MQ=255)
                                                                         cAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTCAGACGCTTCTCCAAATTAGCATTAAATTTCTTTTTCTG‑TTTCCGCGTCCGGATGTTT‑GTAATTTTTTTTTTGGTTTCCCTTTGCG‑CCAGCACGTTAACTTTTGGCTGTTTTTTCCTTTTTGCGATCTGCGCCTCCGCtttt                                                                                                                                                                    >  2:93382/1‑237 (MQ=255)
                                                                         cAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTGCCGCCGGACGTTCTTTCCGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTT‑TAAATTTTTTTCTGGGTTTCCCTTTTGG‑CCCGCATGTTAGCTTTTTGCTGTTCTTCCCTTTTTTCGTATTGCGGTTTACCtttt                                                                                                                                                                    >  1:657361/1‑237 (MQ=255)
                                                                          aaGGTAGTGGAAAAGACCGGCGGTTTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                                                                                                                 <  2:954647/239‑1 (MQ=255)
                                                                           aGGTAGGGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                                                                                                                 <  1:93382/238‑1 (MQ=255)
                                                                                 tGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTTCCGCGGTCGGATGTTG‑TAAATTTTGTTTTGGGTTTCCCTGTTCG‑CCCGCAAGTTAGCTTTTGGCCGTTTTTCCCTTTTTTTGTTCTGCGCTTAAGGTTTTACTTTATTGa                                                                                                                                                          >  2:535204/1‑239 (MQ=255)
                                                                                       aggccGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                                                                                                                    <  1:756436/236‑1 (MQ=255)
                                                                                        gACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTTCTTTTTCTG‑TTCCGGCGTCCGGAGTTTG‑TAAATTTTGTTTTGGGTTTCCATTTTCG‑CCAGCATGTTAGCTTTGGGCTGTTCTTCCATTTTTGCGATCTGCGCTTTAGCTTTTACTTTTTTGAAGGTTTc                                                                                                                                                   >  1:1010155/1‑239 (MQ=255)
                                                                                        gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGAAGTTCTGTCAGAAGCCTCTCAAAATTAGCATTTAATTGCTTTTTCTG‑TTCCGGCGTCAGGAAGTTG‑TAAATTTTTTTTTGGGTTTCCATATGCG‑CCAGCATGTTAGCTTTTCGCTGTTCTTTCATTTTTTCGCTCTGCGCTTCTGCTTTTACTTTATCGAGGGTAt                                                                                                                                                    >  1:141803/1‑238 (MQ=255)
                                                                                          ccGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCg                                                                                                                                                  <  1:929836/238‑1 (MQ=255)
                                                                                           cggggtttttAAGTTTTTTGGCGGAAAGATTTTTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                                                <  2:1010155/231‑1 (MQ=255)
                                                                                           cgggggTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                                                <  1:532009/239‑1 (MQ=255)
                                                                                           cggcggTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCATGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                                                <  1:886824/239‑1 (MQ=255)
                                                                                             gcggTGTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTTCCGGGGTTTTACCTTTTGCCGCTGGACGTTCTGTCCAAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTTCCGCGTCAGGGTGTTT‑TAAAATTTGTTCTGGGTTTCCATGTGCG‑CCCGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGTGTTCTGCGCTTCAGCTTTTTTTTTATCCAAAGCATCTCTgg                                                                                                                                              >  2:756436/1‑239 (MQ=255)
                                                                                             gcggTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATTTTCG‑CCAGCATGTTAGCTTTTTGCTGTTCTTCCCTTTTTTCGAACTGCGCTTCAGCTTTTACTTTATCGAAGGTATCCCTg                                                                                                                                               >  1:582255/1‑238 (MQ=255)
                                                                                                 tCTTAAGTTTTTTGGCTGAAAGATTTTTCAGCAGTTGCAGGCAGTTTACCTTTTGCCGCTGGACGTTCTGTCAGGCGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                           <  2:643494/238‑1 (MQ=255)
                                                                                                 tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                           <  1:1070909/238‑1 (MQ=255)
                                                                                                 tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑GTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                           <  1:90894/238‑1 (MQ=255)
                                                                                                 tCTTAAGTTTTTGGGCTGAAAGATTAATCAGCAGTTGAATGCAATTTACCTTTTGGCGGTGTACGTTCTGTTCGGCGCTTCTCAAAATTAGCATTAAATTGCGTTTGCTG‑TTCCGGCCTCAGGATTTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTTCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                           <  1:491784/238‑1 (MQ=255)
                                                                                                  tttAAGTTTTTTGGCTGAAAGTTTATTCCGCCGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:880525/238‑1 (MQ=255)
                                                                                                  cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  1:547955/239‑1 (MQ=255)
                                                                                                   ttAAGTTTTTTGGCTTAAAGATTATTCAGCAGTTTCAGGCATTTTACCTTTTGCCGCTGGACGTTCTTTCAGACGCTTCTCCAAATTAACATTAAAATTCTTTTTTTT‑TTTCCGCGTCCGGGTGTTT‑TTAATTTTTTTCTGGGGTTCCATGTTGG‑CCCGCCTGTTAGCTTTTGCCTGTTTTTCCCTTTTTTTGTTTTGCCCTTCAGCCTTTTTTTTTTTGAAGGGATCGCGGGGAatga                                                                                                                                        >  2:929836/1‑239 (MQ=255)
                                                                                                   ttAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCCGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGATCTGCGCTTCAGCTTTTACTTTTTCGAAGGGATCGCTGGCAatga                                                                                                                                        >  1:374398/1‑239 (MQ=255)
                                                                                                           tttGGCTGAAAGATTATTTAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGTTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGATCTGCCCTTCAGCTTTTACTTTATCTAAGGTATCGCTGGCAATGATGTCATGc                                                                                                                                >  2:458716/1‑239 (MQ=255)
                                                                                                           tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGACGGAATCGCTGGCAATGATGTCATGc                                                                                                                                >  1:82797/1‑239 (MQ=255)
                                                                                                           tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑CACATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAaagatggaaggc                                                                                                                                >  2:308994/1‑233 (MQ=255)
                                                                                                                  taacggttattCAGCAGTTGCAGGCATTTTACCTTTTGACGCGGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcg                                                                                                                          <  1:589932/232‑1 (MQ=255)
                                                                                                                  gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcg                                                                                                                          <  1:314776/238‑1 (MQ=255)
                                                                                                                     agttttttCAGCGGTTTCAGGCATTTTACCTTTTGCCGCTGGACTTTCTGTCAGACGCTTTTTAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCgcggc                                                                                                                      <  2:648484/233‑1 (MQ=255)
                                                                                                                             cagcagTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCGGAATGTTAGCTTTGCGCTGGTCTTCCCTTTTTGCGTTCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGTTGGCATGCATTGCTGGGCGTTCTTcc                                                                                                              >  2:569600/1‑239 (MQ=255)
                                                                                                                                cagTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGTTCTGCGCTTCGGCTTTTCCTTTATCGACGGAATCGCTGCCAATGTTTTCTTGCATTGCGCGGCTTTTTTCCCgc                                                                                                           >  2:647417/1‑239 (MQ=255)
                                                                                                                                     gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcgccg                                                                                                       >  1:994300/1‑238 (MQ=255)
                                                                                                                                     gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGcggcgg                                                                                                      >  1:977629/1‑239 (MQ=255)
                                                                                                                                             tttACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                                                              >  1:767879/1‑239 (MQ=255)
                                                                                                                                              tttccTTTTGCCGCTGGACGTTCTGTCCGCCGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTCCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAt                                                                                             <  1:1006361/236‑1 (MQ=255)
                                                                                                                                              ttACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCAt                                                                                             <  1:38593/239‑1 (MQ=255)
                                                                                                                                                     ttGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGt                                                                                                                                                                                                                   <  2:752789/114‑1 (MQ=255)
                                                                                                                                                     ttGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGt                                                                                                                                                                                                                   >  1:752789/1‑114 (MQ=255)
                                                                                                                                                     ttGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCGGCGGCGGCCGTTTAATCCGGTCa                                                                                      >  2:288855/1‑239 (MQ=255)
                                                                                                                                                                   tCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCa                                                                         <  1:338601/238‑1 (MQ=255)
                                                                                                                                                                    cTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCGGTTTCATCTGGTCACCCCTGCCTTTcctt                                                                       >  2:988840/1‑236 (MQ=255)
                                                                                                                                                                     tGTCAGACGCTTCTAAAAATTTGCATTAAATTGCTTTTTCTG‑TTCCGGCTTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTgg                                                                                <  2:811054/229‑1 (MQ=255)
                                                                                                                                                                          gACGCGTCTCAAAATTAGCATTAAATTGCTTTTGCTG‑GTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                 <  1:652297/239‑1 (MQ=255)
                                                                                                                                                                            cGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                 <  1:569600/237‑1 (MQ=255)
                                                                                                                                                                               ttCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCTGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTTCG‑CCATCCTGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCACCTTTTACTTTATTGTAACTATCACTGTCAATGATTTCATGCATTCAGCGGCGTTATTCCACAGGGCGCCGTTTCATCTTGTCACCCTGGCCATTCATGTTTTctaggg                                                             >  2:374040/1‑233 (MQ=255)
                                                                                                                                                                               ttCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGa                                                                      >  1:811054/1‑229 (MQ=255)
                                                                                                                                                                               ttCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGAt                                                            <  1:535204/239‑1 (MQ=255)
                                                                                                                                                                               ttCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGCCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTAATCTGGTCACCCTTGCCTTTCTTGAttttcggggt                                                            >  2:504198/1‑232 (MQ=255)
                                                                                                                                                                                  tcAAAATTAGCTTTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTTTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATctg                                                         <  2:165254/239‑1 (MQ=255)
                                                                                                                                                                                      aaTTAGCATTAAATTTCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATTTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGt                                                     <  1:732565/239‑1 (MQ=255)
                                                                                                                                                                                        ttttcttttaaTTGTTTTTTTTG‑TTCCGGCGTTAGGATTTTG‑TAAATTTTGTTTTGGGTTTCCATGGGCG‑CCAGCATGTTAGCTTTGCGCTTTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGttt                                                   <  2:759312/230‑1 (MQ=255)
                                                                                                                                                                                         tAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTACTGGGTTTCCATGTGCG‑ACAGCATGTTAGGTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGATGGCCTTTCATGATTTCGCGGATCTTCTGatta                                                  >  1:916806/1‑238 (MQ=255)
                                                                                                                                                                                                 tattttttttttTGTTTCCGGCTGTAGGTTTTTT‑TAAATTTTTTTCTGGGTTTCCCTGGGCG‑CCAGCATGTTAGTTTCGCCCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTCGCGCTGGCACTGATTTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCACCTGGTCTCGCTTGACTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGt                                           <  2:394645/233‑1 (MQ=255)
                                                                                                                                                                                                    tgtttttttTG‑TTCCGGGGTTAGGTTGTTG‑TAATTTTTGTTCGGGTTTTCCAGGGGCG‑GCAGCAGGTGTGCTTTGCGCTGGTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCAGGCATTGCGCGGCGTGCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTTGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGt                                       <  2:657361/236‑1 (MQ=255)
                                                                                                                                                                                                     gCTTTTTCCG‑TTCCGGCGTCAGGATTTTT‑TAAATTTTGTTCTGGGTTTCCATTTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc                                      <  1:308994/239‑1 (MQ=255)
                                                                                                                                                                                                      cTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTTCGCGTCGGtca                                     >  1:81631/1‑239 (MQ=255)
                                                                                                                                                                                                         tttCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTTTGCGCGTCGGtcaggt                                  >  1:1041648/1‑239 (MQ=255)
                                                                                                                                                                                                         tttCTG‑GTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggt                                  <  1:720165/239‑1 (MQ=255)
                                                                                                                                                                                                                ttCCGGCGTCAGGTTGTTT‑TTAATTTTGTTTGGGGTTTCCATGTGCG‑CCAGCCTTTTAGCTTTGCGCTTTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttcaggt                            <  2:544211/239‑1 (MQ=255)
                                                                                                                                                                                                                ttCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGTTTTCGCGGATCTGCTGTTTTTGCGCGTCGGtcaggttcag                              >  1:544211/1‑237 (MQ=255)
                                                                                                                                                                                                                 tCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCCTTTGGTGACGCTGGCCTTTCCTGTTTTCGCGGAGCTGCTGTTTTTGCCGGTCGGgcaggttccgg                             >  2:159508/1‑237 (MQ=255)
                                                                                                                                                                                                                 tCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGGGCGTCGGtcaggttcagg                             >  1:603037/1‑237 (MQ=255)
                                                                                                                                                                                                                 tCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttcagg                             >  1:405400/1‑237 (MQ=255)
                                                                                                                                                                                                                     gggTCAGGTTGTTT‑TAAATTTTGTTTTGGTTTTCCATTTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTg                       <  2:870261/237‑1 (MQ=255)
                                                                                                                                                                                                                     gCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCACCTGGTCACGCTGGCCTTTCATGCTTTCGCGGATCTGCTGTTTCTGCGCGTCGGCCAGGTTCAGGTCTTTg                       >  1:411408/1‑239 (MQ=255)
                                                                                                                                                                                                                      ggTCAGGTTTTTT‑TAAATTTTGTTTTGGGTTTCCATGTGCG‑CCAGCAGGTTAGCTTTGGGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGa                      <  2:582255/238‑1 (MQ=255)
                                                                                                                                                                                                                        gttaggttttttttaaTTTTGTTTTGGGTTTCCATGTGCG‑CCAGCATGTTTGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGa                      <  2:1041648/224‑1 (MQ=255)
                                                                                                                                                                                                                           ggATGTTG‑TAAATTTTGGTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTGTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  1:207338/239‑1 (MQ=255)
                                                                                                                                                                                                                           ggAGGTTG‑TAAATTTTGTTCTGGGTTTCCAGGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  1:654395/239‑1 (MQ=255)
                                                                                                                                                                                                                            gggttttttattttttttttTGGGTTTCCCGTTCCG‑CCAGCCTTTTAGCTTTGCGCTGTTCTTCCATTTTTGGGATTTTCGCTTCAGCTTTTACTTTAACGAAGGTATCGCTTGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTTCCGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  2:405400/227‑1 (MQ=255)
                                                                                                                                                                                                                            gATGTTT‑GAAATTTTTTTCTGGTTTTCCTTGTGCG‑CCAGCAGTTTAGTTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  2:603037/238‑1 (MQ=255)
                                                                                                                                                                                                                            gATGTTG‑TAAATTTTGGTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGGTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTTCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  1:326663/238‑1 (MQ=255)
                                                                                                                                                                                                                                ttg‑tAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGGTTCTGCCAGTCGGTCAGGTTCAGGTCTTTGACCATCATGt             >  1:65196/1‑238 (MQ=255)
                                                                                                                                                                                                                                       aTTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTTGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGGCGGTCAGGTTCAGGTCTTTTAACCTCATTTCCTGGTg      >  2:1028033/1‑239 (MQ=255)
                                                                                                                                                                                                                                           tGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCCCGGGTCTGCTGTTTCTGCGCGTCGGTCCGGTTCAGGTCTTTTAACACCATGTCCTGATGCgg   >  2:1076925/1‑238 (MQ=255)
                                                                                                                                                                                                                                           tGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTGCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGa  <  1:647417/239‑1 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                           
GTGATCAGGCTTTTTTTACAACTGT‑TTGAGAAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTCAGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTG‑TTCCGGCGTCAGGATGTTG‑TAAATTTTGTTCTGGGTTTCCATGTGCG‑CCAGCATGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGA  >  NC_000913/1825022‑1825491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A