Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F27 I0 R1 322 127.1 2757226 91.8% 2531133 223.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,257 C→T 10.7% Q123Q (CAG→CAA)  spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,2570CT10.7% 377.8 / 3.7 137Q123Q (CAG→CAA) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base C (50/70);  new base T (14/3);  total (64/73)
Fisher's exact test for biased strand distribution p-value = 3.19e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 138 total aligned reads displayed.

ATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGA  >  NC_000913/1825025‑1825491
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTTCGCAGTTGCAGGCATTTTTCCTTTTTCCGCTGGGCGTTCTTT‑CAG‑ACCCTTCTCAAAATTAGC‑ATTTAATTTGTTTTTTTTT‑TTCTGCGTCCGGGTTTTTTAAATTTTTTTT‑T‑GGGtt                                                                                                                                                                                                                                       >  2:1122507/1‑239 (MQ=255)
aTCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGGATATTCAGCAATTTTAGGCATTTTTTCCTTTGCCGCTGGTCGTTTCTT‑TAG‑CACCTTCTCAAAATTAGC‑TATAAATTTCTTTTTCTGT‑TTCTGCGTCCGGCAGTTTTAAAATTTGTTT‑T‑GGGtt                                                                                                                                                                                                                                       >  2:50946/1‑239 (MQ=255)
    ggCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAATTTTTTGGCTGAAAAATTATTTAGCAGGTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTTT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTTAATTGCTTTTTCTGT‑TTCGGCGTCCGGATGTTGTAAATTTTGTTC‑T‑GGGtttt                                                                                                                                                                                                                                     >  1:1228476/1‑236 (MQ=255)
      ttttttttACAATTGTTTGAAAAAAAAAAAAAAACA‑CCGCCCATCCTGTCCACCTCATTACTGGAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCAt                                                                                                                                                                                                                                  <  2:816048/238‑1 (MQ=255)
       tttttttACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCATCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAAGTTTTTGCCTGGAAGATTATTCATCGCTTGGAGGCATTTTATCTTTTTCCGCAGGCGGTTTTTT‑TAG‑ACGATTGCCCAAATTATC‑ATTAAATTGCTTTTTCTGT‑TCCTGCGCCAGGAGGTTTTACATTTTGTGT‑T‑GGGGTTcctgtt                                                                                                                                                                                                                                >  2:741709/1‑235 (MQ=255)
          ttttACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGGCGGTCTGT‑CAG‑ACGCCTCTCAAAATTAGC‑ATTAAATTGCTTTTTTTGT‑TCCGGCGTCAGGGTGTTGTAAATTTTGTTC‑T‑GGGGTTCCATTTgc                                                                                                                                                                                                                              >  1:138886/1‑238 (MQ=255)
                   ttttagaaaaagaaaaaaaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGt                                                                                                                                                                                                                   <  1:1065352/226‑1 (MQ=255)
                   ttgaaaaaaaaaaaaaaaaaa‑c‑CCGCCGATCCTGTCCCCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGt                                                                                                                                                                                                                   <  2:159469/226‑1 (MQ=255)
                     tttGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTTAGTTTTTTTGCTGAAAGATTATTCAGCAGGTGGAGGGATTTTTACTTTTTCCGCTGGACGGTTTTT‑CAG‑AAGCTTCTCAAAATTAACAATAAAATTTCTTTTT‑TTT‑TTTGGCGGCAGGATGTTGTAAAATTTTTTT‑T‑GGGGTTTCAtttggggccccatggta                                                                                                                                                                                                                  >  2:1006902/1‑226 (MQ=255)
                       tGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGGTGAAAGATTATTCAGCAGTTGCAGGCATTTTATCTTTTTCCGCCGGACGTTCTGT‑CAT‑GAGCGTCTCAAAATTTGC‑ACTAAAATTCTTTTTTTTT‑TTCGGGGGCCGGAGTTTGTAAAATTTGTTC‑T‑GGGttttccttttggcccccaatttg                                                                                                                                                                                                                  >  2:146550/1‑217 (MQ=255)
                            agaaaaAAGAAA‑T‑CCGGCGGTCCTGTCCACCGGATTTCGGCAAGGTGGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgc                                                                                                                                                                                                          <  2:32856/239‑1 (MQ=255)
                             aagaaaagaaa‑C‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                                                                                                                                                         <  1:330609/238‑1 (MQ=255)
                             aaaaaaaaaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTGTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                                                                                                                                                         <  1:1174510/236‑1 (MQ=255)
                              agaaaaggac‑ACCCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGGTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTgcg                                                                                                                                                                                                         <  1:1306048/239‑1 (MQ=255)
                               gaaaagaaa‑a‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGTCGTTCTGT‑CAG‑ACGCTTCTCCAAATTAGC‑ATTAAATTGCTTTTTTTTT‑TCCGGCGGCCGGGTGTTTTAAATTTTTTTCTT‑GGGTTTCATTGGGC‑CCAGCA‑CGTTaagttttgggt                                                                                                                                                                                                       >  2:760743/1‑229 (MQ=255)
                                                   ccTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCg                                                                                                                                                                                      <  1:278358/238‑1 (MQ=255)
                                                    cTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGAt                                                                                                                                                                                    <  1:1095947/239‑1 (MQ=255)
                                                      gTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTCAGCAGGTGCAGGCCTTTTACCTTTTTCCGCTGGACGGTCTGT‑CAA‑ACGCTTCTCAAAAATAAC‑CATTAATTTCTTTTTTTTT‑TCCCGCGTCCTGTGGTTTTAAAATTTGTTT‑T‑GGGGTTTCCTGTTCCCCAGCA‑AGGTAGCTTTTCCGTTTTTTTCCCtttttttggttt                                                                                                                                                                                  >  2:921162/1‑232 (MQ=255)
                                                       tcccacGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                                                                 <  1:1336437/234‑1 (MQ=255)
                                                            ccagttACTGCAAGGTAGGGGACAAGACTGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGATTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                                                            <  1:908689/235‑1 (MQ=255)
                                                            cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTTGCTGGAAGATTATTTAGCAGTTGCAGGCCTTTTTCCCTTTTCCCCCGGACGTTCTTT‑CCG‑ACGCTTTTCAAAATTAGA‑CTTTAATTGCTTTTTTTGT‑TCCCGCGTCCGGATGTTTTAAATTTTGTTT‑T‑TGGTTTTCCTTTTCGCCAGCC‑TTTTGACTTTTGCCTGTTTTTCCATTTTTTCGATTTGCGCt                                                                                                                                                                             >  2:582178/1‑238 (MQ=255)
                                                            cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATTTGCGCCAGCA‑TGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCtcggct                                                                                                                                                                             >  1:1114478/1‑233 (MQ=255)
                                                            cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTTTTGTTTTTTGGGTGAAAGGTTATTCAGCAGATGGAGGCATTTTTCCTTTTTCCGCTGGCCGGTCTGT‑CAG‑ACACTTCTCAAAATTAGC‑ATTAAATTGCTTTTTTTGT‑TTCGGCGTCAGGGTGTTGTAAATTTTTTTT‑T‑GGGTTTTCATTGTCGCCCGCA‑TGTTAACTTTGAGCTGTTTTTCCATTTTTTTGATCTgcggt                                                                                                                                                                             >  2:932770/1‑236 (MQ=255)
                                                             gCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                                                                                                                           <  1:574006/239‑1 (MQ=255)
                                                                 tACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCCTTTTACCTTTTTCCGCTGGACGTTCTGT‑TAA‑ACGCTTCTCCAAATTAGC‑ATTTAATTGCTTTTTTTGT‑TCCGGCGTCAGGATGTTGTAAATTTTTTTT‑T‑GGGTTTTCTTTTGGGCCCGCA‑TGTTAGCTTTGCGCTGTTTTTCCATTTTTTCGATTTGCGCTTCCGCt                                                                                                                                                                       >  2:471777/1‑239 (MQ=255)
                                                                   cTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGGTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTTCATTTTTTCGGTCTGCGCTTCAGCttt                                                                                                                                                                     >  1:753614/1‑239 (MQ=255)
                                                                      cAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGAAGTTGCAGGCATTTTAACTTTTGCCGCTGGACGTTTTTT‑CAG‑AAGCTTCTCCAAATTAGC‑ATTAAATTTCTTTTTCTTT‑TCCGGCGTCAGGAGGTTGTAAATTTTGTTC‑T‑GGGTTTCCCTGTGCGCC‑GCACTGTTAGCTTTGCGCTGTTCTTCCCTTTTTTCGATCTGCGCTTCCGCtttt                                                                                                                                                                    >  1:1155205/1‑237 (MQ=255)
                                                                        aGGTAGTGGACTAGACCGGCTGTCTTAAGTTTTTTGGCTGAAAAATTATTCAGCAGTTGCAGGCATTTTACCTTTGGGCGGTGGACGTTCTGT‑CAG‑GCGCTTCTCAAAATTAGC‑ATGAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTTTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                                                                                                                 <  1:1089417/238‑1 (MQ=255)
                                                                         ggTAGTGGACGAGACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGTCGCCGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTTAATTGCTTTTTTTGT‑TCCGGCGTCCGGATGTTGTAAATTTTGTTC‑T‑TGGGTTCCATGTGCGCCAGCC‑AGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGATTTTCGCTTTAGCTTTTACttt                                                                                                                                                               >  1:197230/1‑239 (MQ=255)
                                                                           tAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCAttttt                                                                                                                                                                                         <  1:850627/211‑1 (MQ=255)
                                                                            tgTGGACAAGACCGGCGGTCTTAAGTTTTGTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCGGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTAt                                                                                                                                                             <  1:435126/237‑1 (MQ=255)
                                                                              tGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTTTCAAAATTAGC‑ATTAAATTGCCTTTTTTGT‑TCCGGCGTCAGGATGTTGTAAACTTTGTTC‑T‑TGGTTTCCATGTCGGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCCTTTTTTCGATTCTCCCTTCCCCCTTTACTTTTTcta                                                                                                                                                          >  1:1043413/1‑237 (MQ=255)
                                                                              tGGACAAGACCGGCGGTCTTAAGGTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGCTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTTTGT‑TCCGGCGTCAGGATGTTGTAAATTTTTTTC‑T‑GGGGTTCCATGTGCGCCAGCA‑TGTTAGCTTTTCGCTGTTCTTCCATTTTTTCGGTCTGCGCTTCCGCTTTTACTTTATCGa                                                                                                                                                          >  1:744947/1‑239 (MQ=255)
                                                                                gACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGcc                                                                                                                                                                                                                           <  1:451964/173‑1 (MQ=255)
                                                                                   aaGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTT‑T‑GGGTTTTCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTTCGATCTGCGCTTCAGCTTTTACTTTTTCGAAGGt                                                                                                                                                      >  1:309303/1‑238 (MQ=255)
                                                                                     gACCGGCGGTCTTAAGTTTTTTTGCTGAAAGATTATTTAGCCGGTGCACGCATTTTACCTTTTTCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTTAATTTATTTTTTTTT‑TTCTGCGTCAGGAGGTTGTAAATTTTGTTT‑T‑TGGTTTCCCTTTTTGCCAGCC‑TGTTAGCTTTTGGGTGTTTTTCCTTTTTTTCGATCTGCCCTTCCGCTTTTTTTTTTTCGTAggatt                                                                                                                                                    >  2:347021/1‑235 (MQ=255)
                                                                                     gACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCCTGTGCGCCATAC‑TGTTACCTTTTGGCTGTTTTTCCATTTTTGCGATTTGCGCTTCAGCTTTTTCTTTATTGAAGGAAt                                                                                                                                                    >  1:162436/1‑238 (MQ=255)
                                                                                     gACCGGCGGTCTTAAATTTTTTTGCTGAAAGATTATTTAGCAGTTGCAGGGATTTTTCCTTTTGCCCCTGGACGTTTTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGTTTTTTTTGT‑TTCGGCGGCTGGAGGTTGTTAATTTTGTTT‑T‑GGGTTTCCTTTTTCGCCAGCA‑TGTTAGCTTTGTGCTGTTTTTCCCTTTTTTCGGTTTGCGCTTCACCTTTTCCttttttgtagggttc                                                                                                                                                   >  2:302938/1‑227 (MQ=255)
                                                                                        cggccgTCTTAAGTTTTTTTGCTGAAAGATTATTCAGGAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCGCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                                                <  1:302938/239‑1 (MQ=255)
                                                                                              tCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                           <  1:136085/238‑1 (MQ=255)
                                                                                               ttttaGTTTTTTGGCTTAAAGATTATTCCGGATTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:197230/235‑1 (MQ=255)
                                                                                               tttagtgtttttGTCTTAAAGATTTTTCAGCAGGTGGAGGCGTTTTTCCGTTTGCCTGTGGAC‑TTTTGTGCAG‑ACGCTTCTTAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGGCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:1275387/232‑1 (MQ=255)
                                                                                               ctttatttttttGGTGTAAAGTTTATTCAGCAGTTGTAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCTGCAGGATGTTGTAAATTTTGTTC‑G‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:1378050/233‑1 (MQ=255)
                                                                                               cTTAAGTTTTTTGGCTGAAAGGTTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  1:72366/239‑1 (MQ=255)
                                                                                               cTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  1:146550/239‑1 (MQ=255)
                                                                                                    gTTTTTTTGTTTAAAGATTATTAAGCGGTTGCAGGCATTTTACCTTTTGCCGCTGGCCGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTTT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTc                                                                                                                                    <  2:1124079/239‑1 (MQ=255)
                                                                                                      tttttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTc                                                                                                                                    <  1:61071/237‑1 (MQ=255)
                                                                                                        tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGCTGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGGCagga                                                                                                                                >  2:535468/1‑236 (MQ=255)
                                                                                                        tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTTTTCCATTTTTGCGGTCTGCGGTTCAGCTTTTACTTTATTGAAGGTATCGTTGCCAaagattcatagc                                                                                                                                >  2:739890/1‑232 (MQ=255)
                                                                                                        tttGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTGGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAGGGGATCCCTGGCAATGATGTccg                                                                                                                                  >  1:1378050/1‑235 (MQ=255)
                                                                                                               gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         >  1:317871/1‑223 (MQ=255)
                                                                                                               gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTAATGCATTgtgc                                                                                                                         >  1:220052/1‑239 (MQ=255)
                                                                                                               gAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTGCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:317871/223‑1 (MQ=255)
                                                                                                                      tattCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGACTTCATGCATTGCGCGGGGt                                                                                                                    >  2:1064551/1‑237 (MQ=255)
                                                                                                                       attCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTAGGCTTTTACTTTATCGAAGGCATCGCTGGCAATGAGGTCATCAATTGCGCGGCGttc                                                                                                                  >  2:605415/1‑238 (MQ=255)
                                                                                                                        ttCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  2:1210286/214‑1 (MQ=255)
                                                                                                                             cagTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCCCGGCGTCCTTCCAgc                                                                                                           >  1:483971/1‑239 (MQ=255)
                                                                                                                              agTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcg                                                                                                          >  2:311373/1‑239 (MQ=255)
                                                                                                                                ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggc                                                                                                        >  1:1321102/1‑239 (MQ=255)
                                                                                                                                 tGCAGGCATTTTACCTTTTGCCGCTTGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                                                       <  1:50946/239‑1 (MQ=255)
                                                                                                                                 tGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                                                       <  1:344191/239‑1 (MQ=255)
                                                                                                                                          tttACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                                                       <  2:1100620/230‑1 (MQ=255)
                                                                                                                                          tttACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                                                              >  1:789797/1‑239 (MQ=255)
                                                                                                                                          tttACCTTTTGCCGATGGACGTTCTGG‑CGGTACG‑TTCTCAAAATTAGC‑ATTGAATTGGTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTTCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCa                                                                                              <  1:888338/239‑1 (MQ=255)
                                                                                                                                              ccTTTTGCCGCTGTGCGTTCTGT‑CGG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGG‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTg                                                                                          <  1:989785/239‑1 (MQ=255)
                                                                                                                                                ttttGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                                                                        <  1:1114894/239‑1 (MQ=255)
                                                                                                                                                ttttGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                                                                        <  1:1006902/239‑1 (MQ=255)
                                                                                                                                                 tttGCCGCTGGACTTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑TGGGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                                                                        <  1:141695/239‑1 (MQ=255)
                                                                                                                                                    tcccttGGTCGTTCTGT‑CAG‑ACTCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACg                                                                                    <  2:1240299/234‑1 (MQ=255)
                                                                                                                                                         tGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCt                                                                                  <  1:527137/236‑1 (MQ=255)
                                                                                                                                                                tCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTGAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                         <  1:570097/174‑1 (MQ=255)
                                                                                                                                                                tCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACCCTGGCCTTTCAt                                                                        >  2:445967/1‑239 (MQ=255)
                                                                                                                                                                   gT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCCCGGCGTTCTTCCAGCGGCGGCCCTTTCTTCTGGTCACGCTGGCCTTTccggct                                                                     >  2:527137/1‑234 (MQ=255)
                                                                                                                                                                         tcctctttttaaatTTATC‑TTTAAATTGTTTTTTCTGT‑TCCGGCTGCAGGATGTTGTAAATTTTTTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTGTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                 <  2:1357860/229‑1 (MQ=255)
                                                                                                                                                                           cGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                 <  1:1066807/237‑1 (MQ=255)
                                                                                                                                                                             cTTTTCAAAATTTGC‑TTTTAATTTGTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                                                             <  2:1306381/239‑1 (MQ=255)
                                                                                                                                                                              ttCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGa                                                             >  1:631651/1‑238 (MQ=255)
                                                                                                                                                                               tctcAAATTTTGC‑ATTAAATTGTTTTTTCTGT‑TCCGGCGTCAGGATGTTGGAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATc                                                           <  2:1155205/239‑1 (MQ=255)
                                                                                                                                                                                ctcAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCCCGGa                                                             >  1:1306381/1‑236 (MQ=255)
                                                                                                                                                                                        tAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                                                            >  1:1116975/1‑117 (MQ=255)
                                                                                                                                                                                        tAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                                                       >  2:166811/1‑186 (MQ=255)
                                                                                                                                                                                          agtaTTAAATTGCTTTTTCCTG‑TCCGGGGTTAGGATTTTTTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCATCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGTAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCt                                                 <  2:789797/236‑1 (MQ=255)
                                                                                                                                                                                           c‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                                                                 >  1:1027456/1‑109 (MQ=255)
                                                                                                                                                                                           c‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTg                                                                                                                                                                                 <  2:1027456/109‑1 (MQ=255)
                                                                                                                                                                                               tAAATTGCTTTTTCCGT‑TCCGGCGTCAGGATTGTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGcgcg                                            <  1:691631/239‑1 (MQ=255)
                                                                                                                                                                                                      tttttttTGTGTCCGGGTTGAGGTTTTTGTGAATTTTTGTC‑T‑GGGTTTCGATGTGCGCCAGCA‑GGTTAGCTTTTCGGTTTTCTTCCATTTGTGCGATGATCGCTTCAGCTTTTATGTGATCGAAGGTATGGCTGGCAATGATGTCATGCAGTTCGCGGAGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc                                      <  2:587013/238‑1 (MQ=255)
                                                                                                                                                                                                                  ccGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCTATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTAACGCTGGCCTTTCATGATTTCGCGGGTCTGCTGATTCTGGGCGTCGGTCAGGTTCAGGGCt                          >  1:604442/1‑239 (MQ=255)
                                                                                                                                                                                                                       gTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGa                      <  1:1124232/238‑1 (MQ=255)
                                                                                                                                                                                                                          aGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTTAAca                   >  1:264500/1‑238 (MQ=255)
                                                                                                                                                                                                                          aGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCCCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAca                   >  1:370301/1‑238 (MQ=255)
                                                                                                                                                                                                                           ggttttttttaaTTTTTTTT‑T‑GGGTTTCCCTGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCTTTTTTGCGATCTGCGCTTTAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  2:370301/229‑1 (MQ=255)
                                                                                                                                                                                                                           ggtttttttAAATTTTTTTT‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  2:1114478/231‑1 (MQ=255)
                                                                                                                                                                                                                             aTGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  1:1226524/237‑1 (MQ=255)
                                                                                                                                                                                                                             aTGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCCATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGTCGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTTCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                 <  1:825722/237‑1 (MQ=255)
                                                                                                                                                                                                                                 tgtAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTGAGGTTCCGGTCTTTGGAcatacggtcc           >  2:1203323/1‑232 (MQ=255)
                                                                                                                                                                                                                                  gtAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGt             >  1:1294648/1‑236 (MQ=255)
                                                                                                                                                                                                                                  gtAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGCCTTTGAACATCATGt             >  1:1073930/1‑236 (MQ=255)
                                                                                                                                                                                                                                      ttttttttt‑t‑tGGTTTCCATTGTCGCCAGCA‑TTTTTGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTCCTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGATGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATg      <  2:236752/238‑1 (MQ=255)
                                                                                                                                                                                                                                          tGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTTATTCgg   >  1:835214/1‑238 (MQ=255)
                                                                                                                                                                                                                                          tGTTC‑T‑GGGTTTCCAGGTGCTGCAGCA‑TGTTAGCTTTGCGCTGGTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGa  <  1:278813/239‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                             
ATCAGGCTTTTTTTACAACTGTTTGAGAAGAGAAAAGAAA‑A‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAGTTTTTTGGCTGAAAGATTATTCAGCAGTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGT‑CAG‑ACGCTTCTCAAAATTAGC‑ATTAAATTGCTTTTTCTGT‑TCCGGCGTCAGGATGTTGTAAATTTTGTTC‑T‑GGGTTTCCATGTGCGCCAGCA‑TGTTAGCTTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGA  >  NC_000913/1825025‑1825491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A