Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F17 I0 R1 160 98.0 2214160 89.0% 1970602 222.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,825,257 C→T 15.5% Q123Q (CAG→CAA)  spy ← periplasmic ATP‑independent protein refolding chaperone, stress‑induced

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,825,2570CT15.5% 196.4 / 5.5 87Q123Q (CAG→CAA) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
Reads supporting (aligned to +/- strand):  ref base C (33/38);  new base T (12/2);  total (45/42)
Fisher's exact test for biased strand distribution p-value = 8.32e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACC  >  NC_000913/1825024‑1825493
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
gATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTTAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGGCGTTCTGTA‑AGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCCGCGT‑CAGGATGTTGGTAATTTTGTTCTTGGt                                                                                                                                                                                                                                           >  1:781501/1‑239 (MQ=255)
   cAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTTAGCA‑GTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGT‑CCGGATGTTGTAAATTTTTTTTTGGGgtt                                                                                                                                                                                                                                         >  1:961401/1‑238 (MQ=255)
   cAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGGTTTTTTGCTGGAAGATTATTCAGAA‑GTTTCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTT‑AGACACTTCTCCAAATTATCATTATATTGCGTTTTTTGTTCCGGCGTCCAGGA‑GGTGTAAAATTTTTTCTTGGgtt                                                                                                                                                                                                                                         >  2:57033/1‑238 (MQ=255)
      gCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACAGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑CAGTTTTTTTGCTTAAAGATTTCTTCGCA‑GTTGCAGGGCTTTTACCTTTTTCCCCTGGAGGGTCTTTC‑CTACGCTTCTCAAAATATCCTTTTAATATCCTTTTCTCTTCTCGCGT‑CAGGCTGTTGTAAATTTTTTTTTGGGtttgcct                                                                                                                                                                                                                                     >  2:5770/1‑235 (MQ=255)
        tttttttAGATTTG‑TTGAGAAAGAAAAAAAAAAC‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGGCCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑tgt                                                                                                                                                                                                                                  <  1:284529/239‑1 (MQ=255)
         ttttttACAACTGTTTGAG‑AAGAGAAGAGAAAA‑CCGCCGCTCCTGTACACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTT‑ATGTGTTTTTGCTGGAGAATTTTTTCGCATGTTAGGGGC‑TTTTAACTTTTTCAGATGGAACTTCTCTC‑AGACGCCTCTCCAACTTACCGTTTAGTTACCTTTTTTCTTCGCGCGT‑CAGTGTAATTTCAATTTTGTTCTGGGTTTctagtt                                                                                                                                                                                                                                   >  2:67682/1‑233 (MQ=255)
         ttttttACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGCCTT‑AAGGTTTTTTGCGGAAAAATTTTTTAGCA‑GTTTCAGGGATTTTTCCTTTTGTCACTGGACGTTCTTTC‑CGAAGGCTCTCAAAATTAGCACTTTATTTTTTTTTTTTTTTCGGTGC‑CAGGGTGTTTTAAAATTTTTTTTGGGGTTccctttt                                                                                                                                                                                                                                  >  2:265947/1‑234 (MQ=255)
           ttttACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGCCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGGAGGCCATTTACCTTTTGCCGCTGGACGTTCTGTC‑AGAGCCTTCTCAAAATTAGCAGTAAATTTCTTTTTCTGTTCCGGCGG‑CAGGATGTTGGAAATTTTTTTCTTGGGTTCCA‑TTTgc                                                                                                                                                                                                                                >  1:1039613/1‑238 (MQ=255)
            ttcaCAATTGTTGGA‑‑AAGAAAAAAAAAAACCCGCCGATCCTGTCCTCCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTgcgc                                                                                                                                                                                                                              <  2:1073144/236‑1 (MQ=255)
                     gTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACATTTTGCCGCTGGACGTTTTGTC‑AGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTCTGTTCCGGCGT‑CAGGGTGTTGTTAATTTTGTTTTTGGGTTCCA‑TTTTCGCCAGctggtt                                                                                                                                                                                                                     >  1:1105200/1‑234 (MQ=255)
                      tttGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTTGCTGGAAGATTATTTAGCC‑GTTGTAGGCCATTTACCTTTTTCCGCTGGGCGGTCTGTCAAAACGC‑TCTCCAAATTATCATTTAATTTCCTTTTTTTTTCCCGCGG‑CCAGTTCTTTACAATTTTTTTCTGGGTTTccctttgtcccacgcagtta                                                                                                                                                                                                                     >  2:597457/1‑221 (MQ=255)
                      tttGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTATCATTAAATTGCTTTTTCTGTTCCCGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGGTTCCC‑TTTGCGCCAGCATTTTa                                                                                                                                                                                                                    >  1:686041/1‑239 (MQ=255)
                      tttGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGGTTTTTTGGTGAAAGATTATTCAGCA‑GTTTCAGGGATTTTTCCTTTTTCCGCTGGACGTTCTGTC‑AAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTTTGGGTTTTCC‑TCTTCGCCCGCATGTTa                                                                                                                                                                                                                    >  1:115633/1‑239 (MQ=255)
                        tGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGGCTT‑AAATTTTTTTGCTGAAAGATTATTTAGCA‑GATTCAGGCATTTTTCCTTTTTTCCCTGGACGTTTTGTT‑CGACGCTTCTCAAAATTTACATTAAATTTCTTTTTTTTTTTCGGCGC‑CCGGGTGTTGTAAATTTTTTTTTTGGTTTctcttttgcccacactgtgtga                                                                                                                                                                                                                   >  2:345574/1‑218 (MQ=255)
                        tGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGCAGTGGACAAGACCGGCGGTCTT‑AAGGTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTTTGTC‑AGACGCTTCTCAAAATTAGCATTTAATTGCTTTTTTTGTTCCGGCGT‑CATGTTGTTGTAAATTTTTTTCTGGGGTTCCA‑TGTGCGCCAGCATGTTa                                                                                                                                                                                                                    >  1:180373/1‑237 (MQ=255)
                                 aaaaaaaaac‑CCGCCGATCCTGTCCACCGCATTACGGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                                                                                                                           <  1:591102/236‑1 (MQ=255)
                                                  aTCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTC‑AAACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTTTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTTCGCTGTTCTTTCCttttttc                                                                                                                                                                                         >  1:550758/1‑237 (MQ=255)
                                                   tCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGcc                                                                                                                                                                                                                             <  1:1022347/203‑1 (MQ=255)
                                                            ccggtttACTTAAAGGTGGGGCACAAGCCCGGCGGTTTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAATATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCt                                                                                                                                                                               <  2:550758/234‑1 (MQ=255)
                                                             cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTTAAAGTTTTTTGG‑TGAAAGAGTATTTAGCA‑ATTTTCAGCAGTTTTCCTTTTTCCTCTGGGAGTTCTTTC‑AAACGCCTCTCAAAAATAACATTAAAATCATTTTTTTTGTCCGCGGT‑CAGGAATTTTGCAATTTTGTTTTGGGTTTCCACTTTCCGCCCGC‑TTTTAGA‑TTTTCACTTTTTTTCCATTTTTTCGCTATgcgcc                                                                                                                                                                               >  2:964428/1‑237 (MQ=255)
                                                             cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTTGCTTAAAGATTTTTCAGCA‑ATTTCAGGCATTTTTCCTTTTTCCGTTGGACGTTCTTTC‑AGACGCTTCTCAAAATTAGCATTAAATTTCCTTTTCTTTTCCGGCGT‑CAGGTGGTTGTAAATTTTGTTCTGTTGTTCCA‑TGTGCCCCGCCATGTTAAC‑CTTATGCTGTTCTCCCTTTTTTGCgtacttgcct                                                                                                                                                                               >  1:557899/1‑229 (MQ=255)
                                                             cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGGTTTTTGGCTGAAAGATTATTCAGCA‑GGTGCAGGCATTTTAACTTTTTTCGCTGGACGTTTTGTC‑AGACGCTTCTTCACAATAGCATTAAATTTCTTTTTTTGTTCCGGCGT‑CAGGATGTTTTAAATTTTGTTCTGGGTTTCCC‑TGTTGGCCAGCCTGTTAGCTTTTTGGCTG‑TCTTCCCTTTTTGCGATCTGCCCt                                                                                                                                                                               >  1:502680/1‑238 (MQ=255)
                                                             cGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGCTTTTTTGCTGAAAGATTATTTAGCA‑GTTGCGGGCATTTTAACTTTTTCCCCTGGACGGTCTGTC‑AGACCCCTCTCCAAATTAGCCTTAAATTTCCTTTTCTTTTTCGGCGC‑CCGGGTGTTTTAAATTTTGTTTTTGGGTTCCA‑TTGGCGCCAACATGTTTGC‑GTTTCGCTGTTCTTCCCttttttctgttttcgct                                                                                                                                                                               >  2:587453/1‑226 (MQ=255)
                                                              gCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTc                                                                                                                                                                             <  1:837034/239‑1 (MQ=255)
                                                                     tGCAAGGTAGTGGACAAGCCCGGCGGTCTT‑AAGTTGTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAg                                                                                                                                                                                                                           <  1:782513/187‑1 (MQ=255)
                                                                       cAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGcc                                                                                                                                                                                                                             <  1:5937/183‑1 (MQ=255)
                                                                        aaGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCGGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACt                                                                                                                                                                   <  1:587453/239‑1 (MQ=255)
                                                                          ggTAGTGGACAAGACCGGCGGTCTT‑AAGGTTTTTTGCTGAAAGATTATTCAGCA‑GTTGCAGGGATTTTACCTTTTTCCGCTGGACGTTTTGTC‑AGACGCGTCTCAAAATTTGCATTAAATTGCTTTTTTTGTTTCGGCGT‑CAGGATGTTGTTAATATTTTTTTTGGTTTTCC‑TTTGCGGCAGCATGTTTGA‑TTTGTGCTGTTTTTTCATTTTTTTGATTTGCGCTTTAGCTTTTACttt                                                                                                                                                                 >  2:445767/1‑239 (MQ=255)
                                                                                ggACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCg                                                                                                                                                             <  1:386437/237‑1 (MQ=255)
                                                                                    aaGACCGGCGGTCTT‑AAGTTTTTTTGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTTACTTTTGCCGCTGGACGTTCTTTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTTTTCCGGCGT‑CCGGCTGTTGTAAATTTTGTTCTTGGTTTCCA‑TTTGCGCCAGCATGTTAGC‑TTTTCGCTGTTTTTCCCTTTTTTGGTTCTGGGCTTCACCTTTTACTTTTTCGAAGGTa                                                                                                                                                       >  1:863742/1‑239 (MQ=255)
                                                                                      gACCGGCGGTCTT‑TAGTTTTTTTGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTTCCGCTGGACGTTCTGTC‑AGAAGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCCGCGT‑CCGGAGGTTTTAAATTTTGTTTTGGGTTTTCC‑TGTTCGCCAGCATGTTTGC‑TTTTCGCTTTTTTTCCATTTTTTCGGTCTTCCCTTCAGCttttttttttttagagggaa                                                                                                                                                      >  2:1085902/1‑222 (MQ=255)
                                                                                      gACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGG‑CCGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATTTTCGCTTCAGCTTTTACTTTTTCTAAGGTAt                                                                                                                                                      >  1:1099406/1‑238 (MQ=255)
                                                                                      gACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAACATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTTTGGGTTTCCA‑TTGGCGCCAGCATGTTAGC‑TTTTCGCTGTTCTTCCATTTTTGCGGTCTTCGCTTCAGCTTTTACTTTATCGAAGGTAt                                                                                                                                                      >  1:543887/1‑238 (MQ=255)
                                                                                      gACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GGTGCAGGCATTTTTACTTTTTCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTTTGTTCCGGCGG‑CCGGTTGTTGGAAATTTTTGTTTTGGTTTCCC‑TGTGGGCCAGCCAGGTAGA‑TTTTCGCTTTTTTTTCATTTTTTCGATCTGCGCTTTAGCTTTTATTTTATCGAAggggta                                                                                                                                                     >  2:370180/1‑235 (MQ=255)
                                                                                         cggcggTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCt                                                                                                                                                  <  1:565345/239‑1 (MQ=255)
                                                                                            gggtttttattttttttGGCTTAAAGATTATTCAACA‑GTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCCGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTgg                                                                                                                                                <  2:615616/228‑1 (MQ=255)
                                                                                               tCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                             <  1:345574/238‑1 (MQ=255)
                                                                                               tCTT‑AAGTTTTTTGGCTGAAAGATTAATCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCaa                                                                                                                                             <  1:850006/238‑1 (MQ=255)
                                                                                                ttt‑AAGTTTTTTTGGTTAAAGATTATTCAGCA‑GTTGTATGCATTTTTCCTTTTGCCGGTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  2:180373/238‑1 (MQ=255)
                                                                                                ttt‑AAGTTTTTTGGCTGAAAGTTTATTCAGCA‑GTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  2:543887/238‑1 (MQ=255)
                                                                                                ttt‑AAGTTTTTTGGCTGAAAGATTTTTCAGCA‑GTTGCAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  2:258688/238‑1 (MQ=255)
                                                                                                cTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  1:142646/239‑1 (MQ=255)
                                                                                                cTT‑AAGTTGTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  1:349983/239‑1 (MQ=255)
                                                                                                 tt‑AAGTTTTTTGGCTGAAAGTTTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGGCGCTGGTCGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  1:727850/238‑1 (MQ=255)
                                                                                                 ctttatttttttGGGTTAAAGATTTTTCAGCC‑GTTGCAGGCATTTTTCCTTTTGCCGTTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAatg                                                                                                                                           <  2:1099406/233‑1 (MQ=255)
                                                                                                          tttGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TTTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGGATCGCTGGCAATGATGTCTTGc                                                                                                                                  >  1:431016/1‑239 (MQ=255)
                                                                                                                 gAAAGTTTTTTCAGTA‑GTTTGAGGCATTTTTCCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                                                                                                                           <  2:627361/239‑1 (MQ=255)
                                                                                                                  taaatgttttttcgccgtggCAGGCTTTTTTCCTTTTGCCTCTGGACTTTCTGTC‑AGACGCTTCTCAAAATTAGCTTTAAATGGCTTTTTCTGTTCCGGCGT‑CAGGATGTTTTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTgcgc                                                                                                                           <  2:577710/220‑1 (MQ=255)
                                                                                                                  aaaGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTATATTGCTTTTTCTGTTCCGGTGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCTCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGTCCTGCGCTTACGCTTTTCCTTTATCGAAGGTAACACTGCCatagttttttgcccttggcag                                                                                                                          >  2:1063730/1‑219 (MQ=255)
                                                                                                                      attattCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGt                                                                                                                      <  1:1085902/239‑1 (MQ=255)
                                                                                                                             agca‑gTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCa                                                                                                               >  1:710458/1‑239 (MQ=255)
                                                                                                                                   ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTTTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                                                              <  2:271669/171‑1 (MQ=255)
                                                                                                                                   ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCtt                                                                                                                                                                              >  1:271669/1‑171 (MQ=255)
                                                                                                                                   ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggc                                                                                                          >  1:734860/1‑239 (MQ=255)
                                                                                                                                   ttGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGAGATCTGAGCTTCAGCTTTTACTTTATCGAAGGCATCGCTGGCAATGATGTCATGCATTTCGCGGCGTTCTTCCatccgc                                                                                                          >  1:297651/1‑234 (MQ=255)
                                                                                                                                    tGCTGGCTTTTTTCCTTTTGCCGCTGGACTTTCTTTC‑AGACGCTTTTCAAAATTGACATTAAATTTCTTTTTCTGTTCCGGCGT‑CAGGATTTTTTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCTCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAgcggcg                                                                                                         <  2:781501/239‑1 (MQ=255)
                                                                                                                                     gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                                                                                                                           >  1:814207/1‑140 (MQ=255)
                                                                                                                                     gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTgcg                                                                                                                                                                                                           <  2:814207/140‑1 (MQ=255)
                                                                                                                                     gCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGcagcgg                                                                                                        >  1:928058/1‑239 (MQ=255)
                                                                                                                                           aTTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCATCGCCGGACGttt                                                                                                  >  1:497480/1‑239 (MQ=255)
                                                                                                                                             tttACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCACTGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGCCGTTTCa                                                                                                >  1:1058297/1‑239 (MQ=255)
                                                                                                                                                    tttGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGt                                                                                          >  1:790153/1‑238 (MQ=255)
                                                                                                                                                     ttGCCGCTGGCCGATCTGTC‑AGACGATTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAACTTTTGTTCTGGGTTTCCA‑TTTGCGCCCGCATGTCAGC‑TTTGCGCTGTTCTTCCTTTTTTGCGATCTGCTCCTCAGTTTTTCCTTTCACGAAGGTATCGCTTGCAATCATGCCACGCTTTCTTAGTCGTTCTTCCAGCTCCCGACGTTTCACTTGGTc                                                                                         >  2:377033/1‑238 (MQ=255)
                                                                                                                                                       gCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACg                                                                                      <  1:240565/239‑1 (MQ=255)
                                                                                                                                                                           gACGCTTCTCAAAATTAGGATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                   <  1:165293/239‑1 (MQ=255)
                                                                                                                                                                            aCGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCCTGATTTcgg                                                                  >  2:326258/1‑238 (MQ=255)
                                                                                                                                                                             cgtttCTCAAAATTAGCATTAAATTTCTTTTTCCGTGCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTATC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATTATTTcg                                                                   <  1:1063730/234‑1 (MQ=255)
                                                                                                                                                                             cGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTcg                                                                   <  1:78995/237‑1 (MQ=255)
                                                                                                                                                                                  ctcAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCTAAGGTATCGCTGGCAATGATGTCATGCATTTCGCGGCGTTCTTCCATCGGCGGACGTTTCATCTGGTCCCGCCGGCCTTTCATAATTTCggggtacg                                                            >  2:1037434/1‑235 (MQ=255)
                                                                                                                                                                                  ctcAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGAGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCGGCGCTTCAGCTTTTACTTTATCGAAGGTTTCGCTGGCCATGATGCCATGCATTGCGCGGCTTTCTTCCAGCGCCGGCGGTTTCATCTGGTCACGCTGGGCTTTctattttttccggtact                                                            >  2:623380/1‑223 (MQ=255)
                                                                                                                                                                                   ctaaaaTTAGCATTAAATTGCTTTTTCGGTTCCGGCGT‑CCGGATGTTGTAAATTTTGTTCTGGGTTTTCA‑TGGGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATctg                                                           <  1:1037434/237‑1 (MQ=255)
                                                                                                                                                                                                 aatttGCTTTTTCTTTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCAGTTTTGCGATCTGCGCTTTAGCTTTTACTTGATCGAAGGTATCGCTGGCCATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGt                                             <  2:263438/236‑1 (MQ=255)
                                                                                                                                                                                                  aaTTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGCTTTCGCGGATCTGCTGTTTCTGCGCGTc                                            >  1:597432/1‑239 (MQ=255)
                                                                                                                                                                                                      tcTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGGCCCAGCATTTTAGC‑TTTGCGCTGTTTCTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc                                        <  1:265947/238‑1 (MQ=255)
                                                                                                                                                                                                      gCTTTGTCCGTTTCGGCCT‑CATGGTGTTGGTAATTTTGTTCTGGGTTTCCA‑TGTGCGCCATCATGTTAGC‑TTTGCGCTGTTCGTCCATTTTTGCGATCTGCTCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCGATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtc                                        <  1:67682/239‑1 (MQ=255)
                                                                                                                                                                                                       cTTTTTCTGTTCCGGCGG‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTCCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCCCGGGTATGCTGTTTCTGCGGGTggggcg                                       >  2:1053395/1‑236 (MQ=255)
                                                                                                                                                                                                           ttttGTTCCGGCGT‑CGGGATGGTGTAAATTTTGGTGTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTTGTCTTCCATTTTTGCGATCCGCGCTTCAGCTTTTACTTTATCGAAGGTATCGGTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggtt                                   <  2:790153/236‑1 (MQ=255)
                                                                                                                                                                                                             cttttCCGGCTT‑TCGGTTGTTTTTAATTTTTTTTTGGGTTTCCG‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGTTCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttca                                 <  2:411123/236‑1 (MQ=255)
                                                                                                                                                                                                             cTGTTCCGGCGT‑TTGGTGTTTTTAAATTTTTTTGTGGGTTTCTG‑TGTGCGGCAGCATGTTAGC‑TTTGCGCTTTTCTTCCATTTTTGCGATCTGCGCTTCCGCTTTTCCTTTAGCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGGCGTTTCATCTGGTCGCGCTGGCCGTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGtcaggttca                                 <  2:417443/239‑1 (MQ=255)
                                                                                                                                                                                                              cgtgttcggccgtaggtgttttgaaaTTTTTTTTTGGGTTTCCA‑TGTTCGCCAGCTTGTTTGT‑TTTGCGCTTGTCTTCCATTTTTGCGATATGCGCTTCAGCTTTTCCTTTATCTAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGTATCTGCTGTTTCTGCTCGTCGGtcaggttca                                 <  2:928058/216‑1 (MQ=255)
                                                                                                                                                                                                                      cGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTg                         <  1:445767/238‑1 (MQ=255)
                                                                                                                                                                                                                       gT‑CAGGATGTTGTAAATTTTTTTCGGGGTTTCCA‑TGTGCGCCAGCAGGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGTAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGa                        <  1:577844/238‑1 (MQ=255)
                                                                                                                                                                                                                            ggATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAAcatc                   <  1:964428/239‑1 (MQ=255)
                                                                                                                                                                                                                                  tgtAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTTGTCACGCTGGCCTTTCATGATTTGCCGGCTCTGCTGTTTATGGCCGCCGGTCAGGTTTAGGTCTTTGACcaccttttcc             >  2:1091129/1‑231 (MQ=255)
                                                                                                                                                                                                                                    tAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCTCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTTAACATCATgc               >  1:23760/1‑234 (MQ=255)
                                                                                                                                                                                                                                          ttGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCGTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGGTTGATCTTGTCACGTTGTCCTTTCCTGATTTGCCGGATCTGCTGTTTCTGGGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGTAGCgg     >  2:278297/1‑239 (MQ=255)
                                                                                                                                                                                                                                             ttCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGAcc  >  1:330987/1‑239 (MQ=255)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              
GATCAGGCTTTTTTTACAACTGTTTGAG‑AAGAGAAAAGAAAA‑CCGCCGATCCTGTCCACCGCATTACTGCAAGGTAGTGGACAAGACCGGCGGTCTT‑AAGTTTTTTGGCTGAAAGATTATTCAGCA‑GTTGCAGGCATTTTACCTTTTGCCGCTGGACGTTCTGTC‑AGACGCTTCTCAAAATTAGCATTAAATTGCTTTTTCTGTTCCGGCGT‑CAGGATGTTGTAAATTTTGTTCTGGGTTTCCA‑TGTGCGCCAGCATGTTAGC‑TTTGCGCTGTTCTTCCATTTTTGCGATCTGCGCTTCAGCTTTTACTTTATCGAAGGTATCGCTGGCAATGATGTCATGCATTGCGCGGCGTTCTTCCAGCGGCGGACGTTTCATCTGGTCACGCTGGCCTTTCATGATTTCGCGGATCTGCTGTTTCTGCGCGTCGGTCAGGTTCAGGTCTTTGAACATCATGTCCTGATGCGGACC  >  NC_000913/1825024‑1825493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A