Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A8 F110 I1 R1 9 45.0 1568152 94.0% 1474062 142.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,186,085 C→A P246Q (CCG→CAG)  rpoC → RNA polymerase, beta prime subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,186,0850CA95.3% 122.1 / NA 44P246Q (CCG→CAG) rpoCRNA polymerase, beta prime subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (23/18);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTA  >  NC_000913/4185954‑4186224
                                                                                                                                   |                                                                                                                                           
tGAAGAGCTGATCGAAACCATCGCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGc                                                                                                                                  <  2:106174/143‑1 (MQ=255)
      gCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGc                                                                                                                            >  1:687313/1‑143 (MQ=255)
       cTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCGGCGTCCGCt                                                                                                                           >  1:86467/1‑143 (MQ=255)
          atcGAAACCAACTCCGAAACCAAGCGTAACAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTGCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGt                                                                                                                        <  1:760446/141‑1 (MQ=255)
                aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCt                                                                                                                  >  2:775069/1‑143 (MQ=255)
                   aaCTCCGAAACCAAGCGTAATAAGCTGACCAAGCGTATCAAACGGCTGGAAGCGTTCGTTCATTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGa                                                                                                               <  1:392764/143‑1 (MQ=255)
                      tCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGGCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtgg                                                                                                            >  1:293313/1‑143 (MQ=255)
                       ccGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTCCTGCCGGTCCTGCAGCCACATCTGCCCCCGCCGGTTCCGCTGGAtggt                                                                                                           >  2:511217/1‑143 (MQ=255)
                        cggacccAAGAGTAAAAAGCGGACCAAGCGGATCAAGCTGCTAGCGGCGTGCTGTCAGTCTGGTACCCAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtggtg                                                                                                          <  1:739990/138‑1 (MQ=255)
                        caaaaCCAAGCGTAAAAAGCTGACCAAGCGGAGAAAACGGCTGGAAGCGTCCGTTCAGTCGGGTAACAAACCAGAGTGGATGATCCTGACCGTTCCGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtggtg                                                                                                          <  1:66603/141‑1 (MQ=255)
                           aaCCACTCGTAGACAGCTGACCAGGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTc                                                                                                       <  2:780828/143‑1 (MQ=255)
                            aCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGGGGTCg                                                                                                      >  2:249457/1‑143 (MQ=255)
                             ccAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATATGCGTCCGCTGGTTCCGCTGGATGGTGGTCgg                                                                                                     >  1:761467/1‑142 (MQ=255)
                                   gTAAGAGGCGGACGAAGCGTATCAAACTGCTGTAAGCGTTCGTTCAGTCTGGTAACAGACCAGAGTGGATGGTCCTGACCGTTCTGCCGGTACTTCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTcgcg                                                                                               <  2:77791/143‑1 (MQ=255)
                                    tAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa                                                                                              <  1:128052/143‑1 (MQ=255)
                                       aaaGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACtt                                                                                           <  1:91294/143‑1 (MQ=255)
                                           cTGACCAAGCGTATCAAACTGCGGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGa                                                                                       <  1:96179/143‑1 (MQ=255)
                                                 aaGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGGCCTGaa                                                                                 >  2:264063/1‑143 (MQ=255)
                                                  aGCGTATCAAACTGCTTGAAGCGTTCGTTCCGGCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAAc                                                                                <  2:600974/143‑1 (MQ=255)
                                                      tATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGCTc                                                                            >  1:730649/1‑143 (MQ=255)
                                                      tATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATc                                                                            >  1:372495/1‑143 (MQ=255)
                                                       aTCAAACTGCTTGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGGTGATCATGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCt                                                                           <  2:238197/143‑1 (MQ=255)
                                                       aTCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCt                                                                           <  1:775069/143‑1 (MQ=255)
                                                           aaCTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTAt                                                                       <  1:550342/143‑1 (MQ=255)
                                                            aCTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGtctc                                                                      >  1:673433/1‑143 (MQ=255)
                                                                           cgtttATTCTCGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCTCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTTAACGATCTGTATCGTCGCGTCATTAAcc                                                       <  2:668632/143‑1 (MQ=255)
                                                                             ttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGATTCCGCCGGACCGAGGCCGATTCGCGACTTCTGACCTGGACGATCTATATCGTCGCGTCAGTCACCGt                                                     >  2:344855/1‑143 (MQ=255)
                                                                                  tCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCGGCGTCCGCCGCTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGGACGATCTGTATCGTCGCGTCATCAACCGTaccac                                                >  2:680383/1‑142 (MQ=255)
                                                                                   cTGGTAACAAACCAGAGTGGATGATCCCGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTaacaac                                               <  2:223481/143‑1 (MQ=255)
                                                                                          cacgccAGGGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTGCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGa                                        <  2:137016/139‑1 (MQ=255)
                                                                                           aagcccgagTGGATGATCCTGACCGGTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGaa                                       <  2:595515/137‑1 (MQ=255)
                                                                                                agagTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTc                                  >  1:629945/1‑143 (MQ=255)
                                                                                                    cgaatggtCCTGACCGCTGTTGCGGCACGGCCGCACGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTGTCTCGACTTCTGACGTGAACGGTCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTctgct                              <  2:354641/140‑1 (MQ=255)
                                                                                                      gatgatCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTgg                            >  2:398481/1‑143 (MQ=255)
                                                                                                        tgatCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGCCTTCTGACCTGAACGATCTGTATCGTCGCGTCATTACCCGTAACAACCGTCTGAAACGTCTGCTGGAt                          >  2:395461/1‑143 (MQ=255)
                                                                                                         gatCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCGTTAACCGTGAAAACCGTCTGAAACGTTTGCTGGATc                         >  2:487144/1‑143 (MQ=255)
                                                                                                           tCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTg                       >  1:304157/1‑143 (MQ=255)
                                                                                                                      cggCCGGTACTGCGTGCAGCTCTGCGTGCGCCGGTTGCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGa            <  2:358766/141‑1 (MQ=255)
                                                                                                                      cTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGa            >  2:206212/1‑143 (MQ=255)
                                                                                                                        gCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTCTCGTCGCGTCATTAACCGTAACAACCGTCGGAAACGTCTGCTGGGTCTGGCTGCGCCGGGca          >  1:395868/1‑143 (MQ=255)
                                                                                                                         ccGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTACCAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCgtctat         >  1:400697/1‑138 (MQ=255)
                                                                                                                          cGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGAcatc        >  1:590522/1‑143 (MQ=255)
                                                                                                                                tGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCCCCGGACATCCTcgtc  >  1:41140/1‑142 (MQ=255)
                                                                                                                                tGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGGCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGGTCTGACTGCGCCgggcgacagcggc  >  1:41025/1‑132 (MQ=255)
                                                                                                                                   |                                                                                                                                           
TGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATCGTA  >  NC_000913/4185954‑4186224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A