Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A8 F110 I2 R1
|
8 |
55.0 |
1914758 |
94.7% |
1813275 |
142.7 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
4,186,085 |
C→A |
P246Q (CCG→CAG) |
rpoC → |
RNA polymerase, beta prime subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 4,186,085 | 0 | C | A | 97.9%
| 135.3
/ NA
| 47 | P246Q (CCG→CAG) | rpoC | RNA polymerase, beta prime subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (21/25); minor base G (0/1); total (21/26) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATC > NC_000913/4185952‑4186221
|
cattaaGAGCTGAACGAAAACAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCt < 2:841362/139‑1 (MQ=255)
gTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGCATGATCCTGACCGTTCTGCCGGTACTGCAGCAAGATCTg > 1:90898/1‑143 (MQ=255)
tGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGc < 1:850607/143‑1 (MQ=255)
gcaaaaggtGAACGAAACTAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGc < 2:840891/135‑1 (MQ=255)
cTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCt < 2:940575/143‑1 (MQ=255)
aaCGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTATGGTAACAAACCAGAGCGGATGATCCTGACCGTTCTGCCGGTACCGCAGCCAGATCTGCCTACGCTGGt > 1:133632/1‑143 (MQ=255)
aCCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCt < 2:151276/143‑1 (MQ=255)
cgaCTCCGAAGCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGGAACAAACCCGAGTACTTTATCCTGTCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTgg < 2:548651/141‑1 (MQ=255)
ccGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAtggt > 2:925564/1‑143 (MQ=255)
ccGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGGTCCGCTGGAtggt > 2:173437/1‑143 (MQ=255)
gAAACCAAGCGTAAAAAGCTGACCAATCGTATCAAACTGCTAGAAGAATTCGTTAAGTCTGGTAACAAACAAGAGTGGATGATCCTGACCGTCCTGCAGGAACTGCAGCCAGATCTCCGTCCCCGCGTTCCTCTGGAtgcggg > 1:67028/1‑139 (MQ=255)
aaaCCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATggtggt > 2:674206/1‑143 (MQ=255)
cGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTcgc < 2:430231/143‑1 (MQ=255)
tAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa < 2:799834/143‑1 (MQ=255)
tAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa > 2:240028/1‑143 (MQ=255)
gaaaaaGCTGACCAAGCGTATCACACTGCTGGAAGCGTTCTTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCACAGCTTCTTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGa < 1:291918/142‑1 (MQ=255)
cTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGa < 2:50889/143‑1 (MQ=255)
cTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTGCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGa < 2:513946/143‑1 (MQ=255)
gACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGCCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGAcc > 2:209482/1‑143 (MQ=255)
aCCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGGTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGAGACTTGTGGCCt > 1:301872/1‑143 (MQ=255)
cAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCGGa > 2:921867/1‑143 (MQ=255)
aaGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCCGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGAGGGTGGTCGTTCCGCGACTTCTGGccatga > 1:88599/1‑139 (MQ=255)
tCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGCTGGTGGTCGTTTCGCGAATTCTGACCTGAACGATCTg > 1:750568/1‑143 (MQ=255)
cAAACTGCCGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGt < 2:92228/143‑1 (MQ=255)
tgGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTcgc < 2:545799/143‑1 (MQ=255)
ggAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTcgcg < 1:407192/143‑1 (MQ=255)
aGCGTTCTTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCGGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCa < 2:940115/143‑1 (MQ=255)
gcgtgcggggggggtGGTGACAGACGGGAGTGGAGGATCCTGGCCGTGCGGCCGGTACTGCGGCCAGATCTGCGTCGGCTGGTTCCGCTGGATGGTGGTCGTGTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCAt < 1:28499/129‑1 (MQ=255)
cgttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAAcc < 2:335798/143‑1 (MQ=255)
gttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGCCCTGCACGATCTGTATCGTCGCGTCATGAACCg > 1:235127/1‑143 (MQ=255)
ttcAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGt > 2:621534/1‑143 (MQ=255)
cattCTGGTAACAAGTTAGAGTGGTTGATGCTGATCGTTCTGCCGGTACTGCAGCCAGATCTGCGTTCGTTTGTTCTGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTaa < 1:541076/140‑1 (MQ=255)
gTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGAAACAAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCATTTCGCGACTTCTGACCTGAACGATCTGTACCGGCACGTCATGAAcctgagaa > 1:99017/1‑137 (MQ=255)
tCTGGTAACGAGCCTGAGTGGATGAGCCTGACCGTTCTGCGGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTaacaa < 1:300557/143‑1 (MQ=255)
gTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGt > 2:826416/1‑143 (MQ=255)
aCCGGAGTGGATGATCCGGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAAc < 2:492157/143‑1 (MQ=255)
aCCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAAc < 2:722455/143‑1 (MQ=255)
cAGAGTGGATGTGCTTGACCGGTCTGCCGTTACTGCAGCCAGAGCTGCGTCCGCGGGTTCCGCTGGATGGTGGTCGGTTCGCGAGTTCTGACCGGAGCGATCTGTATCGTCGCGTCATTAACCGTGACAACCGTCTGAAACGt < 1:6187/143‑1 (MQ=255)
agTGGATGATCCTGTCCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTctg < 1:393375/143‑1 (MQ=255)
tgatCCTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCAACTTCTGACCTGCAGGATCTGTATCGTCGCCTCCTTAAACGTAACAACCGTCTGAAACGTCTGCAGGaa > 1:61804/1‑142 (MQ=255)
gatCCTGACCGTTCTGCCGGTACTGCAGCCAGAACTGCGTCCGCTGGTTCCGCTGGATGGTGATCGTTTCGCGACTTCTGACCTTAACGATCTGTCTCGTCGCGTCATTAACCGTCACAAGCGTTTGCAACGTCTGCTGGATc > 2:631030/1‑143 (MQ=255)
cTGACCGTTCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAATGATCTGTATCGTCGCGTCATTAACCGAAACAACCGTCTGAAACGTCTGCGGGAttttgc > 2:671143/1‑138 (MQ=255)
ttCTGCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCg < 1:842999/143‑1 (MQ=255)
cggCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGa < 2:525575/141‑1 (MQ=255)
gCCGGTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCACGACTTCTGACCTGAACGATCTGTATCGTCGAGTCATTAACCGTAACAACAGTCTGAAACGTATGCTGTGTCTTAATGAGCCGGAca > 1:727825/1‑143 (MQ=255)
ggTACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGCACGCCCTGTATCGTCGCGTCATTCACTGAAAAAATCGTCCGAACCGACTGCTGGCTCTGGCTGCGCCGGAcatca > 1:567280/1‑143 (MQ=255)
tACTGCAGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTCTCGCTACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACatcatc < 1:674206/143‑1 (MQ=255)
|
CGTGAAGAGCTGAACGAAACCAACTCCGAAACCAAGCGTAAAAAGCTGACCAAGCGTATCAAACTGCTGGAAGCGTTCGTTCAGTCTGGTAACAAACCAGAGTGGATGATCCTGACCGTTCTGCCGGTACTGCCGCCAGATCTGCGTCCGCTGGTTCCGCTGGATGGTGGTCGTTTCGCGACTTCTGACCTGAACGATCTGTATCGTCGCGTCATTAACCGTAACAACCGTCTGAAACGTCTGCTGGATCTGGCTGCGCCGGACATCATC > NC_000913/4185952‑4186221
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
