Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F27 I0 R1	322	127.1	2757226	91.8%	2531133	223.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,274,347	T→G	20.7%	G57G (GGT→GGG)	yejB →	microcin C ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,274,347	0	T	G	20.7%	179.4 / 5.6	88	G57G (GGT→GGG)	yejB	microcin C ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (16/47); new base G (16/7); total (34/54)
Fisher's exact test for biased strand distribution p-value = 3.15e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGC  >  NC_000913/2274113‑2274576

tACCTGGTGGTAGGACGTCAATAAAGCGGCCAAACTGCCTTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGtt                                                                                                                                                                                                                                      <  1:1210311/238‑1 (MQ=255)

    tggtggTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGGCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCCCCGCCGGTG‑GAGGGGTTtgtg                                                                                                                                                                                                                                  >  2:265613/1‑238 (MQ=255)

    tggtggTATGACGTCAATAAAGCGGCCAAACTACCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGCCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGGGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGTg                                                                                                                                                                                                                                  >  1:1276042/1‑238 (MQ=255)

        ggtATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCTGg‑ccag                                                                                                                                                                                                                             >  1:1049429/1‑238 (MQ=255)

              ccggtcaaaacgCGGCCAAACGGCCGTCCGCCAGAAAACAGGGGCA‑GTAGGTGGGTGCTTACCTGGTTCGCCGTCTGTTGCTGGGGATCCCAACATTGTGGGCGATTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATgcg                                                                                                                                                                                                                      <  2:1071354/228‑1 (MQ=255)

                gTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGG‑AAGGGGTTCTGG‑CCAGCCATggcca                                                                                                                                                                                                                    >  1:315683/1‑235 (MQ=255)

                   aaaaaggCGGCCACCCTGCGTCCCGCCAAAAAACAGGGGGA‑TTAGATGGCCGCTTCCCTGTTTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCaaa                                                                                                                                                                                                                  <  2:73807/232‑1 (MQ=255)

                       agcgggccaacccgcgccccGCCAGCAAAAAGGGGGG‑GTAGATGGGCTCTTCCCTGATTCGCCTTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGgt                                                                                                                                                                                                             <  2:1049429/221‑1 (MQ=255)

                        aGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGGGATCCCAACATTATGGGCGGTTATCACCCTCAAATTTTTCATCGTG‑CCAATTGCGCCTGGCGGTCCGGTCGACCAGGCCACCGCCGCCATTTAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGG‑AAGGGGTT‑GTGTACAGCCAAGGGCAAACcgggg                                                                                                                                                                                                            >  2:438656/1‑237 (MQ=255)

                             ccAAACTGCCGTCCGCCAGCAAACAGTGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAACTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGCGTATTACCCGGCGCAGGCGGTG‑AAAGGGTTCGTG‑CCAGCCACGAGCAAACGGGTGTCGGc                                                                                                                                                                                                       >  1:287688/1‑239 (MQ=255)

                             ccAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGGTCCTG‑CCAGCCCAGCGCAAACGCGGGTccgg                                                                                                                                                                                                       >  1:1196378/1‑236 (MQ=255)

                                       gTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTTCCCGGCGCAGGCGGTG‑AAGGGGTTGGTG‑CCCGCCAGGCGCAAACGGGGGTGGGCAAAAACAGCg                                                                                                                                                                                             >  2:114314/1‑239 (MQ=255)

                                         cccccccgaaaCAGGGGGGGGTAGTTGGGCCGTTACCGTATTCCCCGTTTGTTCGTGGTGGTCCCAACATTTTGGGCGATTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGa                                                                                                                                                                                            <  2:1324729/231‑1 (MQ=255)

                                         ccccccagaaaCTGGGGGG‑GTGGGTGGGCGTTTTCCTGTTTCGCCGGTTGTTTATGGTGAACCCAACATTATGGGGGATTTACCCCCACAACTTTTTTATCGTG‑AAAATTGCGCGTGGCGGGCGGGTCGACCCGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                           <  2:766484/231‑1 (MQ=255)

                                         cccccAGCAAAGGGGGGGA‑TTAGGTGGGGCTTTTCCTTATTCCCCGTCTGTTGCTGGGGATCCCAACATTATGGGCGATTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGAc                                                                                                                                                                                           <  2:332369/236‑1 (MQ=255)

                                               gCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTAACCGGCGCAGGCGGTG‑AAAGGGTTCGTG‑CAAACCATGCGCAAACGGG‑GTCgggcaaattacacgccaggaat                                                                                                                                                                                    >  1:102489/1‑219 (MQ=255)

                                                               tggtGGGGGTTTCCCTGCTTCGCCTTTTTTTTCGGGTGCCCCCAACTTTATGGGCTATTTGCCCCATCAATTTTTTCATCGTG‑CAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                       <  2:599379/235‑1 (MQ=255)

                                                               aGATGGGGGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAcg                                                                                                                                                                       <  1:1353135/238‑1 (MQ=255)

                                                                gATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGTTTCGGG‑CCAGCCAACCCCAAACCGGTGTCGGCAAAATACGGGACAGGAAATACCGggggggcggcg                                                                                                                                                                     >  2:170513/1‑234 (MQ=255)

                                                                  tgggcgtttttctttttCGCCGTCTTTTGGTTGTGACCCCAACATTATGGGCGTTTATCCCCATCAACTTTTTCACCGTG‑CAATTTGCGCCTGGCGGTCCGGGCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGa                                                                                                                                                                   <  2:23286/224‑1 (MQ=255)

                                                                  tGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCCTTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑ACGGCGTTCGTG‑CCACCCCAGCGCAAACGGGGGTCGGGCATATCAGCGCCCGTAATTACCCTGGCGGACACggg                                                                                                                                                                   >  2:255463/1‑238 (MQ=255)

                                                                   gggCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGGG‑AAGGTGGTTGTG‑CCAGGCCTGCGCAAACGGGGGTCGGCCATATCAGCGCGAGGAATTACCGTGGGGGGCgggggt                                                                                                                                                                  >  2:1162952/1‑237 (MQ=255)

                                                                    ggtgtttccttttttCCCCGTCTTTTTTGGGTGTACCCAACATTTGTGGCGTTTCTCCCCTCAAACTTTTTCATCGTG‑CAAATTGCGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  2:182168/226‑1 (MQ=255)

                                                                     gtccTTACCTGATTCGCCTTCTATTGCTGGTTATCCCAACATTATGGGCGATTATCACCATCAACTTGTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:733349/235‑1 (MQ=255)

                                                                     gcgcTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:186243/238‑1 (MQ=255)

                                                                     gcgcTTACCTGATTCGCCGTCTGGTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  1:750381/238‑1 (MQ=255)

                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGTGCGGACGCGGATTAGa                                                                                                                                                              >  1:1065802/1‑238 (MQ=255)

                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGTG‑CCCGCCATGCGCAAACGGGTGTGGGCAAAATCAGCGACAGTAATTACCGTGGCGGagccgggtttga                                                                                                                                                              >  1:1110620/1‑227 (MQ=255)

                                                                        cTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGGG‑CCAGCCATGGGCAAACGGGGGTCGGCAATATCAGCGACCGTAATTACCGTGGCGGACCCGGGTTAGa                                                                                                                                                              >  1:917526/1‑238 (MQ=255)

                                                                           aCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGGGTCGGCAAAATCAGCGACAGTAATTACAGTGGCGGACGCGGAGTAGATCCa                                                                                                                                                          >  1:1128057/1‑239 (MQ=255)

                                                                           aCCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGCGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGAAATTACCGTGGCGGACCCGGATTAGATCCa                                                                                                                                                          >  1:681140/1‑239 (MQ=255)

                                                                                     ccGTTTTTTTCTGGTGTTCCCAACATTTGGGGCGTTTATCCCCATCAACTTTTTCACCGGG‑CAAATTGCCCCTGGCGGGCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCg                                                                                                                                                <  2:1276235/239‑1 (MQ=255)

                                                                                       gTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCCTGGCGGACCCGGATTAGAACCCGaaaccggttct                                                                                                                                              >  1:430590/1‑230 (MQ=255)

                                                                                        tCTATTGCTTGTGATCCCAACAGTATGGGCGATTATCACCATTAACTTTTTCATCGTG‑CAAATTGCGCCGGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGccg                                                                                                                                             <  1:886108/239‑3 (MQ=255)

                                                                                                ttgttacccccACTTTGTGGGCGATTATCACCCTCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACt                                                                                                                                     <  2:244839/232‑1 (MQ=255)

                                                                                                  gTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCCGGCGGTG‑AAGGGGTTCGTG‑CCAGCCCTGCGCCAACGGGGGTCGGCACTATCCGCCACAGGAATTACCGTGCGGGGCGCGGGTTTGATCCCAAAAGGATGGCTgagcgaaacc                                                                                                                                    >  2:694189/1‑231 (MQ=255)

                                                                                                   gttccccAACTTTTTGGGCGTTTATCCCCATCACTTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGGCCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                  <  2:895753/235‑1 (MQ=255)

                                                                                                    ggtCCCAACATTAGTGGCGATTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCAt                                                                                                                                  <  1:57686/236‑1 (MQ=255)

                                                                                                       cccAACATTCTGGGCTATTATCACCATCAACTTTTGCATCGGG‑AAAATTGCCCGTGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCt                                                                                                                              <  1:530299/239‑1 (MQ=255)

                                                                                                            cttttcgggcatttATCCCCATCAATTTTTTCACCGTG‑CAAATTGCGTCTGGGGGTCCGGTCGACCAGGCCATGGCCGCCATGGAGTTGGGTAAGGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGt                                                                                                                         <  2:681140/227‑1 (MQ=255)

                                                                                                            actttggggcttttttCACCCTCAACTTTTTCATCGTG‑CAAATTGGCCCTGGCGGTCCGGCCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGt                                                                                                                         <  2:1128057/224‑1 (MQ=255)

                                                                                                                 tGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCAATCATCGCTACGGGTTTGa                                                                                                                    >  1:493159/1‑239 (MQ=255)

                                                                                                                       aTTATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCAATCGCCGACATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGc                                                                                                               <  1:58590/238‑1 (MQ=255)

                                                                                                                       aTTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGGG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGCCAGAAATTACCGTGGGGGACGCGGGTTAGAGCCAGAAATGATGCCTGGGGTAACTCATCCCTCCGGTTTTGTAAAGcc                                                                                                              >  2:900663/1‑239 (MQ=255)

                                                                                                                        ttATCCCCATCAACTTTTTCATCGTG‑CAAATTGCGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                             <  1:119934/239‑1 (MQ=255)

                                                                                                                         ttttcccacttaCTTTTTTTTCCTGCCAAATTGCCCTGGGCGGGCCGGTGCACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCg                                                                                                             <  2:729307/228‑1 (MQ=255)

                                                                                                                             aCCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCCGAAGGGATAGCCGAGATCACTCATCGCACCGGGTTTGGTAAACCGAA‑cc                                                                                                        >  1:925118/1‑238 (MQ=255)

                                                                                                                                  cAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACCGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGGAGTGATCGCTGGAATCACTCCTCGCTACGGGTTTGATAAGCCGAG‑CCACGaa                                                                                                   >  1:18946/1‑238 (MQ=255)

                                                                                                                                   aaCTTTTTCATCGTG‑CAATTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACg                                                                                                 <  1:1162952/239‑1 (MQ=255)

                                                                                                                                    attttttctcgggg‑CAAATTGGGCCTGGCGGCCCGGGCCACCGGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGt                                                                                                <  2:917526/226‑1 (MQ=255)

                                                                                                                                         ttCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCCGCGACAGTAATTTCCGTGGCGGACGCGGGTTAGATCCCGAAAGGGTCGCTGAGGTCACCCAACCCCACGGGTTTGGTAAACCGATACCAC‑AACGGTACtt                                                                                           >  2:280299/1‑239 (MQ=255)

                                                                                                                                             ccGTG‑GAAATTGCGCCTGGGGGTCCGGTCGAACCGGCCATCGCCGCCATTGATTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCaaa                                                                                       <  1:1202007/238‑1 (MQ=255)

                                                                                                                                               ttgttaatttggccttGGGGGTCCGGTCGCGCATGCCAGCGCCGTCTTTTGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTT‑GAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCaaaa                                                                                      <  2:102489/224‑1 (MQ=255)

                                                                                                                                                tG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCAATGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGGCGGCAATATCAGCGACCGGAATTACCGCGGGGGAAGCGGGTTAGATCCAAAAGTGATCGCTTGGATCACTCATCGCCACGGTTTTGGTAAACAGAA‑CCACCaaacgaactgccaaaaa                                                                                    >  2:446735/1‑224 (MQ=255)

                                                                                                                                                     atttGGGCCGGGCGGTCCGGTCGACCGGGCCATCGCCGCCATTGGGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGctct                                                                                <  2:167134/237‑1 (MQ=255)

                                                                                                                                                     aaTTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGctct                                                                                <  1:694189/239‑1 (MQ=255)

                                                                                                                                                         gggcCTGGGGGCCCGGTCGACCAGGCCATGGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGa                                                                            <  1:280299/237‑1 (MQ=255)

                                                                                                                                                                gCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACAt                                                                      <  1:900663/238‑1 (MQ=255)

                                                                                                                                                                           cgccAGGCCATGGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGAtt                                                           <  1:255463/236‑1 (MQ=255)

                                                                                                                                                                                     tCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 >  1:280250/1‑127 (MQ=255)

                                                                                                                                                                                     tCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGAtt                                                                                                                                                                 <  2:280250/127‑1 (MQ=255)

                                                                                                                                                                                     cccccgccATTGTGTTTGGTAATGGTGGGGGTTACCCCGTGGCAGGCGGTG‑AAGTGGGTCTGG‑CCGGCCAGGCGCAAACGGGGGGCGGGAATATCAGCGACAGTAATTACCTGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGAACACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGc                                                <  2:1223671/236‑1 (MQ=255)

                                                                                                                                                                                      cgccgcCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGGGTTCGGG‑CCAGCCATGCGCAAACGGGGGGCGGCAATATCAGCGGCAGTAATTACCGGGGCGGACCCGGATTAGGTCCAGAAAGGATCGCTGAGATCACCCATCGCCCCGGTTTTTGTAAGACGAT‑TCCCCAACGGTAATTCCAAATGCCCTGGGACTACAACCGCTTTTGGTTTGGGGGTAGcc                                               >  2:634829/1‑239 (MQ=255)

                                                                                                                                                                                       gccgccATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGGCGCGGAGTAGATCCAGAAGGGATCGCTTAGATCACTCCTCGCTTCGGTTTTGGTAAACCGAT‑CCCCGAACCTTACTTCCAAATGGTCTGGGCCTACAAACCCTTTGAttttttggaaaaccc                                              >  2:120763/1‑228 (MQ=255)

                                                                                                                                                                                             aTTGAGTTTGGGAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCAAGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGGCGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCg                                        <  1:938578/239‑1 (MQ=255)

                                                                                                                                                                                                   tttGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAAAGCCAGAAGTGATCCCTGGGATAACTCAACGCCACGGTTTTGGAAAGCCGCT‑CCCAGAACGTTACTTCAAAAGGGCCTGGGACAAAATCCCCTTTTATTTTGGGGTTAACCTtttttgaacgg                                   >  2:1257699/1‑231 (MQ=255)

                                                                                                                                                                                                   tttGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAAGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTTGTAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTCAATCCGCTTTGATTTTTGCGTTAGCCTGTTTCCCaagg                                   >  1:924358/1‑235 (MQ=255)

                                                                                                                                                                                                     ttgTAATGCCGGGGTGTTCCCCGGCGGCGGGTGTG‑AAGGTGGTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                <  2:493159/237‑1 (MQ=255)

                                                                                                                                                                                                     tGGTAATGCCGGAGTATTTCCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCt                                <  1:114314/239‑1 (MQ=255)

                                                                                                                                                                                                           tGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCaaaa                                                                                      <  1:298348/179‑1 (MQ=255)

                                                                                                                                                                                                                gggtttttcccGGCGGAGGCGGTG‑AAGGGTGTCGTG‑CCCGCCAGGGGGAAACGGGTGTCGGCAATTTCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACg                     <  2:715666/231‑1 (MQ=255)

                                                                                                                                                                                                                  ttttttcccgggcggggCGGTG‑GAGGGTTTCGTG‑CCAGCCATGCGCAAACGGGGTTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:866367/223‑1 (MQ=255)

                                                                                                                                                                                                                  ttATTACCCGGGGCAGGGGGTG‑AAGGTGTTCGGG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  1:3891/237‑1 (MQ=255)

                                                                                                                                                                                                                  gttttccccggggacgggggTG‑AAGGGTTTCGGG‑CCAGCCAGGCGCAAACGGGTGTCGGCAATATCAGCGAAAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:759780/220‑1 (MQ=255)

                                                                                                                                                                                                                  gttttccccGGCGCGGGGGGTG‑AAGGGGTTCGGG‑CCAGCCAGGGGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:924358/232‑1 (MQ=255)

                                                                                                                                                                                                                  gTATTCCCCGGCGCGGGGGGTG‑TAGGTTGTCGGG‑CCAGGCATGGGCAAACGGGTGGCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCTCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGTCGc                    <  2:287688/238‑1 (MQ=255)

                                                                                                                                                                                                                  gTATTACCGGGCGGGGGCGGTG‑AAGGGTGTCGTG‑CCAACCATGGCCAAACGGGTTTCGGAAATATCAGCGAAAGTAATTCCCGTGGCGGACGCGGATTAGATCCAGGAGGGAGCGCTGAGATCACGCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  2:992145/238‑1 (MQ=255)

                                                                                                                                                                                                                  gTATTACCCGGGCCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  1:446735/238‑1 (MQ=255)

                                                                                                                                                                                                                  gTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGctct                                                                                >  1:1058203/1‑178 (MQ=255)

                                                                                                                                                                                                                  gTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGctct                                                                                <  2:1058203/178‑1 (MQ=255)

                                                                                                                                                                                                                  attcttgccgggtgaGGCGGTTAAAGGTG‑TCGCG‑TCAGCCATGCGCAAACGGGTGTCGGCCATATCAGCGACAGTAATTTCCGTGGCGGACGCCGATTAGATCCAGAAGTGATCCGTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGc                    <  1:386937/224‑1 (MQ=255)

                                                                                                                                                                                                                     ttccccGGGCCAGGCGGTG‑AAGGTGTTCGGG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGAt                <  2:1067915/236‑1 (MQ=255)

                                                                                                                                                                                                                       aCCCGGCGCAGGCGGTG‑AAGGTGTTAGTG‑CCAGCCATGCGCAAACGGGGGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGGTTAGATCCAGAAGTGGTCCCCGAGATCACTCCACGTTCCGGTTTTGATTACCCGCT‑CCACGACCGGTACTTCAAAATACTCTGGGACTACAACCCCTTTGATTTTTGGGATTACCTGTTTAGCCGCGCCCCgtgggtgacacgggtta              >  2:244666/1‑223 (MQ=255)

                                                                                                                                                                                                                           gggccAGGCGGTG‑AAGGGGTTCGTG‑CCAGCCATGCGCAACCGGGTGTCAGCAAAAACAGCGACAGGAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGa          <  1:1301729/235‑1 (MQ=255)

                                                                                                                                                                                                                             cgcAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCCAAATGCTCTGGGGCTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGGGCTGACGCTGATTTAAGACa        >  1:1067915/1‑239 (MQ=255)

                                                                                                                                                                                                                             cgcAGGAGGCG‑AAGGTGGTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGAAGCGGATTAGATCCAGAAAGGATCGCTTAGATCACTCATCGCCACGGGTTTTAATAGCCGAT‑CACCGACAGTTACTTCACAAAGCTCTGGGACTACATCCGCTTTGATTTTGGGGTTAACCTTTTTTCCAGCGCCACGCGGCGGGAGCGGATTaaaaaaa        >  2:1232401/1‑235 (MQ=255)

                                                                                                                                                                                                                                   ggggg‑AAGGTTTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGGGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTg   <  2:1110620/234‑1 (MQ=255)

                                                                                                                                                                                                                                    ggTT‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGc  <  1:68601/238‑1 (MQ=255)

TACCTGGTGGTATGACGTCAATAAAGCGGCCAAACTGCCGTCCGCCAGCAAACAGGGAGA‑GTAGATGGGCGCTTACCTGATTCGCCGTCTGTTGCTGGTGATCCCAACATTATGGGCGATTATCACCATCAACTTTTTCATCGTG‑CAAATTGCGCCTGGCGGTCCGGTCGACCAGGCCATCGCCGCCATTGAGTTTGGTAATGCCGGAGTATTACCCGGCGCAGGCGGTG‑AAGGTGTTCGTG‑CCAGCCATGCGCAAACGGGTGTCGGCAATATCAGCGACAGTAATTACCGTGGCGGACGCGGATTAGATCCAGAAGTGATCGCTGAGATCACTCATCGCTACGGTTTTGATAAGCCGAT‑CCACGAACGTTACTTCAAAATGCTCTGGGACTACATCCGCTTTGATTTTGGCGATAGCCTGTTTCGCAGCGCCTCGGTGCTGACGCTGATTAAAGACAGTCTGC  >  NC_000913/2274113‑2274576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A