Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F17 I0 R1
|
155 |
84.1 |
1797446 |
90.9% |
1633878 |
230.3 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,151,689 |
G→T |
53.1% |
intergenic (+43/+22) |
yegJ → / ← yegK |
uncharacterized protein/ser/thr phosphatase‑related protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,151,689 | 0 | G | T | 53.1%
| ‑5.1
/ 3.8
| 13 | intergenic (+43/+22) | yegJ/yegK | uncharacterized protein/ser/thr phosphatase‑related protein |
| Reads supporting (aligned to +/- strand): ref base G (4/2); new base T (5/2); total (9/4) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01 |
ACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGC‑GCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGC > NC_000913/2151486‑2151926
|
atgtaaaatggggCAAGGAGTGAAAAAACAAAAAAGAAAAT‑TTTCAGGCTGGTGGTTAACACGGGGGGGAAAAATTTTGCGGGGT‑TTTAACCTTTAATCTTTTATTGGTCCCCTTTCAAAAAAAAGAAGCCGCCGAGCAAAGAGAAAAATTAGTTCGCTAATCAGCGTTTATTTTTATTGTTAAAACG‑GGCGTTTAAAT‑TGTTGTCTT‑AAACCGCCATGAGAAACTCACTCTGCCcaca < 2:184374/228‑1 (MQ=255)
agaggtgtAAAAAAAAAAAAAAAAATTTTTTAGGCTGTATTTTTTCGC‑GGGGGGAAAAAATTTGCCGTGGTTTATACCTTTGATATTTTGGTGTTCACCAACAAAAAAGAATAACCCGAGGAGCAAAGGAAAAAATTGTTGCTCTAA‑CAACCGTTTTTTTTTTTTTTTAAAGTGGGGGGTTGATT‑GTTTGTCTC‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCaa < 1:693269/234‑1 (MQ=255)
ttGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTTTCTT‑CAACCGCCCTTGGAAAATCACCCTTGCCCCAGCGCCCAGGCCCACGGTTTTTACACATCCCTACCCCCGGTTT‑CCGCCCGGCTGGGCAAAAACATCTTTAACCATTTAGGGaaaaaaatcatgt > 2:614384/1‑232 (MQ=255)
tGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TGTTGTCTT‑TCACCGCCATGAGAAAACCACTCTTTCCACAGCGCCACTGCCCAAGCTTTATCATCTTCCGTACGCTCCCTTA‑CCTCCCGGGCGGGCACAAATACATTTAACAATTCAGGTAACAAATCgc > 2:827086/1‑234 (MQ=255)
aTCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGTGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGGAT‑TGTTTTCTT‑TAAACGCCATGGGAAACTCACTCTTCCCACAGCGCCAGTTCCAACGTCTTATCATTATTCTGACCCCCGTTTT‑TCGCCGGGGTGTGCAAAAACAGCTTTTACAATTCAGGCAATAAAtatcctttg > 2:832377/1‑230 (MQ=255)
ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTGAA‑TGTTGTCTT‑CAACCCCCCTGAGAAACTCACTCTTCCCCCAACGCCAGTGCCAACAGTTTTTTATCATCCGTACACCCGTTTACCCCCCGGGTGGGACAAAAACTCTTTTAACAATTCGGGAaaaaaaaacgagtttcccgc > 1:226889/1‑225 (MQ=255)
aCCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTTTCTT‑CAACCGCCATTAGAAAATCACTCTGCCCCCAGCGCCCGTGCCAACGGTTTATTCTCATCCCTACGCCCGTTTA‑CCGCCCGGCCGGGCAAAAACTGGTTTTACAATTCAGGTAATAAAACGAGGTGTTTCTCGGTtgctgc > 2:718140/1‑238 (MQ=255)
ccTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TTTTTTCTT‑CAACCGCCATTAGGAAATCACTCTGCCCCCAACGCCAGTGCCCAAACTTTATCATCATCCCTCCGCCCGCTTT‑TCGCCACGGCGGCAAAAAAATACTTTTACAAATACTGGAAAAAAACGAGATGTTCATACGTTGTTgccg > 1:130752/1‑239 (MQ=255)
cTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCCTTAGAAACTCACTCTTCCCACAGCGCCAGTGCCAACGGTTTATCATCATCCGTACGCTCGGTTA‑CCGCCGGGCTGGGCAAAAACTGCTTTTACAATTTAGGTAATAAATAGAGTTGTTTCTGCGGTTCTgccg > 2:135487/1‑238 (MQ=255)
tgttcgcaaaatcgcctttttttttattTTTTAAAGT‑GGGGGTTTAAT‑TGTTTTTTTAAAACCCCCATGAAAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATgag < 2:778136/217‑1 (MQ=255)
tCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTTAAT‑TGTTTTCTT‑CAACCTCCCTGAGAAACACACACTGCCCCCAGCGCCCGTGCCCACGTTTTTTCATCATCCGTACCCCCGCTTA‑CCTCCGGGCTGGCCAAAAAATTCTTTAACAAATCACGTAATAAAAAGCGTTGTTCCTGCGTTTCTGCGCCCAGTCCCTTGAAAAAAGGGGGaaaaaaaaaaaaaaagggaga > 1:856347/1‑225 (MQ=255)
cTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TTTTGTCTT‑CCACCGCCATGAGAAAATCACTCTTCCCACAGCCCCCAGGCCAAACTTTTTTCATCATCCGCAAGCTCGTTTT‑CCGCCCGGCTGGCCAAAAAATGCTTTTAAAAATCACGTAATAAAACCGGTTGTTCCTGCGTTGGTGCCCCCCGCCCATTGAAAAAAGGGGGAAAAAAAGGAACAATAGGGGaaaa > 1:778136/1‑237 (MQ=255)
tgttAAACT‑GGGCGTTTATTGTGTTTTTTG‑CAACCGGCATGGGAAACTCACTCTGCCCACAGCCTCAGTTCCAACGTTTTATCCACATCCGTACGCTTGGTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAg < 2:856347/237‑1 (MQ=255)
ggtttaattttttgtGTC‑CACCCGCCATGGAAAACTCCCTCGGCCCCCAGCGCCAGGGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATAc < 2:596470/227‑1 (MQ=255)
gtCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGCGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGTTACCATCCGTAAAtgc > 2:413218/1‑238 (MQ=255)
|
ACGTAGAATATGGCCAGGAGTGGAAAATCAAGAAAGAAGATATTTCAGACTGGATGTATACGC‑GCGGGGATAAAATTTACGGCGG‑TTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACT‑GGCGGTTGAAT‑TGTTGTCTT‑CAACCGCCATGAGAAACTCACTCTGCCCACAGCGCCAGTGCCAACGTTTTATCATCATCCGTACGCTCGTTTA‑CCGCCGGGCTGGACAAAAACTGCTTTAACAATTCAGGTAATAAATCGAGTTGTTCCTGCGTTGCTGCCGCCAGTCCATTGAAAAACGGGGTAAAGAAAGGCACATGAGGAGAATTATCCAGCATATTTAACGCCAGTCGCTGGATACCATCCGTAAATGC > NC_000913/2151486‑2151926
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
