Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F21 I0 R1 44 97.4 2221552 95.8% 2128246 141.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 2,532,298 A→T 100% T15T (ACA→ACT)  femA_3 → Aminoacyltransferase FemA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE2,532,2980AT100.0% 306.6 / ‑6.5 99T15T (ACA→ACT) femA_3Aminoacyltransferase FemA
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (45/52);  minor base G (1/0);  total (47/52)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  USA300TCH1516_ALE/2532164‑2532433
                                                                                                                                      |                                                                                                                                        
gtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTATCGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAg                                                                                                                                    <  1:276518/141‑1 (MQ=255)
   tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑t                                                                                                                                <  1:502235/141‑1 (MQ=255)
      gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑tttt                                                                                                                             <  2:1095658/141‑1 (MQ=255)
      gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑tttt                                                                                                                             >  1:589315/1‑141 (MQ=255)
      atAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGTACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑tttt                                                                                                                             <  2:674832/140‑1 (MQ=255)
       taataaGTTTATGATAAAATAAAAAAATAGGATTATTACTAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCACTGCGGTTACTACAAAGCA‑ttttt                                                                                                                            >  2:672660/1‑141 (MQ=255)
       tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGGTGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTAAAAAGCA‑ttttt                                                                                                                            >  2:399883/1‑141 (MQ=255)
       tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑ttttt                                                                                                                            >  1:677893/1‑141 (MQ=255)
        aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTc                                                                                                                           >  2:98125/1‑141 (MQ=255)
         aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTca                                                                                                                          >  2:406429/1‑141 (MQ=255)
               ttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCAAATTac                                                                                                                    >  1:646915/1‑141 (MQ=255)
               ttttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGATCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAACGCGTTTACTACAAATCA‑TTTTTCACATTac                                                                                                                    >  2:977401/1‑141 (MQ=255)
               ttttGATAAAATAAAAAAATAGCAATATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGGTGACTTCGGAGGAGTTCAATGCGTTTACTAGAAAGAA‑TTTTTCACATTac                                                                                                                    >  1:705164/1‑141 (MQ=255)
                tttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTaca                                                                                                                   <  2:873864/141‑1 (MQ=255)
                 ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGGA‑TTTTTCACATTacac                                                                                                                  <  1:546790/141‑1 (MQ=255)
                 ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacac                                                                                                                  <  1:215622/141‑1 (MQ=255)
                 ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacac                                                                                                                  <  1:110996/141‑1 (MQ=255)
                 ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacac                                                                                                                  <  1:401127/141‑1 (MQ=255)
                 ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacac                                                                                                                  <  1:198945/141‑1 (MQ=255)
                 ttGAAAAAATAAAAAAATAGCCTTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacac                                                                                                                  <  1:456588/141‑1 (MQ=255)
                  tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacaca                                                                                                                 <  1:158622/141‑1 (MQ=255)
                   gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacacac                                                                                                                >  2:890568/1‑141 (MQ=255)
                   gATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTacacac                                                                                                                >  2:712020/1‑141 (MQ=255)
                    ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTAcacaca                                                                                                               >  2:973210/1‑141 (MQ=255)
                          taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGc                                                                                                         <  2:1045747/141‑1 (MQ=255)
                          taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGc                                                                                                         <  2:1045387/141‑1 (MQ=255)
                           aaaaaaaTAGCATTATTAATATATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTTCACATTACACACAATCAGc                                                                                                         <  1:219798/141‑1 (MQ=255)
                              aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTAtt                                                                                                     <  1:148548/141‑1 (MQ=255)
                              aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTAtt                                                                                                     >  2:148958/1‑141 (MQ=255)
                              aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGCTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTAtt                                                                                                     <  1:767485/141‑1 (MQ=255)
                                aaTAGCATTATTAATAAATTCAACAATTATAGTTCAATTCAGAGTGGGATGGGAAAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCAATTCa                                                                                                   >  1:428908/1‑141 (MQ=255)
                                aaTAGCATTATTAATAAATTCAAAAATTATTGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTAAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACCATCAGCTATTCa                                                                                                   <  2:813080/141‑1 (MQ=255)
                                 aTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCAt                                                                                                  <  1:10900/141‑1 (MQ=255)
                                   aGCATTATTAATAAATTCACANANTAANGGCCAATTCAGCGAGGGATGGGATAAACTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTTCACATAATCAGCTATTCATTa                                                                                                <  1:567810/141‑1 (MQ=255)
                                            aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                       >  1:961333/1‑141 (MQ=255)
                                             ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTGCATACTGTTGCTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATagg                                                                                      >  1:525557/1‑140 (MQ=255)
                                             ataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATAATaga                                                                                      >  2:689508/1‑141 (MQ=255)
                                                aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACTTTGACTTCGGATGAGTTCCATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGTTATTCATTACAATCAtgacgtt                                                                                   >  2:141472/1‑135 (MQ=255)
                                                aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACCAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                   <  1:36620/141‑1 (MQ=255)
                                                aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                   <  1:682123/141‑1 (MQ=255)
                                                aaTTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGtt                                                                                   <  2:379578/141‑1 (MQ=255)
                                                   tCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGAt                                                                                <  1:18492/141‑1 (MQ=255)
                                                        aaTTATTGTTCAGTTCAGAGAGGGATGGGTTAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaaa                                                                           <  2:969290/141‑1 (MQ=255)
                                                         aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTTTCTTTTCATTCAACCAGAAAATCAATACAAGCAAATAAGTGATTTaaga                                                                          >  2:259888/1‑139 (MQ=255)
                                                         aTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACGACAAAGCA‑TTTTGCACATTACACACAATCAGCTATTCATTACAATCCTAGAGTTAATTCaaaa                                                                          >  2:757833/1‑141 (MQ=255)
                                                         aTTATAGTTCAATGCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TCTTTCCCATTACGCACAACCAGCTATTCATTACAATCATAGAGTTGATTTaaaa                                                                          >  2:500099/1‑141 (MQ=255)
                                                          ttATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAg                                                                         <  2:142596/141‑1 (MQ=255)
                                                            ataGTTCAATTCAGAGAGGGATGGGATAAAATAAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACACTCATAGAGCTGATTTCAAAGGc                                                                       >  1:947377/1‑141 (MQ=255)
                                                             taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:14158/141‑1 (MQ=255)
                                                             aaGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:663878/140‑1 (MQ=255)
                                                               gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                    >  1:377001/1‑141 (MQ=255)
                                                               gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                    >  1:869572/1‑141 (MQ=255)
                                                                 tCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAtgt                                                                  <  1:374559/141‑1 (MQ=255)
                                                                     ttCAGAGAGGGATGAGATAAAATGTACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAt                                                              <  1:90948/141‑1 (MQ=255)
                                                                         gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGCTGTACATCTTg                                                          >  2:592101/1‑141 (MQ=255)
                                                                         gagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGAGTTTACTAAAAAGCA‑TTTTTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAACTGGATGTGCATATTg                                                          >  2:274483/1‑141 (MQ=255)
                                                                         gagagGGATGGGATAAAATGAACTTTGTAACGTTGACATCGGATGAGTTCAATGCGTTTACAACAAAGCA‑TTTTTGACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGGGATGTGCATCTTg                                                          >  2:40131/1‑141 (MQ=255)
                                                                          agagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGTGATGTGCATCTTGt                                                         <  2:992029/141‑1 (MQ=255)
                                                                             gtgatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTCCACACAATCAGCTATTCATTACAATCATACAGGTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                      >  2:700219/3‑141 (MQ=255)
                                                                             gggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTGTACTACAAAGCA‑TTTTTAACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAgg                                                      >  2:294300/1‑141 (MQ=255)
                                                                              ggatgggatAAAATGAACTTTGTCACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                     <  1:379541/141‑1 (MQ=255)
                                                                              ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGAGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                     <  2:516938/141‑1 (MQ=255)
                                                                                atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  2:589315/141‑1 (MQ=255)
                                                                                atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:406429/141‑1 (MQ=255)
                                                                                 tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                  >  1:1102873/1‑141 (MQ=255)
                                                                                 tgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                  >  1:307944/1‑141 (MQ=255)
                                                                                 tgggatAAAATGAACTCTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGtt                                                  >  1:657932/1‑141 (MQ=255)
                                                                                  gggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTa                                                 >  2:822465/1‑141 (MQ=255)
                                                                                    gatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTaag                                               >  1:903061/1‑140 (MQ=255)
                                                                                    gatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATAACAATCATAGAGGTGATTTAAAAGGCGATGTGCATCTCGAAGGGGTTaaa                                               >  1:475914/1‑141 (MQ=255)
                                                                                            gAACTTTGTCACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAATGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACaa                                       >  2:708903/1‑141 (MQ=255)
                                                                                                    tAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAg                               <  1:3553/141‑1 (MQ=255)
                                                                                                        gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCACGTgct                           >  1:51985/1‑139 (MQ=255)
                                                                                                        gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                           >  1:517624/1‑141 (MQ=255)
                                                                                                         tttaCTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                          <  2:1056292/138‑1 (MQ=255)
                                                                                                         ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                          <  1:326169/141‑1 (MQ=255)
                                                                                                         ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                          <  2:815612/141‑1 (MQ=255)
                                                                                                         ttGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                          <  1:816550/141‑1 (MQ=255)
                                                                                                         ttGACTGCGGATGAGTTCAATGCGTTTACTACAAAGCC‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAtt                          <  1:1104085/141‑1 (MQ=255)
                                                                                                            aCTTCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCa                       <  2:634670/141‑1 (MQ=255)
                                                                                                              ttCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     <  2:185196/141‑1 (MQ=255)
                                                                                                              ttCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     <  1:170810/141‑1 (MQ=255)
                                                                                                              ttCGGATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAgg                     >  1:954622/1‑141 (MQ=255)
                                                                                                                 ggATGAGTTCAATGCGTTTGCTACAAAGCA‑TTTTTCAAATTACACACAATCAGCTATTCGTTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGATAAAGATGACAATGGTCAAGCGATTGCAGGATg                  >  2:616977/1‑141 (MQ=255)
                                                                                                                 ggATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATg                  >  1:211161/1‑141 (MQ=255)
                                                                                                                 ggATGAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGAAGGATg                  >  1:790492/1‑141 (MQ=255)
                                                                                                                     gAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa              <  1:712020/141‑1 (MQ=255)
                                                                                                                     gAGTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTa              <  1:890568/141‑1 (MQ=255)
                                                                                                                      aGTTCAATGCGTTTACTACAAAGCA‑TTTTTTTTTTTTTTTACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt             >  2:844119/1‑141 (MQ=255)
                                                                                                                      aGTTCAATGCGTTTACTACAAAGCA‑TTTTTCATTTTTTACACAATCAGCTATTCATTACAATCATAGAGATGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGTAGGGTGCGTAt             >  2:497284/1‑141 (MQ=255)
                                                                                                                       gTTCAATGCGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTCCAATCATAGAGTTGATTTCAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt            >  2:548123/1‑141 (MQ=255)
                                                                                                                            aTGCGTTTACTACAAAGTA‑TTTTTCACATTACACACCAGCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTCGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAg       <  1:672660/141‑1 (MQ=255)
                                                                                                                               cGTTTACTACAATGTA‑TGTTTCACATTACACAAAATGAGCTATTCATTACAATCATAGAGTTGATTCAAAAGGCGATGCGCATCTTGTAGAGGCTAAAGAAGACAATGGTCAAGTGATTTCAGGATGCTTATTGACAGAAg    <  2:946013/141‑1 (MQ=255)
                                                                                                                               cGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  1:12109/141‑1 (MQ=255)
                                                                                                                               cGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  1:53686/141‑1 (MQ=255)
                                                                                                                               cGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  2:401466/141‑1 (MQ=255)
                                                                                                                               cGTTTACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAg    <  2:315253/141‑1 (MQ=255)
                                                                                                                                gTTTACTACAAAGCA‑TTTTACACATTACACACACTCAGCTATTCATTACAATCCTAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCACGTGATTGCAGGATGCTTATTGACAGagga   >  1:517053/1‑138 (MQ=255)
                                                                                                                                 tttACTACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  >  1:692859/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                        
GTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCA‑TTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  USA300TCH1516_ALE/2532164‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A